A.

Hasil Pengamatan
1. Tabel Pengamatan
a. Pembuatan Medium Disolusi
Nama Bahan
Larutan Stok A
Larutan Stok B
b. Pembuatan Kurva Baku danPersamaan Regresi
Konsentrasi (ppm)
5
10
Absorbansi
0,209
0,363
c. Tabel Uji Disolusi
1) Nilai Absorbansi Sampel Uji Disolusi
Waktu (menit)
Absorbansi Labu

Jumlah
26,825 gram
13,9 gram

15
0,501

20
0,653

25
0,802

10

20

30

45

60

0,246

0,363

0,502

0,646

0,643

0,634

0,247

0,364

0,549

0,649

0,644

0,634

0,251

0,309

0,549

0,648

0,644

0,630

0,238

0,367

0,505

0,643

0,639

0,640

0,245

0,362

0,510

0,644

0,640

0,634

0,249
6 (ppm)
2) Konsentrasi Uji Disolusi

0,349

0,507

0,642

0,640

0,636

1 (ppm)
Absorbansi Labu
2 (ppm)
Absorbansi Labu
3 (ppm)
Absorbansi Labu
4 (ppm)
Absorbansi Labu
5 (ppm)
Absorbansi Labu

Waktu
(menit)
Konsentrasi

10

20

30

45

60

6,195

9,796

15,493

19,72

19,60

19,415

3) Konsentrasi (Faktor Koreksi) Sampel Uji Disolusi

Waktu (menit)
Konsentrasi

10

20

30

45

60

(FaktorKoreksi)

6,195

9,83

15,501

19,89

19,88

19,84

(ppm)

4) Persentase Terdisolusi Tablet Metoklopramid

Waktu
(menit)
%
Terdisolusi

10

20

30

45

60

5,575

8,847

13,951

17,9

17,89

17,85

2. Perhitungan
a. Pembuatan Buffer fosfat pH 6,8
-Stok A : timbang 17,799 g Na 2HPO4 , dimasukkan ke labu takar 1 liter,
ditambahkan aquades labu, dan dihomogenkan, tambah lagi aquades sampai
batas.
-Stok B : timbang 15,601 g NaH 2PO4 , dimasukkan ke labu takar 1 liter,
ditambahkan aquades 1/4 labu, dan dihomogenkan, tambah lagi aquades sampai
tanda batas.
-Standar pembuatan buffer fosfat pH 6,8 dibutuhkan sebanyak 100 ml. (49,1
mL Na2HPO4 + 50,9 mL NaH2PO4). Dibuat media disolusi sebanyak 6,2 L.
-Larutan medium 6 tabung disolusi: 6 x 900 mL = 54000 mL.
-Larutan pengganti cuplikan : 5 mL x 6 waktu x 6 tabung disolusi = 180 mL =
200 mL.
-Larutan Stok 50 mL.
-Larutan Seri Konsentrasi : 5 seri konsentrasi + 1 blanko(@10 mL) maka, 6 x
10 mL = 60 mL = 100 mL.
-Jumlah total medium = 5400 mL + 200 mL + 500 mL + 100 mL = 6200 mL.
Stok A

Stok B

62 = 6200 m
100mL
mLx 62 = 3044,2 mL = 3050xmL
Stok A : 49,1
Stok B : 50,9 mL x 62 = 3155,8 mL = 3200 mL
1) Na2HPO4
53,65
X
=
1000 mL 500 mL
2) NaH2PO4
27,89
X
=
1000 ml 500 mL

= 26,825 g

X
= 13,9 g
3) Aquades
6250 mL.
b. Pembuatan stok metaklopramid 100 ppm dalam 500 mL.
0,5 L x 100 ppm = 50 mg
c. Pembuatan Larutan Seri Konsentrasi Stok
1) 5 ppm
M1 V1
= M2V2
100 ppm V1 = 5 ppm 10 mL
V1 = 0,5 mL
2) 10 ppm
M1 VI
= M2 V2
100 ppm V1 = 10 ppm 10 mL
V1 = 1 mL
3) 15 ppm
M1 V1
= M2V2
100 ppm V1 = 15 ppm 10 mL
V1 = 1,5 mL
4) 20 ppm
M1VI
= M2 V2
100 ppm V1 = 10 ppm 10 mL
V1 = 2 mL
5) 25 ppm
M1VI
= M2 V2
100 ppm V1 = 25 ppm100 mL
V1 = 2.5 mL
d. Penentuan Kurva Baku
a = 0,0628
b = 0,02952
y = a + bz
r = 0,999
e. Konsentrasi dari Absorbansi
5 menit (1)
y = 0,0628 + 0,02952 x
0,246 = 0,0628 + 0,02952 x
0,246 - 0,0628
x=
0,02952

5 menit (2)
y = 0,0628 + 0,02952 x
0,247 = 0,0628 + 0,02952 x
0,247-0,0628
x=
0,029 5 2

= 6,20 ppm

= 6,23 ppm

5 menit (3)
y = 0,0628 + 0,02952 x
0,251 = 0,0628 + 0,02952 x
x=

0,251-0,0628
0,029 5 2

5 menit (4)
y = 0,0628 + 0,02952 x
0,238 = 0,0628 + 0,02952 x
x=

0,238-0,0628
0,029 5 2

= 6,34 ppm
5 menit (5)
y = 0,0628 + 0,02952 x
0,245 = 0,0628 + 0,02952 x
0,245-0,0628
x=
0,029 5 2

= 5,93 ppm
5 menit (6)
y = 0,0628 + 0,02952 x
0,249 = 0,0628 + 0,02952 x
0,249-0,0628
x=
0,029 5 2

= 6,17 ppm

= 6,30 ppm

10 menit (1)
y = 0,0628 + 0,02952 x
0,363 = 0,0628 + 0,02952 x
0,363-0,0628
x =
0,0295 2

10 menit (2)
y = 0,0628 + 0,02952 x
0,363 = 0,0628 + 0,02952 x
0,363-0,0628
x =
0,0295 2

= 10,16 ppm

= 10,16 ppm

10 menit (3)
y = 0,0628 + 0,02952 x
0,309 = 0,0628 + 0,02952 x
0,309-0,0628
x=
0,029 5 2

10 menit (4)
y = 0,0628 + 0,02952 x
0,367 = 0,0628 + 0,02952 x
0,367-0,0628
x=
0,029 5 2

= 8,34 ppm

= 10,30 ppm

10 menit (5)
y = 0,0628 + 0,02952 x
0,362 = 0,0628 + 0,02952 x
0,362-0,0628
x=
0,029 5 2

10 menit (6)
y = 0,0628 + 0,02952 x
0,349 = 0,0628 + 0,02952 x
0,349-0,0628
x=
0,029 5 2

= 10,13 ppm

= 9,69 ppm

20 menit (1)
y = 0,0628 + 0,02952 x
0,502 = 0,0628 + 0,02952 x
0,502 - 0,0628
x=
0,02952

20 menit (2)
y = 0,0628 + 0,02952 x
0,549 = 0,0628 + 0,02952 x
0,549 - 0,0628
x=
0,02952

= 14,87 ppm

= 16,47 ppm

20 menit (3)
y = 0,0628 + 0,02952 x
0,549 = 0,0628 + 0,02952 x
0,549 - 0,0628
x=
0,02952

20 menit (4)
y = 0,0628 + 0,02952 x
0,505 = 0,0628 + 0,02952 x
0,505-0,0628
x=
0,029 5 2

= 16,47 ppm

= 14,97 ppm

20 menit (5)
y = 0,0628 + 0,02952 x
0,510 = 0,0628 + 0,02952 x
0,510-0,0628
x=
0,029 5 2

20 menit (6)
y = 0,0628 + 0,02952 x
0,507 = 0,0628 + 0,02952 x
0,507-0,0628
x=
0,029 5 2

= 15,14 ppm

= 15,04 ppm

30 menit (1)
y = 0,0628 + 0,02952 x
0,646 = 0,0628 + 0,02952 x
0,646 - 0,0628
x=
0,02952

30 menit (2)
y = 0,0628 + 0,02952 x
0,649 = 0,0628 + 0,02952 x
0,649-0,0628
x=
0,029 5 2

= 19,75 ppm

= 19,85 ppm

30 menit (3)
y = 0,0628 + 0,02952 x
0,648 = 0,0628 + 0,02952 x
0,648-0,0628
x=
0,029 5 2

30 menit (4)
y = 0,0628 + 0,02952 x
0,643 = 0,0628 + 0,02952 x
0,643 - 0,0628
x=
0,02952

= 19,82 ppm

= 19,65 ppm

30 menit (5)
y = 0,0628 + 0,02952 x
0,644 = 0,0628 + 0,02952 x
0,644-0,0628
x=
0,029 5 2

30 menit (6)
y = 0,0628 + 0,02952 x
0,642 = 0,0628 + 0,02952 x
0,642-0,0628
x=
0,029 5 2

= 19,68 ppm

= 19,62 ppm

45 menit (1)
y = 0,0628 + 0,02952 x
0,643 = 0,0628 + 0,02952 x
0,643 - 0,0628
x=
0,02952

45 menit (2)
y = 0,0628 + 0,02952 x
0,644 = 0,0628 + 0,02952 x
0,644 - 0,0628
x=
0,02952

= 19,65 ppm

= 19,65 ppm

45 menit (3)
y = 0,0628 + 0,02952 x
0,644 = 0,0628 + 0,02952 x
0,644 - 0,0628
x=
0,02952

45 menit (4)
y = 0,0628 + 0,02952 x
0,639 = 0,0628 + 0,02952 x
0,639-0,0628
x=
0,029 5 2

= 19,68 ppm

= 19,51 ppm

45 menit (5)
y = 0,0628 + 0,02952 x
0,640 = 0,0628 + 0,02952 x
0,640-0,0628
x=
0,029 5 2

45 menit (6)
y = 0,0628 + 0,02952 x
0,640 = 0,0628 + 0,02952 x
0,640 - 0,0628
x=
0,02952

= 19,55 ppm

= 19,55 ppm

60 menit (1)
y = 0,0628 + 0,02952 x
0,634 = 0,0628 + 0,02952 x
0,634 - 0,0628
x=
0,02952

60 menit (2)
y = 0,0628 + 0,02952 x
0,634 = 0,0628 + 0,02952 x
0,634 - 0,0628
x=
0,02952

= 19,34 ppm

= 19,34 ppm

60 menit (3)
y = 0,0628 + 0,02952 x
0,630 = 0,0628 + 0,02952 x
0,630-0,0628
x=
0,029 5 2

60 menit (4)
y = 0,0628 + 0,02952 x
0,640 = 0,0628 + 0,02952 x
0,640-0,0628
x=
0,029 5 2

= 19,49 ppm

= 19,5 ppm

60 menit (5)
y = 0,0628 + 0,02952 x
0,634 = 0,0628 + 0,02952 x
0,634 - 0,0628
x=
0,02952

5 menit (1)
y = 0,0628 + 0,02952 x
0,636 = 0,0628 + 0,02952 x
0,636 - 0,0628
x=
0,02952

= 19,34 ppm

= 19,4 ppm

f. Faktor Koreksi
1) A
a) A(1) = 6,20 ppm
b) A(2) =

6,293 ppm +

6,20 ) = 6,243 ppm

(5mL
900mL

6,34 ppm +

c) A(3) =

( 6,20+6,293 ) )= 6,409 ppm

(5mL
900mL

6 ,293 ppm +

d) A(4) =

6,172 ppm +

e) A(5) =

( 6,20+6,293+6,34 )) = 6,034 ppm

(5mL
900mL

( 6,20+6,293+6,34+5,93 ) )= 6,309 ppm

(5mL
900mL

f) A(6) =
6,307 ppm +

( 6,20+6,293+6,34+5,93+6,172 )) = 6,309 ppm

(5mL
900mL

2) B
a) B(1) = 10,169 ppm
10,169 ppm +

b) B(2) =
c) B(3) =
d) B(4) =

10,169 ) = 10,22 ppm

(5mL
900mL

8,340 ppm +

(10,169+10,169) ) = 8,45 ppm

(5mL
900mL

10,304 ppm +

(10,169+10,169+8,340) ) = 10,463 ppm

(5mL
900mL

e) B(5) =
10,133 ppm +

f) B(6) =

(10,169+10,169+8,340+10,304 )= 10,34 ppm

( 5mL
900mL

9,695 ppm +

(10,169+10,169+8,340+10,304+10, 133 )
(5mL
900mL

9,967 ppm
3) C
a) C(1) = 14,878 ppm
b) C(2) =
c) C(3) =
d) C(4) =

16,470 ppm +

14,878 ) = 16,55 pp m
(5mL
900mL

16,478 ppm +

(14,878+16,470) ) = 16,652 ppm

(5mL
900mL

14,979 ppm +

(14,878+16,470+16,478) ) = 15,244 ppm

(5mL
900mL

e) C(5) =

15,149 ppm +

(14,878+16,470+16,478+14,979) )
(5mL
900mL

15,395 ppm
f) C(6) =

15,047 ppm+

(14,878+16,470+16,478+14,979+15,149) )
(5mL
900mL

15,395 ppm

4) D
a) D(1) = 19,756
b) D(2) =
c) D(3) =
d) D(4) =

19,875 ppm +

19,756 ) = 19,966 ppm

(5mL
900mL

19,823 ppm +

( 19,756+19,875 )) = 20,04 ppm

(5mL
900mL

19,654 ppm +

( 19,756+19,875+19,823 )) = 19,984 ppm

(5mL
900mL

e) D(5) =
19,688 ppm +

f) D(6) =

( 19,756+19,875+19,823+19,654 ) )= 20,12 ppm

(5mL
900mL

19,620 ppm +

( 19,756+19,875+19,823+19,654+19,688 ))
(5mL
900mL

20,12 ppm
5) E
a) E(1) = 19,654 ppm
b) E(2) =
c) E(3) =
d) E(4) =
e) E(5) =

19,688 ppm +

19,654 ) = 19,76 ppm

(5mL
900mL

19,688 ppm +

(19,654+19,688) ) = 19,906 ppm

(5mL
900mL

19,688 ppm +

(19,654+19,688+19,688) ) = 19,79 ppm

(5mL
900mL

19,688 ppm +

( 19,654+19,688+19,688+19,464 ) )
(5mL
900mL

19,958 ppm

f) E(6) =

19,688 ppm +

( 19,654+19,688+19,688+19,464+19 ,552 ) )
( 5mL
900mL

19,958 ppm
6) F
a) F(1) = 19,349 ppm
b) F(2) =
c) F(3) =

d) F(4) =

19,349 ppm +

19,349 ) = 19,45 ppm

(5mL
900mL

19,214 ppm +

(19,349+19,349) ) = 19,428 ppm

(5mL
900mL

19,552 ppm +

( 19,349+19,349+19,214 ) )= 19,87 ppm

(5mL
900mL

e) F(5) =
19,349 ppm +

f) F(5) =

( 19,349+19,349+19,214+19,522 ) ) = 19,77 ppm

(5mL
900mL

19,417 ppm +

( 19,349+19,349+19,214+19,522+19,49 ))
( 5mL
900mL

19,95 ppm

g. Perhitungan persen (%) disolusi

100mg 100mg
=
=111,11 ppm
900mL 0,9L
1) A =

5,2505
100%=4,72 %
111,11

2) B =

9,933
100%=8,93 %
111,11

15,69
100%=14,12 %
3) C = 111,11

16,711
100%=15,04 %
4) D = 111,11
19,86
100%=17,87 %
5) E = 111,11
16,41
100%=8,93 %
6) F = 111,11

3. Grafik
a. Kurva Baku dan Persamaan Regresi

KURVA KALIBRASI

ABSORBANSI

1
0.9
0.8
0.7
0.6
0.5
0.4
0.30.21
0.2
0.1
0
5

0.8
0.65
0.5
0.36

10

15
KONSENTRASI

b. Persen Disolusi

20

25

KURVA TERDISOLUSI
20.5
18.5
16.5

17.9

17.89

17.85

30

45

60

13.95

14.5
12.5
% Terdisolusi 10.5
8.5
5.58
6.5

8.85

4.5
2.5
0.5
5

10

20

Waktu (menit)