DAFTAR ISI

Halaman
HALAMAN JUDUL........................................................................................ i
LEMBAR PENGESAHAN ............................................................................. ii
DAFTAR ISI .................................................................................................... iii
DAFTAR GAMBAR ....................................................................................... vi
DAFTAR TABEL ............................................................................................ vii
DAFTAR LAMPIRAN .................................................................................... vii
DAFTAR SINGKATAN ................................................................................. ix
BAB I PENDAHULUAN
1.1 Latar Belakang ........................................................................................ 1
1.2 Rumusan Masalah ................................................................................... 2
1.3 Tujuan ..................................................................................................... 3
1.3.1 Tujuan umum .............................................................................. 3
1.3.2 Tujuan Khusus ............................................................................ 3
1.4 Manfaat ................................................................................................... 3
1.4.1 Manfaat Ilmiah............................................................................ 3
1.4.2 Manfaat Praktis ........................................................................... 3
BAB II TINJAUAN PUSTAKA
2.1 Keputihan ................................................................................................ 4
2.1.1 Definisi Keputihan ...................................................................... 4
2.1.2 Etiologi ....................................................................................... 4
2.1.3 Patogenesis ................................................................................. 5
2.1.4 Gejala Klinis ............................................................................... 6
2.1.5 Komplikasi.................................................................................. 6
2.2 Kandidiasis ............................................................................................. 6
2.3 Candida Albicans.................................................................................... 7
2.3.1 Morfologi .................................................................................... 7
2.3.2 Taksonomi .................................................................................. 8
2.3.3 Patogenitas .................................................................................. 9
2.4 Tumbuhan Sasating (Cassia alata Linn.) ............................................... 10
2.4.1 Uraian Tumbuhan Sasating (Cassia alata Linn.) ....................... 10
2.4.2 Nama Daerah Tumbuhan Sasating (Cassia alata Linn.) ............ 11
2.4.3 Taksonomi Tumbuhan Sasating (Cassia alata Linn.) ................ 11
2.4.4 Kandungan Senyawa Kimia Tumbuhan Sasating
(Cassia alata Linn.) .................................................................... 11
2.5 Ekstraksi ................................................................................................. 15
2.5.1 Defenisi ....................................................................................... 15
2.5.2 Metode Ekstraksi ........................................................................ 16
2.6 Uji Aktivitas Antifungi ........................................................................... 17
BAB III LANDASAN TEORI DAN HIPOTESIS
3.1 Landasan Teori ....................................................................................... 19
3.2 Kerangka Teori ....................................................................................... 20
3.3 Kerangka Konsep.................................................................................... 21
3.4 Hipotesis ................................................................................................. 22

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BAB IV METODE PENELITIAN
4.1 Jenis dan Rancangan Penelitian .............................................................. 23
4.2 Sampel dan Teknik Pengambilan Sampel .............................................. 23
4.3 Estimasi Besar Sampel ........................................................................... 23
4.4 Kriteria Pemilihan (Inklusi dan Eksklusi) .............................................. 24
4.5 Variabel Penelitian.................................................................................. 24
4.6 Definisi Operasional ............................................................................... 24
4.6.1 Pertumbuhan koloni Candida albicans ...................................... 24
4.6.2 Ekstrak etanol akar sasating........................................................ 24
4.7 Alat dan Bahan Penelitian ...................................................................... 24
4.7.1 Alat ............................................................................................. 24
4.7.2 Bahan .......................................................................................... 25
4.8 Prosedur Pengambilan atau Pengumpulan Data ..................................... 25
4.8.1 Identifikasi Tanaman .................................................................. 25
4.8.2 Prosedur Pengambilan Akar Sasating ......................................... 25
4.8.3 Pembuatan Ekstrak Etanol Akar Sasating .................................. 26
4.8.4 Pengujian Kandungan Senyawa Metabolit Sekunder ................. 27
4.8.5 Pengujian Aktivitas Antifungi .................................................... 29
4.9 Cara Pengolahan Data dan Teknil Analisis Data .................................... 32
4.9.1 Pengolahan data .......................................................................... 32
4.9.2 Analisis Data ............................................................................... 32
4.10 Tempat dan Waktu Penelitian................................................................. 32
DAFTAR PUSTAKA
LAMPIRAN

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 DAFTAR GAMBAR
Halaman
Gambar 2.1 Struktur Dinding Sel Candida albicans ....................................... 8
Gambar 2.2 Tumbuhan Sasating ...................................................................... 11
Gambar 2.3 Akar Sasating ............................................................................... 11
Gambar 2.4 Struktur Kimia Tanin ................................................................... 12
Gambar 2.5 Kerangka Flavonoid ..................................................................... 13
Gambar 3.1 Kerangka Teori ............................................................................. 20
Gambar 3.2 Kerangka Konsep ......................................................................... 21
Gambar 4.1 Lempeng Mueller-Hinton sebagai tempat tumbuhnya
Candida albicans........................................................................... 31
Gambar 4.2 Alur penelitian .............................................................................. 33

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 DAFTAR TABEL
Halaman
Tabel 4.1 Jadwal Penelitian.............................................................................. 34
Tabel 4.2 Rincian Biaya Penelitian .................................................................. 35

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 DAFTAR LAMPIRAN
Halaman
Lampiran 1 Hasil Determinasi Tumbuhan Sasating (Cassia alata Linn.) ....... 39
Lampiran 2 Kuesioner ..................................................................................... 40
Lampiran 3 Rekapitulasi Hasil Uji Pendahuluan ............................................. 44
Lampiran 4 Prosedur Kerja Penelitian ............................................................. 47
Lampiran 5 Langkah Uji Fitokimia.................................................................. 55

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 DAFTAR SINGKATAN

m : Micron
AIDS : Acquired Immune Deficiency Syndrome
ATP : Adenosine Triphosphate
BaCl2 : Barium Chloride
BHI : Brain Heart Infusion
C : Celcius
CFU : Colony Forming Unit
CR3 : Complement Receptor
DMSO : Dimethyl sulfoxide
DNA : Deoxyribonucleic acid
gr : Gram
H2SO4 : Asam Sulfat
HIV : Human Immunodeficiency Virus
Kg : Kilo Gram
m : Meter
MHA : Muller Hinton Agar
mL : Mililiter
NaCl : Natrium Chloride
NCCLS : Nation Committee for Clinical Laboratory Standards
OH : Hidroksida
pH : Potential Of Hydrogen
RNA : Ribonucleic acid
SDA : Sabouraud Dextrose Agar
SDA : Sabouraud Dextrose Agar
WHO : World Health Organitation

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