a.

Kurva baku Teofilin

Absorbansi
Konsentrasi Rata-rata
1 2 3
4 ppm 0,21012 0,2091 0,2089 0,2094
6 ppm 0,3198 0,3209 0,3219 0,32086667
8 ppm 0,4218 0,4218 0,4214 0,42166667
10 ppm 0,5173 0,5153 0,5159 0,51616667
12 ppm 0,6336 0,6363 0,6366 0,6355

Kurva Baku Teofilin

0.7
0.6
y = 0,1048x + 0,0017
0.5
R² = 0,9987
absorbansi

0.4
0.3 Rata-rata
0.2 Linear (Rata-rata)
0.1
0
0 2 4 6 8
konsentrasi

b. Sampel a (λ 272 nm)

Persamaan kurva baku : y = 0,1048x + 0,0017
Volume media : 900 ml (aquades)
Vol sampel tiap pengambilan : 5 ml
Konsentrasi tablet awal : 300 mg = 300.000 µg

Absorbansi Rata-rata %
Waktu Konsentrasi
1 2 (absorbansi) disolusi
5 menit 0,0524 0,0564 0,0544 0,50286 0,151%
10 menit 0,2550 0,2471 0,25105 2,37929 0,715%
 20 menit 0,2756 0,2762 0,2759 2,61641 0,789%
30 menit 0,3242 0,3249 0,32455 3,08063 0,924%
45 menit 0,5546 0,5527 0,55365 5,2667 1,585%
60 menit 1,9515 1,9522 1,95185 18,6083 5,591%

1. Pada menit ke-5

 Absorbansi 0,0544
y = 0,1048x + 0,0017
0,0544 = 0,1048x + 0,0017
0,1048x = 0,0544 - 0,0017
X = 0,50286 µg /ml
 Konsentrasi x vol media = 0,50286 µg /ml x 900 ml = 452,574 µg
5 𝑚𝑙
 FK5 = x 452,574 µg = 2,5143 µg
900 𝑚𝑙
452,574 µg
 % disolusi = x 100% = 0,151%
300.000 µg

2. Pada menit ke-10

 Absorbansi 0,25105
y = 0,1048x + 0,0017
0,25105 = 0,1048x + 0,0017
0,1048x = 0,25105 - 0,0017
X = 2,37929 µg /ml
 Konsentrasi x vol media = 2,37929 µg /ml x 900 ml = 2141,361 µg
 Kadar total = 2141,361 µg + 2,5143 µg = 2143,8753 µg
5 𝑚𝑙
 FK5 = x 2143,8753 µg = 11,9104 µg
900 𝑚𝑙
2143,8753 µg
 % disolusi = x 100% = 0,715%
300.000 µg

3. Pada menit ke-20

 Absorbansi 0,2759
 y = 0,1048x + 0,0017
0,2759 = 0,1048x + 0,0017
0,1048x = 0,2759 - 0,0017
X = 2,61641 µg /ml
 Konsentrasi x vol media = 2,61641 µg /ml x 900 ml = 2354,769 µg
 Kadar total = 2354,769 µg + 11,9104 µg = 2366,6794 µg
5 𝑚𝑙
 FK5 = x 2366,6794 µg = 13,1482 µg
900 𝑚𝑙
2366,6794 µg
 % disolusi = x 100% = 0,789%
300.000 µg

4. Pada menit ke-30

 Absorbansi 0,32455
y = 0,1048x + 0,0017
0,32455 = 0,1048x + 0,0017
0,1048x = 0,32455 - 0,0017
X = 3,08063 µg /ml
 Konsentrasi x vol media = 3,08063 µg /ml x 900 ml = 2772,567 µg
 Kadar total = 2772,567 µg + 13,1482 µg = 2785,7152 µg
5 𝑚𝑙
 FK5 = x 2785,7152 µg = 15,4761 µg
900 𝑚𝑙
2772,567 µg
 % disolusi = x 100% = 0,924%
300.000 µg

5. Pada menit ke-45

 Absorbansi 0,55365
y = 0,1048x + 0,0017
0,55365 = 0,1048x + 0,0017
0,1048x = 0,55365 - 0,0017
X = 5,2667 µg /ml
 Konsentrasi x vol media = 5,2667 µg /ml x 900 ml = 4740,03 µg
 Kadar total = 4740,03 µg + 15,4761 µg = 4755,5061 µg
 5 𝑚𝑙
 FK5 = x 4755,5061 µg = 26,4194 µg
900 𝑚𝑙
4755,5061 µg
 % disolusi = x 100% = 1,585%
300.000 µg

6. Pada menit ke-60

 Absorbansi 1,95185
y = 0,1048x + 0,0017
1,95185 = 0,1048x + 0,0017
0,1048x = 1,95185 - 0,0017
X = 18,6083 µg /ml
 Konsentrasi x vol media = 18,6083 µg /ml x 900 ml = 16747,47 µg
 Kadar total = 16747,47 µg + 26,4194 µg = 16773,8894 µg
5 𝑚𝑙
 FK5 = x 16773,8894 µg = 93,1883 µg
900 𝑚𝑙
16773,8894 µg
 % disolusi = x 100% = 5,591%
300.000 µg

c. Sampel b (λ 727 nm)

Persamaan kurva baku : y = 0,1048x + 0,0017
Volume media : 900 ml (aquades)
Vol sampel tiap pengambilan : 5 ml
Konsentrasi tablet awal : 300 mg = 300.000 µg

Absorbansi Rata-rata %
Waktu Konsentrasi
1 2 (absorbansi) disolusi
5 menit 0,4550 0,4533 0,45415 4,31727 1,295%
10 menit 1,0242 1,0207 1,02245 9,73998 2,929%
20 menit 1,5210 1,4976 1,5093 14,3855 4,332%
30 menit 1,9166 1,8276 1,8721 17,84732 5,378%
45 menit 1,6112 1,6405 1,62585 15,49761 4,679%
60 menit 4,4714 2,9517 3,71155 35,39933 10,646%
1. Pada menit ke-5
 Absorbansi 0,45415
y = 0,1048x + 0,0017
0,45415 = 0,1048x + 0,0017
0,1048x = 0,45415 - 0,0017
X = 4,31727 µg /ml
 Konsentrasi x vol media = 4,31727 µg /ml x 900 ml = 3885,543 µg
5 𝑚𝑙
 FK5 = x 3885,543 µg = 21,5863 µg
900 𝑚𝑙
3885,543 µg
 % disolusi = x 100% = 1,295%
300.000 µg

2. Pada menit ke-10

 Absorbansi 1,02245
y = 0,1048x + 0,0017
1,02245 = 0,1048x + 0,0017
0,1048x = 1,02245 - 0,0017
X = 9,73998 µg /ml
 Konsentrasi x vol media = 9,73998 µg /ml x 900 ml = 8765,982 µg
 Kadar total = 8765,982 µg + 21,5863 µg = 8787,5683 µg
5 𝑚𝑙
 FK5 = x 8787,5683 µg = 48,8198 µg
900 𝑚𝑙
8787,5683 µg
 % disolusi = x 100% = 2,929%
300.000 µg

3. Pada menit ke-20

 Absorbansi 1,5093
y = 0,1048x + 0,0017
1,5093 = 0,1048x + 0,0017
0,1048x = 1,5093 - 0,0017
X = 14,3855 µg /ml
 Konsentrasi x vol media = 14,3855 µg /ml x 900 ml = 12946,95 µg
  Kadar total = 12946,95 µg + 48,8198 µg = 12995,7698 µg
5 𝑚𝑙
 FK5 = x 12995,7698 µg = 72,1987 µg
900 𝑚𝑙
12995,7698 µg
 % disolusi = x 100% = 4,332%
300.000 µg

4. Pada menit ke-30

 Absorbansi 1,8721
y = 0,1048x + 0,0017
1,8721 = 0,1048x + 0,0017
0,1048x = 1,8721 - 0,0017
X = 17,84732 µg /ml
 Konsentrasi x vol media = 17,84732 µg /ml x 900 ml = 16062,588 µg
 Kadar total = 16062,588 µg + 72,1987 µg = 16134,7867 µg
5 𝑚𝑙
 FK5 = x 16134,7867 µg = 89,6377 µg
900 𝑚𝑙
16134,7867 µg
 % disolusi = x 100% = 5,378%
300.000 µg

5. Pada menit ke-45

 Absorbansi 1,62585
y = 0,1048x + 0,0017
1,62585 = 0,1048x + 0,0017
0,1048x = 1,62585 - 0,0017
X = 15,49761 µg /ml
 Konsentrasi x vol media = 15,49761 µg /ml x 900 ml = 13947,849 µg
 Kadar total = 13947,849 µg + 89,6377 µg = 14037,4867 µg
5 𝑚𝑙
 FK5 = x 14037,4867 µg = 77,986 µg
900 𝑚𝑙
14037,4867 µg
 % disolusi = x 100% = 4,679%
300.000 µg
6. Pada menit ke-60
 Absorbansi 3,71155
y = 0,1048x + 0,0017
3,71155 = 0,1048x + 0,0017
0,1048x = 3,71155 - 0,0017
X = 35,39933 µg /ml
 Konsentrasi x vol media = 35,39933 µg /ml x 900 ml = 31859,379 µg
 Kadar total = 31859,379 µg + 77,986 µg = 31937,383 µg
5 𝑚𝑙
 FK5 = x 31937,383 µg = 177,4299 µg
900 𝑚𝑙
31937,383 µg
 % disolusi = x 100% = 10,646%
300.000 µg