Badzlina Dwi Ningsih

240210130077

TUGAS INDIVIDU BIOPROSES

PROBLEMS
6.1 A simple, batch fermentation of an aerobic bacterium growing on methanol
gave the results shown in the table. Calculate:
a) Maximum growth rate (𝜇max)
b) Yield on substrate (Yx/s)
c) Mass doubling time (td)
d) Saturation constant (Ks)
e) Specific growth rate (𝜇net) at t = 10 h

Answer:
a) Maximum growth rate (𝝁max):

Laju pertumbuhan maksimum antara t=10 dan t=12

𝑋2 = 𝑋1 𝑒 𝜇∆𝑡 (1)
𝑙𝑛𝑋2 = (𝑙𝑛𝑋1 )(𝑙𝑛𝑒 𝜇∆𝑡 )
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240210130077

𝑥2
ln = 𝜇∆𝑡
𝑥1
3,2
ln = 𝜇 (12 − 10)
1,77
0,592
𝜇=( )
2
𝝁𝒎𝒂𝒙 = 𝟎, 𝟐𝟗𝟔 𝒉𝒓−𝟏

b) Yield on substrate (Yx/s)

Dari tabel:
𝑋𝑜 = 0,2ℎ𝑟 −1 𝑋𝑓 = 6,2ℎ𝑟 −1
𝑆𝑜 = 9,23ℎ𝑟 −1 𝑆𝑓 = 0 ℎ𝑟 −1
𝑥𝑓 − 𝑥𝑜
𝑌𝑥/𝑠 =
𝑆𝑜 − 𝑆𝑓
6,2 − 0,2
𝑌𝑥/𝑠 =
9,23 − 0
𝒀𝒙/𝒔 = 𝟎, 𝟔𝟓

c) Mass doubling time (td)

Mass doubling time hanya berlangsung pada fase pertumbuhan eksponesial.
Diketahui:
Laju pertumbuhan maksimum = 0,296 ℎ𝑟 −1
ln 2
𝑡𝑑 =
𝜇𝑚𝑎𝑥
ln 2
𝑡𝑑 =
0,296
𝒕𝒅 = 𝟐, 𝟑𝟒 𝒉𝒓

d) Saturation constant (Ks)

Nilai terletak diantara 3 poin terakhir dimana substrat mengalami penurunan
(habis), yaitu antara S = 0,0 gl-1 dan S = 0,92 gl-1
Ks = (0 + 0,92)/2
= 0,46
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240210130077

e) Specific growth rate (𝝁net) at t = 10 h

Laju pertumbuhan spesifik terjadi dipertengahan proses fermentasi, maka
nilainya sama dengan laju pertumbuhan maksimum ( μmax ) = 0,292 hr-1