DAFTAR ISI

Halaman
HALAMAN JUDUL ...................................................................................... ii
KATA PENGANTAR .................................................................................... iii
LEMBAR PENGESAHAN ........................................................................... v
DAFTAR ISI .................................................................................................. vi
DAFTAR TABEL .......................................................................................... viii
DAFTAR GAMBAR ..................................................................................... ix
DAFTAR LAMPIRAN. ................................................................................. x
DAFTAR SINGKATAN DAN LAMBANG ................................................ xi
BAB 1. PENDAHULUAN ............................................................................. 1
1.1 Latar Belakang ................................................................................ 1
1.2 Rumusan Masalah ........................................................................... 3
1.3 Tujuan ............................................................................................. 3
1.4 Manfaat ........................................................................................... 3
BAB 2. TINJAUAN PUSTAKA .................................................................... 4
2.1 Sistem Reproduksi Sapi .................................................................. 4
2.1.1 Sistem Reproduksi Sapi Jantan ........................................... 4
2.1.2 Sistem Reproduksi Sapi Betina ........................................... 6
2.2 Brucellosis ...................................................................................... 9
2.2.1 Etiologi Brucellosis ............................................................ 6
2.2.2 Epidemiologi Brucellosis ................................................... 10
2.2.3 Gejala Klinis Brucellosis .................................................... 12
2.2.4 Patogenesa Brucellosis ....................................................... 13
2.2.5 Diagnosa Brucellosis .......................................................... 14
2.2.5.1 Pemeriksaan Bakteriologik ................................... 14
2.2.5.2 Pemeriksaan Serologik .................................... ...... 15
2.2.5.3 Uji Tambahan ........................................................ 18
2.2.6 Pencegahan, Pengendalian dan Pemberantasan
Brucellosis ......................................................................... 21
2.3 Infectious Bovine Rhinotracheitis (IBR) ........................................ 22
2.3.1 Etiologi Infectious Bovine Rhinotracheitis (IBR) .............. 22
2.3.2 Epidemiologi Infectious Bovine Rhinotracheitis (IBR) ..... 23
2.3.3 Gejala Klinis Infectious Bovine Rhinotracheitis (IBR) ...... 24
2.3.4 Patogenesa Infectious Bovine Rhinotracheitis (IBR) ......... 27
2.3.5 Diagnosa Infectious Bovine Rhinotracheitis (IBR) ............ 27
2.3.6 Pencegahan, Pengendalian dan Pemberantasan
Infectious Bovine Rhinotracheitis (IBR) ........................... 30
2.4 Bovine Viral Diarrhea (BVD) ....................................................... 32
2.4.1 Etiologi Bovine Viral Diarrhea (BVD) ............................. 32
2.4.2 Epidemiologi Bovine Viral Diarrhea (BVD) ..................... 33
2.4.3 Gejala Klinis Bovine Viral Diarrhea (BVD) ..................... 33
2.4.4 Patogenesa Bovine Viral Diarrhea (BVD)......................... 35
2.4.5 Diagnosa Bovine Viral Diarrhea (BVD) ........................... 36
2.2.5.1 Pengujian Serologis ................................... ............ 36

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 2.2.5.2 Isolasi Virus .................................... ...................... 39
2.4.6 Pencegahan, Pengendalian dan Pemberantasan
Bovine Viral Diarrhea (BVD) ............................................ 41
BAB 3. METODE KEGIATAN .................................................................... 43
3.1 Waktu dan Lokasi Kegiatan ........................................................... 43
3.2 Metode Praktek Kerja Lapang dan Pengambilan Data ................... 43
3.2.1 Pengambilan Sampel .......................................................... 43
3.2.2 Prosedur Pengujian Brucellosis.......................................... 43
3.2.2.1 Uji Aglutinasi Cepat dengan Antigen
RBT (Rose Bengal Test) ........................................ 43
3.2.2.2 Complement Fixation Test (CFT) ......................... 44
3.2.2.3 Complement Fixation Test (CFT) Metode
Maros ................................................................. ... 48
3.2.3 Prosedur Pengujian Infectious Bovine Rhinotracheitis
(IBR) Metode ELISA ........................................................ 52
3.2.4 Prosedur Pengujian Bovine Viral Diarrhea (BVD)
Metode ELISA .................................................................. 55
3.2.5 Wawancara ......................................................................... 58
3.2.6 Studi Pustaka ...................................................................... 58
3.3 Jadwal Kegiatan .............................................................................. 58
3.4 Biodata Peserta Praktek Kerja Lapang ........................................... 59
BAB 4. PELAKSANAAN KEGIATAN ....................................................... 60
4.1 Tempat dan Waktu Pelaksanaan Kegiatan ..................................... 60
4.2 Aktifitas Praktek Keja Lapang........................................................ 60
BAB 5. HASIL DAN PEMBAHASAN ......................................................... 62
5.1 Hasil Pengujian Penyakit Reproduksi pada Ruminansia ................ 62
5.1.1 Pengujian Brucellosis ........................................................... 62
5.1.1.1 Uji Aglutinasi Cepat dengan Antigen RBT ............. 62
5.1.1.2 Complement Fixation Test (CFT) ............................ 67
5.1.1.3 Complement Fixation Test (CFT) Metode Maros ... 70
5.1.2 Pengujian Infectious Bovine Rhinotracheitis (IBR) Metode
ELISA ................................................................................... 73
5.1.3 Pengujian Bovine Viral Diarrhea (BVD) Metode ELISA .. 75
BAB 6. PENUTUP.......................................................................................... 79
6.1 Kesimpulan ....................................................................................... 79
6.2 Saran ................................................................................................. 80
DAFTAR PUSTAKA ..................................................................................... 81
LAMPIRAN .................................................................................................... 86

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