Section A // bahagian A

(answer all the question in section A and B )

(Jawab semua soalan dalam bahagian A dan B)

1 A group of students carried out an experiment to study the effect of enzyme

concentration on the activity of salivary amylase .

Sekumpulan pelajar sedang menjalankan eksperimen untuk mengkaji kesan kepekatan

enzim ke atas aktiviti amilase saliva.

Diagram 1.1 shows the method used by the students. Set the time at one minute
intervals to transfer a drop of mixture from C into groove tile until the yellow color of
iodine solution in the groove remain unchanged. The time taken for the hydrolysis of 1%
starch to be completed is shown in Table 1.

Rajah 1.1 menunjukkan kaedah yang digunakan oleh pelajar. Masa ditetapkan selang
satu minit untuk memindahkan satu titik campuran C ke atas bekas marmar, sehingga
warna kuning iodine kekal tidak berubah. Masa yang diambil untuk melengkapkan
hidrolisis 1% kanji ditunjukkan di dalam Jadual 1.

The whole experiment in Diagram 1.1 was carried out using different concentration of
salivary amylase which is 0.5%, 1.0 % and 1.5%. Table 1.1 shows the results of the
experiment.

Keseluruhan eksperimen pada Rajah 1.1 dijalankan dengan menggunakan kepekaran

amilase saliva yang berbeza iaitu 0.5%, 1.0% dan 1.5%.Rajah 1.1 menunjukkan
keputusan eksperimen tersebut.

Diagram 1.1 / Rajah 1.1

Concentration of salivary
Time taken for hydrolysis of starch to be completed / minutes
amylase (%)
Masa yang diambil untuk mnghidrolisiskan kanji dengan
kepekatan amylase saliva
lengkap /minit
(%)

0.5

1.0

1.5

Table 1 / Jadual 1
a(i) Based on Table 1.1 , state two observations on the relationship between the
quantity of salivary amylase and time.

Berdasarkan Jadual 1.1, nyatakan dua pemerhatian ke atas hubungan antara

kuantiti amylase saliva dengan masa.

Observation 1 / Pemerhatian 1 :

...................................................................................................................................

...................................................................................................................................

Observation 2 / Pemerhatian 2:

...................................................................................................................................

...................................................................................................................................
[3 marks]

(ii) State the inference which corresponds to the observation in 1(a)(i)

Nyatakan inferens yang sepadan dengan pemerhatian di 1(a)(i).
Inference 1 / Inferens 1 :

...................................................................................................................................

...................................................................................................................................

Inference 1 / Inferens 1 :

...................................................................................................................................

...................................................................................................................................
[3 marks]

(b) Complete Table 1 by recording the time taken for the starch solution to hydrolyse
completely.
Lengkapkan Jadual 1 dengan merekodkan masa yang diambil bagi larutan kanji
di hidrolisiskan dengan lengkap.

[3 marks]
(c)(i) Complete Table 1.2 based on this experiment.
Lengkapkan Jadual 1.2 berdasarkan eksperimen ini.

Variable Method to handle the variable

Pembolehubah Cara mengendali pembolehubah

Manipulated variable
Pembolehubah dimanipulasi

---------------------------------
---------------------------------------------------------------------
---------------------------------
---------------------------------------------------------------------
---------------------------------
---------------------------------------------------------------------

Responding variable
Pembolehubah
bergerakbalas

--------------------------------- -------------------------------------------------------------------

--------------------------------- -------------------------------------------------------------------

--------------------------------- -------------------------------------------------------------------

Controlled variable
Pembolehubah kawalan

--------------------------------- -------------------------------------------------------------------

--------------------------------- -------------------------------------------------------------------

--------------------------------- -------------------------------------------------------------------

Table 1.2

[3 marks]

(d) State the hypothesis for this experiment.

Nyatakan hipotesis untuk eksperimen ini.

..................................................................................................................................

..................................................................................................................................

..................................................................................................................................
[3 marks]
e (i) Based on Table 1.1, construct a table and record the results of the experiment
which includes the following aspects:

Berdasarkan Jadual 1.1, binakan satu jadual dan rekodkan keputusan-keputusan

eksperimen yang mana mengandungiaspek berikut:

• Percentage concentration of salivary amylase

Peratus kepekatan amilase saliva

• Time taken for the starch to be hydrolysed completely /min

Masa yang diambil untuk menghidrolisis kanji dengan lengkap /minit

• Rate of enzyme reaction (1/time).

Kadar tindakbalas enzim (1/ minit)
[3 marks]

(ii) On the graph paper, draw the graph of rate of reaction of salivary amylase against
the concentration of salivary amylase.

Pada kertas graf, lukiskan graf kadar tindak balas amilase saliva melawan
kepekatan amilase saliva.

[3 marks]
f) Explain the relationship between the rate of reaction of salivary amylase and the
concentration of salivary amylase based on the graph in1(e)(ii)

Jelaskan hubungan antara kadar tindak balas amilase saliva dan kepekatan
amilase saliva berdasarkan graf dalam 1(e)(ii).

……………………………………………………………………………………………….

……………………………………………………………………………………………….

……………………………………………………………………………………………….

………………………………………………………………………………………………..
[3 marks]

g) Based on this experiment, what can you deduce about rate of salivary amylase
activity?

Berdasarkan eksperimen ini, apa yang anda dapat rumuskan tentangkadar

tindakan amilase liur?

……………………………………………………………………………………………….

……………………………………………………………………………………………….

……………………………………………………………………………………………….

………………………………………………………………………………………………..
[3 marks]

(h) The experiment is repeated using the same apparatus and same materials.
The quantities of salivary amylase used is 1.5% and the temperature used is
20oC. Predict the time taken for the starch to hydrolyse . Explain your prediction.

Eksperimen ini diulang menggunakan radas dan bahan yang sama.

Kuantiti amylase saliva digunakan ialah 1.5% dan suhu digunakan ialah 20oC.
Ramalkan masa diambil untuk kanji dihidrolisiskan. Jelaskan ramalan anda.

……………………………………………………………………………………………….

……………………………………………………………………………………………….

……………………………………………………………………………………………….

………………………………………………………………………………………………..
[3 marks]
(i) The following list is part of the apparatus and material used in this experiment.

Senarai berikut adalah sebahagian dari radas dan bahan yang digunakan di dalam
eksperimen ini

Thermometer Stop watch Salivary amylase

Jangka suhu Jam randik Amilase liur
Starch solution Iodine solution Cavity tile
Larutan kanji Larutan iodin Kepingan marmar

Classify the items into material and apparatus.

Kelaskan item kepada bahan atau radas.

Material Apparatus
Bahan Radas

[3 marks]
Section B

1. Water is very important to plants. It can be lost by evaporation from the plants to the
atmosphere. This is called transpiration. Light intensity is one of the factors that can
affect the rate of transpiration.

Based on the above information, design a laboratory experiment to study the effect of
light intensity on the rate of transpiration in Hibiscus sp.

The planning or your experiment must include the following aspects:

Air adalah sangat penting kepada tumbuhan. Ia boleh hilang melalui sejatan daripada
tumbuhan ke atmosfera. Ini dipanggil sebagai transpirasi. Keamatan cahaya
merupakan satu faktor yang dapat mempengahruhi kadar transpirasi.

Berdasarkan maklumat di atas, reka bentuk satu eksperimen makmal untuk mengkaji
kesan keamatan cahaya ke atas kadar transpirasi dalam Hibiscus sp. (Bunga raya)
Perancangan eksperimen anda hendaklah meliputi aspek-aspek berikut:

 Aim of the exper iment

Objektif eksperim en

 Problem statement
Pernyataan Masalah

 Hypothesis
Hipotesis

 Variables
Pembolehubah

 List of apparatus and materials

Senarai radas dan bahan

 Exper imental procedure

Prosedur eksper imen

 Present ation of data

Persembahan dat a

[17 marks / 17 markah]