Berdasarkan penelitian yang dilakukan oleh Safithri, diperoleh hasil bahwa pemberian isoflavon mampu

mengembalikan fungsi kolinergik di CA1 hipPokampus yang ditandai dengan meningkatnya jumlah sel
pyramidal CA1 yang mengekspresi asetilkolinesterase (Safithri et al., 2010). Hasil ini mendukung penelitian
terdahulu dimana pemberian diet kedelai selama delapan minggu pada tikus paska ooforektomi dapat
meningkatkan aktivitas kolinergik di korteks dan hippocampus yang ditandai dengan peningkatan kadas
ChAT (Pan et al., 1999; Lund et al., 2001; Safithri et al., 2010).

Soy protein supplementation was able to reverse the increase in AChE activity (Monteiro et al., 2007). The
reversal of AChE activity probably indicates that isoflavones can affect brain cholinergic mechanisms, since
stimulation of AChE activity provoked an enhanced acetylcholine (ACh) hydrolysis and choline
reuptake(Okuda et al., 2000; Monteiro et al., 2007). In agreement with our studies, soy isoflavones have been
described to affect the synthesis of acetylcholine. In this context, Pan et al. (1999) reported that soy
phytoestrogens may function as estrogen agonists in regulating choline acetyltransferase and nerve growth
factor in brains of female rats (Pan et al., 1999; Monteiro et al., 2007).

Suplemen protein kedelai mampu membalikkan peningkatan aktivitas AChE (Monteiro et al., 2007).
Pembalikan aktivitas AChE mungkin menunjukkan bahwa isoflavon dapat mempengaruhi mekanisme
kolinergik otak, karena stimulasi aktivitas AChE memicu peningkatan hidrolisis asetilkolin (ACh) dan
pengambilan kembali kolin (Okuda et al., 2000; Monteiro et al., 2007). Dalam perjanjian dengan penelitian
kami, isoflavon kedelai telah dijelaskan mempengaruhi sintesis asetilkolin. Dalam konteks ini, Pan et al.
(1999) melaporkan bahwa fitoestrogen kedelai dapat berfungsi sebagai agonis estrogen dalam mengatur kolin
asetiltransferase dan faktor pertumbuhan saraf pada otak tikus betina (Pan et al., 1999; Monteiro et al., 2007).
 DAFTAR PUSTAKA

Lund, T. D., West, T. W., Tian, L. Y., Bu, L. H., Simmons, D. L., Setchell, K. D. R., Adlercreutz, H. and
Lephart, E. D., 2001. Visual Spatial Memory is Enchanced in Female Rats (But Inhibited in Males) by
Dietary Soy Phytoestrogens. BMC Neuroscience (on-line), 2. Available from: URL:
https://bmcneurosci.biomedcentral.com/track/pdf/10.1186/1471-2202-2-20.
Monteiro, S. C., Mattos, C. B., Scherer, E. B. S. and Wyse, A. T. S., 2007. Supplementation with vitamins E
plus C or soy isoflavones in ovariectomized rats: Effect on the activities of Na+, K+-ATPase and
cholinesterases. Metabolic Brain Disease (on-line), 22: 156–171. Available from: URL:
https://s3.amazonaws.com/academia.edu.documents/48453497/s11011-007-9051-820160830-18114-
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High-Affinity Choline. Nature Neuroscience (on-line), 3: 120–125. Available from: URL:
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Pan, Y., Anthony, M. and Clarkson, T. B., 1999. Effect of Estradiol and Soy Phytoestrogens on Choline
Acetyltransferase and Nerve Growth Factor mRNAs in the Frontal Cortex and Hippocampus of Female
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Safithri, F., Ali, M., Hidayat, A. and Setyawati, S., 2010. Efek Isoflavon Fitoestrogen Dari Ekstrak Pueraria
Lobata Terhadap Memori dan Aktivitas Kolinergil di Hippokampus CA1 Pada Tikus Hipoestrogen.
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