ABSTRAK

Nama

: Novri

Program Studi : Pendidikan Dokter

Judul

: Pengaruh Pemberian Pewarna Sintetis Makanan Sunset Yellow

For Colorong Food (FCF) Peroral Terhadap Kadar Aspartat
Aminotransferase (AST) dan Kadar Alanin Aminotransferase
(ALT) Pada Tikus Putih Jantan (Rattus norvegicus) galur wistar

Pewarna sintetis makanan sangat sering digunakan sebagai bahan tambah

pangan. Sunset Yellow For Coloring Food (FCF) merupakan pewarna dari
golongan azo yang sering digunakan yang dapat menyebabkan kerusakan pada
organ terutama pada hepar. Kerusakan hepar yang terjadi dapat menyebabkan
peningkatan kadar Aspartat Aminotransferase (AST) dan Alanin
Aminotransferase (ALT). Tujuan penelitian ini untuk mengetahui pengaruh
pemberian pewarna sintetis makanan Sunset Yellow FCF peroral terhadap
peningkatan kadar AST dan ALT pada tikus putih jantan (Rattus norvegicus)
galur wistar. Penelitian ini merupakan eksperimental laboratik dengan rancangan
post test control group design menggunakan 24 ekor tikus putih jantan (Rattus
norvegicus) galur wistar terbagi menjadi 4 kelompok dengan 1 kelompok kontrol
yang diberikan aquadest, dan 3 kelompok perlakuan yang diberikan Sunset Yellow
FCF dosis bertingkat berturut-turut 2,5 mg/kbBB/hari, 5 mg/kbBB/hari dan 10
mg/kbBB/hari selama 21 hari. Analisis kadar AST dan ALT menggunakan
hematology autoanalyzer dilakukan pada hari ke 29 dengan pengambilan darah
melalui jantung. Analisis data menggunakan uji One Way ANOVA. Berdasarkan
uji One Way ANOVA terdapat perbedaan yang nyata kadar AST (p=0,003) dan
kadar ALT (p=0,000) di antara kelompok kontrol dengan kelompok perlakuan.
Kesimpulan penelitian ini menunjukan pemberian pewarna sintetis makanan
Sunset Yellow FCF peroral berpengaruh terhadap peningkatan kadar AST dan
ALT pada tikus putih jantan (Rattus norvegicus) galur wistar.
Kata Kunci :
Kadar Aspartat Aminotransferase (AST), Kadar Alanin Aminotransferase
(ALT), Sunset Yellow FCF, Tikus Putih (Rattus norvegicus) Jantan Galur
Wistar

ABSTRACT

Name

: Novri

Programe

: Medicine

Title

: The Effect of Synthetic Food Dye Sunset Yellow For Coloring

Food (FCF) That Given Orally to The Level of Aspartate
Aminotransferase (AST) and Alanine Aminotransferase (ALT)
Levels in Male Rats (Rattus norvegicus) Wistar Strain

Synthetic food dyes were commonly used as a food additive. Sunset

Yellow Coloring For Food (FCF) is an azo group dye that commonly used that
possibly can cause damage to the organs especially in liver. The liver damaged
can increase the level of Aspartat Aminotransferase (AST) dan Alanin
Aminotransferase (ALT). This study aimed to determine the effect of synthetic
food dye Sunset Yellow FCF that given orally to the increase of Aspartate
Aminotransferase (AST) and Alanine Aminotransferase (ALT) levels in male rats
(Rattus norvegicus) Wistar strain.This was an experimental laboratic study with
post test control group design using 24 male rats (Rattus norvegicus) Wistar strain
that divided into four groups with one control group given distilled water, and 3
treatment groups that given Sunset Yellow FCF with gradually doses 2.5 mg/kg of
body weight/day, 5 mg/kg of body weight/day and 10 mg/kg of body weight/day
in 21 days treatment. The analysis of AST and ALT levels using hematology
autoanalyzer at after 29 days with the blood that taken from the heart. The data
analyzed with One Way ANOVA test. The result showed that there was a
significant different of AST level (p=0,003) and ALT level (p=0,000) between
control group and treatment groups. The conclusion of this study showed that
there was the effect of synthetic food dye Sunset Yellow FCF that given orally to
the increase of AST and ALT levels in male rats (Rattus norvegicus) wistar strain.
Keywords :
Level of Aspartat Aminotransferase (AST), Level of Alanin Aminotransferase
(ALT), Sunset Yellow FCF,

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