PERBEDAAN KADAR TESTOSTERON SERUM TIKUS PUTIH (Rattus

norvegicus) JANTAN PASCA INDUKSI BERBAGAI MODEL STRES

SLEEP DEPRIVATION

Safira Aulia1, Fitranto Arjadi2, Vitasari Indriani3

Fakultas Kedokteran, Universitas Jenderal Soedirman, Purwokerto,
Jawa Tengah, Indonesia
E-mail: safiraku8@gmail.com

ABSTRAK
Latar belakang : Paradoxical sleep deprivation (PSD) dan total sleep deprivation
(TSD) dapat menyebabkan gangguan aksis HPA, aksis HPG, dan peningkatan stres
oksidatif yang berhubungan dengan masalah infertilitas pria. Sleep recovery (SR)
dapat menginduksi pengeluaran hormon melatonin sebagai antioksidan untuk
memperbaiki fungsi reproduksi pria.
Tujuan : Mengetahui perbedaan kadar testosteron serum pada tikus putih (Rattus
norvegicus) jantan pasca induksi berbagai model stres sleep deprivation
Metode : Penelitian ini merupakan penelitian eksperimental dengan desain posttest
only with control group. Tiga puluh ekor tikus putih dibagi secara acak menjadi 5
kelompok yaitu A (kontrol sehat), B (PSD 20 jam/hari selama 5 hari), C (TSD
24jam/hari selama 5 hari), D (PSD 20 jam/hari selama 5 hari pertama dilanjutkan
dengan sleep recovery selama 5 hari berikutnya), dan E (TSD selama 24 jam/hari
selama 5 hari pertama dilanjutkan dengan sleep recovery selama 5 hari berikutnya).
Hasil : Rerata kadar Testosteron tertinggi terdapat pada kelompok B (19,79±9,22
ng/ml), diikuti kelompok D (14,78±9,78 ng/ml), kelompok A (13,68±6,02 ng/ml),
kelompok E (10,48±4,83 ng/ml), dan terendah kelompok C (10,30±5,65 ng/ml). Uji
One Way ANOVA menunjukkan tidak terdapat perbedaan signifikan (p>0,05)
rerata kadar tetsosteron pada semua kelompok penelitian.
Kesimpulan : Tidak terdapat perbedaan signifikan rerata kadar Testosteron Serum
pada tikus putih (Rattus norvegicus) jantan pasca induksi berbagai model stres sleep
deprivation.

Kata kunci : Paradoxical Sleep Deprivation (PSD), Total Sleep Deprivation (TSD),
Sleep Recovery, Testosteron Serum (T)
 THE DIFFERENCE OF TESTOSTERON SERUM LEVEL IN MALE
ALBINO RATS (Rattus norvegicus) AFTER INDUCTION VARIOUS
MODELS OF SLEEP DEPRIVATION STRESS

Safira Aulia1, Fitranto Arjadi2, Vitasari Indriani3

Medical Faculty, Jenderal Soedirman University, Purwokerto,
Jawa Tengah, Indonesia
E-mail: safiraku8@gmail.com

ABSTRACT
Background : Sleep Deprivation can cause disrupt of HPA axis, HPG axis, and
increased oxidative stress which related with male infertility. Sleep recovery (SR)
can induce secretion of melatonin as an antioxidant to improve male reproduction
function.
Objective : To know the difference of Testosteron Serum level in male albino rats
(Rattus norvegicus) after induction various models of sleep deprivation stress.
Method: This research was an experimental research with posttest only and control
group design. Thirty male albino rats were distributed into 5 groups, A (health
control), B (PSD 20 hours/day for 5 days), C (TSD 24 hours/day for 5 days),
D (PSD 20 hours/day for the first 5 days then continue with sleep recovery for the
next 5 days), and E (TSD 24 hours/day for the first 5 days then continue with sleep
recovery for the next 5 days).
Result: The highest mean of Testosteron found in group B (19,79±9,22 ng/ml),
followed by group D (14,78±9,78 ng/ml), group A (13,68±6,02 ng/ml), group E
(10,48±4,83 ng/ml), while the lowest mean found in group C (10,30±5,65 ng/ml).
One Way ANOVA test showed no significant difference (p> 0,05) of Testosteron
mean level in all study groups.
Conclusion : There was no significant difference of Testosteron Serum mean level
in male albino rats (Rattus norvegicus) after the induction various models of sleep
deprivation stress.

Keyword : Paradoxical Sleep Deprivation (PSD), Total Sleep Deprivation (TSD),

Sleep Recovery, Testosteron Serum (T)