ABSTRAK

Evaluasi Arsitektur Dan Permeabilitas

Febri Ramdani
Judul Scaffold Fibroin Sutra Hasil Fabrikasi Direct
Nugraha
Dissolution Salt Leaching
Program Studi Teknik Material 13713501
Fakultas Teknik Mesin dan Dirgantara
Institut Teknologi Bnadung
Scaffold dalam tissue engineering digunakan sebagai template untuk
memfasilitasi dan menyokong penempelan, pertumbuhan, ploliferasi dan
diferensiasi sel atau jaringan. Fabrikasi scaffold fibroin sutra dengan metode
direct dissolution salt leaching telah berhasil dikembangkan untuk membuat
scaffold yang biokompatibel dan biodegradabel untuk menyokong pertumbuhan
sel, serta secara signifikan mengurangi waktu dan temperatur pemrosesan
dibandingkan metode konvensional. Arsitektur scaffold memiliki dampak
langsung pada penempelan, pertumbuhan, dan migrasi sel melalui scaffold.
Permeabilitas scaffold mempengaruhi difusi nutrisi dan oksigen, pembuangan
limbah, dan migrasi sel ke dalam scaffold. Pada penelitian ini dilakukan evaluasi
arsitektur pori serta permeabilitas terhadap scaffold hasil fabrikasi direct
dissolution salt leaching. Arsitektur scaffold yang dievaluasi adalah ukuran pori,
porositas scaffold, ketebalan dinding scaffold, interkonektivitas pori, dan luas
permukaan spesifik scaffold. Parameter proses yang divariasikan adalah ukuran
partikel NaCl (158±47 μm, 250±58 μm, 378±42 μm, 503±31 μm) dan konsentrasi
fibroin sutra (6% w/v, 8% w/v, 10% w/v, 12% w/v). Evaluasi arsitektur scaffold
dilakukan melalui metode microcomputed tomography (micro-CT).
Permeabilitas scaffold diukur berdasarkan metode falling head. Evaluasi 3D dari
micro-CT dapat digunakan untuk melakukan evaluasi ukuran pori, porositas
scaffold, ketebalan dinding scaffold, interkonektivitas pori dan luas permukaan
spesifik scaffold. Kenaikan ukuran partikel NaCl menghasilkan peningkatan
ukuran pori, porositas scaffold, ketebalan dinding scaffold, interkonektivitas pori,
permeabilitas spesifik scaffold serta penurunan luas permukaan spesifik scaffold.
Peningkatan konsentrasi fibroin sutra tidak menghasilkan perubahan arsitektur
dan permeabilitas scaffold yang signifikan. Semakin besar ukuran pori, maka
porositas scaffold, ketebalan dinding scaffold, interkonektivitas pori semakin
tinggi, namun luas permukaan spesifik scaffold semakin rendah. Porositas
scaffold, dan interkonektivitas pori scaffold yang semakin tinggi, serta luas
permukaan spesifik scaffold yang semakin rendah, menghasilkan permeabilitas
spesifik scaffold yang semakin tinggi.

Kata kunci: pertumbuhan sel, direct dissolution, arsitektur, micro-CT,

permeabilitas
 ABSTRACT

Architecture And Permeability Evaluation

Febri Ramdani
Tittle Of Silk Fibroin Scaffold from Direct
Nugraha
Dissolution Salt Leaching Method
Major Teknik Material 13713501
Faculty of Mechanical and Aerospace Engineering
Bandung Institute of Technology
Tissue engineering scaffold is used as a template to facilitate and support
the attachment, growth, proliferation, and differentiation of cells or tissues. The
fabricating of silk fibroin scaffold with the direct dissolution salt leaching method
has been successfully developed to produce biocompatible and biodegradable
scaffolds that support cell growth, and significantly reduce processing time and
temperature compared to conventional methods. The scaffold architecture has a
direct impact on attachment, growth, and migration of cells through the scaffold.
Permeability affects the diffusion of nutrients, oxygen, waste, and cell migration
into the scaffold. In this study evaluation of pore architecture and permeability
of scaffolds, which is the result direct dissolution salt leaching method. The
evaluated scaffold architecture are pore size, scaffold porosity, wall thickness
scaffold, pore interconnectivity, and the scaffold specific surface area. The varied
process parameters are NaCl particle size (158 ± 47 μm, 250 ± 58 μm, 378 ± 42
μm, 503 ± 31 μm), and silk fibroin concentration (6% w / v, 8% w / v, 10% w / v,
12% w / v). The evaluation of scaffold architecture using microcomputed
tomography (micro-CT) method. The scaffold permeability measured using
falling head method. The 3D evaluation of micro-CT can be used to evaluate pore
size, scaffold porosity, wall thickness scaffold, pore interconnectivity, and the
scaffold specific surface area. The increasing of NaCl particle size generates
increasing of pore size, scaffold porosity, scaffold wall thickness, pore
interconnectivity, and the scaffold specific permeability, while the scaffold
specific surface area was decrease. The increasing of silk fibroin concentration
doesn’t result in significant changes in scaffold architecture and scaffold
permeability. The larger of pore size, the larger of scaffold porosity, wall
thickness scaffold, pore interconnectivity was result, but the smaller of scaffold
spesific surface area. The higher of the scaffold porosity, and the scaffold pore
interceptivity, while the lower scaffold specific surface area, results in higher
specific scaffold permeability.

Keyword: cell growth, direct dissolution, architectures, micro-CT,

permeability