PROPOSAL PENELITIAN

MODIFIKASI AIR PERASAN JERUK NIPIS (CITRUS AURANTIOLA)

SEBAGAI PENGGANTI LARUTAN TURK DALAM HITUNG JUMLAH
SEL LEUKOSIT METODE MANUAL

AGIL SAPUTRA
PO.71.4.203.17.2.002

KEMENTERIAN KESEHATAN REPUBLIK INDONESIA

POLITEKNIK KESEHATAN KEMENKES MAKASSAR
JURUSAN ANALIS KESEHATAN
PRODI DIV ANALIS KESEHATAN
2018

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 DAFTAR ISI

Sampul Dalam .................................................................................. i

Daftar Isi ........................................................................................... ii

Daftar Gambar .................................................................................. v

BAB I PENDAHULUAN .................................................................... 1

A. Latar belakang ..................................................................... 1

B. Rumusan masalah ............................................................... 3

C. Tujuan penelitian ................................................................. 3

1. Umum ....................................................................... 3

2. Khusus ..................................................................... 3

D. Manfaat penelitian ............................................................... 4

1. Akademik .................................................................. 4

2. Peneliti ..................................................................... 4

3. Petugas laboratorium ................................................ 4

BAB II TINJAUAN PUSTAKA ........................................................... 5

A. Tinjauan tentang darah ....................................................... 5

1. Pengertian darah ...................................................... 5

2. Komposisi darah........................................................ 5

a) Plasma darah ................................................. 5

b) Sel sel darah .................................................. 6

1. Sel darah merah .................................. 6

2. Sel darah putih ..................................... 9

3. Trombosit ............................................ 12

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 3. Pembentukan darah (Hematopoesis) ........................ 14

4. Fungsi darah ............................................................ 17

B. Tinjauan tentang jeruk nipis .................................. ............. . 18

1. Taksonomi ................................................................ 18

2. Morfologi.................................................................... 19

3. Kandungan dan kegunaan ....................................... 19

C. Pemeriksaan jumah leukosit ................................. .............. 20

D. Kamar hitung improved neubauer ......................... .............. 22

E. Antikoagulan ....................................................................... 23

F. Kerangka pikir ...................................................................... 26

G. Hipotesis ............................................................................. 27

BAB III METODE PENELITIAN ........................................................ 29

A. Jenis penelitian ...................................................................... 29

B. Sampel ,besar sampel dan teknik pengambilan sampel ......... 29

1. Sampel ........................................................................ 29

2. Besar sampel................................................................ 29

3. Teknik pengambilan sampel ......................................... 29

C. Kerangka operasional ............................................................ 30

D. Variabel penelitian .................................................................. 31

1. Variabel bebas.............................................................. 31

2. Variabel terikat.............................................................. 31

E. Definisi operasional ................................................................ 31

F. Lokasi dan waktu penelitian .................................................... 31

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 1. Lokasi .......................................................................... 32

2. Waktu penelitian ........................................................... 32

G. Penggumpulan data ............................................................... 32

1. Spesimen .................................................................... 32

2. Alat dan bahan ............................................................ 32

a) Alat ................................................................... 32

b) Bahan ............................................................... 33

3. Prosedur pemeriksaan ................................................ 33

a) Pengambilan darah vena ................................... 33

b) Pengenceran air perasan jeruk nipis ................. 34

c) Pemeriksaan hitung jumlah leukosit ................. 36

H. Analisa Data ............................................................................ 38

DAFTAR PUSTAKA

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 DAFTAR GAMBAR

Gambar 2.1 Jeruk Nipis ................................................................... 18

Gambar 2.2 Kamar Hitung Improved Neubauer ................................ 22
Gambar 2.3 Kerangka Pikir ................................................................ 27
Gambar 3.1 Skema Kerangka Operasional ...................................... 30