HASIL PENGAMATAN

ACARA I

UJI SANITASI PEKERJA PENGOLAHAN PANGAN

Hasil Pengamatan
Tabel 1.1 Hasil Pengamatan Uji Kebersihan Tangan
Media
Eosin
Plate Count Methylene
Klp Perlakuan  Koloni  Koloni
Agar (PCA) Blue
(CFU) (CFU)
(EMBA)
U1 U2 U1 U2
Tanpa cuci
6 35 >250 >250 0 0 0
tangan
Dicuci air
7 >250 105 >250 0 0 0
mengalir
Dicuci air
8 25 20 22,5 0 0 0
tergenang
Dicuci air
9 79 81 80 0 0 0
dipakai berulang
10 Air hangat 24 43 33,5 0 0 0

Tabel 1.2 Hasil Pengamatan Uji Daya Antiseptik Sabun

Media
Eosin
Plate Count Methylene
Klp Perlakuan  Koloni  Koloni
Agar (PCA) Blue Agar
(CFU) (CFU)
(EMBA)
U1 U2 U1 U2
6 Alkohol 70% >250 10 >250 0 0 0
LUX (sabun
7 228 130 179 0 0 0
kecantikkan)
8 Antis gel 17 12 14,5 0 0 0
9 Dettol 27 48 37,5 0 0 0
 10 Handsoop 96 47 71,5 0 0 0

Tabel 1.3 Hasil Pengamatan Uji Kontaminan Sabun

Media
Potato
Nutrient
Klp Perlakuan Dextrose Agar
 Koloni Agar (NA)  Koloni
(PDA)
(CFU) (CFU)
U1 U2 U1 U2
6 Terbuka 3 5 4 169 13 91
Tertutup 6 2 4 15 22 18,5
7 Terbuka 0 0 0 3 16 9,5
Tertutup 8 6 7 8 12 10
8 Terbuka 3 0 1,5 8 26 17
Tertutup 8 8 8 10 21 15,5
9 Terbuka 1 1 1 49 44 46,5
Tertutup 0 21 10,5 24 37 30,5
10 Terbuka 1 18 9,5 51 48 49,5
Tertutup 19 10 14,5 16 10 13

Hasil Perhitungan
1. Hasil Perhitungan Uji Kebersihan Tangan
a. Kelompok 6 (tanpa cicu tangan )
- Media Plate Count Agar (PCA)
U 1+U 2
 Koloni =
2
35+¿ 250
=
2
= >250 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU
b. Kelompok 7 (dicuci air mengalir)
- Media Plate Count Agar (PCA)
 U 1+U 2
 Koloni =
2
¿ 250+105
=
2
= >250 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU
c. Kelompok 8 (dicuci air tergenang)
- Media Plate Count Agar (PCA)
U 1+U 2
 Koloni =
2
25+20
=
2
= 22,5 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU
d. Kelompok 9 (dicuci pakai air berulang)
- Media Plate Count Agar (PCA)
U 1+U 2
 Koloni =
2
79+81
=
2
= 80 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
 0+0
=
2
= 0 CFU
e. Kelompok 10 (air hangat)
- Media Plate Count Agar (PCA)
U 1+U 2
 Koloni =
2
24+ 43
=
2
= 33,5 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU

2. Hasil Perhitungan Uji Daya Antiseptik

a. Kelompok 6 (alkohol 70%)
- Media Plate Count Agar (PCA)
U 1+U 2
 Koloni =
2
¿ 250+0
=
2
= >250 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU
b. Kelompok 7 (LUX (sabun kecantikkan)
- Media Plate Count Agar (PCA)
 U 1+U 2
 Koloni =
2
228+130
=
2
= 179 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU
c. Kelompok 8 (antis gel)
- Media Plate Count Agar (PCA)
U 1+U 2
 Koloni =
2
17+12
=
2
= 14,5 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU
d. Kelompok 9 (dettol)
- Media Plate Count Agar (PCA)
U 1+U 2
 Koloni =
2
27+48
=
2
= 37,5 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
 0+0
=
2
= 0 CFU
e. Kelompok 10 (Handshop)
- Media Plate Count Agar (PCA)
U 1+U 2
 Koloni =
2
96+ 47
=
2
= 71,5 CFU
- Media Eosin Methylene Agar (EMBA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU

3. Hasil Perhitungaan Uji Kontaminasi dari Rambut

a. Kelmpok 6
 Terbuka
- Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
3+5
=
2
= 4 CFU
- Media Nutrient Agar (NA)
U 1+U 2
 Koloni =
2
169+ 13
=
2
= 91 CFU
 Tertutup
 - Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
6+2
=
2
= 4 CFU
- Media Nutrient Agar (NA)
U 1+U 2
 Koloni =
2
15+22
=
2
= 18,5 CFU
b. Kelompok 7
 Terbuka
- Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
0+0
=
2
= 0 CFU
- Media Nutrient Agar (NA)
U 1+U 2
 Koloni =
2
3+16
=
2
= 9,5 CFU
 Tertutup
- Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
8+6
=
2
= 7 CFU
- Media Nutrient Agar (NA)
 U 1+U 2
 Koloni =
2
8+12
=
2
= 10 CFU
c. Kelompok 8
 Terbuka
- Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
3+0
=
2
= 1,5 CFU
- Media Nutrient Agar (NA)
U 1+U 2
 Koloni =
2
8+26
=
2
= 17 CFU
 Tertutup
- Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
8+8
=
2
= 8 CFU
- Media Nutrient Agar (NA)
U 1+U 2
 Koloni =
2
10+21
=
2
= 15,5 CFU
d. Kelompok 9
 Terbuka
 - Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
1+ 1
=
2
= 1 CFU
- Media Nutrient Agar (NA)
U 1+U 2
 Koloni =
2
49+ 44
=
2
= 46,5 CFU
 Tertutup
- Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
0+21
=
2
= 10,5 CFU
- Media Nutrient Agar (NA)
U 1+U 2
 Koloni =
2
24+37
=
2
= 30,5 CFU
e. Kelompok 10
 Terbuka
- Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
1+ 18
=
2
= 9,5 CFU
- Media Nutrient Agar (NA)
 U 1+U 2
 Koloni =
2
51+ 48
=
2
= 49,5 CFU
 Tertutup
- Media Potato Dextrose Agar (PDA)
U 1+U 2
 Koloni =
2
19+10
=
2
= 14,5 CFU
- Media Nutrient Agar (NA)
U 1+U 2
 Koloni =
2
16+10
=
2
= 13 CFU

Mataram, 31 Maret 2020

Mengetahi,
Co. Asst Praktikum Sanitasi Industri Pangan Praktikan

Istiarini Melinda Ade Kantari

NIM : J1A017047 NIM : J1A018074