RIBOSOM & SINTESIS

PROTEIN
 KOMPONEN SINTESIS PROTEIN

 DNA
 mRNA
 tRNA
 Ribosom
 Asam amino
 Retikulum
Endoplasma
Kasar
 Polimerase
 RIBOSOM

 Mpk t4 sel membuat protein

 Dihasilkan nukleolus
 Sel yg laju sintesis protein tinggi mpy ribosom yg
sangat banyak  nukleoli terlihat lebih jelas
 Bebas  tersuspensi di sitosol, menghasilkan
protein yg bekerja di sitosol  enzim2 metabolisme
 Terikat  melekat pd retikulum endoplasma kasar,
menghasilkan protein yg disekresi
 Keduanya identik & dpt saling bertukar t4
SINTESIS RIBOSOM
 RIBOSOM

 T.a subunit kecil & subunit besar

 Subunit tss protein & rRNA (ribosomal RNA)
 POLIRIBOSOM

 Beberapa ribosom melakukan translasi secara

berurutan dalam satu mRNA
SINTESIS
PROTEIN
ILUSTRASI TERJADINYA TRANSKRIPSI
proses transkripsi yang terlihat dengan
menggunakan mikroskop
 mRNA PROCESSING

 Hasil proses transkripsi sebuah gen yang

mengkode protein adalah sebuah pre-
mRNA (hnRNA/mRNA precursor)
 Pre-mRNA (pada eukariotik) terdiri dari
intron dan exon
Proses pematangan mRNA dengan membuang
bagian intron
 2nd base
U C A G
(Phe/F)Phenylalan
UUU UCU (Ser/S) Serine UAU (Tyr/Y)Tyrosine UGU (Cys/C)Cysteine
ine
(Phe/F)
UUC UCC (Ser/S) Serine UAC (Tyr/Y) Tyrosine UGC (Cys/C) Cysteine
U Phenylalanine
UUA (Leu/L) Leucine UCA (Ser/S) Serine UAA Stop (Ochre) UGA Stop (Opal)
(Trp/W)Tryptopha
UUG (Leu/L) Leucine UCG (Ser/S) Serine UAG Stop (Amber) UGG
n
CUU (Leu/L) Leucine CCU (Pro/P) Proline CAU (His/H)Histidine CGU (Arg/R)Arginine
CUC (Leu/L) Leucine CCC (Pro/P) Proline CAC (His/H) Histidine CGC (Arg/R) Arginine
C
CUA (Leu/L) Leucine CCA (Pro/P) Proline CAA (Gln/Q)Glutamine CGA (Arg/R) Arginine
CUG (Leu/L) Leucine CCG (Pro/P) Proline CAG (Gln/Q) Glutamine CGG (Arg/R) Arginine
1st
(Thr/T)Threonine
base AUU (Ile/I) Isoleucine ACU AAU (Asn/N)Asparagine AGU (Ser/S) Serine

AUC (Ile/I) Isoleucine ACC (Thr/T) Threonine AAC (Asn/N) Asparagine AGC (Ser/S) Serine
A
AUA (Ile/I) Isoleucine ACA (Thr/T) Threonine AAA (Lys/K) Lysine AGA (Arg/R) Arginine
AUG[ (Met/M)Methionin
A] ACG (Thr/T) Threonine AAG (Lys/K) Lysine AGG (Arg/R) Arginine
e
GUU (Val/V) Valine GCU (Ala/A) Alanine GAU (Asp/D)Aspartic acid GGU (Gly/G)Glycine
(Asp/D) Aspartic
GUC (Val/V) Valine GCC (Ala/A) Alanine GAC GGC (Gly/G) Glycine
acid
G
GUA (Val/V) Valine GCA (Ala/A) Alanine GAA (Glu/E)Glutamic acid GGA (Gly/G) Glycine
(Glu/E) Glutamic
GUG (Val/V) Valine GCG (Ala/A) Alanine GAG GGG (Gly/G) Glycine
acid
Ringkasan sintesis protein pada prokariot dan eukariot
The main difference between protein synthesis in
eukaryotes and in prokaryotes is:

1. where the process happens

2. what the process produces
3. the participants in the process