Analisis Asam Amino dan Mineral (Kalsium, Magnesium, dan Besi) pada Ikan

Baronang (Siganus guttatus) Asal Pulau Osi Kabupaten Seram Bagian Barat

ISNA FEBRYANI MANDAYA

NIM. 2016-78-005

Abstrak

Telah dilakukan penelitian analisis asam amino dan mineral (Kalsium, Magnesium,
dan Besi) pada Ikan Baronang (Siganus guttatus) Asal Pulau Osi Kabupaten Seram
Bagian Barat. Identifikasi komponen asam amino menggunakan UPLC. Hidrolisis
asam amino dilakukkan secara asam menggunakan H2SO4 pekat 6 M dan hidrolisis
secara basa menggunakan Ba(OH) 6 M. Hasil analisis diperoleh kandungan asam
amino pada sampel ikan baronang yang dihidrolisis secara asam diperoleh 15 jenis
asam amino yaitu: serin (307,29 mg/kg), asam glutamat (2163,10 mg/kg), fenilalanin
(881,01 mg/kg), isoleusin (1119,05), valin (1181,21 mg/kg), alanin (1220,18 mg/kg),
arginin (967,49 mg/kg), glisin (708,96 mg/kg), lisin (1543,21 mg/kg) asam aspartat
(520,47 mg/kg), leusin (1682,54 mg/kg), tirosin (216,07 mg/kg) prolin (704,81
mg/kg), treonin (678,66 mg/kg), dan histidin (455,76 mg/kg). Ikan Baronang yang
dihidrolisis secara basa diperoleh 14 jenis asam amino yaitu: asam glutamat (444,61
mg/kg), fenilalanin (430,80 mg/kg), isoleusin (464,95 mg/kg) valin (525,27 mg/kg),
alanin (734,00 mg/kg), glisin (428,40 mg/kg), lisin (384,77 mg/kg), asam aspartat
(107,91 mg/kg) leusin (853,50 mg/kg) tirosin (327,03 mg/kg), prolin (364,07 mg/kg),
histidin (39,41 mg/kg), metionin (38,17 mg/kg), dan triptofan (63,73 mg/kg). Hasil
analisis kandungan mineral menggunakan spektrofotometer serapan atom (SSA)
menunjukkan bahwa sampel ikan baronang memiliki kandungan Kalsium (Ca)
sebanyak 232.185,09 mg/kg, Magnesium (Mg) sebesar 153.176,66 mg/kg dan Besi
(Fe) 1.507,09 mg/kg.

Kata Kunci : Asam amino, besi, ikan baronang, kalsium, magnesium, SSA, UPLC.

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 Analysis of Amino Acids and Minerals (Calcium, Magnesium, and Iron) in
Baronang Fish (Siganus guttatus) from Osi Island, West Regency

ISNA FEBRYANI MANDAYA

NIM. 2016-78-005

Abstract

Amino acid and mineral analysis research has been carried out (Calcium,
Magnesium, and Iron) on Baronang Fish (Siganus guttatus) from Osi Island, West
Seram Regency. Identification of amino acid components using UPLC.Hydrolysis
of amino acid as acid used 6 M H2SO4 and 6 M Ba(OH) as hydrolysis base. The
analysis showed that the content in the baronang fish sample as acid yield 15
kinds of amino acid: serine (307.29 mg). /kg), glutamic acid (2163.10 mg/kg),
phenylalanine (881.01 mg/kg), isoleucine (1119.05), valine (1181.21 mg/kg),
alanine (1220.18 mg/kg) ), arginine (967.49 mg/kg), glycine (708.96 mg/kg),
lysine (1543.21 mg/kg) aspartic acid (520.47 mg/kg), leucine (1682.54 mg/kg ),
tyrosine (216.07 mg/kg), proline (704.81 mg/kg), threonine (678.66 mg/kg), and
histidine (455.76 mg/kg). Hydrolysis of Baronang Fish as base yield 14 kinds of
amino acid: glutamic acid (444.61 mg/kg), phenylalanine (430.80 mg/kg),
isoleucine (464.95 mg/kg) valine (525.27 mg/kg). kg), alanine (734.00 mg/kg),
glycine (428.40 mg/kg), lysine (384.77 mg/kg), aspartic acid (107.91 mg/kg)
leucine (853.50 mg/kg), kg) tyrosine (327.03 mg/kg), proline (364.07 mg/kg),
histidine (39.41 mg/kg), methionine (38.17 mg/kg), and tryptophan (63.73
mg/kg). kg). The of the mineral content analysis using atomic absorption
spectrophotometer (SSA) showed that the baronang fish sample contained
Calcium (Ca) of 232,185.09 mg/kg, Magnesium (Mg) of 153,176.66 mg/kg and
Iron (Fe) 1,507.09 mg /kg.

Keywords : Amino acids, baronang fish, calcium, iron, magnesium, SSA, UPLC

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