Abiotik
Biotik (Fisikokimia)
UV
UV Nampak
Nampak Ionisasi
Ionisasi
PENGENDALI LINGKUNGAN ABIOTIK
Potensi
Keturunan Daya &
Kualitas
+ Metabolisme
Pertumb.
& Hasil
Lingkungan Tan.P
Abiotik
Biotik
Abiotik
Biotik (Fisikokimia)
UV
UV Nampak
Nampak Ionisasi
Ionisasi
Respon Tanaman Terhadap Cahaya/Radiasi
V B G Y O R
UV IR
< 400 400-425 425-490 490-580 580-585 585-640 640-740 >740
Cahaya nampak
ITMT/S1/SAN
Respon Tanaman Terhadap Cahaya/Radiasi
B. Mekanisme toleransi/adaptasi tanaman terhadap
cahaya kurang/shade/naungan (shade plants):
Efisiensi
Metabolisme
ITMT/S1/SAN
Respon Tanaman Terhadap Cahaya/Radiasi
C. Mekanisme toleransi/adaptasi tanaman terhadap
cahaya lebih/bright (sun plants):
RuBP Fotosintesis
Carboxilase & TKC Khlorofil
Lignifikasi
terlindungi
dari
kerusakan
Fotoinhibitor Suberin, Wax,
Kutikula,
Dinding Sel
ITMT/S1/SAN
Respon Tanaman Terhadap Cahaya/Radiasi
D. Respon Phytohormone Terhadap Cahaya
R Phytohormone : molekul
UV IR
(prot.+chromophore)
yang sensitif thd cahaya
Light / R R-FR (Light-Dark)
Pr Pfr
Dark / FR Pr Pfr
0-15’ = mempengaruhi
permeabilitas
Light/ Phytohormone membran
Dark 1-3 jam = Ekspresi gen
(IAA, GA, ETH,
- IAA & GA - ETH & ABA dan ABA)
R
- Phenolic > 12 jam= Fotomorfogenik
Fotomorfogenik
1. Leaf Expansion, Seed Germination, Stem Elongation, Unrooling Leaf ~ GA
2. Root Growth, Translocation, Fototropism ~ IAA/Auksin
ITMT/S1/SAN
Respon Tanaman Terhadap Cahaya/Radiasi
E. Variabel Pengamatan Respon Tanaman thd
Cahaya
Morfologi : Anatomi daun (ketebalan dan luasan) +
Karakter pertumbuhan tanaman
Psikrofil
Psikrofil Mesofil
Mesofil Termofil
Termofil
Tan. yg senang Tan. yg senang Tan. yg senang
tdingin (0-20)oC tsedang (10-30)oC tpanas (>30)oC
?? Batas Stres
LD50% = 50%
P P P P
LD50%
t t t t
Plant Response to Temperature Stress
A. Sequence of Event in Injury During Stress
Change in
Membrane Mitochondria and Lack of
Breakdown Chloroplast Oxygen
- Necrosis - Reduced Growth - Failure of Seed
- Browning - Discoloration - Chemical
- Tissue Break - Toxin &
Prot.breakdown Decomposition
DEATH
DEATH
ITMT/S1/SAN
Plant Response to Temperature Stress
B. Mechanisms of Plant Tolerance to High Temperature
Loss of Lipid
Respiration Photosynthesis Protein
& TCP Breakdown
Lipid PER
ADP/O2
Increase
Root Growth
Saturated of Protectant
Lipid
Absorption
ITMT/S1/SAN
Plant Response to Temperature Stress
C. Mechanisms of Plant Tolerance to Low Temperature
Loss of Lipid
Respiration Photosynthesis Protein
& TCP Breakdown
Lipid PER
ADP/O2
Increase
Root Growth
Unsaturated of Protectant
Lipid
Absorption
ITMT/S1/SAN
Plant Response to Temperature Stress
D. Mechanisms of Phytohormone toTemperature Stress
0-15’ = mempengaruhi
permeabilitas
Temperature Phytohormone membran
1-3 jam = Ekspresi gen
(GA, CK, ETH,
dan ABA)
> 12 jam= morfogenik
Low ABA+ETH ; GA+CK
Fotomorfogenik
ITMT/S1/SAN
Plant Response to Temperature Stress
E. Variabel of Plant Response to Temperature
Morphology : Leaf anatomy (necrosis, discoloration,
browning, tissue breakdown), root growth,
and component of yield.
Tolerance :
Selection of Some Forage Crop to Light and
Temperature Stress
ITMT/S1/SAN
Respon Tanaman Terhadap
Cekaman Air
A. Cekaman Air : Aspek Fisik dan Kimia
FUNGSI AIR :
1. Bagian esensial protoplasma, 75-90% bobot segar jaringan.
2. Pelarut (gas dan garam)
3. Pereaksi dalam reaksi fotosintesis dan proses hidrolisis
4. Penjaga turgiditas dalam : pembesaran sel, membuka dan
menutupnya stomata, penyangga bentuk/morfologi daun
dan struktur jaringan yang berlignin.
?? Batas Stres/Cekaman
LD50% = 50%
Kekurangan
Kekurangan Kelebihan
Kelebihan
Cekaman Air
Kekurangan
Kekurangan Kelebihan
Insufficient
Toxin NA &
Growth
Inhibition Hormone
Protein
Breakdown
DEATH
DEATH
ITMT/S1/SAN
Plant Response to Water Stress (Flood)
B. Sequence of Event in Injury During Stress
O2 Deficit
Leaching
CO2 Excess
ETH Excess
Metabolic
Toxins
Nutrient Ion
Deficit
DEATH
DEATH
ITMT/S1/SAN
Plant Response to Water Stress
C. Mechanisms of Plant Tolerance to Water Stress/Drought
Dehydration Loss of RNA
Protein RNAse
Loss of Decrease
Conformation
Turgor Protein
Synthesis
Loss of Accumulation of AA
Enzyme
Pigment Methionine Osmoregulation
Breakdown
N Metabolism
0-15’ = mempengaruhi
permeabilitas
Water Stress Phytohormone membran
1-3 jam = Ekspresi gen
(ABA, CK, ETH,
IAA, dan GA)
> 12 jam=
Drought ABA+ETH+CK ; GA+IAA morfogenik
Flood GA+IAA
Morfogenik
ITMT/S1/SAN
Plant Response to Water Stress
C. Mechanisms of Plant Tolerance to Water Stress/Flood
Root
O2 Transfer Formatio
from Shoot to n on the
Roots stem Tolerance of Toxin
Accumulation
Inhibition of
Toxins- Glycolysis
producing General
Enzymes Decreased
Induction of Rate of
Toxins- Metabolism Maintenance
destroying of Aerobic
Enzymes Apparatus
ITMT/S1/SAN
Plant Response to Water Stress
E. Variabel of Plant Response to Water Stress
Morphology : Abscision index, root growth, and
component of yield.
ITMT/S1/SAN
Applied on Research Program of Water Stress
Efficiency :
a. Energy Convertion Efficiency of Some Forage Crop
= [input energy / output energy]x100%
b. Metabolisme (C, N, etc.) Efficiency of Some Forage
Crop [Photosynthesis/Transpiration Index], [Stomata]
[Proline, Serine, Glycine, Betaine], NR Activity Index.
Tolerance :
Selection of Some Forage Crop to Drought and
Waterlogging/Flooding Stress
ITMT/S1/SAN
Plant Response to Nutrient Stress
10% of the plant dry weight ( 1.5% of the fresh) is minerals
Deficiency
Deficiency Stress Toxicity
?
(1). The content of soil solution (Balancing); (2). The soil pH ;
(3). Availability to the plant ; (4). Translocation and
(5). Metabolic pathways
Tissue Concentration
Plant Response to Nutrient (Toxic)Stress
Acid (Al3+) and Salin (NaCl) Condition
1 1
2 2
Al(OH)+2
3 3
pAl 4 4
Al +3
5 5
6 6
7 Al(OH) 2+ 7
Al(OH) 4-
8 8
9 9
10 10
3 4 5 6 7 8
pH
I. Plant Response to Acid (Al3+) Stress
A. Sequence of Event in Injury During Stress
Absorp Exchange
Competition Enzyme
of Water
with Other Inactivation
Structure
Essensial
Element Exchange Position
with Other Essensial
Element
RootInhibition
Root Inhibition
I. Plant Response to Acid (Al3+) Stress
B. Mechanisms of Plant Tolerance to Acid (Al3+) Stress
External/Exclusion/Apoplasm
Absorption Excudate of
Anion > Cation Chelator
Preference of Electric Potential
NO3 and NH4
- +
Mucilage
Synthesis
Decarboxilation
(HCO3-) efflux NR Plasmamembrane
Selectivity
Induction of Organic
Compound
Al
pH-barier Immobilisation
Rhizosphere
Decrease of Al Toxicity
I. Plant Response to Acid (Al3+) Stress
B. Mechanisms of Plant Tolerance to Acid (Al3+) Stress
Internal/Inclusion/Symplasm
Al-Compartmention
in Vacuola
Absorption Preference of
Anion > Cation NO3- and NH4+
Decarboxilation
(HCO3-) efflux NR
Al-complex
- Organic Acids
- Protein/Enzyme
Decrease of Al Toxicity
I. Plant Response to Acid (Al3+) Stress
C. Mechanisms of Phytohormone to Acid (Al3+) Stress
0-15’ = mempengaruhi
permeabilitas
Acid (Al3+)
Phytohormone membran
Stress 1-3 jam = Ekspresi gen
(CK, IAA, & ETH)
If > 12 jam=
Morfogenik
NO3- CK dan IAA
NH4+ CK dan IAA
Tryptophane IAA
Cu, Fe ; Ca ETH
Morfogenik
ITMT/S1/SAN
II. Plant Response to Salt Stress
Salinitas = terdapatnya garam-garam dalam kosentrasi yang berlebihan
sehingga menekan pertumbuhan
Na+, Cl- dan SO4-2 Menginduksi defisiensi Ca+2, K+
Pendekatan
Floem and
Toxin
Growth Membrane
Inhibition Transport
Cell
Breakdown
DEATH
DEATH
ITMT/S1/SAN
II. Plant Response to Salt (Na+) Stress
B. Mechanisms of Plant Tolerance to Salt (Na+) Stress
Morphology Mechanisms by
(Water Content Balancing)
Leaf Size
Reduce Succulence
[Stomata] Increase
Earlier of Root
Reduce Lignification
Transpiration Cuticula Thickness
Reduce
Decrease of Na Toxicity
II. Plant Response to Salt (Na+) Stress
B. Mechanisms of Plant Tolerance to Salt (Na+) Stress
Physiology Mechanisms by
(Osmoregulation, Solutes & Membrane)
Osmoregulation
Secretion and
Membrane
Transport Compartmenta
Integrity
System tion of Na+
Ions and
Solutes
K+, Ca2+, Transport
Accumulation
Organic System
Compounds,
Osmotic Free Amino Lignification
Decrease Acids, and Leaf and
Sugars Vacuola
Decrease of Na Toxicity
II. Plant Response to Salt (Na+) Stress
C. Mechanisms of Phytohormone to Salt (Na+) Stress
0-15’
= Mempengaruhi
permeabilitas
Salt Stress Phytohormone membran
1-3 jam = Ekspresi gen
(ABA, CK, ETH,
IAA, dan GA)
> 12 jam=
Morfogenik
Osmotic ABA+ETH+CK ; GA+IAA
Morfogenik
ITMT/S1/SAN
II. Plant Response to Salt (Na+) Stress
C. Proposed Accumulation of Some Metabolic Under
Salt (Na+) Condition/Stress
Tyrosine +
Free Amino Acids Aspartic +
Phenylalanine Amide
Glutamic Acid
Tolerance :
Selection of Some Forage Crop to Salt (Na+) Stress
by Poliploidy and/or Protoplasma Fusion
ITMT/S1/SAN
Program Perbaikan THMT
Efisiensi
Sifat Daya & Kualitas
Toleransi
- Seleksi
- Fusi Protoplas
- Poliploidi
- Transfer Gen
- Komponen Produksi
- Produksi dan Produktifitas
- Nilai Nutrisi
ITMT/S1/SAN
ZAT PENGATUR TUMBUH = ZPT
(Hormon Tanaman)
1. Pembelahan sel
TUMBUH
2. Pembesaran sel
Tanaman
(Multiselluler) 3. Differensiasi BERKEMBANG
PENGATURAN OLEH
SISTEM-SISTEM PESAN KIMIAWI
“Systems of Chemical Messenger”
HORMON TANAMAN
ITMT/S1/SAN
ZAT PENGATUR TUMBUH = ZPT
(Hormon Tanaman)
Hormon Tanaman =
Senyawa organik yang disintesa oleh organ tanaman ttt, lalu
ditranslokasikan ke bagian tanaman lainnya, yang dalam dosis/
konsentrasi sangat rendah mempengaruhi respon fisiologi
tanaman
MEKANISME KERJA =
Sebagai second messenger untuk memperbesar/menstimulir
rantai metabolisme berikutnya/lainnya
ITMT/S1/SAN
ZAT PENGATUR TUMBUH = ZPT
(Macam, Biosintesis, Peran)
1. AUKSIN
Sebagai hormon pertumbuhan universal, sehingga tanpa auksin
TIDAK AKAN TERJADI PERTUMBUHAN.
Misalnya : CH2COOH
IAA = Indole Acetic Acid
NAA = Naphtalene Acetic Acid NH
Peranan :
a. Perangsangan sintesis mRNA (sintesis asam amino/enzim)
b. Pemanjangan batang
c. Dominansi apikal
d. Fototropisme
e. Merangsang pertumbuhan akar, bunga dan buah
f. Menunda absisi (penguguran daun)
ITMT/S1/SAN
ZAT PENGATUR TUMBUH = ZPT
(Macam, Biosintesis, Peran)
2. Gibberelin (GA)
Ditemukan oleh Gibberella Fujikuroi, sebagai hormon tumbuh
untuk pemanjangan batang dan daun padi
O
Misalnya :
GA3 = Gibberelic Acid turunan-3 OH
OH
GA12 = Gibberelic Acid turunan-12 = CH
COOH 2
Peranan :
a. Pembelahan dan pembesaran sel
b. Pemanjangan batang dan daun
c. Mematahkan dormansi
d. Perkecambahan (merangsang enzim-enzim hidrolisis)
e. Mencegah degradasi klorofil
f. Rasio jenis kelamin tanaman
ITMT/S1/SAN
ZAT PENGATUR TUMBUH = ZPT
(Macam, Biosintesis, Peran)
3. Sitokinin NH-CH2-CH=C=C2H5OH
Peranan :
a. Pembelahan sel (khususnya proses sitokinesis/sitoplasma)
b. Perluasan/Lebar daun
c. Bersama auksin untuk differensiasi mata tunas
d. Menunda penuaan (senescen)
e. Komponen struktural RNA, sehingga berperanan penting
dalam proses sintesis asam amino/enzim/protein.
ITMT/S1/SAN
ZAT PENGATUR TUMBUH = ZPT
(Macam, Biosintesis, Peran)
4. Etilen (CH2=CH2)
Berupa gas hasil alami selama proses pemasakan buah/biji
Peranan :
a. Merangsang pemasakan buah
b. Pembesaran batang secara radial/ke samping
c. Merangsang absisi / gugur
d. Merangsang pembungaan
e. Pematahan dormansi
ITMT/S1/SAN
ZAT PENGATUR TUMBUH = ZPT
(Macam, Biosintesis, Peran)
5. Inhibitor
Inhibitor mempunyai fungsi utama sebagai penghambat
pertumbuhan
Misalnya :
Asam Absisik (ABA), Retardant, dll.
Peranan :
a. Penuaan sel
b. Menghambat sintesis RNA, sehingga sintesis asam-asam
amino/enzim/protein terhambat
c. Mengatur dormansi (bersama dengan GA)
Dormansi terjadi bila ABA dan GA ; juga sebaliknya.
d. Menahan aktivitas GA
e. Pematahan dormansi
ITMT/S1/SAN
ZAT PENGATUR TUMBUH = ZPT
(Macam, Biosintesis, Peran)
1 Dormansi
2 Perakaran
3 Laju Pertumbuhan
4 Pembungaan
5 Penentuan sex
6 Pembentukan buah
7 Penuaan sel/Gugur
8 Sintesis mRNA
ITMT/S1/SAN