ABSTRAK

Pengaruh Suplementasi Vitamin E

Terhadap Fungsi Oxidative Burst Sel Netrofil Pada Usia Lanjut

Pertiwi Rosanti

Latar Belakang : Immunosenescence berdampak pada sistem imun alamiah yaitu

penurunan fungsi oxidative burst netrofil. Studi terdahulu menyatakan peran vitamin E
terhadap oxidative burst netrofil melalui jalur asam arakidonat. Belum ada penelitian
mengenai pengaruh pemberian vitamin E terhadap fungsi oxidative burst sel netrofil pada
usia lanjut.

Tujuan : Mengetahui pengaruh vitamin E pada oxidative burst sel netrofil pada usia
lanjut

Metode : Desain studi adalah randomized controlled trial, single blind, pre and post test
control group. Diikuti 28 individu usia lanjut yang memenuhi kriteria inklusi dan
eksklusi. Fungsi oxidative burst diukur sebelum dan setelah pemberian vitamin E atau
plasebo selama 7 hari. Stimulan oxidative burst adalah PMA dan interpretasi fungsi
menggunakan flowsitometri.

Hasil : Didapatkan sebagian besar subyek adalah wanita, rerata usia 73 tahun, dengan
jumlah lekosit dan netrofil dalam batas normal. Rerata fungsi oxidative burst netrofil
sebelum perlakuan pada kelompok vitamin E 76,6413,98%, sedangkan kelompok
plasebo 80,3212,08% (N 98-100%). Hasil sesudah perlakuan pada kelompok vitamin E
87,7911,25%, sedangkan kelompok plasebo 81,9218,21%. Hasil uji pra dan pasca
perlakuan pada kelompok vitamin E menunjukkan perbedaan yang bermakna (p = 0,011,
Wilcoxon Signed Rank Test). Sedangkan perubahan fungsi oxidative burst antar kedua
kelompok tidak bermakna secara statistik (11,1514,9% vs 1,6019,46).

Kesimpulan : Terdapat kenaikan fungsi oxidative burst pada usia lanjut setelah
diberikan asupan vitamin E selama tujuh hari.

Kata Kunci : Immunosenescence, Usia Lanjut, Netrofil, Oxidative Burst, Vitamin E

7
 ABSTRACT

Effect of Vitamin E Supplementation

Against Neutrophils Oxidative Burst In Elderly

Pertiwi Rosanti

Background : Immunosenescence has an impact on innate immune system as a

decreasing of the neutrophils oxidative burst . Past studies have claimed the role of
vitamin E on the neutrophils oxidative burst through the arachidonic acid pathway. But,
there are still no studies about the effect of vitamin E on oxidative burst of neutrophils in
the elderly .

Objective: To determine the effect of vitamin E on neutrophils oxidative burst in elderly

Methods : The study design is a randomized controlled trial, single-blind, pre and post
test control group. Followed by 28 elderly who met the inclusion and exclusion criteria.
Oxidative burst function was measured before and after treatment (vitamin E or a
placebo) for 7 day. The oxidative bursts stimulant is PMA and interpretation functions
using flowsitometri .

Results: Most of the subjects were female, mean age 73 years, with the number of
leukocytes and neutrophils within normal limits. The mean oxidative burst before
treatment in the vitamin E group 76.64 13.98 %, while the placebo 80.32 12.08 % ( N
98-100 % ). Results after treatment in the vitamin E group 87.79 11.25 %, while the
placebo 81.92 18.21 % (pre-post test, p = 0.011, wilcoxon sign rank test). While the
increase in oxidative burst in the vitamin E group is greater than the placebo ( 14.9 %
11.15 vs 19.46 1.60 ) but not significantly different between those group.

Conclusion : There was an increase in oxidative burst in the elderly after vitamin E
intake for seven days.

Keywords : Immunosenescence, Elderly, Neutrophils, Oxidative Burst, Vitamin E