SCREENING

5/8/2017

Pengenceran Simplo Duplo Rerata Konversi Koloni Teoritis

10 0 TBUD TBUD 645000000 6450000
10 -1 TBUD TBUD 64500000 645000
10 -2 TBUD TBUD 6450000 64500
10 -3 TBUD TBUD 645000 6450
10 -4 TBUD TBUD 64500 645
10 -5 1496 1536 1516 6450 64.5
10 -6 331 354 342.5 645 6.45
10 -7 69 60 64.5 64.5 0.645
10 -8 8 11 9.5
10 -9 3 2 2.5
10 -10 0 0 0
PRA-VERIFIKASI
5/16/2017

Perlakuan Simplo Duplo

Ulangan
Only Sampel 0 0
Only Spike 10 -4 1 mL 738 728
1 Sampel+spike 10 -4 1 mL 656 760
2 Sampel+spike 10 -4 1 mL 641 641
3 Sampel+spike 10 -4 1 mL 699 667
Rerata 677
Only Spike 10 -4 0,5 mL 482 488
1 Sampel+spike 10 -4 0,5 m 517 458
2 Sampel+spike 10 -4 0,5 m 443 415
3 Sampel+spike 10 -4 0,5 m 442 443
Rerata 453
Only Spike 10 -5 1 mL 67 66
1 Sampel+spike 10 -5 1 mL 75 87
2 Sampel+spike 10 -5 1 mL 71 87
3 Sampel+spike 10 -5 1 mL 119 96
Rerata 89
Kontrol Awal 0
Kontrol Akhir 0
VERIFIKASI
5/18/2017

Perlakuan Simplo Duplo

Ulangan
Only Sampel 0 0
Only Spike 10 -5 2mL 131 135
1 Sampel+spike 10 -5 2mL 188 165
2 Sampel+spike 10 -5 2mL 129 126
3 Sampel+spike 10 -5 2mL 204 171
4 Sampel+spike 10 -5 2mL 140 138
5 Sampel+spike 10 -5 2mL 139 129
6 Sampel+spike 10 -5 2mL 119 123
7 Sampel+spike 10 -5 2mL 183 168
8 Sampel+spike 10 -5 2mL 194 209
9 Sampel+spike 10 -5 2mL 109 87
10 Sampel+spike 10 -5 2mL 125 120
11 Sampel+spike 10 -5 2mL 120 125
12 Sampel+spike 10 -5 2mL 156 140
13 Sampel+spike 10 -5 2mL 134 129
14 Sampel+spike 10 -5 2mL 141 120
15 Sampel+spike 10 -5 2mL 135 140
Rerata 148 139
Only Spike 10 -4 0,25mL 247 278
1 Sampel+spike 10 -4 0,25mL 276 218
2 Sampel+spike 10 -4 0,25mL 267 193
3 Sampel+spike 10 -4 0,25mL 269 285
4 Sampel+spike 10 -4 0,25mL 252 284
5 Sampel+spike 10 -4 0,25mL 242 275
6 Sampel+spike 10 -4 0,25mL 265 238
7 Sampel+spike 10 -4 0,25mL 212 217
8 Sampel+spike 10 -4 0,25mL 204 220
9 Sampel+spike 10 -4 0,25mL 290 253
10 Sampel+spike 10 -4 0,25mL 227 277
11 Sampel+spike 10 -4 0,25mL 246 262
12 Sampel+spike 10 -4 0,25mL 222 258
13 Sampel+spike 10 -4 0,25mL 258 218
14 Sampel+spike 10 -4 0,25mL 272 290
15 Sampel+spike 10 -4 0,25mL 264 264
Rerata 251.0667 250
Kontrol Awal 0
Kontrol Akhir 0
 PERHITUNGAN AKURASI
Verifikasi TPC pada produk RTD HB (spike pengenceran 10 -5 volume 2 mL)

Ulangan Simplo (Sa+Sp)-Sa Duplo (Sa+Sp)-Sa Log A Log B Rata-rata

1 188 188 165 165 2.2742 2.2175 2.2458
2 129 129 126 126 2.1106 2.1004 2.1055
3 204 204 171 171 2.3096 2.2330 2.2713
4 140 140 138 138 2.1461 2.1399 2.1430
5 139 139 129 129 2.1430 2.1106 2.1268
6 119 119 123 123 2.0755 2.0899 2.0827
7 183 183 168 168 2.2625 2.2253 2.2439
8 194 194 209 209 2.2878 2.3201 2.3040
9 109 109 87 87 2.0374 1.9395 1.9885
10 125 125 120 120 2.0969 2.0792 2.0880
11 120 120 125 125 2.0792 2.0969 2.0880
12 156 156 140 140 2.1931 2.1461 2.1696
13 134 134 129 129 2.1271 2.1106 2.1188
14 141 141 120 120 2.1492 2.0792 2.1142
15 135 135 140 140 2.1303 2.1461 2.1382
Only Sampel 0 0
Only Spike 131 135 135 2.1173 2.1303 2.1238
Min
Max
Rata-rata

PERHITUNGAN AKURASI
Verifikasi TPC pada produk RTD HB (spike pengenceran 10 -4 volume 0,25 mL)

Ulangan Simplo (Sa+Sp)-Sa Duplo (Sa+Sp)-Sa Log A Log B Rata-rata

1 276 276 218 218 2.4409 2.3385 2.3897
2 267 267 193 193 2.4265 2.2856 2.3560
3 269 269 285 285 2.4298 2.4548 2.4423
4 252 252 284 284 2.4014 2.4533 2.4274
5 242 242 275 275 2.3838 2.4393 2.4116
6 265 265 238 238 2.4232 2.3766 2.3999
7 212 212 217 217 2.3263 2.3365 2.3314
8 204 204 220 220 2.3096 2.3424 2.3260
9 290 290 253 253 2.4624 2.4031 2.4328
 10 227 227 277 277 2.3560 2.4425 2.3993
11 246 246 262 262 2.3909 2.4183 2.4046
12 222 222 258 258 2.3464 2.4116 2.3790
13 258 258 218 218 2.4116 2.3385 2.3750
14 272 272 290 290 2.4346 2.4624 2.4485
15 264 264 264 264 2.4216 2.4216 2.4216
Only Sampel 0 0
Only Spike 247 278 2.3927 2.4440 2.4184
Min
Max
Rata-rata

Keterangan
Standar keberterimaan % Recovery yaitu:
AOAC 70%
SIC LAS 80%
Nutrifood 100 ± 20 %
ume 2 mL)

% Recovery
105.75
99.14
106.95
100.90
100.14
98.07
105.65
108.48
93.63
98.32
98.32
102.16
99.77
99.55
100.68

93.63
108.48
101.17

me 0,25 mL)

% Recovery
98.81
97.42
100.99
100.37
99.72
99.24
96.40
96.18
100.59
 99.21
99.43
98.37
98.21
101.25
100.13

96.18
101.25
99.09
 PERHITUNGAN PRESISI
Verifikasi TPC pada produk RTD HB (spike pengenceran 10 -5 volume 2 mL)

Ulangan Simplo-sampel log S Duplo-sampel log D x log Difference (log S-log D)

1 188 2.27416 165 2.21748 2.24582 0.05667
2 129 2.11059 126 2.10037 2.10548 0.01022
3 204 2.30963 171 2.23300 2.27131 0.07663
4 140 2.14613 138 2.13988 2.14300 0.00625
5 139 2.14301 129 2.11059 2.12680 0.03243
6 119 2.07555 123 2.08991 2.08273 -0.01436
7 183 2.26245 168 2.22531 2.24388 0.03714
8 194 2.28780 209 2.32015 2.30397 -0.03234
9 109 2.03743 87 1.93952 1.98847 0.09791
10 125 2.09691 120 2.07918 2.08805 0.01773
11 120 2.07918 125 2.09691 2.08805 -0.01773
12 156 2.19312 140 2.14613 2.16963 0.04700
13 134 2.12710 129 2.11059 2.11885 0.01652
14 141 2.14922 120 2.07918 2.11420 0.07004
15 135 2.13033 140 2.14613 2.13823 -0.01579

PERHITUNGAN PRESISI
Verifikasi TPC pada produk RTD HB (spike pengenceran 10 -4 volume 0,25 mL)

Ulangan Simplo-sampel log S Duplo-sampel log D x log Difference (log S-log D)

1 276 2.44091 218 2.33846 2.33846 0.10245
2 267 2.42651 193 2.28556 2.28556 0.14095
3 269 2.42975 285 2.45484 2.45484 -0.02509
4 252 2.40140 284 2.45332 2.45332 -0.05192
5 242 2.38382 275 2.43933 2.43933 -0.05552
6 265 2.42325 238 2.37658 2.37658 0.04667
7 212 2.32634 217 2.33646 2.33646 -0.01012
8 204 2.30963 220 2.34242 2.34242 -0.03279
9 290 2.46240 253 2.40312 2.40312 0.05928
10 227 2.35603 277 2.44248 2.44248 -0.08645
11 246 2.39094 262 2.41830 2.41830 -0.02737
12 222 2.34635 258 2.41162 2.41162 -0.06527
13 258 2.41162 218 2.33846 2.33846 0.07316
14 272 2.43457 290 2.46240 2.46240 -0.02783
15 264 2.42160 264 2.42160 2.42160 0.00000
Keterangan
Standar keberterimaan % RSD <0,1% dan % CV <10%
-5 volume 2 mL)

Diff/mean Sqr (diff/mean)

0.02524 0.00064
0.00485 0.00002
0.03374 0.00114
0.00292 0.00001
0.01525 0.00023
-0.00689 0.00005
0.01655 0.00027
-0.01404 0.00020
0.04924 0.00242
0.00849 0.00007
-0.00849 0.00007
0.02166 0.00047
0.00779 0.00006
0.03313 0.00110
-0.00739 0.00005
SUM 0.00681
RSD 0.01507
%CV 1.51

volume 0,25 mL)

Diff/mean Sqr (diff/mean)

0.04381 0.00192
0.06167 0.00380
-0.01022 0.00010
-0.02116 0.00045
-0.02276 0.00052
0.01964 0.00039
-0.00433 0.00002
-0.01400 0.00020
0.02467 0.00061
-0.03540 0.00125
-0.01132 0.00013
-0.02706 0.00073
0.03129 0.00098
-0.01130 0.00013
 0.00000 0.00000
SUM 0.01122
RSD 0.01934
%CV 1.93
 Perhitungan Ketidakpastian Pengukuran
Verifikasi TPC pada produk RTD HB (spike pengenceran 10 -5 volume 2 mL)
Jumlah Bakteri Jumlah Bakteri dalam Log
No Simplo Duplo Log Simplo Log Duplo Rata-rata
1 188 165 2.27416 2.21748 2.24582
2 129 126 2.11059 2.10037 2.10548
3 204 171 2.30963 2.23300 2.27131
4 140 138 2.14613 2.13988 2.14300
5 139 129 2.14301 2.11059 2.12680
6 119 123 2.07555 2.08991 2.08273
7 183 168 2.26245 2.22531 2.24388
8 194 209 2.28780 2.32015 2.30397
9 109 87 2.03743 1.93952 1.98847
10 125 120 2.09691 2.07918 2.08805
11 120 125 2.07918 2.09691 2.08805
12 156 140 2.19312 2.14613 2.16963
13 134 129 2.12710 2.11059 2.11885
14 141 120 2.14922 2.07918 2.11420
15 135 140 2.13033 2.14613 2.13823
a. Range data 0.31550
b. Median data 2.12680
c. Mean data 2.14856
d. Standar deviasi 0.084527
e. Variansi sampel 0.007145
f. RSD 0.015
g. Ketidakpastian (U) 0.16905 2
h. Jumlah Bakteri 2,14856 ± 0.16905

Perhitungan Ketidakpastian Pengukuran

Verifikasi TPC pada produk RTD HB (spike pengenceran 10 -4 volume 0,25 mL)
Jumlah Bakteri Jumlah Bakteri dalam Log
No Simplo Duplo Log Simplo Log Duplo Rata-rata
1 276 218 2.44091 2.33846 2.38968
2 267 193 2.42651 2.28556 2.35603
3 269 285 2.42975 2.45484 2.44230
4 252 284 2.40140 2.45332 2.42736
5 242 275 2.38382 2.43933 2.41157
6 265 238 2.42325 2.37658 2.39991
7 212 217 2.32634 2.33646 2.33140
8 204 220 2.30963 2.34242 2.32603
9 290 253 2.46240 2.40312 2.43276
10 227 277 2.35603 2.44248 2.39925
11 246 262 2.39094 2.41830 2.40462
 12 222 258 2.34635 2.41162 2.37899
13 258 218 2.41162 2.33846 2.37504
14 272 290 2.43457 2.46240 2.44848
15 264 264 2.42160 2.42160 2.42160
a. Range data 0.12246
b. Median data 2.39991
c. Mean data 2.39634
d. Standar deviasi 0.037479
e. Variansi sampel 0.001405
f. RSD 0.019
g. Ketidakpastian (U) 0.07496
h. Jumlah Bakteri 2,39634 ± 0.07496