I.

Tujuan Percobaan

II. Prinsip Percobaan

III. Teori Dasar

IV. Prosedur Percobaan

V. Data Pengamatan dan Perhitungan

5.1 Obat Monokompartemen

Tabel 5.1.1. Kadar Plasma Obat Monokompartemen

Waktu (jam) Ct (μg/mL) LnCp

a = -0,0514
0.5 0.699 -0.358104537
b = -0,459
1 0.622 -0.474815186 r = 0,9984
2 0.413 -0.884307686 Persamaan Regresi :
2.5 0.292 -1.231001477 y = -0459x-0,0514
4 0.152 -1.883874758
6 0.06 -2.813410717
8 0.024 -3.729701449

Persamaan Farmakokinetik :

Ln Cp = Ln Cp⁰ - k.t
Ln Cp = 0,0514 – 0,459.t

Tabel 5.1.2. Parameter Farmakokinetik

Parameter Farmakokinetik Hasil

Ke (/jam) 0.459
t½(jam) 1.509803922
Ln C₀ 0.0514
C₀ 1.052743907
Dosis I.V (mg) 160
Vd (mL) 151.9837816
Cl (mL/jam) 69.76055576
AUC₀~ (μg/mL.jam) 2.293559709
Penguraian perhitungan parameter farmakokinetik

Ke = nilai b = nilai r dari persamaan regresi = 0,459/jam

0,693
t½ = = 1,5098 jam
0,459/ jam

Ln Co = nilai a = 0,0514

Co = antiLn nilai a = 1,0527 μg/mL

D 0b 160
Vd = 0 = = 151,9837 mL
Cp 1,5027

Cl = Vd. Ke = 151,9837 mL x 0,459/jam = 69,7605 mL/jam

Co 160
AUC = = = 2,2935 μg/mL.jam
0 Ke. Vd 0,459 x 151,9837

V.2 Obat Multikompartemen

Tabel 5.2.1 Kadar Plasma Obat MultiKompartemen

waktu Kadar Obat dalam

Ln Cp Ln C' C' Cr Ln Cr
(menit) darah (µg/mL)

0.25 53.8 3.9853 2.7966 16.7966 37.4099 3.6219

0.5 43.3 3.7682 2.7652 15.883 27.4169 3.3112
0.75 35 3.5553 2.7338 15.3916 19.6083 2.9759
1 29.1 3.3707
1.5 21.2 3.054
2 17 2.8332
3 12.6 2.5337
4 10.5 2.3514
5 9 2.1972
6 8 2.0794
7 7 1.9459
 Tabel 5.2.2 Persamaan regresi dan parameter farmakokinetik

Persamaan Parameter Hasil

y = bx + a α 1.292
y = -1.292x + 3.99 Ln A 3.949
A 51.8834
y = bx + a β 0.1257
y = -0.1257x + 2.8281 Ln B 2.8281
B 16.9133

Persamaan Farmakokinetik Multikompartemen:

Ln Cp = Ln A-a.t + Ln B-b.t  
Ln Cp = 3.949-1.292.t + 2.8281-0.1257.t

Tabel 5.2.3 Parameter Farmakokinetik

Parameter
Hasil
Farmakokinetik
₁₂
K₂₁(/jam) 0.3937
K (/jam) 0.6114
K (/jam) 0.4124
t 1/2 5.5131
₀
Vp ( mL) 0.7267
AUC ~ (µg/mL.jam) 174.71
Cl (mL/jam) 0.2861
Penguraian perhitungan :

a. Perhitungan nilai Ln C’ dan C’ (C ekstrapolasi)

 t 0,25 menit
Ln C’ = Ln B – k.t
Ln C’ = 2,8281 – 0,1257 . 0,25
Ln C’ = 2,8281 – 0,0314
Ln C’ = 2,7967
C’ = antiLn 2,7967
 C’ = 16,7966
 t 0,5 menit
Ln C’ = Ln B – k.t
Ln C’ = 2,8281 – 0,1257 . 0,5
Ln C’ = 2,8281 – 0,0628
Ln C’ = 2,7653
C’ = AntiLn 2,7653
C’ = 27,4169
 t 0,75 menit
Ln C’ = Ln B – k.t
Ln C’ = 2,8281 – 0,1257 . 0,75
Ln C’ = 2,8281 – 0,0943
Ln C’ = 2,7338
C’ = AntiLn 2,7338
C’ = 19,6083

b. Perhitungan Cr (C residual)
 t 0,25 menit
Cr = Cp – C’
Cr = 53,8 – 16,7966
Cr = 37,4099
 t 0,5 menit
Cr = Cp – C’
Cr = 43,3 –15,8830
Cr = 27,4169
 t 0,75 menit
Cr = Cp – C’
Cr = 35 – 15,396
Cr = 19,6083
c. Perhitungan K
αβ ( A+ B)
K=
( Aβ )+(Bα )
1,292.0,1257(51,8834+16,9133)
K=
( 51,8834.0,1257 )+(16,9133.1,292)
0,1624(68,7967)
K=
6,5217+21,8519
11,1725
K= = 0,3937/jam
28,3736
d. Perhitungan K12
AB(β +α )2
K12 =
( A +B )+( Aβ+ Bα )
51,8834.16,9133(0,1257−1,292)2
K12 =
( 51,8834+16,9133 ) (51,8834.0,1257+16,9133.1,292)
877,5195(1,3602)
K12 =
68,7967 . 28,3736
1193,6020
K12 = = 0,6114/jam
1952,0100
e. Perhitungan K21
Aβ +B α
K21 =
A+ B
51,8834.0,1257+ 16,9133.1,292
K21 =
51,8834+ 16,9133
6,5217+21,8519
K21 =
68,7967
28,3736
K21 = = 0,4124/jam
68,7967

f. Perhitungan t ½

0,693
t½=
k
 0,693
t½= = 5,5131 jam
0,1257

g. Perhitungan Vp

D°
Vp =
A +B

50
Vp = = 0,7267 mL
51, 8834+ 16,9133

h. Perhitungan Cl

Cl = Vp.K

Cl = 0,7267.0,3937 = 0,2861 mL/jam

i. Perhitungan AUC₀~
D°
AUC₀~ =
K . Vp
50
AUC₀~ =
0,3937.0,7267
50
AUC₀~ = = 174,7640 mg/mL.jam
0,2861