BIOKIMIA DASAR (3sks)

Minda Azhar

Pendahuluan
Struktur sel dan Virus
Air pada sistem biologi
Karbohidrat
Protein
Vitamin dan mineral
Enzim
Lipd dan Membran
Asam Nukleat
Hormon
Presentasi tugas terbuka

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Protein

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 Apakah sutera, protein?
Bagaimana sifat sutera?

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 Protein
• “Proteus” utama
• Polimer asam amino
• Residu asam amino berikatan dengan residu
asam amino lainnya melalui ikatan peptida
• Dapat dihidrolisis

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 Bentuk-bentuk protein

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 Protein terbaru di PDB
(September 2020)

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 • Struktur protein, sifat protein, fungsi protein,
kaitannya ?

• Dipeptida, Oligopeptida, Polipeptida,

bedanya?

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 Beberapa fungsi protein
(Mengapa fungsinya berbeda?)

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 Pengelompokan protein

1.Berdasarkan fungsi yaitu

enzyme ,contoh inulinase, amylase
p.transport, contoh hemoglobin
p.nutrient dan penyimpan, contoh kasein, feritin
p.kontraktil atau motil, contoh miosin, aktin
p.struktural, contoh keratin
p.pertahanan, contoh antibodi, bisa ular
p.pengatur, contoh insulin, hormon tumbuh
2.Berdasarkan bentuk (p.globular dan p.serabut)
3.Berdasarkan ada tidaknya gugus lain (p.sederhana dan
p.konyugasi)
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Asam amino bukan pembentuk protein

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 Sifat asam amino

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 Pembentukan peptida

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 Peptida
dan penamaannya
The pentapeptide
serylglycyltyrosylalanylleucine, or
Ser–Gly–Tyr–Ala–Leu

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 Practice problem

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 Sudut ikatan

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 Levels of protein structure

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 Levels of protein structure

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 Levels of protein structure

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 Struktur primer

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 Struktur sekunder protein

The most common secondary structures are the

α helix, the β conformation, and β turns

Pauling and Corey predicted the existence of these secondary

structures in 1951, several years before the first complete protein
structure was elucidated.

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 Struktur sekunder
α-helix

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 Struktur sekunder
α-helix

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 Ikatan hidrogen pada struktur sekunder protein

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 β Turns Are Common in Proteins
In globular proteins, which have a compact folded
structure, nearly one-third of the amino acid
residues are in turns or loops where the
polypeptide chain reverses direction

These are the connecting elements that link

successive runs of α-helix or β-conformation

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 Struktur sekunder
β-sheet

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 Struktur sekunder
β-sheet

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 The β-Conformation Organizes Polypeptide Chains
into β Sheets

Pauling and Corey predicted

a second type of repetitive structure, the beta conformation

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 Struktur tersier β hemoglobin

• Tunjukkanlah
α-helix ?
β-sheet ?
β-turn ?

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 Struktur tersier
myoglobin
(protein penyimpan oksigen diotot)

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Dari data apa Struktur tersier protein

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 Struktur tersier
α-lactalbumin

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 Struktur tersier
myoglobin

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 Struktur tersier
myoglobin dan β-subunit dari hemoglobin

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 Interaksi pada struktur tersier
(interaksi antara rantai samping)

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 Struktur quartener
gabungan beberapa polipeptida untuk menghasilkan suatu protein fungsional

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 Struktur quartener

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 Protein : Native dan denaturasi

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 Pengaruh suhu pada struktur protein
(Denaturasi protein, dan koagulasi protein)

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 Pengaruh pH pada struktur protein
(Denaturasi protein, dan koagulasi protein)

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Terdenaturasikah? Terkoagulasikah ?

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 Sel darah merah normal dan tidak normal

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 Struktur sekunder dan sifat protein serat

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 Struktur serat sutera
β-sheets

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Struktur collagen

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 Data Base Protein

• Protein Data Bank (PDB)

Informasi struktur protein apakah yang terbaru pada
tahun ini?

• GenBank
Berapakah jumlah informasi insulin dan dimanakah
sumbernya?

• CAZY
Berapa family enzim ini sekarang

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 Pemisahan protein berdasarkan berat molekul

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 Memperkirakan berat molekul protein

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