Journal of Pharmacy Technology preparations Sterile Sriwijaya University 2017

CALIBRATION OF LABORATORY GLASSWARE AND PUMPKIN

MEASURE IN MAKING STOCKS STERILE

Cahyani Putri Ngulwiyah, Ina Suci Pratiwi, Iwan Santoso

of Pharmacy Faculty of Mathematics and Natural Sciences,

University of Sriwijaya
Email: farmasiunsri2015@gmail.com

ABSTRACT

Kalibrasi mempunyai pengertian sebagai rangkaian kegiatan membandingkan

hasil pengukuran suatu alat dengan alat standard yang sesuai untuk menentukan besarnya
koreksi pengukuran alat serta ketidak pastian nya. Alat ukur yang telah dikalibrasi tidak
akan secara terus- menerus berlaku masa kalibrasi nya, karena peralatan tersebut selama
masa penggunaan nya pasti mengalami perubahan spesifikasi akibat pengaruh frekuensi
pemakaian, lingkungan penyimpanan, cara pemakaian, dan sebagainya. Hasil pengukuran
yang diberikan oleh beberapa alat sejenis tidak selalu menunjukan hasil yang sama,
meskipun alat tersebut mempunyai tipe yang sama. Perbedaan ini diperbesar lagi dengan
adanya pengaruh lingkungan, operator, serta metode pengukuran. Agar setiap alat dapat
mmemberikan hasil ukur dengan keabsahan yang sama alat ukur tersebut perlu
mempunyai ketelusuran kepada standard nasional atau standard internasional. Cara untuk
memberikan jaminan bahwa alat yang digunakan mempunyai ketelusuran kepada
standard nasional adalah dengan melakukan kalibrasi terhadap alat tersebut.

Keywords: Calibration , Method, Autokaf, Oven, Bacteria

I. INTRODUCTION Secara umum kalibrasi

mempunyai pengertian sebagai

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rangkaian kegiatan membandingkan adanya pengaruh lingkungan, operator,

hasil pengukuran suatu alat dengan alat serta metode pengukuran. Padahal dalam
standard yang sesuai untuk menentukan menghasilkan hasil pengukuran tersebut
besarnya koreksi pengukuran alat serta sangat diharapkan bahwa setiap alat ukur
ketidak pastian nya. Dalam pengertian yang digunakan dimanapun memberikan
ini alat standard yang digunakan juga hasil ukur yang samadalam kaitan nya
harus terkalibrasi dibuktikna dengan dengan keperluan keamanan, kesehatan
sertifikat kalibrasi. dengan demikian transaksi, dan keselamatan (Aulton,
maka besarnya koreksi pengukuran alat 1994)
dapat ditelusurkan ke standard nasional
Agar setiap alat dapat
atau standard internasional dengan suatu
mmemberikan hasil ukur dengan
mata rantai kegiatan kalibrasi yang
keabsahan yang sama alat ukur tersebut
tidak terputus (Akers, 2010)
perlu mempunyai ketelusuran kepada
Alat ukur yang telah dikalibrasi
standard nasional atau standard
tidak akan secara terus- menerus
internasional. Cara untuk memberikan
berlaku masa kalibrasi nya, karena
jaminan bahwa alat yang digunakan
peralatan tersebut selama masa
mempunyai ketelusuran kepada standard
penggunaan nya pasti mengalami
nasional adalah dengan melakukan
perubahan spesifikasi akibat pengaruh
kalibrasi terhadap alat tersebut. Lebih
frekuensi pemakaian, lingkungan
dari itu untuk memelihara ketelusuran
penyimpanan, cara pemakaian, dan
tersebut perlu dilakukan perawatan alat
sebagainya. Untuk itulah selama
dalam selang waktu kalibrasi tertentu.
berlaku nya masa kalibrasi alat
Ketelusuran pengukuran tidak hanya
bersangkutan perlu dipelihara
sekedar menjadi penyaratan
ketelusuran nya dengan cara perawatan
administrative, melainkan telah menjadi
dan cek aatara secara periodic (Felton,
kebutuhan teknis yang mendasar
2012)
terutama dengan diwajibkannya
Hasil pengukuran yang diberikan mencantumkan utimasi ketidakpastian
oleh beberapa alat sejenis tidak selalu dalam hasil uji (Martin, 1993)
menunjukan hasil yang sama, meskipun
alat tersebut mempunyai tipe yang sama. Tujuan dilakukannya kalibrasi
Perbedaan ini diperbesar lagi dengan untuk mencapai ketelusuran

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 Journal of Pharmacy Technology preparations Sterile Sriwijaya University
pengukuran. Hasil pengukuran dapat di
kaitkan atau di telusur sampai kestandar
yang lebih tinggi atau teliti (standard
primer nasional dan atau internasional).
Melalui rangkaian perban dengan yang
tak terputus. Menentukan
(penyimpangan) kebenaran
konfesional penunjukkan
instrument ukur menjamin hasil-hasil
pengukuran sesuai dengan standard
nasional maupun sesuai
standard internasional (Martin, 1993)
Manfaat kalibrasi yakni menjaga
kondisi imstrumen ukur dan bahan ukur
agar tetap sesuai dengan spesifikasinya.
Untuk mendukung system mutu yang
diterapkan di berbagai industri pada
perawatan laboratorium dan produksi
yang dimiliki. Bisa mengetahui
perbedaan (penyimpangan) antara harga
benar dengan harga yang di tunjukkan
oleh alat ukur. Persyaratan kalibrasi
yakni standard acuan yang mampu
telusur ke standard nasional
standard internasional. Metode kalibrasi
yang di akui secara nasional atau
internasional, personil kalibrasi yang
terlatih, yang dibuktikan
sertifikasi laboratorium
terakredetasi. Ruangan atau tempat
kalibrasi yang terkondisi, seperti suhu,
kelembapan udara, aliran udara dan juga
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kedap getaran. Alat yang di kalibrasi
dalam keadaan berfungsi baik atau tidak
rusak (Akers, 2010)
II. RESEARCH METHODOLOGY
deriasi a. Place and Time
nilai
suatu Activities carried out in the
Laboratory of Pharmaceutical
Technology Sriwijaya University, on
dengan Tuesday, October 24, 2017 from 10:00
am until 18:00 pm
b. Equipment and Materials
The tools used in this experiment
include oven (1 piece), flask (1 piece),
measuring cups (1 piece), thermometers
(1 piece), analytical balance (1 piece),
calculator (1 piece), and weights (1 set).
Meanwhile 100ml distilled water
necessary materials
c. Procedure
atau
1) Calibration of the measuring cup
measuring cup washed and dried,
and then weighed measuring cup blank
is filled with distilled water to mark
dengan
boundaries, kept cool to 20 degrees
yang
Celsius, then weigh the measuring cup
berisi distilled water, measure and
record the weighing results, calculate
the volume of the average and standard
 Journal of Pharmacy Technology preparations Sterile Sriwijaya University 2017

deviation and tolerance. Repeat these 20 degrees Celsius, then weighed flask
steps 3x which berisi distilled water, measure
and record the results of weighing,
2) Calibration flask calculating the mean volume and
flask washed and dried, and then standard deviations and tolerance.
weighed empty flask filled with distilled Repeat these steps 3x
water to mark boundaries, kept cool to
III. RESULTS AND DISCUSSION
Labu Ukur

Suhu (C ) FK V Pindah V Ssebenarnya % Galat

20 1,0038 38,42 ml 50,19 76,5 %
20 1,0040 38,40 ml 50,20 76,49 %
20 1,0042 38,41 ml 50,21 76,49 %
20 1,0045 38,41 ml 50,22 76,48 %

Volume sebenarnya : massa air x Fk

I 50 ml x 1.0038 = 50.19
II 50 ml x 1.0040 = 50.20
III 50 ml x 1.0042 = 50.21
I 50 ml x 1.0045 = 50.22
V

Value correction = x 100%

I 38.42 / 50.19 x 100% = 76.5%

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II 38.40 / 50.20 x 100% = 76.49%

III 38.41 / 50.21 x 100% = 76.49%
IV 38.41 / 50.22 x 100% = 76.48%

So, calibrated measuring flask error because% > 50% and <100%

Gelas ukur

Suhu (C) FK V Pindah V Sebenarnya % Galat

20 1,0038 0,16 50,19 61 %
20 1,0040 0,65 50,20 65 %
20 1,0042 0,61 50,21 61 %
20 1,0045 0,59 50,22 59 %

volume of water actually = mass x Fk

I 50 ml x 1.0038 = 50.19
II 50 ml x 1.0040 = 50.20
III 50 ml x 1.0042 = 50.21
I 50 ml x 1.0045 = 50.22
V

Value correction = V Pindah – V Sebenarnya

I 50.80 - 50.19 x 100% = 61 %

II 50.85 - 50.20 x 100% = 65 %

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III 50.82 - 50.21 x 100% = 61 %

IV 50.81 - 50.22 x 100% = 59 %

So, calibrated measuring flask error because%> 50% and <100%

In the lab this time doing practical konvensional a measuring instrument.

work regarding the calibration praktikan
glassware. In general, the calibration
instrument calibration glass or glass
have an understanding as a series of
activities to compare the results of
measurements of a tool with the
appropriate standard tool
determining the amount of correction
and lack kepastiannya measurement
tool. Digunakan standard tools must
also be calibrated according to the
requirements of national
international standards. Calibrated
measuring devices will not continuously
apply the calibration period, because at
the time of use is still changing
specifications.
Calibration is done aiming to
achieve ketelusuran measurement. The
measurement results can be attributed or
traced back to a higher standard or
thorough (primary standard of national
and international), through rangkaian
perbandingan unbroken. Determine
deviations (storage) truth
Guaranteeing things measurement in
accordance with national and
international standards. Calibration is
done is also useful to maintain the
condition of measuring instruments and
measuring materials in keeping with the
for specifications. To support the quality
system is applied in various industries
in the laboratory and production
equipment owned. Can estimate the
difference (deviation) between the price
and indicated on the measuring instrument.
The calibration method that do must
conform to established procedures.
Volume measuring devices are
calibrated in this trial include the flask
and a measuring cup. Flask is a measure
of the volume containing a quantity of
liquid filled to the mark. Measuring cup
is a tool used to measure how banyak
substances used or a liquid solution that
will be used in accordance with the up
to the desired mark.
Calibration of measuring is done
value to adjust the output or indication of a

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volume measuring device to match the the alcohol quickly evaporates so as to

magnitude of the standards used within speed up drying.
specified accuracy. The principle of Terms of a calibration among the
measuring instrument calibration is reference standard that is capable of
performed by measuring the weight of searching to national or international
the volume of the volume of distilled standards and calibration methods are
water released by the measuring recognized nationally / internationally,
instrument volume. This weight is then personnel calibration trained as
compared with the specific gravity of evidenced by certification from an
water at a temperature of volume accredited laboratory, room calibration
measurement is done so that it can be conditioned such as temperature,
determined the value of accuracy. humidity, air pressure, air flow, which
For the experimental procedure in tool is calibrated in a state of well
a flask used is cleaned and dikeringkan functioning / not damaged.
(30 minutes, 100°C). flask from the From the calibration is done to get
oven allowed to stand for a minute, put the result, among others, object value
in ekrilator. Flask weighed measurement, correction values or
appropriately. flask filled with distilled deviations, the value of the uncertainty
water to the mark, the volume of 1g of of measurement (magnitude of the error
water at various temperatures into which may occur in the measurement),
account. Experiments conducted triplo. evaluated after the work is measured
In a measuring cup, the same and analyzed uncertainty properly
experiment was performed and almost taking into account all available
identical to the flask experiments. They resources in the fancy comparison
differed only in the measurement until method used and the amount of errors
the mark is 50 ml. To not use dryer that occur. The nature of other
drying apparatus for high temperature metrology, such as the calibration
ukur tool that expands and tools are not factor, the calibration curve. To get the
in standard conditions. To speed up the accurate mass measurements, booyancy
drying process tools can use blotting effect (due to the buoyancy of objects in
paper and enough alcohol and allowed the air stream). air at standard
to stand in the air. Due to the nature of conditions with the current situation is

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 Journal of Pharmacy Technology preparations Sterile Sriwijaya University
different weighed. Thus causing a
difference booyancy on these tools
(equilibrium level when the two objects
have the same mass)
to know how big the error of the
measurement results, it can be
calculated percent error. Where the
percent error is an absolute error of the
actual value multiplied by 100%. in a
measurement, the most important is the
extent to which the error is acceptable.
In a measuring cup and flask method of
calculation made the point where the
differences occur in the calculation for
the percent error. To flask% error or
errors obtained directly from the
volume switch and the actual volume by
means of the division. While measuring
cup minus.
The first test the calibration of the
flask, which had been cleaned and put
in the oven for the drying process. Do
as many as four times. Calibrating the
first flask 50 ml flask used for the mass
of the empty flask is 56.44 grams and a
mass of flask containing water into
55.635 grams. Mass of water obtained
from measurements of 45.53 grams.
Flask volume is 50.19 ml and the
percent average deviation obtained for
replication 4x range between 76% -
76.5% or a range between 0.076 to
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2017
0.07650. test results flask calibrated
instrument with good reason percent
error percent error or qualify. Where the
condition that the measuring instrument
can be calibrated by either having a rate
greater than 50% but not less than
100%.
In the second test for measuring
cup has been cleaned up and put in the
oven, dried, weighed in and filled the
void volume with distilled water or
distilled water. Similarly, the flask, the
first glass calibrating the measuring cup
used flask (50 ml measuring cup is done
as much as 4x). volume moved obtained
ranged between 50.80 ml - 50.82 ml.
Error percent gain ranging between
59% to 65% or 0.059 or 0.065. it can be
concluded from the results of a
calibrated measuring cup for percent
error of more than 50% and less than
100%.
Tool to be calibrated must be
absolutely clean and dry so that the
results obtained are accurate, precise,
and thorough. Measurements made a 3x
so that results are more accurate.
The results of the calibration
among other objects measuring values,
correction or deviation value and the
value of the uncertainty. Reference
standards are traceable to international
 Journal of Pharmacy Technology preparations Sterile Sriwijaya University 2017

standards or nasiional. The calibration correction factor is useful to know how

method that is recognized nationally or big an error in the calibration of
internationally, calibration trained equipment. The greater the deviation
personnel. then diminishing the accuracy of
Volumetric is a quantitative measurement.
analysis of the amount of a substance
that is sought by reacting a solution REFERENCES
volume of the substance with a solution
Akers, MJ 2010, Sterile Drug Products:
of a standard substance of known
Formulation,Packaging,Manufact
concentration. Key to the success of a
uring, Informa Healthcare, New
measuring instrument is the similarity
York, USA
of the volume obtained. Umtuk
Aulton, ME, 1994, Pharmaceutics, The
decimate the error in the measurement,
Science of Dosage
the tools to be used needs to be
Forms and Dug Delivery
calibrated beforehand. Calibration can
System,William and
be done by comparing two data using
Wilkins, Parkway PA, USA
different measuring devices

Felton , L. 2012, Remington: Essential

IV. CONCLUSION
of Pharmaceutics, Philladelphia,
Factors that contain calibration of
USA
measuring instruments are temperature,
Martin,A.1993.PhysicalChemical
kelmbaban, the density, etc. To
Prinsiplesin
determine a deviation of the measuring
PharmaceuticalScience, Waverly
instrument, must be known in advance
International, Maryland, USA
and the water mass correction factor. A

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