LAMPIRAN A

CONTOH PERHITUNGAN

1. Membuat larutan induk asam asetilsalisilat (1.000 ppm)

• Ditimbang asam asetilsalisilat 100 mg, ditambahkan aquades
sampai garis tanda dalam labu takar 100ml

• Di buat larutan dengan konsentrasi 500 ppm, 250 ppm, 125 ppm
dan 62,5 dari larutan induk

dipipet 5,0 ml larutan induk lalu di tambah aquades

sampai tanda pada labu takar 10,0 ml.

dipipet 2,5 ml larutan induk lalu di tambah aquades

sampai tanda pada labu takar 10,0 ml.

dipipet 1,25 ml larutan induk lalu di tambah aquades

sampai tanda pada labu takar 10,0 ml.

dipipet 0,625 ml larutan induk lalu di tambah aquades

sampai tanda pada labu takar 10,0 ml.

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• Cara yang sama seperti diatas pembuatan larutan induk atau pun
pengenceran untuk senyawa asam O-(3-
klorometilbenzoil)salisilat dan asam O-(4-
klorometilbenzoil)salisilat.
2. Menghitung LC50 Asam O-( 4-klorometilbenzoil)salisilat
% rata–rata
Konsentrasi Log konsentrasi kematian larva
Aedesaegypti Linn.
100 ppm 2,0 20
200 ppm 2,3010 43,33
300 ppm 2,4771 60
400 ppm 2,6021 83,33
500 ppm 2,6990 100

• Meregresi log konsentrasi versus % rata–rata kematian larva Aedes

aegypti Linn., didapatkan persamaan regresi yaitu,
Y = 112,29X – 209,95
• LC50 adalah konsentrasi yang dapat membunuh 50% hewan coba,
jadi untuk mendapatkan LC50, maka Y= 50
Y = 112,29X – 209,95
50 = 112,29X – 209,95
X = 2,3150
Anti log X = 206,54 ppm
Jadi LC50 asam O-(4-klorometilbenzoil)salisilat adalah 206,54 ppm.

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 LAMPIRAN B
ANALISIS PROBIT

1. AsamO-(3-klorometilbenzoil)salisilat
Parameter Estimates
95% Confidence
Interval
Std. Lower Upper
Parameter Estimate Error Z Sig. Bound Bound
PROBIT (a) Log_konsentrasi 27.892 8.713 3.20 .001 10.816 44.969
intercept -80.730 25.188 -3.205 .001 -105.918 -55.543

a PROBIT model:PROBIT(p) = intercept + BX

Cell Counts and Residuals

Number
Numb log_konse of Observed Expected Residua Probabil
er ntrasi Subjects Responses Responses l ity
PROBIT 1 2.860 10 2 1.709 .291 .171
2 2.875 10 3 2.953 .047 .295
3 2.889 10 4 4.436 -.436 .444
4 2.903 10 6 5.960 -.290 .596
5 2.929 10 9 8.356 .314 .836

Report
Rata-ratakematian
log_konsentrasi Mean N Std. Deviation
2.86 2.0000 1 .
2.88 3.0000 1 .
2.89 4.0000 1 .
2.90 5.6700 1 .
2.93 8.6700 1 .
Total 4.6680 5 2.61566

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 Confidence Limits
95% Confidence Limits for log_konsentrasi
Probability Estimate Lower Bound Upper Bound
PROBIT .010 2.811 2.683 2.843
.020 2.821 2.708 2.849
.030 2.827 2.724 2.854
.040 2.832 2.736 2.857
.050 2.835 2.745 2.859
.060 2.839 2.753 2.861
.070 2.841 2.761 2.863
.080 2.844 2.767 2.865
.090 2.846 2.773 2.866
.100 2.848 2.778 2.868
.150 2.857 2.800 2.874
.200 2.864 2.817 2.879
.250 2.870 2.831 2.884
.300 2.876 2.844 2.889
.350 2.881 2.855 2.894
.400 2.885 2.864 2.900
.450 2.890 2.872 2.906
.500 2.894 2.879 2.914
.550 2.899 2.885 2.922
.600 2.903 2.890 2.932
.650 2.908 2.895 2.943
.700 2.913 2.899 2.954
.750 2.919 2.903 2.967
.800 2.925 2.908 2.982
.850 2.932 2.913 2.999
.900 2.940 2.919 3.021
.910 2.942 2.920 3.027
.920 2.945 2.922 3.033
.930 2.947 2.923 3.039
.940 2.950 2.925 3.046
.950 2.953 2.927 3.054
.960 2.957 2.930 3.064
.970 2.962 2.933 3.076
.980 2.968 2.937 3.092
.990 2.978 2.943 3.117

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 2. Asam O-(4-klorometilbenzoil)salisilat
Parameter Estimates
95% Confidence
Interval
Std. Lower Upper
Parameter Estimate Error Z Sig. Bound Bound
PROBIT (a) Log_konsentrasi 3.453 .904 3.819 .000 1.681 5.225
intercept -7.984 2.171 -3.677 .000 -10.155 -5.813

a PROBIT model:PROBIT(p) = intercept + BX

Cell Counts and Residuals

Number
log_ of Observed Expected
Number konsentrasi Subjects Responses Responses Residual Probability
PROBI 1
2.000 10 2 1.405 .595 .140
T
2 2.301 10 4 4.845 -.515 .484
3 2.477 10 6 7.154 -1.154 .715
4 2.602 10 8 8.415 -.085 .842
5 2.699 10 10 9.091 .909 .909

Report
rata_ratakematian
log_konsentrasi Mean N Std. Deviation
2.00 2.0000 1 .
2.30 4.3300 1 .
2.48 6.0000 1 .
2.60 8.3300 1 .
2.70 10.0000 1 .
Total 6.1320 5 3.16744

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 Confidence Limits
Confidence Limits

95% Confidence Limits for log_konsentrasi

Probability Estimate Lower Bound Upper Bound
PROBIT .010 1.639 .828 1.911
.020 1.717 .988 1.965
.030 1.768 1.090 2.000
.040 1.805 1.166 2.026
.050 1.836 1.228 2.047
.060 1.862 1.280 2.065
.070 1.885 1.326 2.082
.080 1.905 1.367 2.096
.090 1.924 1.405 2.109
.100 1.941 1.439 2.122
.150 2.012 1.580 2.173
.200 2.069 1.691 2.215
.250 2.117 1.785 2.253
.300 2.160 1.868 2.288
.350 2.201 1.943 2.322
.400 2.239 2.013 2.356
.450 2.276 2.077 2.392
.500 2.312 2.138 2.430
.550 2.349 2.195 2.472
.600 2.386 2.248 2.519
.650 2.424 2.298 2.573
.700 2.464 2.346 2.635
.750 2.508 2.391 2.707
.800 2.556 2.437 2.793
.850 2.612 2.486 2.897
.900 2.683 2.543 3.033
.910 2.701 2.556 3.066
.920 2.719 2.571 3.103
.930 2.740 2.586 3.143
.940 2.763 2.603 3.188
.950 2.789 2.622 3.240
.960 2.819 2.645 3.300
.970 2.857 2.672 3.376
.980 2.907 2.707 3.476
.990 2.986 2.763 3.635

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 3. AsamAsetilsalisilat
Parameter Estimates
95%
Confidenc
e Interval
Std. Lower Upper
Parameter Estimate Error Z Sig. Bound Bound
PROBIT (a) Log_konsentrasi 8.788 3.786 2.321 .020 1.367 16.210
intercept -23.915 10.482 -2.282 .023 -34.396 -13.433
a PROBIT model: PROBIT(p) = Intercept + BX

Cell Counts and Residuals

Number
log_ of Observed Expected
Number konsentrasi Subjects Responses Responses Residual Probability
PROBI 1
2.699 10 4 4.226 .104 .423
T
2 2.740 10 5 5.670 -.340 .567
3 2.778 10 7 6.918 .412 .692
4 2.813 10 8 7.898 -.228 .790
5 2.845 10 9 8.619 .051 .862

Report
rata_ratakematian
log_konsentrasi Mean N Std. Deviation
2.70 4.3300 1 .
2.74 5.3300 1 .
2.78 7.3300 1 .
2.81 7.6700 1 .
2.85 8.6700 1 .
Total 6.6660 5 1.78232

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 Confidence Limits

95% Confidence Limits for log_konsentrasi

Probability Estimate Lower Bound Upper Bound
PROBIT .010 2.456 .763 2.602
.020 2.488 .962 2.620
.030 2.507 1.089 2.631
.040 2.522 1.183 2.639
.050 2.534 1.261 2.646
.060 2.544 1.326 2.651
.070 2.553 1.384 2.656
.080 2.561 1.436 2.661
.090 2.569 1.482 2.665
.100 2.575 1.526 2.669
.150 2.603 1.704 2.685
.200 2.625 1.846 2.698
.250 2.644 1.967 2.709
.300 2.662 2.075 2.720
.350 2.677 2.175 2.730
.400 2.692 2.270 2.741
.450 2.707 2.360 2.752
.500 2.721 2.447 2.764
.550 2.736 2.532 2.779
.600 2.750 2.611 2.801
.650 2.765 2.679 2.838
.700 2.781 2.725 2.902
.750 2.798 2.754 2.993
.800 2.817 2.774 3.105
.850 2.839 2.792 3.242
.900 2.867 2.811 3.417
.910 2.874 2.815 3.460
.920 2.881 2.820 3.506
.930 2.889 2.825 3.557
.940 2.898 2.830 3.614
.950 2.908 2.836 3.680
.960 2.920 2.844 3.757
.970 2.935 2.852 3.851
.980 2.955 2.863 3.977
.990 2.986 2.881 4.176

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