LAMPIRAN A

CONTOH PERHITUNGAN PEMBUATAN LARUTAN UJI

Membuat larutan induk asam asetilsalisilat (10000 ppm)

• Ditimbang asetosal 50 mg, ditambahkan DMSO sampai garis
tanda dalam labu takar 5ml
50 mg X 1000
 10000 ppm
5 ml
• Dibuat larutan dengan konsentrasi 1000 ppm, 500 ppm, 250
ppm, 125 ppm dan 62,5 dari larutan induk
µ
- X 10000 ppm  1000 ppm
 

dipipet 500 µl larutan induk lalu ditambah aquades

sampai tanda pada vial 5,0 ml.
• Cara yang sama seperti di atas pembuatan larutan induk atau
pun pengenceran untuk senyawa asam O-(3-
klorometilbenzoil)salisilat dan asam O-(4-
klorometilbenzoil)salisilat.

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 LAMPIRAN B
ANALISIS PROBIT

1. Asam asetilsalisilat

Parameter Estimates
95% Confidence Interval
Std.
Parameter Estimate Error Z Sig. Lower Bound Upper Bound
a 8.798 1.223 7.193 .000 6.401 11.195
PROBIT a_conc

Intercept -21.182 2.942 -7.199 .000 -24.124 -18.240

a. PROBIT model: PROBIT(p) = Intercept + BX

Cell Counts and Residuals

Number of Observed Expected
No a_conc Subjects Responses Responses Residual Probability
PROBIT
1 2.176 30 2 .625 1.375 .021
2 2.301 30 5 5.229 -.229 .174
3 2.398 30 10 13.988 -3.988 .466
4 2.477 30 20 21.891 -1.891 .730
5 2.544 30 30 26.553 3.447 .885

Report
Rata-ratakematian
log_konsentrasi Mean N Std. Deviation
2.18 .6700 1 .
2.30 1.6700 1 .
2.40 3.3300 1 .
2.48 6.6700 1 .
2.54 10.0000 1 .
Total 4.4680 5 3.84059

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 Confidence Limits

95% Confidence Limits for 95% Confidence Limits for

a_conc log(a_conc)a

Estimate Lower Upper Estimate Lower Upper

Probability Bound Bound Bound Bound

PROBIT 0.01 139.042 12.844 188.052 2.143 1.109 2.274

0.02 149.325 18.154 196.604 2.174 1.259 2.294

0.03 156.239 22.595 202.371 2.194 1.354 2.306

0.04 161.651 26.626 206.915 2.209 1.425 2.316

0.05 166.191 30.419 210.763 2.221 1.483 2.324

0.06 170.156 34.059 214.159 2.231 1.532 2.331

0.07 173.710 37.598 217.241 2.240 1.575 2.337

0.08 176.955 41.068 220.091 2.248 1.613 2.343

0.09 179.959 44.490 222.768 2.255 1.648 2.348

0.1 182.769 47.881 225.309 2.262 1.680 2.353

0.15 194.878 64.714 236.830 2.290 1.811 2.374

0.2 205.071 81.821 247.606 2.312 1.913 2.394

0.25 214.241 99.504 258.681 2.331 1.998 2.413

0.3 222.824 117.806 270.900 2.348 2.071 2.433

0.35 231.084 136.556 285.224 2.364 2.135 2.455

0.4 239.205 155.347 302.897 2.379 2.191 2.481

0.45 247.334 173.559 325.526 2.393 2.239 2.513

0.5 255.603 190.517 355.037 2.408 2.280 2.550

0.55 264.149 205.766 393.558 2.422 2.313 2.595

0.6 273.125 219.249 443.484 2.436 2.341 2.647

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0.65 282.724 231.258 507.950 2.451 2.364 2.706

0.7 293.205 242.257 591.786 2.467 2.384 2.772

0.75 304.951 252.767 703.220 2.484 2.403 2.847

0.8 318.586 263.364 857.489 2.503 2.421 2.933

0.85 335.251 274.801 1086.330 2.525 2.439 3.036

0.9 357.463 288.410 1470.478 2.553 2.460 3.167

0.91 363.044 291.623 1582.992 2.560 2.465 3.199

0.92 369.207 295.092 1715.354 2.567 2.470 3.234

0.93 376.104 298.890 1874.114 2.575 2.476 3.273

0.94 383.960 303.116 2069.349 2.584 2.482 3.316

0.95 393.119 307.926 2317.583 2.595 2.488 3.365

0.96 404.160 313.577 2648.362 2.607 2.496 3.423

0.97 418.159 320.540 3121.623 2.621 2.506 3.494

0.98 437.523 329.854 3886.306 2.641 2.518 3.590

0.99 469.878 344.749 5494.672 2.672 2.538 3.740

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2. AsamO-(3-klorometilbenzoil)salisilat
Parameter Estimates
95% Confidence Interval
Std.
Parameter Estimate Error Z Sig. Lower Bound Upper Bound
a
PROBIT a_conc 10.965 1.655 6.626 .000 7.721 14.208
Intercept -28.539 4.306 -6.628 .000 -32.845 -24.233
a. PROBIT model: PROBIT(p) = Intercept + BX

Cell Counts and Residuals

Number of Observed Expected
No a_conc Subjects Responses Responses Residual Probability
PROBIT
1 2.477 30 3 2.521 .479 .084
2 2.544 30 7 7.790 -.790 .260
3 2.602 30 15 14.899 .101 .497
4 2.653 30 21 21.290 -.290 .710
5 2.699 30 26 25.622 .378 .854

Report
Rata-ratakematian
log_konsentrasi Mean N Std. Deviation
2.48 1.0000 1 .
2.54 2.3300 1 .
2.60 5.0000 1 .
2.65 7.0000 1 .
2.70 8.6700 1 .
Total 4.8000 5 3.17796

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 Confidence Limits

95% Confidence Limits for a_conc 95% Confidence Limits for log(a_conc)a

Probability Estimate Lower Bound Upper Bound Estimate Lower Bound Upper Bound

PROBIT 0.01 245.845 198.968 276.776 2.391 2.299 2.442

0.02 260.330 215.607 289.561 2.416 2.334 2.462

0.03 269.959 226.848 298.017 2.431 2.356 2.474

0.04 277.437 235.668 304.567 2.443 2.372 2.484

0.05 283.672 243.079 310.023 2.453 2.386 2.491

0.06 289.089 249.555 314.761 2.461 2.397 2.498

0.07 293.924 255.363 318.990 2.468 2.407 2.504

0.08 298.322 260.666 322.840 2.475 2.416 2.509

0.09 302.379 265.574 326.394 2.481 2.424 2.514

0.1 306.162 270.163 329.714 2.486 2.432 2.518

0.15 322.334 289.879 343.989 2.508 2.462 2.537

0.2 335.795 306.330 356.051 2.526 2.486 2.552

0.25 347.790 320.925 367.036 2.541 2.506 2.565

0.3 358.926 334.319 377.531 2.555 2.524 2.577

0.35 369.564 346.871 387.924 2.568 2.540 2.589

0.4 379.950 358.795 398.520 2.580 2.555 2.600

0.45 390.276 370.233 409.586 2.591 2.568 2.612

0.5 400.712 381.305 421.373 2.603 2.581 2.625

0.55 411.427 392.137 434.131 2.614 2.593 2.638

0.6 422.609 402.890 448.122 2.626 2.605 2.651

0.65 434.485 413.772 463.663 2.638 2.617 2.666

0.7 447.362 425.057 481.191 2.651 2.628 2.682

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0.75 461.688 437.122 501.377 2.664 2.641 2.700

0.8 478.180 450.534 525.350 2.680 2.654 2.720

0.85 498.148 466.270 555.240 2.697 2.669 2.744

0.9 524.462 486.404 595.826 2.720 2.687 2.775

0.91 531.024 491.341 606.133 2.725 2.691 2.783

0.92 538.245 496.741 617.558 2.731 2.696 2.791

0.93 546.298 502.726 630.395 2.737 2.701 2.800

0.94 555.435 509.471 645.078 2.745 2.707 2.810

0.95 566.042 517.248 662.278 2.753 2.714 2.821

0.96 578.763 526.502 683.115 2.763 2.721 2.834

0.97 594.795 538.064 709.689 2.774 2.731 2.851

0.98 616.796 553.765 746.706 2.790 2.743 2.873

0.99 653.135 579.330 809.173 2.815 2.763 2.908

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3. AsamO-(4-klorometilbenzoil)salisilat

Parameter Estimates
95% Confidence Interval
Std.
Parameter Estimate Error Z Sig. Lower Bound Upper Bound
a 5.367 1.223 4.389 .000 2.970 7.764
PROBIT a_conc
Intercept -13.534 3.101 -4.364 .000 -16.635 -10.433
a. PROBIT model: PROBIT(p) = Intercept + BX

Cell Counts and Residuals

Number of Observed Expected
No a_conc Subjects Responses Responses Residual Probability
PROBIT
1 2.398 30 7 7.592 -.592 .253
2 2.477 30 13 12.156 .844 .405
3 2.544 30 17 16.425 .575 .548
4 2.602 30 19 19.999 -.999 .667
5 2.653 30 23 22.789 .211 .760

Report
Rata-ratakematian
log_konsentrasi Mean N Std. Deviation
2.40 2.3300 1 .
2.48 4.3300 1 .
2.54 5.6700 1 .
2.60 6.3300 1 .
2.65 7.6700 1 .
Total 5.2660 5 2.03536

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 Confidence Limits
95% Confidence Limits for a_conc 95% Confidence Limits for log(a_conc)a
Probability Estimate Lower Bound Upper Bound Estimate Lower Bound Upper Bound
PROBIT 0.01 122.561 53.019 169.787 2.088 1.724 2.230
0.02 137.767 65.425 184.287 2.139 1.816 2.265
0.03 148.379 74.749 194.153 2.171 1.874 2.288
0.04 156.897 82.621 201.942 2.196 1.917 2.305
0.05 164.186 89.625 208.524 2.215 1.952 2.319
0.06 170.656 96.045 214.309 2.232 1.982 2.331
0.07 176.538 102.046 219.527 2.247 2.009 2.341
0.08 181.976 107.731 224.319 2.260 2.032 2.351
0.09 187.068 113.170 228.779 2.272 2.054 2.359
0.1 191.880 118.413 232.974 2.283 2.073 2.367
0.15 213.159 142.742 251.339 2.329 2.155 2.400
0.2 231.741 165.417 267.251 2.365 2.219 2.427
0.25 248.969 187.483 282.058 2.396 2.273 2.450
0.3 265.529 209.456 296.535 2.424 2.321 2.472
0.35 281.856 231.593 311.306 2.450 2.365 2.493
0.4 298.276 253.936 327.057 2.475 2.405 2.515
0.45 315.072 276.286 344.690 2.498 2.441 2.537
0.5 332.525 298.179 365.433 2.522 2.474 2.563
0.55 350.945 319.082 390.733 2.545 2.504 2.592
0.6 370.708 338.828 421.932 2.569 2.530 2.625
0.65 392.304 357.851 460.208 2.594 2.554 2.663
0.7 416.426 376.979 507.087 2.620 2.576 2.705
0.75 444.124 397.225 565.238 2.648 2.599 2.752
0.8 477.140 419.870 639.668 2.679 2.623 2.806
0.85 518.735 446.923 740.501 2.715 2.650 2.870
0.9 576.261 482.525 891.956 2.761 2.684 2.950
0.91 591.086 491.438 933.157 2.772 2.691 2.970

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0.92 607.624 501.273 980.143 2.784 2.700 2.991
0.93 626.343 512.277 1034.618 2.797 2.710 3.015
0.94 647.932 524.811 1099.130 2.812 2.720 3.041
0.95 673.463 539.434 1177.733 2.828 2.732 3.071
0.96 704.748 557.080 1277.420 2.848 2.746 3.106
0.97 745.207 579.496 1411.783 2.872 2.763 3.150
0.98 802.610 610.593 1612.795 2.905 2.786 3.208
0.99 902.191 662.834 1989.872 2.955 2.821 3.299

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