DAFTAR ISI

HALAMAN JUDUL...........................................................................................i
LEMBAR PENGESAHAN................................................................................ii
KATA PENGANTAR.........................................................................................iii
DAFTAR ISI.......................................................................................................iv

PRAKTIKUM I (STERILISASI PERALATAN DAN PEMBUATAN

MEDIA)
BAB I PENDAHULUAN..............................................................................1
1.1 Latar Belakang............................................................................................1
1.2 Tujuan.........................................................................................................1
BAB II TINJAUAN PUSTAKA.....................................................................2
BAB III METODE KERJA.............................................................................8
3.1 Waktu dan Tempat.......................................................................................8
3.2 Alat dan Bahan............................................................................................8
3.2.1 Alat..................................................................................................8
3.2.2 Bahan...............................................................................................8
3.3 Cara Kerja...................................................................................................9
3.3.1 Cara Kerja Sterilisasi pada Cawan Petri.........................................9
3.3.2 Cara Kerja Pembuatan Media NA...................................................9
3.3.3 Cara Kerja Pembuatan Media PDA................................................9
3.3.4 Cara Kerja Pembuatan NaCl 0,9%..................................................9
BAB IV HASIL DAN PEMBAHASAN.........................................................11
4.1 Hasil Pengamatan.......................................................................................11
4.1.1 Peralatan.........................................................................................11
4.1.2 Sterilisasi........................................................................................12
4.1.3 Pembuatan Media...........................................................................13
4.2 Pembahasan................................................................................................14
BAB V PENUTUP..........................................................................................16
5.1 Kesimpulan................................................................................................16
5.2 Saran...........................................................................................................16
DAFTAR PUSTAKA

PRAKTIKUM II (ISOLASI DAN IDENTIFIKASI DASAR KOLONI

MIKROBA)
BAB I PENDAHULUAN.............................................................................18
1.1 Latar Belakang...........................................................................................18
1.2 Tujuan........................................................................................................18
BAB II TINJAUAN PUSTAKA....................................................................19
BAB III METODE KERJA..............................................................................
3.1 Waktu dan Tempat......................................................................................23
3.2 Alat dan Bahan...........................................................................................23
3.2.1 Alat.................................................................................................23
3.2.2 Bahan..............................................................................................23
3.3 Cara Kerja..................................................................................................24
3.3.1 Cara Kerja NA................................................................................24
3.3.2 Cara Kerja PDA.............................................................................25
BAB IV HASIL DAN PEMBAHASAN.........................................................26
4.1 Hasil dan Pengamatan................................................................................26
4.1.1 Tabel Hasil Pengamatan.................................................................26
4.2 Pembahasan................................................................................................27
BAB V PENUTUP..........................................................................................30
5.1 Kesimpulan................................................................................................30
5.2 Saran...........................................................................................................31
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM III (PEMBUATAN BIAKAN MURNI)

BAB I PENDAHULUAN.............................................................................33
1.1 Latar Belakang...........................................................................................33
1.2 Tujuan Praktikum.......................................................................................33
BAB II TINJAUAN PUSTAKA....................................................................34
BAB III METODE KERJA............................................................................38
3.1 Waktu dan Tempat......................................................................................38
3.2 Alat dan Bahan...........................................................................................38
3.2.1 Alat.................................................................................................38
3.2.2 Bahan..............................................................................................39
3.3 Cara Kerja..................................................................................................39
3.3.1 Cara Kerja Metode Streak (Gores).................................................39
3.3.2 Cara Kerja Metode Spread (Sebar)................................................39
BAB IV HASIL DAN PEMBAHASAN.........................................................41
4.1 Hasil dan Pengamatan................................................................................41
4.1.1 Tabel Hasil Pengamatan.................................................................41
4.2 Pembahasan................................................................................................42
BAB V PENUTUP..........................................................................................45
5.1 Kesimpulan................................................................................................45
5.2 Saran...........................................................................................................45
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM IV (PEWARNAAN DAN CARA-CARA PEWARNAAN)

BAB I PENDAHULUAN.............................................................................47
1.1 Latar Belakang...........................................................................................47
1.2 Tujuan Praktikum.......................................................................................48
BAB II TINJAUAN PUSTAKA....................................................................49
BAB III METODE KERJA............................................................................56
3.1 Waktu dan Tempat......................................................................................56
3.2 Alat dan Bahan...........................................................................................56
3.2.1 Alat.................................................................................................56
3.2.2 Bahan..............................................................................................56
3.3 Prosedur Kerja............................................................................................57
3.3.1 Prosedur Kerja Pewarnaan Sederhana............................................57
3.3.2 Prosedur Kerja Pewarnaan Negatif................................................57
3.3.3 Prosedur Kerja Pewarnaan Gram...................................................58
3.3.4 Prosedur Kerja Pewarnaan Spora...................................................58
BAB IV HASIL DAN PEMBAHASAN.........................................................60
4.1 Hasil Pengamatan.......................................................................................60
4.1.1 Tabel Hasil Pengamatan Pengamatan Pewarnaan dan Cara-Cara
Pewarnaan......................................................................................60
4.2 Pembahasan................................................................................................61
BAB V PENUTUP..........................................................................................63
5.1 Kesimpulan................................................................................................63
5.2 Saran...........................................................................................................63
DAFTAR PUSTAKA

PRAKTIKUM V (PENGAMATAN JAMUR MIKROSKOPIS)

BAB I PENDAHULUAN.............................................................................65
1.1 Latar Belakang...........................................................................................65
1.2 Tujuan Praktikum.......................................................................................65
BAB II TINJAUAN PUSTAKA....................................................................66
BAB III METODE KERJA............................................................................70
3.1 Waktu dan Tempat......................................................................................70
3.2 Alat dan Bahan...........................................................................................70
3.2.1 Alat.................................................................................................70
3.2.2 Bahan..............................................................................................70
3.3 Prosedur Kerja............................................................................................71
BAB IV HASIL DAN PEMBAHASAN.........................................................72
4.1 Hasil Pengamatan.......................................................................................72
4.1.1 Tabel Hasil Pengamatan.................................................................72
4.2 Pembahasan................................................................................................72
BAB V PENUTUP..........................................................................................77
5.1 Kesimpulan................................................................................................77
5.2 Saran...........................................................................................................77
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM VI (PENGUKURAN PERTUMBUHAN

MIKROORGANISME)
BAB I PENDAHULUAN.............................................................................79
1.1 Latar Belakang...........................................................................................79
1.2 Tujuan Praktikum.......................................................................................79
BAB II TINJAUAN PUSTAKA....................................................................81
BAB III METODE KERJA............................................................................88
3.1 Waktu dan Tempat......................................................................................88
3.2 Alat dan Bahan...........................................................................................88
3.2.1 Alat.................................................................................................88
 3.2.2 Bahan..............................................................................................89
3.3 Cara Kerja..................................................................................................89
3.3.1 Pertumbuhan Mikroorganisme pada Durasi 0..............................89
3.3.2 Pertumbuhan Mikroorganisme pada Durasi 15............................90
3.3.3 Pertumbuhan Mikroorganisme pada Durasi 30............................90
BAB IV HASIL DAN PEMBAHASAN.........................................................91
4.1 Hasil Pengamatan.......................................................................................91
4.1.1 Tabel Hasil Pengamatan Pertumbuhan Mikroorganisme...............91
4.2 Perhitungan................................................................................................92
4.2.1.........................................................................Perhitungan Durasi 0
........................................................................................................92
4.2.2.........................................................................Perhitungan Durasi 15
........................................................................................................92
4.2.3.......................................................................Perhitungan Durasi 30
........................................................................................................92
4.3 Pembahasan................................................................................................92
BAB V PENUTUP..........................................................................................96
5.1 Kesimpulan................................................................................................96
5.2 Saran...........................................................................................................96
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM VII (PENGONTROLAN MIKROORGANISME)

BAB I PENDAHULUAN.............................................................................98
1.1 Latar Belakang...........................................................................................98
1.2 Tujuan Praktikum.......................................................................................99
BAB II TINJAUAN PUSTAKA...................................................................100
BAB III METODE KERJA...........................................................................104
3.1 Waktu dan Tempat.....................................................................................104
3.2 Alat dan Bahan..........................................................................................104
3.2.1 Alat................................................................................................104
3.2.2 Bahan.............................................................................................105
3.3 Cara Kerja.................................................................................................105
3.3.1 Cara kerja pada ekstrak ................................................................105
3.3.2 Cara kerja pada desinfektan..........................................................106
BAB IV HASIL DAN PEMBAHASAN........................................................107
4.1 Hasil Pengamatan......................................................................................107
4.1.1 Tabel Indeks Antimikrobial...........................................................107
4.2 Pembahasan...............................................................................................107
BAB V PENUTUP.........................................................................................111
5.1 Kesimpulan...............................................................................................111
5.2 Saran..........................................................................................................112
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM VII (ANALISA KUALITAS AIR DENGAN METODE MPN)

BAB I PENDAHULUAN.............................................................................114
1.1 Latar Belakang..........................................................................................114
1.2 Tujuan Praktikum......................................................................................115
BAB II TINJAUAN PUSTAKA...................................................................116
BAB III METODE KERJA...........................................................................120
3.1 Waktu dan Tempat.....................................................................................120
3.2 Alat dan Bahan..........................................................................................120
3.2.1 Alat................................................................................................120
3.2.2 Bahan.............................................................................................121
3.3 Cara Kerja.................................................................................................121
3.3.1 Pembuatan Media LB....................................................................121
3.3.2 Pembuatan Media BGLBB............................................................122
3.3.3 Pembuatan NaCl............................................................................122
3.3.4 Uji Pendugaan...............................................................................122
3.3.5 Uji Penegas....................................................................................123
3.3.6 Uji Pelengkap................................................................................124
BAB IV HASIL DAN PEMBAHASAN........................................................126
4.1 Hasil Pengamatan......................................................................................126
4.1.1 Tabel Hasil Pengamatan Analisis Terhadap Es Teh Dengan
Metode MPN.................................................................................126
4.2 Perhitungan MPN......................................................................................126
4.2.1 Media LB......................................................................................126
4.2.2 Media BGLBB..............................................................................127
4.2.3 Media EMBA................................................................................127
4.3 Pembahasan..................................................................................................127
BAB V PENUTUP.........................................................................................130
5.1 Kesimpulan...............................................................................................130
5.2 Saran..........................................................................................................130
DAFTAR PUSTAKA
LAMPIRAN