LAPORAN RESMI

PRAKTIKUM MIKROBIOLOGI FARMASI

DISUSUN OLEH :
KELOMPOK 1
SHIFT A-2

NAMA NIM

ANDY WAHYUDI 723901S.11.006

FEDRI BAYSAR 723901S.11.027
GLADYS AUSTIN 723901S.11.031
GUSTIA VALENTINA 723901S.11.033
LIBIYAH 723901S.11.042

LABORATORIUM MIKROBIOLOGI DAN BIOTEKNOLOGI

FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS MULAWARMAN
SAMARINDA
2012
LEMBAR PENGESAHAN
 LAPORAN RESMI
PRAKTIKUM BIOLOGI DAN MIKROBIOLOGI

Laporan praktikum Mikrobiologi ini telah diperiksa tanggal 14 bulan Mei

tahun 2012, dan telah memenuhi syarat.

Disetujui Oleh :

Asisten I Asisten II

Dina Septiana Annizah Saleng

0907025013 0907025074

Laboran,

Eko Kusumawati, S.Si, MP

Mengetahui,
Kepala Laboratorium

Ir. Samsurianto, M.Si

NIP. 1962118 198903 1 001

KATA PENGANTAR

Assalamualaikum wr.wb

Puji syukur kehadirat Allah SWT karena dengan berkat dan hidayahnya
kami dapat menyelesaikan laporan resmi Mikrobiologi ini dengan tepat pada
waktunya. Laporan resmi Mikrobiologi ini merupakan laporan yang telah direvisi
dari laporan mingguan yang telah kami susu.

Di dalam laporan resmi Mikrobiologi ini dimuat berbagai materi-materi

mikrobiologi selama proses praktikum berlangsung dilengkapi dengan lembar cover
depan buku yang menjadi sumber acuan sehingga memudahkan nantinya jika ingin
mencari mengenai materi tersebut lebih lanjut.

Dengan adanya laporan resmi ini diharapkan dapat membantu para

praktikan-praktikan disemester bawah untuk dapat membuat laporan praktikum yang
lebih baik lagi sehingga dapat dijadikan salah satu bahan referensi selain buku-buku
utama nantinya.

Dalam hal penyusunan dan penulisan pun tidak dipungkiri dari berbagai
kesalahan dan kekurangan. Oleh sebab itu, kami selaku tim penyusun meminta maaf
sekiranya dalam laporan kami ini masih terdapat banyak kesalahan.

Penulis

Tim Penyusun

DAFTAR ISI

HALAMAN JUDUL...........................................................................................i
LEMBAR PENGESAHAN................................................................................ii
KATA PENGANTAR.........................................................................................iii
DAFTAR ISI.......................................................................................................iv

PRAKTIKUM I (STERILISASI PERALATAN DAN PEMBUATAN MEDIA)

BAB I PENDAHULUAN..............................................................................1
1.1 Latar Belakang............................................................................................1
1.2 Tujuan Praktikum........................................................................................3
BAB II TINJAUAN PUSTAKA.....................................................................4
BAB III METODE KERJA............................................................................10
3.1 Waktu dan Tempat......................................................................................10
3.2 Alat dan Bahan...........................................................................................10
3.2.1 Alat.................................................................................................10
3.2.2 Bahan..............................................................................................10
3.3 Cara Kerja..................................................................................................11
3.3.1 Sterilisasi Pada Cawan Petri...........................................................11
3.3.2 Penggunaan Autoclave...................................................................11
3.3.3 Pembuatan Media PDA..................................................................12
BAB IV HASIL DAN PEMBAHASAN.........................................................13
4.1 Hasil Pengamatan.......................................................................................13
4.1.1 Tabel Hasil Pengamatan Peralatan.................................................13
4.1.2 Tabel Hasil Pengamatan Sterilisasi................................................14
4.1.3 Tabel Hasil Pengamatan Pembuatan Media...................................14
4.2 Pembahasan................................................................................................15
BAB V PENUTUP..........................................................................................19
5.1 Kesimpulan................................................................................................19
5.2 Saran...........................................................................................................19
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM II (ISOLASI DAN MORFOLOGI KOLONI MOKROBA )

BAB I PENDAHULUAN.............................................................................21
1.1 Latar Belakang...........................................................................................21
1.2 Tujuan Praktikum.......................................................................................22
BAB II TINJAUAN PUSTAKA....................................................................23
BAB III METODE KERJA............................................................................28
3.1 Waktu dan Tempat......................................................................................28
3.2 Alat dan Bahan...........................................................................................28
3.2.1 Alat.................................................................................................28
3.2.2 Bahan..............................................................................................28
3.3 Cara Kerja..................................................................................................29
3.3.1 Cara Kerja Untuk Media NA .........................................................29
3.3.2 Cara Kerja Untuk Media PDA ......................................................30
BAB IV HASIL DAN PEMBAHASAN.........................................................31
4.1 Hasil Pengamatan.......................................................................................31
4.1.1 Tabel Hasil Pengamatan Terhadap Kue Talam...............................31
4.2 Pembahasan................................................................................................32
BAB V PENUTUP..........................................................................................37
5.1 Kesimpulan................................................................................................37
5.2 Saran...........................................................................................................37
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM III (PEMBUATAN BIAKAN MURNI)

BAB I PENDAHULUAN.............................................................................39
4
1.1 Latar Belakang...........................................................................................39
1.2 Tujuan Praktikum.......................................................................................39
BAB II TINJAUAN PUSTAKA....................................................................41
BAB III METODE KERJA............................................................................45
3.1 Waktu dan Tempat......................................................................................45
3.2 Alat dan Bahan...........................................................................................45
3.2.1 Alat.................................................................................................45
3.2.2 Bahan..............................................................................................45
3.3 Cara Kerja..................................................................................................46
3.3.1 Pembuatan Biakan Murni Dengan Metode Streak Plate................46
3.3.2 Pembuatan Biakan Murni Dengan Metode Spread Plate...............47
BAB IV HASIL DAN PEMBAHASAN.........................................................48
4.1 Hasil Pengamatan.......................................................................................48
4.1.1 Tabel Hasil Pengamatan Pembuatan Biakan Murni.......................48
4.2 Pembahasan................................................................................................48
BAB V PENUTUP..........................................................................................52
5.1 Kesimpulan................................................................................................52
5.2 Saran...........................................................................................................52
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM IV (PEWARNAAN DAN CARA-CARA PEWARNAAN)

BAB I PENDAHULUAN.............................................................................54
1.1 Latar Belakang...........................................................................................54
1.2 Tujuan Praktikum.......................................................................................54
BAB II TINJAUAN PUSTAKA....................................................................55
BAB III METODE KERJA............................................................................62
3.1 Waktu dan Tempat......................................................................................62
3.2 Alat dan Bahan...........................................................................................62
3.2.1 Alat.................................................................................................62
3.2.2 Bahan..............................................................................................62
3.3 Cara Kerja..................................................................................................63
3.3.1 Pewarnaan Sederhana.....................................................................63
3.3.2 Pewarnaan Negatif.........................................................................63
3.3.3 Pewarnaan Gram............................................................................64
3.3.4 Pewarnaan Spora............................................................................64
BAB IV HASIL DAN PEMBAHASAN.........................................................66
4.1 Hasil Pengamatan.......................................................................................66
4.1.1 Tabel Hasil Pengamatan Pewarnaan Pada Bakteri.........................66
4.2 Pembahasan................................................................................................67
BAB V PENUTUP..........................................................................................74
5.1 Kesimpulan................................................................................................74
5.2 Saran...........................................................................................................74
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM V (PENGAMATAN JAMUR MIKROSKOPIS)

BAB I PENDAHULUAN.............................................................................76
1.1 Latar Belakang...........................................................................................76
1.2 Tujuan Praktikum.......................................................................................76
BAB II TINJAUAN PUSTAKA....................................................................77
BAB III METODE KERJA............................................................................81
3.1 Waktu dan Tempat......................................................................................81
3.2 Alat dan Bahan...........................................................................................81
3.2.1 Alat.................................................................................................81
3.2.2 Bahan..............................................................................................81
3.3 Cara Kerja..................................................................................................82
BAB IV HASIL DAN PEMBAHASAN.........................................................83
4.1 Hasil Pengamatan.......................................................................................83
4.1.1 Tabel Hasil Pengamatan.................................................................83
4.2 Pembahasan................................................................................................83
BAB V PENUTUP..........................................................................................88
5.1 Kesimpulan................................................................................................88
5.2 Saran...........................................................................................................88
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM VI (PENGUKURAN PERTUMBUHAN

MIKROORGANISME)
BAB I PENDAHULUAN.............................................................................90
1.1 Latar Belakang...........................................................................................90
1.2 Tujuan Praktikum.......................................................................................91
BAB II TINJAUAN PUSTAKA....................................................................92
BAB III METODE KERJA............................................................................97
3.1 Waktu dan Tempat......................................................................................97
3.2 Alat dan Bahan...........................................................................................97
3.2.1 Alat.................................................................................................97
3.2.2 Bahan..............................................................................................98
3.3 Cara Kerja..................................................................................................98
3.3.1 Cara Kerja I 0 Menit...................................................................98
3.3.2 Cara Kerja I 15 Menit.................................................................99
3.3.3 Cara Kerja I 30 Menit.................................................................99
BAB IV HASIL DAN PEMBAHASAN........................................................101
4.1 Hasil Pengamatan......................................................................................101
4.1.1 Tabel Hasil Pengamatan Pertumbuhan Mikroorganisme..............101
4.2 Perhitungan ..............................................................................................102
4.3 Pembahasan ..............................................................................................102
BAB V PENUTUP.........................................................................................107
5.1 Kesimpulan...............................................................................................107
5.2 Saran..........................................................................................................107
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM VII (PENGONTROLAN MIKROORGANISME)

BAB I PENDAHULUAN.............................................................................110
1.1 Latar Belakang..........................................................................................110
1.2 Tujuan Praktikum......................................................................................111
BAB II TINJAUAN PUSTAKA...................................................................112
6
BAB III METODE KERJA...........................................................................118
3.1 Waktu dan Tempat.....................................................................................118
3.2 Alat dan Bahan..........................................................................................118
3.2.1 Alat................................................................................................118
3.2.2 Bahan.............................................................................................118
3.3 Cara Kerja.................................................................................................119
3.3.1 Cara Kerja Desinfektan Wipol......................................................119
3.3.2 Daya Kerja pada Ekstrak Langsat.................................................120
BAB IV HASIL DAN PEMBAHASAN........................................................121
4.1 Hasil Pengamatan......................................................................................121
4.1.1 Tabel Indeks Anti Mikrobial.........................................................121
4.2 Pembahasan...............................................................................................121
BAB V PENUTUP.........................................................................................127
5.1 Kesimpulan...............................................................................................127
5.2 Saran..........................................................................................................127
DAFTAR PUSTAKA
LAMPIRAN

PRAKTIKUM VIII (ANALISA KONTAMINASI MIKROORGANISME

PADA BAHAN CAIR)
BAB I PENDAHULUAN............................................................................129
1.1 Latar Belakang..........................................................................................129
1.2 Tujuan Praktikum......................................................................................130
BAB II TINJAUAN PUSTAKA...................................................................131
BAB III METODE KERJA...........................................................................136
3.1 Waktu dan Tempat.....................................................................................136
3.2 Alat dan Bahan..........................................................................................136
3.2.1 Alat................................................................................................136
3.2.2 Bahan.............................................................................................137
3.3 Cara Kerja.................................................................................................137
3.3.1 Pembuatan Media (LB, BGLBB, EMBA dan NaCl)....................137
3.3.2 Uji Pendugaan...............................................................................138
3.3.3 Uji Penegasan................................................................................139
3.3.4 Uji Pelengkap................................................................................140
BAB IV HASIL DAN PEMBAHASAN........................................................142
4.1 Hasil Pengamatan......................................................................................142
4.1.1 Tabel Hasil Pengamatan Dari Sampel Air Sirup...........................142
4.2 Perhitungan...............................................................................................142
4.3 Pembahasan ..............................................................................................142
BAB V PENUTUP.........................................................................................150
5.1 Kesimpulan...............................................................................................150
5.2 Saran..........................................................................................................150
DAFTAR PUSTAKA
LAMPIRAN