LEMBAR KERJA PESERTA DIDIK

(LKPD)

Mata Pelajaran : IPA

Nama :
Kelas :

Judul Praktikum : Morfologi Bakteri

Tujuan Praktikum : Mengetahui Bentuk Bakteri

Dasar Teori :

Bakteri dapat berkembang pada berbagai lingkungan, baik lingkungan yang ekstrem
maupun lingkungan normal di tanah dan perairan tempat sebagian besar spesies lain ditemukan.
Kemampuan bakteri beradaptasi pada berbagai habitat menegaskan mengapa bakteri terutama
prokariota adalah organisme yang paling banyak melimpah dibumi.
Bentuk bakteri yang paling umum adalah bulat, batang dan spiral. Bakteri yang berbentuk
bulat biasanya menyerupai bola. Bakteri dapat terjadi secara tunggal, dalam rantai dua sel atau
lebih, dan dalam kelompok yang menyerupai tandan buah anggur. Bakteri yang berbentuk batang
biasanya soliter, tetapi dalam beberapa bentuk batang tersusun dalam rantai. Bakteri spiral
biasanya terlihat seperti pembuka botol, bakteri spiral lainnya menyerupai koma, atau gulungan
longgar.

Alat dan Bahan :

Alat
- Mikroskop
- Kaca Benda dan Penutup Objek
- Jarum Inkubasi
- Kapas
- Alkohol
- Metilen Blue
- Tusuk Gigi
- Pipet

Bahan
- Nasi Basi
- Kentang Busuk
- Kotoran Gigi
Cara Kerja:
1. Siapkan nasi basi, kentang busuk dan kotoran yang berasal dari gigi, kemudian siapkan
juga 3 gelas objek dan penutupnya, lalu bersihkan dengan alkohol menggunakan kapas
atau tisu.
2. Sterilkan jarum inokulasi dengan memanaskan di atas pembakar spritus.
3. Ambillah kotoran gigi dengan tusuk gigi, serta kentang busuk dan nasi basi dengan
menggunakan inokulasi yang steril.
4. Oleskan secara merata di atas kaca praparat yang sebelumnya telah ditetesi air kemudian
ratakan bahan itu setipisnya, lalu keringkan di atas nyala api spritus.
5. Teteskan metilen blue atau tinta cina, keringkan selama 2 menit
6. Cucilah dengan air agar pewarna tersebut hilang.
7. Keringkan dengan cara diangin-anginkan.
8. Amatilah dibawah mikroskop, mulai dari pembesaran kecil sampai yang besar.
9. Gambarlah bentuk-bentuk bakteri yang dapat anda temukan dan bandingkan dengan charta
sel bakteri ke dalam tabel pengamatan.

Hasil Pengamatan:

Koloni Pengamatan Koloni

Gambar Keterangan
Bakteri Warna Jumlah Bentuk
Kentang
Busuk

Nasi Basi

Kotoran Gigi

Pembahasan:
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Kesimpulan:

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Tugas:

1. Ada berapa macam bentuk bakteri yang berasal dari kotoran gigi, kentang busuk, dan basi?
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2. Bagaimana susunan dari masing-masing bahan tersebut?
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3. Bandingkan hasil pengamatan anda dengan charta sel bakteri yang tersedia! Berdasarkan
bentuknya, golongan apa bakteri anda temukan?
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