DAFTAR ISI

Halaman

DAFTAR TABEL...................................................................................... iii

DAFTAR GAMBAR.................................................................................. iv

I. PENDAHULUAN................................................................................... 1
A. Latar Belakang................................................................................... 1
B. Tujuan Penelitian................................................................................ 3
C. Manfaat Penelitian.............................................................................. 3
D. Kerangka Pemikiran........................................................................... 4
E. Hipotesis............................................................................................. 6

II. TINJAUAN PUSTAKA........................................................................ 7

A. Tanaman Nanas.................................................................................. 7
B. Daun Nanas........................................................................................ 8
C. Fermentasi.......................................................................................... 9
D. Aspergillus Niger................................................................................ 11
E. Kecernaan........................................................................................... 12
F. Volatile Fatty Acids (VFA)................................................................. 14
G. Kadar Amonia (NH3) ......................................................................... 15
H. Teknik In Vitro................................................................................... 16

III. BAHAN DAN METODE PENELITIAN.......................................... 18

A. Waktu dan Tempat Penelitian.......................................................... 18
B. Bahan dan Alat Penelitian................................................................ 18
1. Bahan Penelitian.......................................................................... 18
2. Alat Penelitian.............................................................................. 19
C. Metode Penelitian............................................................................. 19
 1. Rancangan Percobaan.................................................................. 19
2. Analisis Data................................................................................ 20
D. Pelaksanaan Penelitian..................................................................... 20
1. Perbanyakan Aspergillus niger.................................................... 20
2. Fermentasi Daun Nanas............................................................... 21
3. Analisis Kecernaan In Vitro......................................................... 21
a. Pembuatan larutan Mc Doughal (Saliva Buatan)..................... 21
b. Pengambilan cairan rumen....................................................... 22
c. Pencampuran cairan rumen dan fermentasi daun nanas.......... 23
d. Konsentrasi VFA..................................................................... 23
e. Konsentrasi NH3....................................................................... 24

DAFTAR PUSTAKA................................................................................. 25
 DAFTAR TABEL

Tabel Halaman
1. Bahan penyusun larutan Mc Doughal (saliva buatan)............................. 22
 DAFTAR GAMBAR

Gambar Halaman
1. Tata letak percobaan................................................................................ 19
2. Skema Perbanyakan mikroba Aspergillus Niger..................................... 20
3. Skema pembuatan fermentasi daun nanas............................................... 21
4. Skema pembuatan larutan Mc Doughal (saliva buatan).......................... 21
5. Skema pengambilan cairan rumen........................................................... 22