DAFTAR ISI

Halaman
DAFTAR ISI ................................................................................................... i
DAFTAR TABEL ........................................................................................... iii
DAFTAR GAMBAR ...................................................................................... iv
DAFTAR SINGKATAN ................................................................................ v

BAB I PENDAHULUAN
1.1 Latar Belakang ......................................................................................... 1
1.2 Rumusan Masalah .................................................................................... 3
1.3 Hipotesis Penelitian .................................................................................. 4
1.4 Tujuan Penelitian ..................................................................................... 4
1.4.1 Tujuan Umum ......................................................................... 4
1.4.2 Tujuan Khusus ........................................................................ 4
1.5 Manfaat Penelitian .................................................................................... 5
1.5.1 Manfaat Akademis .................................................................. 5
1.5.2 Tujuan Untuk Penelitian ......................................................... 5

BAB II TINJAUAN PUSTAKA

2.1 Otitis Media Supuratif Kronis ................................................... 10
2.1.1 Definisi ........................................................................ 10
2.1.2 Klasifikasi ..................................................................... 10
2.1.3 Etiologi ........................................................................ 12
2.1.4 Patogenesis .................................................................. 13
2.2 Kolesteatoma ........................................................................... 31
2.2.1 Definisi ........................................................................ 10
2.2.2 Epidemiologi ................................................................ 10
2.2.3 Patogenesis .................................................................. 12
2.2.4 Histopatologi ............................................................... 13
2.2.5 Stadium Kolesteatoma ................................................. 13
2.2.6 Penatalaksanaan OMSK Tipe Bahaya ......................... 13
2.2.7 Komplikasi OMSK Tipe Bahaya ................................. 20
2.3 Interleukin 1α............................................................................ 20
2.4 Interleukin 1α dan Kolesteatoma ............................................ 21
2.5 Biakan Keratinosit Kolesteatoma ............................................ 23
2.6 Poguntano (Picria fel-terrae Merr.) ........................................ 24
2.6.1 Puguntano dan Inflamasi ............................................. 26
2.7 Kerangka Teori ......................................................................... 27
2.8 Kerangka Konsep ..................................................................... 28

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BAB III METODE PENELITIAN
3.1 Jenis dan Rancangan Penelitian ............................................ 29
3.2 Waktu dan Tempat Penelitian ............................................... 29
3.3 Sampel Penelitian .................................................................. 29
3.3.1 Pengukuran Ekspresi IL-1α .................................. 29
3.4 Pemilihan Sampel ................................................................. 30
3.4.1 Kriteria Inklusi ...................................................... 30
3.4.2 Kriteria Eksklusi ................................................... 30
3.5 Alat dan Bahan Penelitian ...................................................... 30
3.5.1 Alat ........................................................................ 30
3.5.2 Bahan .................................................................... 31
3.6 Definisi Operasional .............................................................. 32
3.7 Proses Perlakuan Terhadap Matriks Kolesteatoma ................ 33
3.8 Proses Pembuatan Ekstrak Ethanol Puguntano ..................... 34
3.9 Analisa Data ........................................................................... 35

BAB IV HASIL PENELITIAN

4.1 Kondisi Awal .......................................................................... 36
4.2 Setelah Perlakuan ................................................................... 36
4.3 Uji Statistik ............................................................................. 38

BAB V PEMBAHASAN ............................................................................. 39

BAB VI KESIMPULAN DAN SARAN

6.1 Kesimpulan ............................................................................. 42
6.2 Saran ....................................................................................... 42

DAFTAR PUSTAKA ...................................................................................... 43

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 DAFTAR TABEL

No Judul Tabel Halaman

Tabel 1 Prevalensi OMSK oleh WHO ................................................... 12
Tabel 4.1 Kadar IL-1α pada sampel sebelum perlakuan ............................ 36
Tabel 4.2 Kadar IL-1α pada sampel sebelum perlakuan………………... 37
Tabel 4.3 Hasil Uji ANOVA antara perlakuan terhadap kontrol ............... 38

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 DAFTAR GAMBAR

No Judul Gambar Hal

Gambar 1 Patogenesis Otitis Media ..................................................... 8

Gambar 2 Histopatologi Kolesteatoma ................................................ 16
Gambar 2.3 Daun Poguntano (Picria fel-terrae Merr) ........................... 25
Gambar 3.8.1 Simplisia Daun Puguntano dan Hasil Ekstrak Ethanol
Puguntano ........................................................................... 35
Gambar 4.1 Kadar IL-1α pada masing-masing ekstrak ethanol daun
Puguntano dibandingkan dengan kontrol ............................. 37

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 DAFTAR SINGKATAN

COX Cyclooxygenase
CRP C-reactive protein
ELISA Enzyme-Linked Immunosorbent Assay
IL-1α Interleukin-1α
iNOS Inducible nitric oxide synthase
OMSK Otitis Media Supuratif Kronis