PEMICU 3

MUTAGENESIS
Kelompok 10 :
- Engelina Melisa
- Luqyaanaa Mursidah
- Maria Gabriella
- Rizki Ananda
- Salsabila Posmaryana Utami

OUTLINE
1. Protein Stability
1.1.Free energy dan stabilitas
1.1.1. Folded Protein
1.1.2. Non-Folded Protein
1.2.Parameter
1.2.1. Disulfide Bond
1.2.2. Relative Conformation Entropy
1.2.3. Hidrogen Bonds
1.2.4. Electrostatic Interaction
1.2.5. Hydrophobicity
1.2.6. Helix Stabilization
1.3.Website pengecekan stabilitas
1.4.Cara meningkatkan stabilitas
2. Sitokrom c
2.1.Definition
2.2.Karakteristik kimia dan fisika
2.3.Peran dalam stabilisasi
3. Yeast iso-1-cytochrome c
3.1.Definition
3.2.Sequence cdna
3.3.Analisis DSC
3.4.Redox form
3.5.Mutant protein dan maksud dari kodenya masing-masing
3.5.1. T102C
3.5.2. Q69E/T102C
3.5.3. K96A/T102C
3.5.4. Q69E/Q96A/T102C
4. Mutagenesis
4.1.Definition
4.2.Method
4.2.1. Random Mutagenesis
4.2.2. Site-directed
4.2.3. Kombinationial
4.2.4. Insertional
 4.2.5. Homologus
4.3.Mekanisme dasar
4.4.Approaches
4.4.1. Kunkel's Method
4.4.2. Cassette Mutagenesis
4.4.3. PCR Site-Directed Mutagenesis
4.4.4. Whole Plasmid Mutagenesis
4.4.5. In Vivo Site-Directed Mutagenesis Methods
4.4.6. CRISPR
5. Prosedur mutagenesis yeast iso-1-cythochrome
5.1.Menentukan daerah penyisipan
5.2.Desain primer
5.3.PCR
5.4.Penyisipan
5.5.Pemisahan mutan – wildtype
5.6.Transformasi