I.

Data Pengamatan
I.1 Kurva Kalibrasi Parasetamol
a. Paracetamol
No Sampel Absorbansi
1 Menit ke 0 0.61
2 1 0.61
3 2 0.61
4 3 0.61
5 4 0.61
6 5 0.61
7 6 0.62
8 7 0.62
9 8 0.62
10 9 0.62
11 10 0.62
Persamaan regresi pada panjang gelombang 248 nm : (Tulandi,dkk,
2015)
y = 0.0218x + 0.3462
r = 0.9982

b. Hidrokortison
No Sampel Absorbansi
1 Menit ke 0 0.0072
2 1 0.0113
3 2 0.0153
4 3 0.0194
5 4 0.0235
6 5 0.0296
7 6 0.0398
8 7 0.0510
 9 8 0.0602
10 9 0.0806
11 10 0.62
Persamaan regresi dengan panjang gelombang 257 nm : (Hayun,
dkk,2014)
Y = (-9,62131.10-4) + (2,01419.10-3)x
r = 0,9999
I.2 Tabel dan Perhitungan Konsentrasi Kadar Disolusi (Paracetamol)
No Sampel Absorbansi
1 Menit ke 0 -0.023
2 5 0.023
3 10 -0.204
4 15 0.046
5 20 -0.126
6 25 -0.294
7 30 -0.047
8 35 -0.088
9 40 -0.079
10 45 0.165
11 50 0.023
12 55 0.067
13 60 0.048

y = 0.0218x + 0.3462
Menit ke-0 :
-0.023 = 0.0218x + 0.3462
0.0218x = 0.3462 + 0.023
0.0218x = 0.3692
X0 = 16.93 mg/L
Menit ke-5 :
0.023 = 0.0218x + 0.3462
 0.0218x = 0.3462 - 0.023
0.0218x = 0.3232
X5 = 14.82 mg/L
Menit ke-10 :
-0.204 = 0.0218x + 0.3462
0.0218x = 0.3462 + 0.023
0.0218x = 0.3692
X10 = 16.93 mg/L
Menit ke-15 :
0.046 = 0.0218x + 0.3462
0.0218x = 0.3462 - 0.046
0.0218x = 0.3002
X15 = 13.77 mg/L
Menit ke-20 :
-0.126 = 0.0218x + 0.3462
0.0218x = 0.3462 + 0.126
0.0218x = 0.4722
X20 = 21.66 mg/L
Menit ke-25 :
-0.294 = 0.0218x + 0.3462
0.0218x = 0.3462 + 0.294
0.0218x = 0.6402
X25 = 29.36 mg/L
Menit ke-30 :
-0.047 = 0.0218x + 0.3462
0.0218x = 0.3462 + 0.047
0.0218x = 0.3932
X30 = 18.03 mg/L
Menit ke-35 :
-0.088 = 0.0218x + 0.3462
0.0218x = 0.3462 + 0.088
 0.0218x = 0.4342
X35 = 19.91 mg/L
Menit ke-40 :
-0.079 = 0.0218x + 0.3462
0.0218x = 0.3462 + 0.079
0.0218x = 0.4252
X40 = 19.50 mg/L
Menit ke-45 :
0.165 = 0.0218x + 0.3462
0.0218x = 0.3462 - 0.165
0.0218x = 0.1812
X45 = 8.31 mg/L
Menit ke-50 :
0.023 = 0.0218x + 0.3462
0.0218x = 0.3462 - 0.023
0.0218x = 0.3232
X50 = 14.82 mg/L
Menit ke-55 :
0.067 = 0.0218x + 0.3462
0.0218x = 0.3462 - 0.067
0.0218x = 0.2792
X55 = 12.80 mg/L

Menit ke-60 :
0.048 = 0.0218x + 0.3462
0.0218x = 0.3462 - 0.048
0.0218x = 0.2982
X60 = 13.67 mg/L
= 0.22
I.3 Tabel dan Perhitungan Konsentrasi kadar Difusi (Hydrokortison)
No Sampel Absorbansi
1 Menit ke 0 -0.010
2 5 0.477
3 10 -0.314
4 15 0.000
5 20 -0.047
6 25 -0.054
7 30 -0.385
8 35 -0.028
9 40 -0.063
10 45 -0.201
11 50 0.062
12 55 0.032
13 60 -0.012

y = 0.0218x + 0.3462
Menit ke-0 :
-0.010 = (-9,62131.10-4) + (2,01419.10-3)x
- 0.010 + 9.62131.10-4 = 2,01419.10-3x
9.61131.10-4 = 2,01419.10-3x

X0 = 4.7717 x 10-1

= 0.47717 mg/L

Menit ke-5

0.477 = (-9,62131.10-4) + (2,01419.10-3)x

0.477 + 9.62131.10-4 = 2,01419.10-3x
10.09831 x 10-4 = 2.01419 x 10-3x

X5 = 0.501 mg/L

Menit ke-10
-0.314 = (-9,62131.10-4) + (2,01419.10-3)x
-0.314 + 9.62131.10-4 = 2,01419.10-3x
9.30731 x 10-4 = 2.01419 x 10-3x

X10 = 0.462 mg/L

Menit ke-15

0.000 = (-9,62131.10-4) + (2,01419.10-3)x

0.000+ 9.62131.10-4 = 2,01419.10-3x
9.62131.10-4 = 2.01419 x 10-3x

X15 = 0.477 mg/L

Menit ke-20

-0.047 = (-9,62131.10-4) + (2,01419.10-3)x

-0.047+ 9.62131.10-4 = 2,01419.10-3x
9.57431.10-4 = 2.01419 x 10-3x

X20 = 0.475 mg/L

Menit ke-25

-0.054 = (-9,62131.10-4) + (2,01419.10-3)x

-0.054 + 9.62131.10-4 = 2,01419.10-3x
9.56731.10-4 = 2.01419 x 10-3x

X25 = 0.474 mg/L

Menit ke-30

-0.385 = (-9,62131.10-4) + (2,01419.10-3)x

-0.385 + 9.62131.10-4 = 2,01419.10-3x
9.23631.10-4 = 2.01419 x 10-3x

X30 = 0.458 mg/L

Menit ke-35

-0.028 = (-9,62131.10-4) + (2,01419.10-3)x

-0.028 + 9.62131.10-4 = 2,01419.10-3x
 9.59331.10-4 = 2.01419 x 10-3x

X35 = 0.476 mg/L

Menit ke-40

-0.063 = (-9,62131.10-4) + (2,01419.10-3)x

-0.063 + 9.62131.10-4 = 2,01419.10-3x
9.55831.10-4 = 2.01419 x 10-3x

X40 = 0.474 mg/L

Menit ke-45

-0.201 = (-9,62131.10-4) + (2,01419.10-3)x

-0.201+ 9.62131.10-4 = 2,01419.10-3x
9.42031.10-4 = 2.01419 x 10-3x

X45 = 0.467 mg/L

Menit ke-50

0.062 = (-9,62131.10-4) + (2,01419.10-3)x

0.062+ 9.62131.10-4 = 2,01419.10-3x
9.68331.10-4 = 2.01419 x 10-3x

X50 = 0.480 mg/L

Menit ke-55

0.032 = (-9,62131.10-4) + (2,01419.10-3)x

0.032+ 9.62131.10-4 = 2,01419.10-3x
9.65331.10-4 = 2.01419 x 10-3x

X55 = 0.479 mg/L

Menit ke-60

-0.012 = (-9,62131.10-4) + (2,01419.10-3)x

-0.012+ 9.62131.10-4 = 2,01419.10-3x
9.60931.10-4 = 2.01419 x 10-3x

X60 = 0. 47708 mg/L

Tulandi, G.P., dkk. 2015. Validasi Metode Analisis untuk Penetapan Kadar Paracetamol
Dalam Sediaan Tablet Secara Spektrofotometri Ultraviolet. Jurnal Ilmiah
Farmasi. Vol. 4 (4).

Hayun, dkk, 2014. Penetapan Kadar Hidrokortison Asetat Dalam Sediaan Krim
Mengandung Pengawet Nipagin Secara Spektrofotometri Derivatif Orde
Pertama. Pharm Sci Res.