Hasil Uji Aktivitas Antioksidan

Abs

C Log C 1 2 3 Rata-rata %AA Probit IC50

Kontrol - 0,722 0,778 0,577 0.69233333 - -

31,25 1.49485002 0,147 0,147 0,148 0.14733333 78.719307 5,79

1.795880017 0.17033333 75.397208 5,68 229.39

62,5 0,172 0,166 0,173
ppm
125 2.096910013 0,225 0,223 0,225 0.22433333 67.597496 5,45 (sangat
lemah)
250 2.397940009 0,317 0,308 0,323 0.316 54.357246 5,11

500 2.698970004 0,497 0,488 0,509 0.498 28.069331 4,42

6
f(x) = − 1.09955819777205 x + 7.59567459434463
5 R² = 0.900254724064361

4
Nilai Probit

0
1.4 1.6 1.8 2 2.2 2.4 2.6 2.8
Log Konsentrasi

y=ax+b
Y 5
A -1.0996
B 7.5957

5= -1.0996x +
7.5957

2.36058
X= 6
IC50 = 229.395
 9

Hasil uji aktivitas sitotoksik dengan analisis probit

Jumlah mati pada Konsentrasi (ppm)

Replikasi Sampel
Control
(-) 31.25 62.5 125 250 500 1000
1 0 2 3 4 0 7 10
2 0 3 4 3 5 8 10
3 0 2 3 4 4 7 10
Total 0 7 10 11 9 22 30
penghambatan
%penghambatan 0% 23.33% 33.33% 36.66% 30% 73.33% 100%
Harga probit - 4.2699 4.5699 4.6598 4.48 5.6199 8.09
IC50 = 129.716159 < 1000 ppm (toksik)

9
8
Log C Probit 7
1.49485 4.2699 6 f(x) = 2.0947793527935 x + 0.573723846381392
R² = 0.658926518093087
Nilai Probit

1.79588 4.5699 5
4
2.09691 4.6598 3
2.39794 4.48 2
2.69897 5.6199 1
0
3 8.09 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2
Log Konsentrasi

y= ax + b
y 5
a 2.0948
b 0.5737

5= 2.0948x + 0.5737

x= 2.11299408
IC50 = 129.716159
 Hasil uji aktivitas sitotoksik dengan analisis reed & muench

C L Jum Teraku M
o lah mulasi x or
( g Rasio = al
(x+ y)
p C M H x y j it
p a i u as
m t d m %
) i u l
p a
h
3 1. 7 2 7 9 9 0.07 7
1 4 3 1 8
. 9
2 4
5 8
5
6 1. 1 2 1 6 8 0.2 2
2 7 0 0 7 8 5 0
. 9
5 5
8
8
1 2. 1 1 2 4 7 0.36 3
2 0 1 9 8 8 6 6
5 9
6
9
1
2 2. 1 2 4 2 7 0.61 6
5 3 9 1 7 9 6 1
0 9
7
9
4
5 2. 2 8 6 8 7 0.89 8
0 6 2 9 7 9
0 9
8
9
7
1 3 1 0 7 0 7 1 1
0 0 9 9 0
0 0
0
IC50 = 181.134 ppm <1000 ppm (toksik)
 50 %−a
Nilai h =
b−a

50 %−36 %
=
61 %−36 %

14 %
=
25 %

= 0.56

k
Nilai I = Log
s

250
=
125

= Log 2

= 0.30

Nilai g = h x I

= 0.56 x 0.30

= 0.168

Nilai Y = g + Log S

= 0.168 + 2.09

= 2.258

IC50 = 181.134 ppm <1000 ppm (toksik)