HASIL VERIFIKASI TPC METODE POUR PLATE PADA SAM

Hasil TPC (kol/ml) Selisih Rata-rata

Kode Sampel
Cawan 1 Log 1 Cawan 2 Log 2 log1-Log2 Xi
1 87 1.9395 99 1.9956 -0.0561 1.9676
2 103 2.0128 106 2.0253 -0.0125 2.0191
3 99 1.9956 100 2.0000 -0.0044 1.9978
4 104 2.0170 120 2.0792 -0.0621 2.0481
5 107 2.0294 78 1.8921 0.1373 1.9607
6 102 2.0086 87 1.9395 0.0691 1.9741
7 2200 3.3424 1300 3.1139 0.2285 3.2282
8 1600 3.2041 1900 3.2788 -0.0746 3.2414
9 900 2.9542 1000 3.0000 -0.0458 2.9771
10 7000 3.8451 5500 3.7404 0.1047 3.7927
11 11000 4.0414 6500 3.8129 0.2285 3.9272
12 5200 3.7160 5500 3.7404 -0.0244 3.7282
13 10800 4.0334 10100 4.0043 0.0291 4.0189
14 12000 4.0792 17400 4.2405 -0.1614 4.1599
15 11700 4.0682 14100 4.1492 -0.0810 4.1087
16 15200 4.1818 11400 4.0569 0.1249 4.1194
17 17000 4.2304 14500 4.1614 0.0691 4.1959
18 13500 4.1303 11900 4.0755 0.0548 4.1029
n
S

Personal Repeatability RSD

Technician A

Hasil TPC (kol/ml) Selisih Rata-rata

Kode Sampel
Cawan 1 Log 1 Cawan 2 Log 2 log1-Log2 Xi
1 87 1.9395 99 1.9956 -0.0561 1.9676
2 99 1.9956 100 2.0000 -0.0044 1.9978
3 107 2.0294 78 1.8921 0.1373 1.9607
4 2200 3.3424 1300 3.1139 0.2285 3.2282
5 900 2.9542 1000 3.0000 -0.0458 2.9771
6 11000 4.0414 6500 3.8129 0.2285 3.9272
7 10800 4.0334 10100 4.0043 0.0291 4.0189
8 11700 4.0682 14100 4.1492 -0.0810 4.1087
 9 17000 4.2304 14500 4.1614 0.0691 4.1959
n
S

Technician B

Hasil TPC (kol/ml) Selisih Rata-rata

Kode Sampel
Cawan 1 Log 1 Cawan 2 Log 2 log1-Log2 Xi
1 103 2.0128 106 2.0253 -0.0125 2.0191
2 104 2.0170 120 2.0792 -0.0621 2.0481
3 102 2.0086 87 1.9395 0.0691 1.9741
4 1600 3.2041 1900 3.2788 -0.0746 3.2414
5 7000 3.8451 5500 3.7404 0.1047 3.7927
6 5200 3.7160 5500 3.7404 -0.0244 3.7282
7 12000 4.0792 17400 4.2405 -0.1614 4.1599
8 15200 4.1818 11400 4.0569 0.1249 4.1194
9 13500 4.1303 11900 4.0755 0.0548 4.1029
n
S
R PLATE PADA SAMPEL AMDK

Selisih/Rata-rata Selisih/Rata-rata
Technician
(log1-Log2)/Xi [(Log1-log2)/Xi]2
-0.0285 0.000813 A
-0.0062 0.000038 B
-0.0022 0.000005 A
-0.0303 0.000921 B
0.0700 0.004903 A
0.0350 0.001225 B
0.0708 0.005009 A
-0.0230 0.000530 B
-0.0154 0.000236 A
0.0276 0.000763 B
0.0582 0.003385 A
-0.0065 0.000043 B
0.0072 0.000052 A
-0.0388 0.001505 B
-0.0197 0.000389 A
0.0303 0.000920 B
0.0165 0.000271 A
0.0134 0.000178 B
18
0.021186
RSD 0.0243
CV% 2.43

Selisih/Rata-rata Selisih/Rata-rata
Technician
(log1-Log2)/Xi [(Log1-log2)/Xi]2
-0.0285 0.000813 A
-0.0022 0.000005 A
0.0700 0.004903 A
0.0708 0.005009 A
-0.0154 0.000236 A
0.0582 0.003385 A
0.0072 0.000052 A
-0.0197 0.000389 A
 0.0165 0.000271 A
9
0.015064
RSD 0.0289
CV% 2.89

Selisih/Rata-rata Selisih/Rata-rata
Technician
(log1-Log2)/Xi [(Log1-log2)/Xi]2
-0.0062 0.000038 B
-0.0303 0.000921 B
0.0350 0.001225 B
-0.0230 0.000530 B
0.0276 0.000763 B
-0.0065 0.000043 B
-0.0388 0.001505 B
0.0303 0.000920 B
0.0134 0.000178 B
9
0.006122
RSD 0.0184
CV% 1.84
Variasi suhu dan waktu inkubasi

dibuat skema dalam 2 kondisi ekstim

Contoh : sesuai prosedur cara kerja diinformasikan bahwa kondisi optimum inkubasi pada suhu 35 oC ± 2 oC, selama 24 jam ± 2

Parameter Kondisi minumum Kondisi maksimum

Suhu 34 oC 36 oC
Waktu 22 jam 26 jam

Desain percobannya :
Waktu Inkubasi
Kondisi 1 suhu 35 oC waktu 22 jam
Kondisi 2 suhu 35 oC waktu 24 jam
Kondisi 3 suhu 35 oC waktu 26 jam

Waktu Inkubasi
Kosentrasi analit Kondisi 1 Kondisi 2 Kondisi 3
Rendah 30 34 38
35 38 40
basi pada suhu 35 oC ± 2 oC, selama 24 jam ± 2 jam.

Suhu Inkubasi
Kondisi 1 suhu 33 oC waktu 24 jam
Kondisi 2 suhu 35 oC waktu 24 jam
Kondisi 3 suhu 37 oC waktu 24 jam

Suhu Inkubasi
Kosentrasi analit Kondisi 1 Kondisi 2 Kondisi 3
Sedang 116 125 135
126 135 147
Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Column 1 2 65 32.5 12.5
Column 2 2 72 36 8
Column 3 2 78 39 2

ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 42.33333 2 21.16667 2.822222 0.204445 9.552094
Within Groups 22.5 3 7.5

Total 64.83333 5
 Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Column 1 2 242 121 50
Column 2 2 260 130 50
Column 3 2 282 141 72

ANOVA
Source of VariationSS df MS F P-value F crit
Between G 401.3333 2 200.6667 3.5 0.164317 9.552094
Within Gro 172 3 57.33333

Total 573.3333 5