LAPORAN PRAKTIK MAGANG

IDENTIFIKASI BAKTERI DAN JUMLAH TOTAL

KONTAMINAN PADA IKAN BANDENG
DAN IKAN KEMBUNG ( DI BP2MHKP BANDUNG

F a rh a n M a u la R a m a d h a n
2 2 . 4 .0 4 .0 2 9

TEKNIK PENGOLAHAN PRODUK PERIKANAN

POLITEKNIK KELAUTAN DAN PERIKANAN KARAWANG
KEMENTERIAN KELAUTAN DAN PERIKANAN
2023
 LAPORAN PRAKTIK MAGANG

IDENTIFIKASI BAKTERI DAN JUMLAH TOTAL

KONTAMINAN PADA IKAN BANDENG
DAN IKAN KEMBUNG ( DI BP2MHKP BANDUNG

F a rh a n M a u la R a m a d h a n
2 2 . 4 .0 4 .0 2 9

S eb a g a i s ala h s a tu s ya ra t u n tu k m e la n ju tk a n k e s e m e s ter b erik u tn ya

TEKNIK PENGOLAHAN PRODUK PERIKANAN

POLITEKNIK KELAUTAN DAN PERIKANAN KARAWANG
KEMENTERIAN KELAUTAN DAN PERIKANAN
2023
 LEMBAR PERSETUJUAN

Judul : Id en tifik a s i B a k teri D a n J u m la h

T o ta l K o n ta m in a n P a d a Ik a n B a n d en g
Dan Ik a n K em b u n g
( D i B a la i K a ra n tin a Ik a n ,
P en g en d a lia n M u tu D a n K e am a n a n H a s il
P erik a n an B a n d u n g

N a m a T a ru n a : F a rh a n M a u la R a m a d h a n

N IT : 2 2 .4 .0 4 .0 2 9

D is etu ju i o leh :

P em b im b in g I P e m b im b in g II

d rh . T s a n i Is m i Is d a riya h d rh . S a rtik a R a h m a E
NIP.1976063020101220022 N IP

iii
 LEMBAR PENGESAHAN

Judul : Id e n tifik a s i B a k teri D a n J u m la h

T o ta l K o n ta m in an P ad a Ik a n B a n d en g
D a n Ik an K e m b u n g (
D i B a la i K a ra n tin a Ik a n , P en g en d alia n M u tu
D a n K e a m a n a n H a s il P erik a n a n B a n d u n g

N a m a T a ru n a : F a rh a n M a u la R a m a d h a n
N IT : 2 2 .4 .0 4 .0 2 9

D is etu ju i o leh :

S ek reta ris P ro g ra m S tu d i K etu a Pro g ra m S tu d i

T ek n ik P en g o la h a n Pro d u k P erik a n a n T e k n ik P en g o la h a n P ro d u k P erik a n an

R iz a R izk ia h .,S ,S i. M .I.L R o m a u li J u lia n a N a p itu p u lu ,S . S t.P i,. M . Sc

NIP. 199203232023212046 N IP . 1 9 8 4 0 7 1 9 2 0 0 7 0 1 2 0 0 2

iv
 KATA PENGANTAR

S eg a la p uji d a n s yu k u r p e n u lis p a n ja tk a n k eh a d ira t A lla h S W T k a re n a

b e rk a t ra h m a t, k a ru n ia d a n h id a ya h -N ya p e n u lis d a p a t m en ye le s a ik a n
la p o ra n m a g an g ya n g b erju d u l “Id en tif ik a s i B ak te ri D an
J u m la h T o tal K o n ta m in a n Pa d a Ik a n B a n d e ng
D a n Ik a n K em b u n g ( D i B a la i P e n g e n d a lia n d an P e ng a w a s a n
M u tu D a n H a s il K ela u ta n P e rik a n a n B a nd u n g . ”
La p o ra n in i d is u s u n b erd a s a rk a n p en g a la m a n p e n u lis s ela m a
m a g a n g d i La b o ra to riu m M ik ro b io lo g i M u tu B a la i K ara n tina , P e n g e n d a lia n
M u tu D a n K ea m a n a n H a s il P e rik a n a n B a n d u n g . ya n g b erlo k a s i d i J l.
C ia w ita li N o . 4 4 , K elu ra h a n C iteu reu p , K e c a m a ta n C im a h i U ta ra , K o ta
C im a hi. T erh itu n g m u la i d a ri ta n g g a l 0 9 O k to b e r 2 0 2 3 s / d 0 9 D e s em b e r
20 23 .
P en u lis m en ya d a ri s ep en u h n ya , te la h b a ny ak m en d a p a tk a n
d u k u n g a n, b im b in g a n d a n m o tiva s i d a ri b erb a g a i p ih a k ya n g te la h
m e n yu m b a n g k a n p ik ira n , w a k tu , te n a g a d a n s e b a g a in ya . O leh k a re n a itu ,
p a d a k es e m p a ta n in i d e n g a n s e tulu s h a ti p e n u lis m en g u c a p k a n
te rim a k a s ih k e p a d a :
1 . O ra n g tu a k u ya n g tela h m em b e rik a n d o ’a s erta d u k u n g a n , b a ik s ec a ra
m o ra l m a u p u n m a teri.
2 . B a p a k A n a k A g u n g G ed e E k a S u s ila , S .P i, M .S c , S ela k u K e p a la B a la i
K a ra n tin a , P e n g e nd a lia n M u tu D a n K ea m a n a n H a s il P erik an a n B a n d u n g .
3 . B a p a k Z a e n a l A rifin , A .M d , s ela k u P ela k s a n a K o o rd in a s i U ru s a n U m u m .
B a la i K a ra n tin a , P en g en d a lia n M u tu D a n K ea m a n a n H a s il P erik a n a n
B and ung.
4 . B a p a k Is ep W a h yu d in , S .P i, s e la k u P ela k s a n a K o o rd in a s i U ru s a n T a ta
P e lay an a n d a n O p era s io n a l B a la i K a ra n tin a , P en g en d a lia n M u tu D a n
K e a m a n a n H a s il P erik a n a n B a n d u n g .
5 . B a p a k H a ri H a rya n to , S .S t.P i, M .S i, s ela k u P ela k s a n a K o o rd in a s i U ru s a n
P e ngaw asan P en g en d a lia n dan In fo rm a s i B a la i K a ra n tin a ,
P e n g e n d a lian M u tu D a n K e a m a n a n H a s il P erik a n a n B a n d u n g
6 . Ib u d rh . T s a n i Is m i Is d ariya h , s ela k u D e p u ti M a n ag er T e k n is
L a b o ra to riu m s ek a lig u s Pe m b im b in g I d i L a b o ra to riu m M ik ro b io lo g i
B a la i K a ra n tin a , P en g en d a lia n M u tu D a n K ea m a n a n H a s il P erik a n a n
Band ung
7 . Ib u d rh S a rtik a s e la k u p em b im b in g II d i La b o ra to riu m M ik ro b io lo g i
B a la i K a ra n tin a , P en g en d a lia n M u tu D a n K ea m a n a n H a s il P erik a n a n
Band ung
8 . S e rta k e lu a rg a b es a r s a ya ya n g tela h terlib a t d a la m p en ye le s a ia n
p rak tik m a g a n g ya itu o m s u s w o n o , o m d irw a n , ta n te e lly , d a n ta n te yu n i,
ya n g tela h m em b erik a n m o tiv a s i d a n m e m b a n tu p e n ulis s ela m a
k e g ia ta n m a g a n g b erla n g s u n g

v
 DAFTAR ISI

LE M B A R P E R S E T U J U A N .................................................................................... iii
LE M B A R P E N G E S A H A N ..................................................................................... iv
K A T A P E N G A N T A R .............................................................................................. v
D A FT A R IS I ........................................................................................................... v i
D A FT A R T A B E L ................................................................................................... ix
D A FT A R G A M B A R ................................................................................................ x
D A FT A R LA M P IR A N ............................................................................................ x i
1. P E N D A H U LU A N ........................................................................................... 1
1 .1 La ta r B e la k a n g ...................................................................................... 1
1 .2 T u ju a n ......................................................................................................... 1
1 .3 M a n fa a t ...................................................................................................... 1
1 .4 W a k tu d a n L o k a s i M a g a n g ...................................................................... 1
1 .4 .1 . W a k tu ..................................................................................................... 1
1 .4 .1 . Lo k as i ..................................................................................................... 1
2 . R U A N G L IN G K U P T E M P A T M A G A N G ........................................................... 2
2 .1 S eja ra h B P2 M H K P B a n d u n g .................................................................... 2
2 .2 V is i m is i ...................................................................................................... 2
2 .3 D a s a r H u k u m B P 2 M H K P B a nd u n g ......................................................... 2
2 .4 S tru k tu r O rg a n is a s i B P 2 M H K P B a n d u n g ............................................... 3
2 .5 T u g a s P o k o k d a n F u n g s i .......................................................................... 3
3 . L A N D A S A N T E O R I ........................................................................................... 5
3 .1 D e s k rip s i Ik a n B a n d e n g ( .............................................. 5
3 .1 .1 K la s ifik a s i Ik a n B a n d e n g ( ..................................... 5
3 .1 .2 C iri – c iri ik a n B a nd en g ..................................................................... 6
3 .2 D e s k rip s i Ik a n K em b u n g ............................................... 6
3 .2 .1 K la s ifik a s i Ik a n K em b u n g ...................................... 6
3 .2 .2 C iri-c iri Ik a n K e m b u n g ........................................... 7
3 .3 ............................................................................................ 7

3 .4 C o lifo rm ...................................................................................................... 8
4 . M E T O D E P R A K T IK ........................................................................................... 9
4 .1 W a k tu d a n T e m p a t ............................................................................... 9
4 .2 M e to d e P en g a m b ila n D a ta .................................................................. 9

vi
 4 .2 .1 D a ta Prim e r ..................................................................................... 9
4 .2 .2 D a ta Se k u n d e r ................................................................................ 9
4 .2 S te rilis a s i R u a ng a n , A la t d a n M e d ia ....................................................... 9
4 .2 .1 S trelis a s i R ua n g a n ............................................................................. 9
4 .2 .3 S tre lis is a s i A la t ............................................................................ 1 0
4 .2 .4 S tre lis is a s i M ed ia ........................................................................ 1 0
4 .3 A la t, B ah a n d a n P e re a k s i ................................................................... 1 0
4 .4 M e to d e Pe m b u a ta n M ed ia U ji ............................................................... 1 2
5 . H A S IL D A N P E M B A H A S A N .......................................................................... 1 3
5 .1 . U ji O rg an o lep tik ...................................................................................... 1 3
5 .2 U ji .................................................................................................... 1 3
5 .3 d s t... . ......................................................................................................... 1 3
6 . K E SIM P U LA N D A N S A R A N .......................................................................... 1 4
6 .1 K es im p u la n .............................................................................................. 1 4
6 .2 S a ra n ........................................................................................................ 1 4
D A FT A R P U S T A K A ............................................................................................ 1 5
LA M P IR A N .......................................................................................................... 1 6
D A FT A R P U S T A K A ............................................................................................ 1 7
D A FT A R LA M P IR A N .......................................................................................... 1 8

v ii
DAFTAR TABEL

v i ii
 DAFTAR GAMBAR

G a m b a r 1 . K a n to r B P 2 M H K P B a n d u n g ............................................................ 3

ix
DAFTAR LAMPIRAN

x
 1. PENDAHULUAN

1.1 Latar Belakang

Ik a n m e ru p a k a n b a h a n m a k a n a n ya n g s e n s itif terh a d a p k e ru s a k a n .
P en a n g a n a n ya n g b a ik s a n g a t d ip e rlu k a n d a la m p ro d u k s i ik a n , en z im a ta u
m ik ro o rg a n is m e a d a la h s a la h s a tu f a k to r ya n g d a p a t m e ru s ak s tru k tu r ik a n
m u la i d a ri w a rn a , s tru k tu r, tub u h h in g g a terc iu m a ro m a tid a k s ed a p . P a d a
u m u m n ya p ro s es p em b u s u k a n ik a n te ru ta m a d is eb a b k a n o leh a k tivita s
en z im d a la m tu b u h ik a n teru ta m a d is eb ab k a n o le h a k tiv ita s e n zim d a la m
tu b u h ik a n itu s e n d iri a ta u k a ren a f a k to r ek s tern a l la in n ya . Ik a n b a n d en g
m e ru p a k a n s a la h s a tu ik a n b u d id a ya ya n g b a n ya k d ik o n s u m s i d i In d o n e s ia .
B a n ya k m a k a n a n k h as In d o n e s ia ya n g m e ng g u n a k a n ik a n b a n d e n g s eb a g a i
b a h a n d a s a rn ya . S ep erti b a n d en g p res to , b a n d en g ta n p a d u ri, o ta k o ta k
b a n d en g , d a n la in n ya . P a d a ta h u n 2 0 1 4 , p ro d u k s i ik a n b a nd en g d i In d o n es ia
m e n c a p a i 6 3 1 .1 2 5 to n a ta u 1 4 ,7 9 % d a ri to ta l s elu ruh ik a n b u d id a ya ya n g d i
p ro d u k s i.
Ik a n b a n d e n g s a n g a t m u d a h d ite m u k an d i p a s a r-p a s a r in d o n es ia ,
b a ik p a s a r tra d is io n a l m a u p u n p a s a r m o d ern . B a n ya k p ed a g a n g d i p a s a r
tra d is io na l m a u p u n p a s a r m o d e rn. B an ya k p ed a g a n g d i p a s a r ya n g tid a k
m e n g e rti p en tu in g n ya s a n ita s i u n tu k m e n ja g a k ea m a n a n b a ha n p a n g a n
ya n g d iju a l. Ik a n ik a n ya n g d iju a l d i p a s a r h a n ya d ile tak k a n p a d a la p a k
te rb u k a d a n d id ia m k a n d a la m w a k tu b e rjam -ja m . B eb era p a p e n ju a l
m e leta k k a n ik a n d i a ta s e s b a tu ya n g b elu m te rjam in k eb ers ih a n n ya .
B a n ya k n ya s a m p a h ya n g m e n u m p u k ju g a m em b u a t k o n d is i p as a r m en ja d i
tid a k h ig ie n is s e h in g g a m en d u k un g m ik ro o rg an is m e u n tuk d a p a t
b e rk e m b a n g b ia k p a d a ik a n k a re n a ik a n m e n g a n d u n g n u tris i ya n g
b e rlim p a h .
Ik a n k e m b u n g m e ru p a k a n ik a n p ela g is k ec il ya n g
s erin g k ita ju m p a i d i p era ira n p a n ta i in d o n e s ia . Ik a n k em b u ng term a s u k
ik a n y an g m e m ilik i k a n d u n g a n g iz i ya n g c u k up tin g g i. S e hin g g a tin g k a t
k o n s u m s i ik a n m e n in g k a t k a ren a ik a n m eru p a k a n s u m b er p ro tein h ew a n i
ya n g tin g g i. Ik a n k em b u n g (R a s tre llig er s p .) a d a la h s a la h s a tu je nis ik a n la u t
ya n g p em a s a ra n n ya ters eb a r lu a s d a n d ig em a ri o leh s e b a g ia n b e s a r
m a s ya ra k a t In d o n es ia (B a la d in , 2 0 0 7 ). P en a n g a n a n ik a n ya n g b a ik h a ru s
d ilen g k a p i d en g a n s a ra n a d a n fa s ilita s ya ng m en u n jan g s e m u a a k tifita s
d a la m b id a n g p e rik a n a n . M e n u ru t la p o ra n D K P S u m b a T im u r (2 0 1 8 ) ju m la h
h a s il ta n g k a p a n ik a n k em b u n g 2 ,4 7 % d a ri to ta l p ro d u k s i
ik a n p a d a ta h u n 2 0 1 8 ya k n i s eb a n ya k 1 1 .9 6 7 to n . P o ten s i ta n g k a p a n ik a n
k em b u n g y an g b e s a r in i h a ru s d i d u k u n g o le h f a s ilita s d a n c a ra p en a n g a n a n
ya n g b a ik d a n b e n a r.
B a k te ri c o lifo rm te rm a s u k fa m ili E nte ro b a c te ric ea e b e rs ifa t g ra m
n eg a tif d a n b erb en tu k b a ta n g . C o lifo rm m eru p a k a n m ik ro o rg a n is m e
in te s tin a l ya n g te rk a n d u ng d a la m ju m la h b a n ya k d a la m k o to ra n m a n us ia ,
ik a n, m a m m a lia d a n u n g g a s s eh in g g a b a k teri in i s erin g d ig u n a k a n s e b a g a i
in d ik a to r a d a n ya k o nta m ina s i k o to ra n d a n k o n d is i s a n ita s i ya n g tid a k b a ik
te rh a d a p a ir, m a k a n a n , s u s u d a n p ro d u k -p ro d uk s u s u (J a y, 1 9 9 2 ).
K o n ta m in a s i o le h b a k teri c o lif o rm d i d a la m b a h a n m a k a n a n d a la m ju m la h
tin g g i, teta p i tid a k s e la lu m en im b u lk a n p eru b a h a n d a la m h a l b a u , w a rn a

1
m a u p u n ra s a . S e m a k in tin g g i ju m la h s a lm o n ella d i d a la m s u a tu m a k a n a n ,
s em a k in be sar tim b u ln ya g eja la in f ek s i p ada o ra n g ya n g
m e n ela n / m e n g k o n s u m s i m a k a n a n ters eb u t. T o k s in ya n g d ih a s ilk a n b a k teri
s a lm o n ella d a n c o lifo rm (k h u s u s n ya E . C o li) m em p u n ya i ef ek terh a d a p
s a lura n p e n c e rn a a n d a n d ap a t m en ye b a b k a n d ia re .
E s c h eric h ia c o li d a p a t m en ye b a b k a n d ia re p a d a m a n u s ia d is eb u t
E n tero P a to g en ik E s c h eric h ia c o li (E E G ) . In fe k s i d a ri E E G d a p a t
m e n yeb a b k a n p en ya k it s ep erti k o lera d a n d is e n tri p a d a a n a k -a n a k d a n
o ra n g d e w a s a ( N u ra en i d k k , 2 0 0 0 ).
k h ir-a k h ir in i k as u s ya n g d is e b a b k a n o leh b a k teri E s c h e ric h ia c o li s e rin g
m e n ja d i p em b ic a ra a n ya n g k e ra p terja d i. B erb a g a i n eg a ra d i b e la h a n d u n ia
s a a t in i s u d a h m u la i m em p erh a tik a n a k ib a t ya n g d is eb a b k a n o leh b a k te ri in i
te rm a s u k b a h a n p a n g a n ya n g b era s a l d a ri p ro d u k p erik a n a n b a ik s eg a r
m a u p u n o la h a n . P e n yeb a b terja d in ya k a s u s ya n g d ia k ib a tk an o leh b a k teri
E s c h eric h ia c o li a d a la h k a re n a k u ra n g n ya p e n g eta h u a n d a n p en a n g a n a n
ya n g tep a t terh a d a p b a h a n p a n g a n .

1.2 Tujuan
1 . M e n g e ta h u i d a n m e ng ik u ti c a ra p en g u jia n b a k teri pad a
ik a n b a n d en g
2 . M e n g e ta h u i d an m e n g ik u ti c a ra p e n g u jia n b a k te ri p a d a ik a n
bandeng
3 . M e n g e ta h u i d a n m e n g ik u ti c a ra p en g ujian b a k teri pad a
ik a n k em b un g
4 . M e n g e ta h u i d a n m w n g ik u ti c a ra p en g u jia n b a k te ri p a d a ik a n
k em b u n g

1.3 Manfaat
1 . M e n a m b a h k e te ra m p ila n b a g a im a n a c a ra p en g u jia n b a k teri
p a d a ik a n b a n d en g
2 . M e n a m b a h k ete ra m p ila n b a g a im a n a c a ra p e n g u jia n b a k te ri
p a d a ik a n b a n d e n g
3 . M e n a m b a h k e te ra m p ila n b a g a im a n a c a ra p en g u jia n b a k teri
p a d a ik a n k em b u n g
4 . M e n a m b a h k ete ra m p ila n b a g a im a n a c a ra p e n g u jia n b a k te ri
p a d a ik a n k em b u n g

1.4 Waktu dan Lokasi Magang

1.4.1. Waktu
P ra k tik m a g a n g d ila k s a n a k a n d i La b o ra to riu m B a la i K a ra n tin a ,
P en g en d a lia n M u tu D a n K ea m a n a n H a s il Pe rik a n a n B a n d u n g , d ila k s a n a k a n
s ela m a 2 b u la n terh itu n g m u la i d a ri ta n g g a l 0 9 O k to b er 2 0 2 3 s a m p a i 0 9
D e s em b e r 2 0 2 3 .
1.4.1. Lokasi
P ra k tik K erja La p a ng d ila k s a n a k a n d i K a n to r S ta s iu n K a ra n tin a Ik a n
P en g en d a lia n M u tu d a n K e a m a n a n H a s il P e rik a n a n (S K IP M ) B a n d u n g ya n g

2
b e rlo k a s i d i J a la n C iaw ita li N o .4 4 , C ite u re u p , K e c .C im a h i U ta ra , K o ta C im a h i,
J aw a B a ra t.

3
 2. RUANG LINGKUP TEMPAT MAGANG

2.1 Sejarah BP2MHKP Bandung

P em b e n tu k a n B a la i P en g en d a lia n D a n P e n g a w a s a n M utu H a s il
K ela u tan P erik a n a n (B P P M H K P ). M e ru p a k a n s im p lifik a s i d a ri p ela k s a n a a n
im p le m en ta s i p era tu ra n p e ru n d a n g a n , tu g a s p o k o k d a n fu n g s i, v is i d a n m is i,
b iro k ra s i d a n o rien ta s i p e la ya n a n d a ri d u a in s titu s i ya k n i K a ra n tin a Ik a n d a n
La b o ra to riu m P em b in a a n d a n P en g u jia n M u tu H a s il P erik a n a n . D ila ta r
b e la k a n g i m a s ih te rd a p a tn ya p e rm a s a la h a n d a la m k e g ia ta n e k s p o r h a s il
p e rik a n a n ya n g m en ya n g k u t a s p e k p ers ya ra ta n n eg a ra tu ju a n ek s p o r d a la m
h a l m u tu , lem a h n ya p e n g a w a s a n d a n p en g en d a lia n m u tu p ro d u k p erik a n a n
tu ju a n e k s p o r ya n g b erd a m p a k m as ih terd a p a tn ya p e n o la k a n p ro d u k
p e rik a n a n a s a l In d o n e s ia o leh n eg a ra tu ju a n , d ip erlu k a n la n g k a h d a n
s tra teg i u n tu k m e n c ip ta k a n s in e rg ita s d u a in s titu s i ya n g m e m p u n ya i tu g a s
p o k o k d a n f un g s i ya n g m a s in g -m a s in g b ero rien ta s i k ep a d a k ea m a n a n
p a n g a n , p erlin d u n g a n s u m b erd a ya , p e la ya n a n k e p a d a m a s ya ra k a t d a n
m e ru p a k a n b a g ia n d a ri s is tem p erd a g a n g a n , m en ja d i s a tu o rg a n is a s i
s eb a g a i b en tu k ya n g d ia n g g a p id e a l g u n a m e n g em b a n m is i d a n tu g a s ya n g
s em a k in b erk em b an g .

G a m b a r 1 . K an t o r B P P M H K P B a n d u n g

2.2 Visi misi

● V is i
Te rw u ju d n ya T a ta K e lo la P em erin ta h a n ya n g B a ik m ela lu i Pe la ya n a n
S e rtifik a s i K es e h a ta n Ik a n d a n M u tu H a s il P erik a n a n , L em b a g a In s p ek s i,
d a n L a b o ra to riu m ya n g T e rs ta n d a rd is a s i, B erm u tu , A m a n d a n T e rp erc a ya .

● M is i
1 . M em b e rik a n p e nja m in a n k e s eh a ta n ik a n , m u tu d a n k ea m a n a n h a s il
p erik a n a n , s erta k ea m a n a n h a ya ti ik a n s e b a g a i u p a ya tin d a k a n
p erlin d u n g a n terh a d a p k e s eh a ta n m a n u s ia , ik a n d a n lin g k u n g a n .
2 . M em b e rik a n d u k u n g a n p erk em b a n g in d u s tri p erik a n a n h u lu -h ilir
m e la lu i s ta n d a rd is a s i, la ya n a n s ertif ik a s i k e s eh a ta n ik a n d a n m u tu
p erik a n a n d a la m ra n g k a p en in g k a ta n d a ya s a in g d an m e n ing k a tn ya
k in e rja e k s p o r p ro d u k p e rik a n a n .
3 . M e n in g k a tn ya p en g a w a s a n s u m b er d a ya k ela u ta n d a n p erik a n a n ya ng
in teg ratif.

4
 4 . M e n in g k a tk a n K em a m p u a n P e lay an a n P u b lik , Le m b a g a In s p e k s i d a n
La b o rato riu m P en g u ji d i B id a n g K a ra n tin a Ik a n d an P e n g e n d a lia n
M u tu K ea m a n a n H a s il P e rik a n a n d ala m u p a ya p en e ra p a n S is te m
M a n a je m e n M u tu In teg ra s i (S M M I) s e c a ra K o n s is te n , s erta.
5 . M e nin g k a tk a n T a ta K e lo la P e m e rin ta h a n ya n g B a ik

2.3 Dasar Hukum BPPMHKP Bandung

1 . U n d a n g -U n d a n g N o m o r 2 1 ta h u n 2 0 1 9 te n ta n g K a ra ntin a H ew a n , Ik a n
d an Tum buhan.
2 . P era tu ra n P em erin ta h N o m o r 1 5 ta h u n 2 0 0 2 te n ta n g K a ra n tin a Ik a n .
3 . P era tu ra n M e n te ri K P N o m o r 5 1 ta h u n 2 0 1 8 te n ta n g P e rs ya ra ta n d a n
T a ta C a ra P en erb ita n S e rtifik a t P en e ra p a n P ro g ra m M a n a je m e n M u tu
T e rp a d u / H a z a rd A n a lys is a n d C ritic a l C o n tro l Po in t.
4 . K ep u tu s a n M en teri K P N o m o r 1 7 T a h u n 2 0 2 1 ten ta n g P en e ta p a n
J e n is -J en is Pe n ya k it Ik a n K a ra n tin a , G o lo n g a n , d a n M ed ia P e m b a w a.
5 . K ep u tu s a n K ep a la B a d a n K a ra n tin a Ik a n , Pe n g e n d a lia n M u tu , d a n
K e a m a n a n H a s il P erik a n a n N o m o r 9 5 ta h u n 2 0 2 0 ten ta n g P e tu n ju k
T e k n is S ertifik as i M u tu d a n K ea m a n a n H a s il P erik an a n

ANAK AGUNG GEDE EKA SUSILA, S.Pi., M.Sc

K e p ala B P 2M HK P B an du ng

ZAENAL ARIFIN, A.Md

P ela ksan a K oordin asi Uru sa n
Um u m

HARI HARYANTO, S.St. Pi.,M .Si ISEP WAHYUDIN, S.Pi

P ela k s a na K o or d in as i U ru s a n P ela k s an a K oo r d in as i U ru s an T a ta
Pe ng a w as a n P eng e nd a lia n d an Pe la ya na n d an O pe ra s io na l
In fo r m as i

2.4 Struktur Organisasi BPPMHKP Bandung

2.5 Tugas Pokok dan Fungsi
1 . T u g a s K ep a la B P P M H K P
K ep a la Bad an m em ilik i tu g a s u n tu k m en ye len g g a ra k a n
p e rk a ra n tin a a n ik a n, p en g en d a lia n m u tu , d a n k ea m a n an h a s il p e rik a n a n ,
s erta k e a m a n a n h a ya ti ik a n .

2. Tu g a s K e p a la P ela k s a n a U ru s an U m u m
a . P ela k s a n a a n u ru s a n k eu a n g a n d a n p ela p o ra n ;
b . P e la k s a n a a n u rus a n p ers u ra ta n , k e ars ip a n , d a n k e p e g a w a ia n ; d a n
c . P e la k s a n a a n u ru s an p erlen g k a p a n d a n ru m a h ta n g g a .

3. Tu g a s Pe la k s a n a K o o rd in a s i U ru s a n P e ng a w a s a n P e n g e n d a lia n d a n
In fo rm a s i

5
 a . P ela k s a n a a n p e m a nta u a n terh a d a p H a m a d a n P e n ya k it Ik a n
K a ra n tin a , m u tu d a n k ea m a n a n h a s il p erik a n a n , d a n k ea m a n a n h a ya ti
ik a n.
b . P ela k s a n a a n p e n g a w a s a n te rh a d a p H a m a d a n P en ya k it Ik a n
K a ra n tin a d a n k e a m a na n h a ya ti ik a n .
c . P ela k s a n a a n s u rve ila n s te rh ad a p H a m a d a n P en ya k it Ik a n K a ra n tin a
d a n k ea m a n a n h a ya ti ik a n .
d . Pe la k s a n a an in s p ek s i, ve rif ik a s i, s u rv eila n s , a u d it, d a n p e n g a m b ila n
c o n to h ik a n d a n h a s il p e rik a n a n d i U n it P en g o la h a n Ik a n d a la m ra n g k a
s ertif ik a s i p e n era p a n p ro g ra m m a n ajem en m u tu terp a d u .
e . P en e ra p a n s is te m m a n a je m e n m u tu p e lay an a n o p e ra s io na l d a n
la b o ra to riu m .
f . P e n in d a k a n p e la n g g a ra n p erk a ra n tin a a n ik a n , p en g en d a lia n m u tu d a n
k ea m a n a n h a s il p e rik a n a n , d a n k e a m a n a n h a ya ti ik a n .
g . P en g u m p u la n , p en g o la h a n d a ta d an in fo rm a s i p erk a ra ntin a a n ik a n ,
p e n g en d a lia n m u tu d a n k ea m a n a n h a s il p e rik a n a n , d a n k ea m a n a n
h a ya ti ik a n .

4 . T u g a s P ela k s a n a K o o rd in a s i U ru s a n T a ta P ela ya n a n d a n O p e ras io n a l

a . P ela k s a n a a n p en c eg a h a n m a s u k d a n te rs eb a rn ya H a m a d a n P en ya k it
Ik a n K a ra n tin a d a ri lu a r ne g e ri d a n d a ri s u a tu a re a k e are a la in d i
d a la m n eg eri, a ta u k e lu a rn ya d ari d a la m w ilay ah N e g a ra R ep u b lik
In d o n es ia .
b . P e la k s a n a a n p en c e g a h a n k e lu a r d a n ters eb a rn ya H a m a d a n P en ya k it
Ik a n terten tu d a ri w ila ya h N eg a ra R ep u b lik In d o n es ia ya n g
d ip e rs ya ra tk a n N e g a ra tu ju a n.
c . P ela k s a n a a n tin d a k an k a ra n tin a terh a d a p m e d ia p e m b a w a H a m a
d a n P en ya k it Ik a n K a ra n tin a /H a m a d a n P e n ya k it Ik a n te rten tu , je n is
ik a n d ilin d u n g i, d ilara n g , d ib a ta s i, d a n in va s if, s erta b en d a la in .
d . P e la k s an a a n p e n g u jia n terh a d ap H a m a d a n P en ya k it Ik a n K a ra n tin a ,
H a m a d a n P en ya k it Ik a n te rte n tu , m u tu d a n k e am a n a n h a s il p e rik a n a n ,
d a n k ea m a n a n h a ya ti ik a n .

6
 3. LANDASAN TEORI

3.1 Deskripsi Ikan Bandeng (

Ik a n b a n d e ng ( a d a la h ik a n p a n g a n p o p u ler d i A s ia
T en g g a ra d a n O s e a n ia . Ik a n in i m e ru p a k a n s a tu -s a tu n ya s p es ies ya n g
m a s ih a d a d a la m s u k u C h a n id a e (b ers a m a en a m g en u s ta m b a h a n ya n g
d ila p o rk a n p ern a h a d a n a m u n s u d a h p u n a h) . In d o n es ia m em ilik i k e k a ya a n
h a s il p e rik a n a n ya n g m e lim p a h . S a la h s a tu je n is ik a n y an g m e n ja d i an d a la n
a d a la h ik a n b a n d e n g Ik a n b a n d e n g s a n g a t p o p u le r d i
k a lan g a n m a s ya rak a t In d o n es ia k a ren a k a n d u n g an g iz i ya n g tin g g i d a n
ra s a n ya ya n g le za t. S ela in itu , ik an b a n d en g ju g a m u d a h d item u k a n d i p a s a r
tra d is io na l. Ik a n b a n d e ng m en g a n d u n g p ro te in s ek itar 2 0 % b e ra t b a s a h d a n
lem a k 4 ,8 % b a h a n s e g a r, n a m u n ik a n in i te rm a s u k k o m o d ita s ya n g m u d a h
b u s u k . O le h k a re n a itu , k o n s u m en s eb a ik n ya m em ilih ik a n y an g m a s ih s eg a r
u n tu k d ik o n s u m s i. (W . Pa m ijia ti, 2 0 0 9 ).

3 .1 .1 K la s ifik a s i Ik a n B a n d e n g (
Ik a n b a n d e n g a d a la h ik a n p a n g a n p o p u le r d i A s ia
T en g g a ra d a n O s e a n ia . Ik a n in i m e ru p a k a n s a tu -s a tu n ya s p es ies ya n g
m a s ih a d a d a la m s u k u C h a n id a e (b ers a m a en a m g en u s ta m b a h a n ya n g
d ila p o rk a n p e rn a h a d a n a m u n s u d ah p u n a h ). D a la m b a h a s a B u g is d a n
M a k a s s a r d ik en a l s eb a g a i ik a n b o lu , d a la m B a h a s a F ilip in a s eb a g a i b a n g us ,
d a la m B a h a s a M a la ys ia s e b a g a i ik a n s u s u , d a n d a la m b a h a s a In g g ris
s eb a g a i .

Ta b e l 1 . K la s ifik a s i ik a n b a nd en g (Ch a n o s -c ha n o s )

K ingd om : A nim alia

Filum : C h o rda ta
K elas : A c tinop teryg ii
S u bfilu m : V ertebra ta
O rdo : G o norynch iform e s
Fa m ili : C h a nid a e
G e nu s : La cé pè de, 1803
S pe sies : C h a nos ch a nos
N am a um u m :

3 .1 .2 C iri – c iri ik a n B a nd en g
Ik a n b an d en g b e n tu k tu b u h n ya ra m p in g , m u lu t term in a l, tip e s is ik
c yc lo id , J a ri – ja ri s em u a n ya lu n ak , ju m la h s irip p u n g g u n g a n ta ra 1 3 – 1 7 ,
s irip a n a l 9 – 1 1 , s irip p e ru t 1 1 – 1 2 , s irip e k o rn ya p a n ja n g d a n b e rc a g a k ,
ju m la h s is ik p a d a g u ra t s is i a d a 7 5 – 8 0 k e p in g , p a n ja n g m a k s im u m 1 ,7 in ,
b ia s a n ya 1 ,0 in (M o yle & C e c h , 2 0 1 0 ).
M e n u rut (T , 2 0 0 6 ), ik a n b a n d en g m e m p u n ya i tu b u h
ya n g ra m p in g d a n d itu tu p i o leh s is ik d en g a n ja ri-ja ri ya n g lun a k . S irip ek o r
ya n g p a n ja n g d a n b e rc a g a k . M u lu t s e d a n g d a n n o n p ro tra c tile d en g a n
p o s is i m u lu t s a tu g a ris d en g a n s is i b a w a h b o la m a ta d a n tid a k

7
m e m ilik is u n g u t. Ik a n in i m e m ilik i tu b u h la n g s in g d en g a n s irip ek o rn ya
b e rc a b a n g s e h in g g a m a m p u b eren a n g d en g a n c ep at. W a rn a tu b u h n ya p u tih
k ep era k – p era k a n . m u lu t tid a k b erg e rig i s eh in g g a m e ny uk a i m a k a n a n
g a n g g a n g b iru ya n g tu m b u h d i d a s a r p era ira n (h erb iv o ra ).

3.2 Deskripsi Ikan Kembung

Ik a n k e m b u n g d ik e n a l s e b a g a i m a c k a relfis h ya n g term a s u k ik a n
ek o n o m is p e n tin g d a n p o ten s i ta n g k a p a n ya n a ik tia p ta h un n ya . Ik a n in i
m e m ilik i ra s a c u k u p e n a k d a n g u rih s e h in g g a b a n ya k d ig e m a ri o le h
m a s ya ra k a t. M en u ru t B a d a n K e ta h a n a n P an g a n P ro v ins i D IY (2 0 1 3 ),
k o m p o s is i g izi ik a n k e m b u n g c uk u p tin g g i, ya k n i s e tia p 1 0 0 g ra m d a g in g
ik a n k e m b u n g m en g a n d u n g a ir 7 6 % , p ro te in 2 2 g , le m a k 1 g , k a ls iu m 2 0 m g ,
p o s p o r 2 0 0 m g , b es i 1 g , v ita m in A 3 0 S I d a n v ita m in B 1 0 ,0 5 m g .
P o ten s i ik a n k em b u n g d i In d o ne s ia s a ng a t b es a r. M en u ru t
K em en teria n K ela u ta n d a n Pe ik a n a n ( 2 0 1 2 ) ju m la h ta n g k a p a n ik a n
k em b u n g di In d o n es ia m e n c a p a i 2 1 4 .3 8 7 -2 9 1 .8 6 3 to n (ta h u n
2 0 0 1 -2 0 1 1 ).D i In d o n es ia k h u s u s n ya d a era h P an ta i U ta ra J a w a ik a n
k em b u n g d io la h m e n ja d i p ro d u k fe rm en ta s i b e ru p a ik a n p e d a ya n g
m e m p u n ya c ita ra s a d a n a ro m a k h a s s erta m e m p u n ya i d a ya a w et. M en u ru t
K em en teria n P erik a n a n d a n K ela u ta n (2 0 1 2 ), p ro d u k s i ik a n p e d a d ari ta h u n
2 0 0 1 -2 0 1 1 m en c a p a is eb es a r 1 3 .4 2 4 -3 .8 4 8 to n .

3 .2 .1 K la s ifik a s i Ik a n K em b u n g
Ik a n k e m b u n g a tau s erin g k a li d is eb u t ind ia n m a c k ere l
ik a n k em b u n g la k i-la k i d a n ik a n
k em b u n g p e re m p u a n te rm a s u k ik a n p e lag is k ec il ya n g m e m ilik i n ila i
ek o n o m is m e n en g a h, s eh in g g a terh itu n g s eb a g a i k o m o d ita s ya n g c u k u p
p e n tin g b a g i p erik a n a n ta n g k a p . Ik a n k em b u n g m e m ilik i p a n ja n g m a k s im a l
3 5 c m TL . T erm a s u k ik a n p e la g is d i zo n a n eritik , o s e a n o d ro m . S w im m in g
la yer b e rk is a r a n ta ra 2 0 – 9 0 m . P en ye b a ra n te rb a n ya k d i S a m u d era H in d ia
d a n s eb a g ia n P a s ifik T im u r (La ra s a ti, 2 0 1 1 ).
Ik a n in i m e ru p a k a n je n is s c h o o lin g fis h a ta u ik a n ya n g b erg e ro m b o l.
Ik a n in i b ere n a n g d e n g a n c a ra m u lu t d a n ta p is in s a n g terb u k a . In i
m e ru p a k a n c a ra ik a n in i m a k a n d en g a n m en ya rin g p la nk to n ya n g m a s u k k e
m u lu t d a n te rs arin g d i ta p is in s a n g . P a n ja n g tu b u h m a k s im a l ik a n k e m b u n g
b ia s m e n c a p a i 3 5 c m .

K ingd om : A nim alia

Filum : C h o rda ta
K elas : P isc es
S u bke la s : T e leo stei
O rdo : P ercom o rpy
S u b o rdo : S c om brida e
Fa m ili : S c om brida e

8
 G e nu s : R ast rellige r
S pe sies la ki-la ki : R ast rellige r ka na gu rta
S pe sies pere m pu a n : R ast rellige r bra c h ysom a
N am a um u m : India n m a cke rel (Inggris),
kem b ung (Indo nesia )

3 .2 .2 C iri-c iri Ik a n K e m b u n g
Ik a n k e m b u n g lela k i m e m ilik i c iri-c iri te rd a p a t d u a s irip p u n g g u n g
s ec a ra terp is a h ya n g m a s in g -m a s in g terd iri d a ri 8 h in g g a 9 ja ri-ja ri le m a h .
S irip d a d a te rd iri d a ri 1 6 hin g g a 1 9 ja ri-ja ri s irip le m a h, s irip p e ru t te rd iri d a ri
7 h in g g a 8 ja ri-ja ri le m a h , s irip ek o r te rd iri d ari 5 0 h in g g a 5 2 ja ri-ja ri le m a h
b e rc a b a n g d a n s is ik p a d a g a ris ru s u k (lin e a la tera lis ) terd iri d a ri 1 2 7 h in g g a
1 3 0 b u a h s is ik . S e la in itu , ik a n in i m em ilik i p a n ja n g to ta l 3 ,4 s a m p a i 3 ,8 k a li
tin g g i b a d a n d a n p a n ja n g k ep a la le b ih d a ri tin g g i k ep a la .
Ik a n k em b u n g p ere m p u a n m em ilik i b e n tuk tu b u h p ip ih d en g a n
b a g ia n d a d a le b ih b e s a r d a rip a d a b a g ia n tu b u h ya n g la in d a n d itu tu p i o le h
s is ik ya n g b eru k u ra n k e c il d a n tid a k m u d a h lep as . W a rn a tu b u h b iru
k eh ija u a n d i b ag ia n p u n g g u n g d e n g a n titik g e la p a ta u to to l-to to l h ita m d i
a ta s g a ris ru s u k s e d a n g k a n b ag ia n b a w ah tu b u h b e rw a rn a p u tih p era k . S irip
p u n g g u n g (d o rs a l) te rp is a h n ya ta m e n ja d i d u a b u a h s irip , m a s in g -m a s in g
te rd iri a ta s 1 0 h in g g a 1 1 ja ri-ja ri k e ra s d a n 1 2 h in g g a 1 3 ja ri-ja ri le m a h (
D irek to ra t J en d ra l Pe rik a n a n , 1 9 7 9 ). S irip d u b u r (a n a l) b e rja ri-ja ri lem a h 1 2 .
D i b e la k a n g s irip p u n g g u n g k ed u a d a n s irip d u b u r terd a p a t 5 s a m p a i 6 s irip
ta m b ah a n ya n g d is eb u t fin let. S irip p eru t (v en tra l) terd iri d ari 1 ja ri-jari k e ra s
d a n 5 ja ri-ja ri le m a h . S irip ek o r (c a u d al) b erc ag a k d a la m d a n s irip d a d a
(p e c to ra l) leb a r d a n m eru n c in g .

3.3
B a k te ri E s c h eric h ia c o li m e rup a k a n s p es ie s d en g a n h a b ita t a la m i
d a la m s a lu ra n p e n c e rn aa n m a n u s ia m a u p u n h ew a n . B a k teri in i b erb e n tu k
b a ta n g , b eru k u ra n 0 ,4 - 0 ,7 x 1 ,0 -3 ,0 µ m , te rm a s u k g ra m n eg a tiv e, d a p a t
h id u p s ec a ra s o lite r m a u p u n k e lo m p o k , u m u m ny a m o til, tid a k m em b e n tu k
s p o ra , s e rta fa k u lta tif a n a ero b . (C a rter & W is e 2 0 0 4 ). M e to d e u ji ya n g b is a
d ig u n a k a n S N I 2 3 3 2 .1 :2 0 1 5 C a ra u ji M ik ro b io lo g i B a g ia n 1 P e n en tu a n
K o lifo rm d a n E s c h e ric h ia c o li P a d a P ro d u k Pe rik a n a n .

9
3.4 Coliform
B a k te ri c o lifo rm a d a la h m ik ro o rg a n is m e ya n g m e n ja d i in d ik a to r
s a n ita s i k u ra n g b a ik a ta u a d a n ya p e n c e m a ra n lin g k u n g a n b a k teri c o lifo rm
te rg o lo n g d a la m fa m ili E nte ro b a c te ria c ea e ya n g m e m b ed a k an 2 k e lo m p o k
ya itu k elo m p o k n o n fek a l d a n fek a l, C o lifo rm fek a l m e ru p a k a n b a k teri
in d ik a to r ya n g m e n ja d i ta n d a a d a tid ak n ya p en c em a ra n b a k te ri p a to g en ,
c o lifo rm ju g a m eru p ak a n b a k te ri g ra m n e g a tif b e rb en tu k b a ta ng ya n g d a p a t
m e n g h a s ilk a n g a s ( H a lim a h , L. K . 2 0 0 8 ). . M e to d e u ji ya n g b is a d ig u n a k a n
S N I 2 3 3 2 .1 :2 0 1 5 C ara u ji M ik ro b io lo g i B a g ia n 1 P e ne n tu a n C o lifo rm d a n
E s c h eric h ia c o li P a d a Pro d u k P erik a n a n . E s c h eric h ia c o li m e ru p a k a n
k elo m p o k b a k te ri ya n g d ig u n a k a n s eb a g a i in d ik a to r a d a n ya p o lu s i k o to ra n
d a n k o n d is i s a n ita s i ya n g tid a k b a ik .

10
 4. METODE PRAKTIK

4.1 Waktu dan Tempat

P ra k tik m a g a n g d ila k s a n a k a n d i L a b o ra to riu m S ta s iu n K a ra n tin a Ik a n
P en g en d a lia n M u tu d a n K e a m a n a n H a s il P e rik a n a n B a n d u n g d im u la i
s eja k ta n g g a l 0 9 O k to b er 2 0 2 3 s a m p a i 0 9 D e s em b e r 2 0 2 3 . La b o rito riu m
te rs eb u t d ik la s ifik as ik a n s eb a g a i la b o ra to riu m k ela s II, d i m a n a
p e n g e k ela s a n te rs e b u t b e rd a s a rk a n fa s ilita s s erta k a p a s ita s ya n g d i m ilik i
o leh la b o ra to riu m d i S K IP M B an d u n g . A d a p u n je n is la b o ra to riu m ya ng a d a :
La b o ra to riu m M ik ro b io lo g i M u tu , M ik ro b io lo g i H P IK , p a ra s it, v iru s , d a n
J a m u r, o rg a n o lep tik , k im ia (fo rm a lin ).

4.2 Metode Pengambilan Data

P en g a m b ila n d a ta p a d a p ra k tik m a g a n g d i L a b o ra to riu m M ik ro b io lo g i
M u tu S ta s iu n K a ra n tin a Ik a n P en g en d a lia n M u tu d a n K e a m a n a n H a s il
P erik a n an m elip u ti d a ta p rim er d a n d a ta s ek u n d e r.

4 . 2 .1 D a ta Prim e r
ad a la h d a ta-d a ta ya n g d ia m b il la n g s u n g , d ip ero le h d a ri s u m b e rn ya
d en g a n m e n g g u n a k a n c ara :
● Pe n g a m a tan d a ta u n tu k p e n g u m p u la n d a ta d e n g a n m elih a t
o b jek -o b jek ya n g d iteliti s ec a ra la n g s u n g , m is a ln ya d a ta d ip ero le h
s ec a ra b e n a r d a n n ya ta .
● W a w a n c a ra d ila k u k a n a ta u d ig u n a k a n un tu k m e n g u m p u lk a n d a ta
d en g a n m en g a ju k a n p e rta n ya a n s e c a ra la n g s u n g p a d a n a ra s u m b e r
s eh in g g a d id a p a tk a n ja w a b a n ya n g len g k ap .
● E k s p e rim en m e to d e in i d ila k u k a n d e n g a n c a ra m e la k uk a n p e rc o b a a n
d a n p e m e rik s a a n te rh a d a p s a m p e l ya n g d ian a lis is .
● Pa rtis ip a s if m e to d e in i d ila k u k a n d e n g a n c a ra m e la k s a n a k a n p ro s e s
p en g u jia n s etia p ta h a p d en g a n k e telitia n d a n p e m a h a m a n ya ng b a ik .

4 . 2 .2 D a ta Se k u n d e r
ad a la h d a ta ya n g d ip e ro leh d a ri o ra n g la in a ta u s um b er s ek u n d er d a n
te la h ters u s u n d a la m b en tu k d o k u m en .

4.2 Sterilisasi Ruangan, Alat dan Media

4 .2 .1 S trelis a s i R ua n g a n
P rin s ip s terilis a s i ru a n g a n m en g h ila n g k a n s e lu ru h m ik ro o rg a n is m e ya n g
te rd a p a t p a d a p era n g k a t la b o ra to riu m , d ila k u k a n s etia p h a ri, u n tu k
m e m a s tik a n ru a n g a n teta p d a lam k e a d a a n s teril s eh in g g a tid a k
m e n g g a n g g u p a d a s a a t p e n g u jia n b erla ng s u n g . Ste rilis a s i d en g a n
m e n g g u n a k a n la m p u U V ya n g d is ed ia k an tid a k h a n ya d a la m la m in a ry a ir
flo w te tap i d i s e tia p ru a n g p en g u jia n ya n g d i p a s a n g d ib ag ia n d a ta ra n lan g it-
la ng it d a n a k a n d i h id u p k a n s ete la h ru a n g a n tela h d ib ers ih k a n d en g a n c a ra
m a n u a l a g a r s terilis a s in ya s em p u rn a a ta u m em u a s k a n d a la m h a l s teril.
J ik a s terilis a s i b erla n g s u n g s ec a ra s em p u rn a , m a k a s p o ra b a k teri ya n g
m e ru p a k a n b e n tu k p a lin g res is ten d a ri k e h id u p a n m ik ro b a a k a n d ilu lu h k a n

11
 4 .2 .3 S te rilis a s i A la t
S te rilis a s i a la t s ec a ra b a s a h d ila k u k a n d en g a n a u to c la ve a ta u ya n g
b ia s a d is eb u t p em u s n a h a n s e te la h a la t d ig u n a k a n u n tu k p e n g u jia n . M eto d e
in i d ig u n a k a n u n tu k m en s terilk an m e d ia , a q u a d e s d a n a la t-a la t ya n g te rb u a t
d a ri p la s tik d im a s u k k a n k ed a la m a u to c lav e d a n s u h u ya n g d ig u n a k a n s u h u
1 2 1 °C d en g a n te k an a n a tm s ela m a 1 5 m en it. P erh itu n g a n w ak tu 1 5 m en it
d i m u la i s e m e n ja k term o m eter p a d a a u to c la v e m e n u nju k 1 2 1 °C , p e nu tu p
a u to c la v e d a p a t d ib u k a s e tela h term o m e ter m e n u n ju k k a n a n g k a n o l, d a n
te k a n a n a uto c la ve s u d a h tu ru n h in g g a 0 a tm .
S te rilis a s i a la t s ec a ra k erin g d ila k u k a n d e n g a n m en g g u n a k a n o v en ,
s te rilis a s i d e n g a n c a ra in i d ite ra p k a n p a d a b en d a a ta u a la t ya n g ta h a n
te rh a d a p s u h u tin g g i s e p e rti c a w a n p e tri, ta b un g rea k s i, ta b u n g d u rh a m ,
g e la s u k u r, erlem eye r d ll. S e b e lu m a la t ters eb u t d i o v en d i la k u k a n s te rilis a s i
b a s a h d a la m a u to c la v e, d i la k u k a n p e n c u c ia n d e n g a n m en g g u n a d e terje n
d a n d i b ila s d e n g a n m en g g u n a k a n a ir b ers ih , s e te la h b e rs ih d a n k erin g a la t
s u d a h b is a d i m a s u k k a n k ed a la m o ve n , s e b e lum d i o v en s e m u a a la t d i
b u n g k u s d en g a n k erta s , la lu d im a s u k a n p a d a o ve n d en g a n s u h u 1 6 5 °C
s ela m a ± 1 ja m .

4 .2 .4 S tre lis is a s i M ed ia
D ila k u k a n m en g g u n a k a n a u to c la v e d e n g a n s u h u 1 2 1 ºC d a n tek a n a n
1 A T M s ela m a 1 5 m en it.

4.3 Alat, Bahan dan Pereaksi

Nama Alat Fungsi Gambar

A u to c la v e S eb a g a i a la t s terilis a s i
m e d ia , re a g e n t, dan
a n ek a p e ra la ta n
g la s s w a re la in n ya , s erta
u n tu k m em b unuh
m ik ro o rg a n is m e

B a ll P ip et
La m in a ry A ir F lo w
U n tu k m em in d a h k a n
s eju m la h v o lu m e la ru ta n
U n tu k m e n ja g a a la t d a n
m e d ia ya n g d ig u n a k a n
d a la m p ro s e s p en g u jia n
s eh in g g a tid a k
m e n yeb a b k a n
k o n ta m in a s i

12
O ve n S eb a g a i a la t s terilis a s i
k erin g u n tu k p e ra la ta n
g la s s w a re

C a w a n p etri S eb a g a i te m p a t a ta u
wad ah pe num b uhan se l
a ta u m ik ro o rg a n is m e

Ta b u n g rea k s i B erfu n g s i un tu k w a d a h
la ru ta n /m ed ia ya n g
m enahan p e ru b a h a n
te m p era tu r d an ta h a n
te rh a d a p rea k s i k im ia

Bunse n U n tu k m em a n a s k a n
a ta u m en g h in d a ri
te rja d in ya k o n ta m in a n

O s e lo o p U n tu k m en g a m b il
b a k teri ya n g a k an
d itan a m p a d a s u a tu
m e d ia a g a r.

13
In k u b a to r U n tu k
m e n g e m b a n g b ia k a n
o rg a n is m e s el, b aik
u n is e lu le r m a u p u n
m u ltis e lu le r

N e rac a a n a litik U n tu k m en g u k u r b era t

s u a tu za t a tau b a h a n
k im ia d a la m ju m la h k e c il

E rle n m eye r U n tu k m en c a m p u r
b e rb a g a i b a h a n
k o m p o s is i m ed ia
H o t p la te U n tu k m ela k u k a n
pengadukan dengan
m e n g g u n ak a n s tirrer b a r
ya n g d i c e lu p k a n p a d a
s a m p el
M a g ic s tirre r U n tu k m en g a d u k a ta u
m e n c a m p u rk a n la ru ta n
agar ho m o gen.

14
Sto m a c er U n tu k m en g h a lu s k a n
s a m p el

Pip et U k u r U n tu k m g a m b il m e d ia
c a ir s e s u a i u k u ra n
W a te r B a th U n tu k m en c ip ta k a n s u h u
ya n g k o n s ta n d a n u n tu k
m e n g in k u b a s i p a d a
a n a lis is m ik ro b io lo g i
K a c a Pre p a ra t M e n ja d i tem p a t o b jek
ya n g a k a n d ia m a ti.
Sh o w C a s e U n tu k m en yim p a n
m e d ia
R a k T a b u n g R ea k s i U n tu k m en yim p a n
ta b u n g rea k s i a g a r teta p
b e ra d a p a d a p o s is i
b e rd iri
B a ll P ip et U n tu k m em in d a h k a n
s eju m la h v o lu m e la ru ta n
Pis a u U n tu k m em b ed a h ik a n
p a d a s a a t p rep a ra s i
sam p e
M ik ro P ip et U n tu k m em in d a h k a n
c a iran d a la m u k u ra n
k ec i
Sp a tu la / s en d o k la b U n tu k M en g a m b il s u a tu
s a m p el a ta u b a h a n
p e n elitia n
A lu m u n iu m F o il B erfu n g s i s eb a g a i
p e n utu p b a g ia n m u lu t
a la t-a la t p ra k tik u m
b e rb a h a n k a c a s e h in g g a
b a k teri a ta u p u n ja m u r
tid ak a k a n m as u k k e
d a la m n y

15
4.4 Metode Pembuatan Media Uji
S em u a m e to d e p en g u jia n ya n g a d a d i te m p a t m a g a n g

16
 5. HASIL DAN PEMBAHASAN

5.1. Uji
P em erik s a a n s e c a ra m ik ro b io lo g i p a d a k e d u a s a m p el k o n s u m s i
ya itu : ik a n b a n d en g d a n ik a n k e m b u n g
ya n g b ertu ju a n u n tu k m en g eta h u i k eb e ra d a a n b a k te ri pad a
k ed u a s a m p e l ik a n k o n s u m s i. K ed u a S a m p e l te rs e b u t d id a p a tk a n la n g s u n g
d a ri p a s a r c im in d i k e c a m a ta n C im a h i T en g a h , K o ta C im a hi, Ja w a B a ra t.
S a m p el k e m u d ia n d i p rep a ras i d a n d ia m b il m a s in g m a s in g s e b a n ya k 2 5
g ra m .
5.2 Uji
5.3 dst....

17
 6. KESIMPULAN DAN SARAN
6.1 Kesimpulan
6.2 Saran

18
 DAFTAR PUSTAKA

D IY , B . k . (2 0 1 2 ). D a ta k a nd u n g a n g iz i b a h a n p a n g a n d a n h a s il o la h a n n ya .
J a y, J . M . ( 1 9 7 8 ) . M o d e rn fo o d m ic ro b io lo g y, s ec o n d e d itio n .
M o yle, P . B ., & C e c h , J . J . (2 0 1 0 ). F is h e s A n In tro d u c tio n to Ic h th yo lo g y,
fo u rth e d itio n .
N u ra e n i, K . Y . (2 0 0 0 ). M ik ro b io lo g i p a n g a n d a n p e n g o la h a n .
N u rjan a h , S e tya n in g n ih , I. , S u k a rn o , & M u ld a n i, M . ( 2 0 0 4 ) . K e m u n d u ra n
M u tu Ik a n N ila M e ra h (O reo c h ro m is s p . )S ela m a P en yim p a n a n P a d a
Suhu R uang. (V II), 3 7 -4 3 .
p e rik a n a n , K . k . ( 2 0 1 2 ). S ta tis tik p erik a n a n ta n g k a p in d o n es ia ta h u n 2 0 1 1 ,
D irek to ra t jen d e ra l p erik a n a n ta n g k a p .
P ra ta m a , R . I., S u m a rya n to , H ., S an to s o , J ., & Z a h iru d in , W . (2 0 1 2 ).
K A R A K T E R IS T IK S E N S O R I B E B E R A P A P R O D U K IK A N A S A P .
(7 ), 1 1 7 -1 3 0 .
T , D . (2 0 0 6 ). T a k s o n o m i, M o rfo lo g i, D an Is tila h Is tila h T e k n ik P erik a n a n .
.
W .P a m ijia ti. (2 0 0 9 ). P en g aru h E k s tra k D a un S ela s ih (O c im u m b a s ilic u m
Lin n ) T erh a d a p M u tu K e s eg a ra n Ik a n B a n d e n g S ela m a P e n yim p a na n
D in g in (C h a n o s -c h a n o s F o rs k ).

19
 LAMPIRAN

F o to 2 k eg ia ta n p e n g u jia n d i tem p a t m a g a n g

C o n to h le m b a r h a s il u ji s e tia p p en g u jia n

D a fta r k e h a d ira n

20
 DAFTAR PUSTAKA

(u .d .).
D IY , B . k . (2 0 1 2 ). D a ta k a nd u n g a n g iz i b a h a n p a n g a n d a n h a s il o la h a n n ya .
J a y, J . M . ( 1 9 7 8 ) . M o d e rn fo o d m ic ro b io lo g y, s ec o n d e d itio n .
M o yle, P . B ., & C e c h , J . J . (2 0 1 0 ). F is h e s A n In tro d u c tio n to Ic h th yo lo g y,
fo u rth e d itio n .
N u ra e n i, K . Y . (2 0 0 0 ). M ik ro b io lo g i p a n g a n d a n p e n g o la h a n .
N u rjan a h , S e tya n in g n ih , I. , S u k a rn o , & M u ld a n i, M . ( 2 0 0 4 ) . K e m u n d u ra n
M u tu Ik a n N ila M e ra h (O reo c h ro m is s p . )S ela m a P en yim p a n a n P a d a
Suhu R uang. (V II), 3 7 -4 3 .
p e rik a n a n , K . k . ( 2 0 1 2 ). S ta tis tik p erik a n a n ta n g k a p in d o n es ia ta h u n 2 0 1 1 ,
D irek to ra t jen d e ra l p erik a n a n ta n g k a p .
P ra ta m a , R . I., S u m a rya n to , H ., S an to s o , J ., & Z a h iru d in , W . (2 0 1 2 ).
K A R A K T E R IS T IK S E N S O R I B E B E R A P A P R O D U K IK A N A S A P .
(7 ), 1 1 7 -1 3 0 .
T , D . (2 0 0 6 ). T a k s o n o m i, M o rfo lo g i, D an Is tila h Is tila h T e k n ik P erik a n a n .
.
W .P a m ijia ti. (2 0 0 9 ). P en g aru h E k s tra k D a un S ela s ih (O c im u m b a s ilic u m
Lin n ) T erh a d a p M u tu K e s eg a ra n Ik a n B a n d e n g S ela m a P e n yim p a na n
D in g in (C h a n o s -c h a n o s F o rs k ).
 DAFTAR LAMPIRAN

1 . S c o re s h ee t P e n g u jia n O rg a n o le p tik (S N I/ s c o res h e et ya n g a d a d i

U P I)
2 . T a b e l d a ta s u p p lie r
3 . T a b e l Pe n g a m a tan suhu produk p a d a s e tia p ta h a p an p ro d u k s i ( 2 0
k a li p e n g a m a ta n )
4 . T a b e l P en g a m a ta n suhu air p a d a s etiap ta h a p a n p ro d u k s i (2 0 k a li
p e n g a m a ta n )
5 . T a b e l Pe n g a m a ta n suhu ruangan d i U PI (s es u a ik a n d en g a n
p e m b a g ia n ru a n g p ro d u k s i) (2 0 k a li p en g a m a ta n )
6 . T a b e l p en g a m a ta n rendemen (p ilih ta h a p a n p ro s e s n ya )
7 . T a b e l P en g a m a ta n G M P
8 . T a b e l P en g a m a ta n S S O P
9. Data Pengujian ALT
1 0 . K u es io n e r S K P
1 1 . D a ta n a m a d a n a s a l b a h a n b a k u d a n v o lu m e p en e rim a a n
bahan baku
1 2 . S ertifik a t S K P , H A C C P U P I
1 3 . D a ta La y o u t U P I