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KARAGENAN
LAPORAN RESMI PRAKTIKUM
TEKNOLOGI HASIL LAUT

Disusun Oleh :
Nama : Paulus Yosef Su!a Pima An"i#a
NIM : $%&'0&00$(
Kelom)o# E*

PROGRAM STUDI TEKNOLOGI PANGAN
+AKULTAS TEKNOLOGI PERTANIAN
UNI,ERSITAS KATOLIK SOEGI-APRANATA
SEMARANG
%0$(
1. HASIL PENGAMATAN
Hasil )en.ama/an #aa.enan "a)a/ "iliha/ )a"a /a0el $ 0ei#u/ ini&
Ta0el $& Hasil Pen.ama/an Kaa.enan
Kelom)o# 1ea/ A2al 3.am4 1ea/ A#hi 3.am4 5 Ren"emen
E$ (0 $6700 (6000
E% (0 $6700 (6000
E* (0 %6%00 86800
E( (0 $6800 *6'80
E8 (0 %6$00 86%80
1e"asa#an /a0el hasil )en.ama/an6 0ea/ a2al #aa.enan !an. "i.una#an oleh semua
#elom)o# a"alah 80 .am& Pa"a #elom)o# E$ "an E% "en.an 0ea/ a#hi $67 .am6
"i"a)a/#an 0ea/ en"emen se0esa (5& Selan9u/n!a #elom)o# E* "en.an 0ea/ a#hi
%6% .am6 "i"a)a/#an 0ea/ en"emen se0esa 8685& Kelom)o# E( "an E8 0e/uu/:/uu/
men"a)a/#an 0ea/ a#hi $68 .am "an %6$ .am6 "i"a)a/#an 0ea/ en"emen se0esa
*6'85 "an 86%85&
$
1. PEMBAHASAN
Rum)u/ lau/ meu)a#an .olon.an al.a6 !ai/u /um0uhan 0e#loofil !an. /e"ii "ai sa/u
a/au 0an!a# sel "an 0e#oloni 3Afian/o ; Li<ia2a/!6 $==*4& Rum)u/ lau/ 3seaweed4
meu)a#an /um0uhan /in.#a/ en"ah 0eu)a /hallus 30a/an.4 !an. 0e>a0an.6 hi"u) "i
lau/ "an /am0a# "en.an #e"alaman !an. masih "a)a/ "i>a)ai oleh >aha!a ma/ahai
3Nin"!anin.6 %00'4& Rum)u/ lau/ meu)a#an 0ahan 0a#u "ai 0e0a.ai 9enis )o"u#
olahan 0enilai e#onomi /in..i6 um)u/ lau/ selain "i.una#an se0a.ai )e2ana ma#anan
"an /e#s/i#6 9u.a "a)a/ "i.una#an se0a.ai )o"u# )an.an mau)un non )an.an6 se)e/i
a.a:a.a6 #aa.inan6 "an al.ina/& Se0a.ai sum0e .i?i6 um)u/ lau/ memili#i #an"un.an
#a0ohi"aa/6 )o/ein6 se"i#i/ lema#6 "an a0u 3na/ium6 #alium6 fosfo6 na/ium6 0esi6
!o"ium4& Kan"un.an )o/ein )a"a um)u/ lau/ a"alah (:%85 "ai 0ea/ #ein.n!a 3Insan
"an @i"!a/ini6 %00$4&
Kaa.enan meu)a#an )olisa#ai"a !an. men.alami e#s/a#si "ai um)u/ lau/&
Kaa.enan meu)a#an susunan "ai .ala#/an /esulfa/asi linea hi"ofili# "en.an uni/
.ala#/osa "an 3,6-anhydro galactose Aam)o et al&6 3%00=4& Kaa.enan se>aa #omesial
a"a * 9enis !ai/u io/a6 #a))a6 "an lam0"a&
Pa"a )a#/i#um Te#nolo.i Hasil Lau/ #ai ini6 "ila#u#an )em0ua/an #aa.enan
men..una#an me/o"e e#s/a#si #aa.enan& Mula:mula um)u/ lau/ "i/im0an. (0 .am&
Lalu um)u/ lau/ "i)o/on. #e>il "an "i 0len"e& Rum)u/ lau/ "i)o/on. 0e/u9uan un/u#
mem)eluas )emu#aan "an mem)e0esa #on/a# 3A)ilia6 %0074& Se/elah i/u6 um)u/
lau/ "ie0us "alam ai 800 ml selama $ 9am "en.an suhu B0A : =0A& Kemu"ian6 )H
lau/an "ia/u men9a"i )H B "en.an )enam0ahan HAl 06$N a/au NaOH 06$N& Se/elah
i/u6 hasil e#s/a#si "isain. "an >aian fil/a/ "i/am)un. "alam 2a"ah& Hal ini sesuai
"en.an /eoi "ai Klose ; Gli>#sman 3$='84 0ah2a )oses )emanasan "a)a/
mema#simal#an #aa.enan !an. 0isa "ilau/#an& Poses )en!ain.an ini 0efun.si un/u#
memisah#an fil/a/ "ai am)as& Aaian fil/a/ "i/am0ah NaAl $05 se0an!a# 85 <olume
fil/a/ "an "i)anas#an "en.an suhu 70A& +un.si "ai NaAl menuu/ Aam)o et al&6
3%00=4 a"alah )em0en/u# en"a)an6 "imana NaAl meu)a#an .aam !an. 0esifa/
thermo-reversible. Kemu"ian fil/a/ "i/uan. #e 2a"ah 0eisi *00 ml >aian IPA& Aaian
%
*
IPA menuu/ +ai"li!ah 3%0$04 0efun.si un/u# men.en"a)#an #aa.enan& +il/a/ ini
#emu"ian "ia"u# selama $0:$8 meni/ sehin..a /e0en/u# en"a)an #aa.enan "an se.ea
"i/iis#an en"a)an /ese0u/& Lalu "ien"am #e "alam >aian IPA sam)ai mem)eoleh
sea/ #aa.enan& Sea/ #aa.enan "ile/a##an "i 2a"ah /ahan )anas un/u# "i#ein.#an #e
"alam o<en "en.an suhu *0A selama semalam& Se/elah "i o<en6 sea/ #ein. "i/im0an.
"an "i 0len"e men9a"i /e)un. #aa.enan& Menuu/ @inano et al& 3$=B046 )oses
)en.ein.an 0e/u9uan un/u# men.elua#an a/au men.hilan.#an se0a.ian ai "ai sua/u
0ahan )a"a/ "en.an >aa men.ua)#an ai "an mem0uan. ua) !an. /e0en/u#
men..una#an ene.i )anas& +a#/o !an. mem)en.auhi )en.ein.an a"alah luas
)emu#aan6 suhu )en.ein.an6 alian u"aa6 "an /e#anan ua) "i u"aa&
1e"asa#an hasil )en.ama/an )a"a #elom)o# E$ "an E% "en.an 0ea/ a#hi $67 .am6
"i"a)a/#an 0ea/ en"emen se0esa (5& Selan9u/n!a #elom)o# E* "en.an 0ea/ a#hi
%6% .am6 "i"a)a/#an 0ea/ en"emen se0esa 8685& Kelom)o# E( "an E8 0e/uu/:/uu/
men"a)a/#an 0ea/ a#hi $68 .am "an %6$ .am6 "i"a)a/#an 0ea/ en"emen se0esa
*6'85 "an 86%85& Menuu/ Dis/an/ina et al& 3%00'4 0e0ea)a fa#/o !an.
mem)en.auhi hasil e#s/a#si a"alah 9enis )elau/6 asio 0ea/ 0ahan "en.an <olume
)elau/6 suhu6 )en.a"u#an6 2a#/u e#s/a#si6 "an )een"aman& Pe0e"aan hasil !an.
"i"a)a/#an 0e0e"a "a)a/ "ise0a0#an #aena #an"un.an #aa.enan )a"a se/ia) um)u/
lau/ 0e0e"a:0e"a& Selain i/u #esalahan "a)a/ 9u.a "ise0a0#an #aena suhu )emanasan
!an. 0e0e"a:0e"a& U#uan um)u/ lau/ saa/ )emo/on.an a2al 9u.a #uan. sea.am6
sehin..a hasiln!a men.alami )e0e"aan&
1e"asa#an 9unal CSynergistic effects between k-carrageenan and locust be an gum on
physicochemical properties of edible film made thereofD hasil mem0ei#an infomasi
!an. 0e.una /en/an. sifa/ s/u#/ual #:#aa.enan "an L1G se/a )eu0ahan s/u#/ual
"alam film 9ain.an "iin"u#si #e/i#a asio )en>am)uan !an. 0e0e"a "ai "ua
)olisa#ai"a& Pen.auh sine.is an/aa #e"ua )olime "i9elas#an oleh #emam)uan
men>am)un!a an/aa #:#aa.enan "en.an L1G6 a"alah salah sa/u u/ama alasan un/u#
)e0e"aan "iama/i "alam film )o)e/i 3Ma/ins et al., %0$%4&
(
1e"asa#an 9unal CEffect of pectin and carrageenan on thermophysical and
rheological properties of tapioca starchD Penam0ahan h!"o>olloi"s un/u# /e)un.
/a)io#a mem0ei#an se0uah efe# !an. 0esa /eha"a) sifa/ eolo.i "ai >am)uan
hi"o#oloi" )a/i6 "an se9auh <aiasi ini /e.an/un. )a"a #onsen/asi "an 9enis
hi"o#oloi" !an. "i/am0ah#an& H!"o>olloi"s menin.#a/#an <is#osi/as )as/a /e)un.
/a)io#a selama )emanas6 se/a #oefisien #onsis/ensi "an <is#osi/as 9elas )a"a %8EA&
In/ea#si an/aa h!"o>olloi"s "an )a/i men.hasil#an se"i#i/ mo"ifi#asi "ai
.ela/inisa/ion DSA )aame/e 31a0i> et al&6 %0074&
1e"asa#an 9unal CModification of k-carrageenan by graft copolymeriation of
methacrylic acid! synthesis and applicationsD "a/a s)e#/os#o)i men.#onfimasi 0ah2a
.af/in. asam me/a#ila/ /elah /e9a"i )a"a .u.us hi"o#sil6 "an 9u.a mene.as#an
me#anisme !an. "isaan#an un/u# o#ulasi& Da/a analisis /emal menun9u##an 0ah2a
)olime "i>an.#o##an le0ih s/a0il "ai )olime6 men.in.a/ #e"ua le0ih /in..i a#hi
/em)ea/u "e#om)osisi "an in/e.al )ose"ual suhu "e#om)osisi )olime
"i>an.#o##an "i0an"in.#an "en.an su0s/a/ 3Ti)a/h! et al., %00=4&
1e"asa#an 9unal C"ptimiation of the e#traction of carrageenan from kappaphycus
alvareii using response surface methodologyD )eneli/ian ini 0e/u9uan un/u#
men.o)/imal#an me/o"e al/ena/if e#s/a#si #aa.inan /an)a )ela#uan al#ali
se0elumn!a "an e/anol )esi)i/asi men..una#an $esponse Surface Methodology
3RSM4& Dalam an.#a un/u# mem)e#enal#an ino<asi "alam lan.#ah isolasi6 a/omisasi
)en.ein.an "i.una#an men.uan.i 2a#/u un/u# men"a)a/#an >aa.eenan 0u0u#
#ein.& Efe# 2a#/u e#s/a#si "an suhu )a"a hasil6 #e#ua/an .el6 "an <is#osi/as !an.
"ie<aluasi& Selain i/u6 0ahan "ie#s/a#si "iseah#an #e)a"a analisis s/u#/u6 "en.an
s)e#/os#o)i infameah "an esonansi ma.ne/i# nu#lis)e#/os#o)i 3$H:NMR46 "an
analisis #om)osisi #imia 3@e00e et al&6 %0$%4&
1e"asa#an 9unal C%ilute iota- and kappa-carrageenan solutions with high viscosities
in high salinity brinesD "ia"a#an e<aluasi #aa#/eis/i# eolo.i io/a "an #a))a #aa.enan
"alam lau/an ai& ,is#osi/as san.a/ menin.#a/ "en.an menin.#a/n!a #onsen/asi
)olime a/au salini/as& Mono<alen NaF #a/ion !an. le0ih efe#/if "alam menin.#a/#an
8
<is#osi/as "ai "i<alen Aa%F #a/ion& Se0uah ai .aam !an. le0ih #om)le#s !an.
men.an"un. NaF6 M.%F "an Aa%F #a/ion 9u.a menun9u##an <is#osi/as /in..i "an
salini/as /in..i 3I.laue et al., %0$04&
2. KESIMPULAN
Rum)u/ lau/ 3seaweed4 meu)a#an /um0uhan /in.#a/ en"ah 0eu)a /hallus
30a/an.4 !an. 0e>a0an.&
Kaa.enan meu)a#an )olisa#ai"a !an. men.alami e#s/a#si "ai um)u/ lau/&
Kaa.enan se>aa #omesial a"a * 9enis !ai/u io/a6 #a))a6 "an lam0"a&
Poses )emanasan "a)a/ mema#simal#an #aa.enan !an. 0isa "ilau/#an&
+un.si "ai NaAl a"alah )em0en/u# en"a)an&
IPA 0efun.si un/u# men.en"a)#an #aa.enan&
Pen.ein.an 0e/u9uan un/u# men.elua#an a/au men.hilan.#an se0a.ian ai "ai
sua/u 0ahan )a"a/ "en.an >aa men.ua)#an ai "an mem0uan. ua)&
1e0ea)a fa#/o !an. mem)en.auhi hasil e#s/a#si a"alah 9enis )elau/6 asio 0ea/
0ahan "en.an <olume )elau/6 suhu6 )en.a"u#an6 2a#/u e#s/a#si6 "an )een"aman&
Semaan.6 $B Se)/em0e %0$( Men.e/ahui6
Asis/en Dosen:
: Ale/heia Han"o#o
: Ma.ae/ha Rani Kiana
Paulus Yosef Su!a P A
$%&'0&00$(
7
3. DAFTAR PUSTAKA
Afian/o6 E& "an E& Li<ia2a/!&3$==*4& 1u"i"a!a Rum)u/ Lau/ "an Aaa
Pen.elolaann!a&1ha/aa& -a#a/a&
A)ilia In"ah A&et al & 3%0074&Posi"in. Semina Nasional Te#ni# Kimia In"onesia6
Estraksi &aragenan dari $umput 'aut (enis Eucheuma )ottoni& Palem0an.&
1a0i>6 -uisla<&6 Da.o Su0ai>&6 Du"i>a A>#a&6 ,las/a Pili?o/a&6 Miela Ko)9a&6 Nela
Ne"i> Ti0an& 3%0074& Effect of pectin and carrageenan on thermophysical and
rheological properties of tapioca starch. *aculty of *ood +Echnology, ,niversity in
"si(ek. )roatia.
Aom)o ,L6 Ka2ano D+6 "a Sil<a - D16 Aa<aos)ho I6& 3%00=4&
Aaa.eenans:1iolo.i>al )o)e/ies6 >hemi>al mo"ifi>a/ions an" s/u>/ual anal!sis& A
e<ie2& )arbohydrate -olymers6 '' 3%46 )&$7':$B0&
Dis/an/ina6 S)eisaG +a"ilahG En"ah R& D!a/an/iG "an Enn! K& A/a/i& 3%00'4& Pen.auh
Rasio 1ea/ Rum)u/ Lau/:Pelau/ Teha"a) E#s/a#si Kaa.enan:Kaa.enan& ,ol& 7 No&
% -uli %00': 8*:8B&
+ai"li!ah Nilna M& 3%0$04& Pose"in. Semina Nasional Te#ni# Kimia Tin9auan
Kuali/as Kaa.inan "ai Eu>hema Ao//oni )a"a Pen..unaan Pelau/ "an @a#/u
E#s/a#si !an. 1e0e"a&
I.laue6 S/efan&6 Yon.fu @u&6 Pa/i># SHule&6 Yon.>hun TAn.&6 @illiam A& Go""a" III&
3%0$$4& Dilu/e io/a: an" #a))a:>aa.eenan solu/ions 2i/h hi.h <is>osi/ies in hi.h
salini/! 0ines&Aalifonia& Uni/e" S/a/es&
Insan6 A& L& "an D&S& @i"!a/ini&3%00$4& Ma#oal.ae&Pu2o#e/o&
Klose6 R& E& an" M& Gli#sman&3$='84& Gums "alam Han"0oo# of A""i/i<e&%
n"
e"i/ion&
Ohio&
Ma/ins6 -oana T&6 Mi.uel A& AeHueia&6 Ana I& 1ou0on&6 Ana A& Pinheio&6 1a/olomeu
@& S& Sou?a&6 An/onio A& ,in>en/e& 3%0$%4& Synergistic effects between k-carrageenan
and locust be an gum on physicochemical properties of edible film made thereof&
Ins/i/u/e of 1io/e>hnolo.! an" 1ioen.ineein.6 Uni<esi"a"e "o Minho& Po/u.al&
Ti)a/h!6 -asas2ini&6 Dinesh Kuma Misha&6 Mi/hilesh Ya"a<&6 A)i/ San"&6 Kun9
1ehai& 3%00=4& Modification of k-carrageenan by graft copolymeriation of methacrylic
acid! synthesis and applications& Pol!me S>ien>e Resea>h La0oa/o!6 De)a/men/ of
'
B
AHemis/!6 Uni<esi/! of Allaha0a"& In"ia&
@e00e6 ,anessa&6 Sa0ina Ma/os "e Aa<alho&6 PAul -ose O.liai&6 Leila Ha!ashi&6
Pe"o Lui? MAniHue 1Ae/o& 3%0$%4& "ptimiation of the e#traction of carrageenan
from kappaphycus alvareii using response surface methodology&
@inano6 +&G&G S& +a"ia?G "an D& +a"ia?& 3$=B04& Pen.an/a Te#nolo.i Pan.an&
Game"ia6 -a#a/a&
5. LAMPIRAN
5.1. Perhitungn
Ren"emen a.a
Ke/ean.an : 0ea/ 0asah I (0 .am
Ke!"#$"% E1
Ren"emen a.a
Ke!"#$"% E2
Ren"emen a.a
Ke!"#$"% E3
Ren"emen a.a
Ke!"#$"% E&
Ren"emen a.a
Ke!"#$"% E5
Ren"emen a.a
Ke!"#$"% E'
Ren"emen a.a
5.2. (urn!
5.3. L$"rn Se#entr
=