DAFTAR ISI

HALAMAN JUDUL ................................................................................. i

HALAMAN PENGESAHAN .................................................................... ii
HALAMAN PERNYATAAN ORISINALITAS ........................................ iii
PRA KATA ............................................................................................. iv
HALAMAN PERNYATAAN PERSETUJUAN PUBLIKASI TUGAS
AKHIR UNTUK KEPENTINGAN AKADEMIS ...................................... vi
ABSTRAK ................................................................................................ vii
ABSTACT .................................................................................................. viii
DAFTAR ISI ............................................................................................. ix
DAFTAR GAMBAR ................................................................................. xi
DAFTAR TABEL ..................................................................................... xii
DAFTAR LAMPIRAN .............................................................................. xiii

BAB I PENDAHULUAN
1.1 Latar Belakang ..................................................................................... 1
1.2 Perumusan Masalah ............................................................................ 3
1.3 Tujuan Penelitian ................................................................................. 6
1.4 Manfaat Penelitian ............................................................................... 6
1.5 Ruang Lingkup .................................................................................... 6
1.6 Sistematika Penulisan ........................................................................... 6

BAB II TINJAUAN PUSTAKA

2.1 High Rate Algae Reactor (HRAR) ....................................................... 9
2.1.1 Faktor yang Memengaruhi Kinerja HRAR ................................. 9
2.2 Mikroalga ........................................................................................... 14
2.2.1 Faktor yang Memengaruhi Pertumbuhan Mikroalga ................... 15
2.2.2 Fase Pertumbuhan Mikroalga .................................................... 18
2.2.3 Proses Fotosintesis ..................................................................... 19
2.2.4 Kultivasi Mikroalga ................................................................... 21

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2.3 Bakteri ................................................................................................ 23
2.4 Konsorsium Mikroalga-Bakteri ........................................................... 25
2.5 Palm Oil Mill Effluent (POME) ............................................................ 26

BAB III METODE PENELITIAN

3.1 Alat dan Bahan .................................................................................... 28
3.1.1 Alat ............................................................................................ 28
3.1.2 Bahan ........................................................................................ 28
3.2 Variabel Penelitian .............................................................................. 28
3.2.1 Variabel Bebas .......................................................................... 28
3.2.2 Variabel Tetap ........................................................................... 29
3.2.3 Variabel Terikat ........................................................................ 29
3.3 Prosedur Kerja .................................................................................... 29
3.3.1 Studi Literatur ........................................................................... 30
3.3.2 Persiapan Awal .......................................................................... 30
3.3.3 Penelitian Utama ....................................................................... 32
3.4 Analisis dan Pengolahan Data .............................................................. 32

BAB IV HASIL DAN PEMBAHASAN

4.1 Karakteristik Awal Palm Oil Mill Effluent (POME) ............................ 36
4.2 Identifikasi Mikroalga-Bakteri dalam POME Kolam IV ...................... 36
4.3 Pengaruh Kecepatan Pengadukan terhadap Jumlah Sel Mikroalga dan
Spesific Growth Rate (SGR) ................................................................. 38
4.4 Pengaruh Kecepatan Pengadukan terhadap Nilai Mixed Liquor
Suspended Solid (MLSS) ..................................................................... 41
4.5 Pengaruh Kecepatan Pengadukan terhadap pH Medium ....................... 43
4.6 Pengaruh Kecepatan Pengadukan terhadap Suhu Medium .................... 45
4.7 Pengaruh Kecepatan Pengadukan terhadap Efisiensi Penyisihan COD . 46
4.8 Pengaruh Kecepatan Pengadukan terhadap Efisiensi Penyisihan
Nitrogen Total ..................................................................................... 49

BAB V KESIMPULAN DAN SARAN

5.1 Kesimpulan ......................................................................................... 53

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5.2 Saran ................................................................................................... 53

DAFTAR PUSTAKA ............................................................................... 54

LAMPIRAN ............................................................................................. 59

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 DAFTAR GAMBAR
Gambar 2.1 High Rate Algae Reactor Design ............................................ 8
Gambar 2.2 Ilustrasi Lintasan Mikroalga.................................................... 12
Gambar 2.3 High Rate Algae Reaktor ........................................................ 14
Gambar 2.4 Chlorella sp ............................................................................ 15
Gambar 2.5 Grafik Pertumbuhan Mikroalga ............................................... 18
Gambar 2.6 Reaksi Terang dan Reaksi Gelap ............................................. 20
Gambar 2.7 Ilustrasi Mekanisme Konsorsium Alga-Bakteri ....................... 25
Gambar 3.1 Diagram Alir Prosedur Penelitian............................................ 29
Gambar 3.2 Design HRAR Tampak Atas ................................................... 30
Gambar 3.3 Design HRAR Tampak Samping ............................................ 31
Gambar 3.4 Design HRAR Tampak Depan ................................................ 31
Gambar 3.5 Pola Kotakan Thomasitometer ................................................ 34
Gambar 4.1 Pengamatan Mikroalga Menggunakan Mikroskop................... 37
Gambar 4.2 Grafik Hubungan Kecepatan Pengadukan Terhadap Jumlah
Sel Mikroalga ......................................................................... 39
Gambar 4.3 Grafik Hubungan Kecepatan Pengadukan Terhadap Spesific
Growth Rate Mikroalga .......................................................... 40
Gambar 4.4 Grafik Hubungan Kecepatan Pengadukan Terhadap MLSS ..... 42
Gambar 4.5 Grafik Hubungan Mikroalga dengan MLSS ............................ 43
Gambar 4.6 Grafik Hubungan Kecepatan Pengadukan Terhadap pH .......... 44
Gambar 4.7 Grafik Hubungan Kecepatan Pengadukan Terhadap suhu ....... 45
Gambar 4.8 Grafik Hubungan Kecepatan Pengadukan Terhadap
(a) Penyisihan COD (b) Efisiensi Penyisihan .......................... 47
Gambar 4.9 Grafik Hubungan Penyisihan COD dengan Jumlah Sel
Mikroalga ............................................................................. 48
Gambar 4.10 (a) Nilai Konsentrasi N-total dan (b) Efisiensi Penyisihan
N-total .................................................................................. 50
Gambar 4.11 Grafik Hubungan Penyisihan Nitrogen Total terhadap
Jumlah Sel Mikroalga ........................................................... 51

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 DAFTAR TABEL

Tabel 2.1 Kriteria Desain HRAR................................................................ 9

Tabel 2.2 Perbandingan Open Pond dan Fotobioreaktor Tertutup ............... 21
Tabel 2.3 Fotobioreaktor Sistem Tertutup Berdasarkan Bentuknya ............ 22
Tabel 2.4 Komposisi Bold Bassal Medium ................................................. 23
Tabel 2.5 Larutan Trace Metal BBM ......................................................... 23
Tabel 2.6 Komposisi Jumlah Air Limbah Dari Satu Ton CPO .................... 26
Tabel 3.1 Metode Analisa Penelitian .......................................................... 33
Tabel 4.1 Karakteristik Awal POME Inlet Kolam IV ................................. 36
Tabel 4.2 Identifikasi Mikroalga dari POME .............................................. 37
Tabel 4.3 Perbandingan Penyisihan COD pada Penelitian Lainnya ............. 48
Tabel 4.4 Perbandingan Penyisihan N-total pada Penelitian Lainnya .......... 52

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 DAFTAR LAMPIRAN

Lampiran A Prosedur Analisa COD .......................................................... 59

Lampiran B Prosedur Analisa Nitrogen Total ............................................ 62
Lampiran C Prosedur Analisa Mixed Liquor Suspended Solid (MLSS) ...... 66
Lampiran D Peraturan Menteri Lingkungan Hidup Republik Indonesia
No. 05 Tahun 2014 Tentang Baku Mutu Air Limbah .............. 67
Lampiran E Perhitungan Konversi Kecepatan Paddle ................................ 68
Lampiran F Data Penelitian dan Contoh Perhitungan ................................. 69
Lampiran G Dokumentasi Data Penelitian ................................................. 76

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