DAFTAR ISI

Hal
Lembar Motto dan Persembahan...................................................................... i
Lembar Pengesahan............................................................................................ ii
Halaman Pernyataan Orisinalitas...................................................................... iii
Halaman Persetujuan Publikasi Tugas Akhir Skripsi..................................... iv
Riwayat Hidup..................................................................................................... v
Abstrak................................................................................................................. vi
Abstract................................................................................................................ vii
Kata Pengantar.................................................................................................... viii
Daftar Isi............................................................................................................... x
Daftar Tabel......................................................................................................... xii
Daftar Gambar.................................................................................................... xiii
Daftar Lampiran................................................................................................. xiv
BAB I PENDAHULUAN.................................................................................... 1
1.1 Latar Belakang Masalah.................................................................. 1
1.2 Identifikasi Masalah......................................................................... 4
1.3 Ruang Lingkup................................................................................. 5
1.4 Batasan Masalah............................................................................... 5
1.5 Rumusan Masalah............................................................................ 5
1.6 Tujuan Penelitian............................................................................. 6
1.7 Manfaat Penelitian........................................................................... 6
BAB II TINJAUAN PUSTAKA......................................................................... 7
2.1 Deskripsi Sirih Cina (Peperomia pellucida L.)............................... 7
2.2 Hati..................................................................................................... 12
2.3 Antioksidan....................................................................................... 14
2.4 Bilirubin............................................................................................. 15
2.5 SGOT Dan SGPT.............................................................................. 16
2.6 Parasetamol....................................................................................... 18
2.7 Hepatoprotektif................................................................................. 20
2.8 Tikus Putih (Rattus norvegicus)....................................................... 20
2.9 Kerangka Berfikir............................................................................ 22
BAB III METODE PENELITIAN..................................................................... 23
3.1 Lokasi dan Waktu Penelitian.......................................................... 23
3.2 Populasi dan Sampel........................................................................ 23
3.2.1 Populasi..................................................................................... 23
3.2.2 Sampel....................................................................................... 23
 3.3 Desain dan Variabel Penelitian....................................................... 24
3.3.1 Desain Penelitian...................................................................... 24
3.3.2 Variabel Penelitian.................................................................. 25
3.4 Definisi Operasional......................................................................... 26
3.5 Instrumen Penelitian........................................................................ 27
3.5.1 Alat............................................................................................ 27
3.5.2 Bahan........................................................................................ 27
3.6 Teknik Pengumpulan Data.............................................................. 27
3.6.1 Pengukuran Kadar Bilirubin Total....................................... 27
3.6.2 Pengukuran Kadar Enzin SGOT........................................... 28
3.6.3 Pengukuran Kadar Enzim SGPT.......................................... 28
3.7 Prosedur Penelitian.......................................................................... 28
3.7.1 Pengadaan Tikus Putih (Rattus norvegicus).......................... 28
3.7.2 Aklimatisasi Hewan Uji........................................................... 29
3.7.3 Pembuatan Ekstrak Etanol Daun Sirih Cina (EEDSC)...... 29
3.7.4 Pembuatan suspensi CMC 1%............................................... 32
3.7.5 Pengambilan Darah Hewan Uji.............................................. 32
3.7.6 Penentuan Dosis Parasetamol................................................. 32
3.8 Analisis Data..................................................................................... 33
BAB IV HASIL DAN PEMBAHASAN............................................................. 34
4.1 Hasil Penelitian................................................................................. 34
4.1.1 Bilirubin Total.......................................................................... 34
4.1.2 Serum Glutamic Oxaloacetic Transminase (SGOT).............. 35
4.1.3 Serum Glutamic Pyruvate Transaminase (SGPT).................. 36
4.2 Pembahasan...................................................................................... 38
4.2.1 Bilirubin Total.......................................................................... 38
4.2.2 SGOT dan SGPT..................................................................... 39
BAB V KESIMPULAN DAN SARAN.............................................................. 43
5.1 Kesimpulan........................................................................................ 43
5.2 Saran.................................................................................................. 43
DAFTAR PUSTAKA.......................................................................................... 44
 DAFTAR TABEL
Hal
Tabel 2.1. Tabel Penapisan Skrining Fitokimia..................................................... 9
Tabel 3.1. Desain Penelitian.................................................................................. 25
Tabel 3.2. Prosedur penyiapan sampel Daun Sirih Cina........................................ 30
Tabel 4.1. Pengaruh Pemberian Ekstrak Etanol Daun Sirih Cina (EEDSC) Terhadap
Kadar Bilirubin Total (mg/dl) Pada Tikus Putih Yang Diinduksi Parasertamol
............................................................................................................... 34
Tabel 4.2 Pengaruh Pemberian Ekstrak Etanol Daun Sirih Cina (EEDSC) Terhadap
Kadar Enzim SGOT (U/L) Pada Tikus Putih Yang Diinduksi Parasertamol
............................................................................................................... 36
Tabel 4.3 Pengaruh Pemberian Ekstrak Etanol Daun Sirih Cina (EEDSC) Terhadap
Kadar Enzim SGPT (U/L) Pada Tikus Putih Yang Diinduksi Parasertamol
............................................................................................................... 37
 DAFTAR GAMBAR
Hal
Gambar 2.1. Gambar Tanaman Sirih Cina (Peperomia pellucida L.)................... 7
Gambar 2.2. Gambar Tikus Putih (Rattus norvegicus).......................................... 21
Gambar 2.3. Kerangka Berpikir............................................................................. 22
 DAFTAR LAMPIRAN
Hal
Lampiran 1. Analisis Data Kadar Bilirubin Total................................................. 51
Lampiran 2. Analisis Data Kadar SGOT.............................................................. 52
Lampiran 3. Analisis Data Kadar SGPT............................................................... 53
Lampiran 4. Dokumentasi Penelitian.................................................................... 54
Lampiran 5. Surat Izin Penelitian di Rumah Hewan Laboratorium Kimia FMIPA
UNIMED.......................................................................................... 57
Lampiran 6. Surat Izin Penelitian di Laboratorium Kesehatan Daerah................ 58
Lampiran 7. Surat Selesai Penelitian di Laboratorium Kimia FMIPA UNIMED 59