BIOTEKNOLOGI

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 PENGERTIAN
Bioteknologi adalh cabng ilmu yang
mempelajari pemanfaatan makhluk
hidup (bakteri, virus fungi, dan lain-
lain) maupun produk dari makhluk
hidup (enzim, alkohol) dalam
proses produksi untuk
menghasilkan barang dan jasa.
CIRI UTAMA
BIOTEKNOLOGI
Adanya agen biologi berupa
01 mikroorganisme, tumbuhan atau
hewan

Adanya pedayagunaan secara

02 teknoloogi dan industri..

Produk yang dihasilkan adalah hasil

03 ekstraksi dan pemurnian..
Alasan mikrorganisme dijadikan subjek utama pada
berbagai proses bioteknologi, yaitu :
 Perkembangannya sangat cepat.

 Mudah diperoleh dari lingkungan sekitar.

 Sifatnya tetap.

 Sifat mikroorganisme dapat dimodifiikasi atau

diubah dengan cepat melalui teknik rekyasa
genetika sehinggga dapat menghasilkan produk
yang sesuai dengan yang diinginkan.

 dapat menghasilkan berbagai produk yang

dibutuhkan oel manusia yang tidak bergantung
pada musim atau kondisi iklim lingkungannya
selama mereka dibiiakkan pada sistem tertutup.
JENIS-JENIS
BIOTEKNOLLOGI
BIOTEKNOLOGI KONVENISONAL

Bioteknologii Konvensional adalah

bioteknologi yang menggunakan
mikroorganisme sebagai alat untuk
menghasilkan produk atau jasa,
misalnya jamur dan bakteri yang
menghasilkan enzim-enzim tertentu
untuk melakukan metabolisme
sehingga diperoleh produk yang
diinginkan
JENIS-JENIS
BIOTEKNOLLOGI
BIOTEKNOLOGI MODERN

Bioteknlogi Modern adalah

bioteknologi yang
menggunakan teknik
rekayasa genetika, sepeti
DNA rekombinan
4
 KONVENSIONAL
CONTOH
BIOTEKNOLOGI

TEMPE YOGURT
KEJU

MODERN

KULTUR JARINGAN INSEMINASI BUATAN KLONING

 BIOTEKNOLOGI KONVENSIONAL

Mikroorganisme Mikroorgansime yang Beberapa mikro

yang digunakan digunbakan adalah bak organisme yang
Merupakan hasil fer untuk membuat
mentasi kapang Rhiz teri asam laktat, yaitu : efektif membuat
keju adalah kelo Lactobacillus PST adalah Chl
opus sp. Bahan atau mpok bakteri as
substratnya adalah k bulgaricus dan orella, Spirulina,
am laktat ( Lacto Streptococcus Candida utilis, d
edelai. bacillus lactisda camemberti an Fusarium
n Streptococcus
)
YOGURT
TEMPE PST
KEJU
 TEKNOLOGI REKAYASA
REPRODUKSI GENETiKA
BIOTEKNOLOGI
Suatu teknik
MODERN Cara perbanyakan tumbuhan memanipulasi gen
dan hewan dengan suatu oeganisme
menggunakan prosedur dan untuk memperoleh
peralatan tertentu untuk produk baru,
mendapatkan keturunan Rekayasa genetikaa
secara cepat dan mempunyai dilakukan dengan car
sifat atau karakter yang sama membuat DNA
dengan induknya atau rekombinan melalui
bahkan lebih baik dari penyispan gen
induknya dengann plasmid
sebagai vektornya.
 TEKNOLOGI
REPRODUKSI
 KULTUR JARINGAN

PowerPoint Presentation
Kultur jaringan merupakan teknik
perbanyakan tanaman dengan cara
mengisolasi bagian tanaman seperti daun,
mata tunas, serta menumbuhkan bagian-
bagian tersebut dalam media buatan
secara aseptik yang kaya nutrisi dan zat
pengatur tumbuh dalam wadah tertutup
yang tembus cahaya sehingga bagian
tanaman dapat memperbanyak diri dan
bergenerasi menjadi tanaman lengkap.
Prinsip utama dari teknik kultur jaringan
adalah perbayakan tanaman dengan
menggunakan bagian vegetatif tanaman
menggunakan media buatan yang
dilakukan di tempat steril.
 TEKNOLOGI
REPRODUKSI
 KULTUR JARINGAN

PowerPoint Presentation
Bibit yang dihasilkan dari kultur jaringan
mempunyai beberapa keunggulan, antara
lain: mempunyai sifat yang identik dengan
induknya, dapat diperbanyak dalam
jumlah yang besar sehingga tidak terlalu
membutuhkan tempat yang luas, mampu
menghasilkan bibit dengan jumlah besar
dalam waktu yang singkat, kesehatan dan
mutu bibit lebih terjamin, kecepatan
tumbuh bibit lebih cepat dibandingkan
dengan perbanyakan konvensional.
 TAHAPAN KULTUR JARINGAN

01 02 03 04
Pemilihan dan Inisiasi kultur Sterilisasi Your Text Here

penyiapan tanaman Multiplikasi

induk sumber
eksplan

05 06
Pemanjangan tunas, Your Text Here
Aklimatisasi
induksi, dan
perkembangan
akaar
 JENIS-JENIS KULTUR JARINGAN

1. Kultur Kloroplas
Eksplan yang digunakan adalah kloroplas. Kultur ini
biasanya bertujuan untuk memperbaiki atau
membuuat varietas baru.
2. Kultur Polen
Eksplan yang digunakan adalah serbuk sari.

3. Kultur Protoplas
Eksplan yang digunakan adalah sel jaringan hidup
tapa dinding sel

4. Kultur Meristem
Eksplan yang digunakan aalah jaringan ( akar, batang,
dan daun ) yang muda atau bersifat meristematik.
 MEDIA KULTUR JARINGAN
Media merupakan faktor penentu dalam perbanyakan dengan kultur jaringan.
Komposisi media yang digunakan tergantung dengan jenis tanaman yang
akan diperbanyak. Media yang digunakan biasanya terdiri dari garam
mineral, vitamin, dan hormon. Selain itu, diperlukan juga bahan tambahan
seperti agar, gula, dan lain-lain. Zat pengatur tumbuh (hormon) yang
ditambahkan juga bervariasi, baik jenisnya maupun jumlahnya, tergantung
dengan tujuan dari kultur jaringan yang dilakukan. Media yang sudah jadi
ditempatkan pada tabung reaksi atau botol-botol kaca. Media yang
digunakan juga harus disterilkan dengan cara memanaskannya dengan
autoklaf
 KEUNTUNGAN
PEMANFAATAN KULTUR
JARINGAN Dalam proses pembibitan bebas dari gang
guan hama, penyakit, dan deraan lingkungan
lainnya

Your Text Here

Bibit yang dihasilkan seragam

Your Text Here Your Text Here

Pengadaan bibit tidak tergantung
musim Bibit yang adihasilkan bebas penyakit
(menggunakan organ tertentu)

Your Text Here

Your Text Here Your Text Here
Bibit dapat diproduksi dalam
jumlah banyak dengan waktu yang Biaya pengangkutan bibit relatif
relatif lebih cepat (dari satu mata lebih murah dan mudah
tunas yang sudah respon dalam 1
tahun dapat dihasilkan minimal
10.000 planlet/bibit)
 KEKURANGAN KULTUR JARINGAN

Contents Here

Bibit hasil kultur jaringan membutuhkan

Contents Here proses aklimatisasi karena terbiasa
dalam kondisi steril dan lembap
Biaya yang dibutuhkan untuk
melakukan kultur jaringan relatif besar

Contents Here

Hanya mampu dilakukan oleh orang-

orang tertentu karena membutuhkan
keahlian khusus
 INSEMINASI
BUATAN

Inseiminasi buatan adalah salah satu

teknologi reproduksi yang melalui
perkembangbiakan secara generatif.
teknik ini umumnya dilakukan pada
hewan ternak dan hewan langka.
 INSEMINASI
BUATAN

Fertilisasi ini terjadi di dalam tabung

sehingga lebih dikenal dengan bayi
tabung. Teknik teknologi reproduksi ini
sudah sejak lama dilakukan pada
hewan mamalia, tetapi untuk manusia
baru dilakukan pada tahun 1974.
Proses bayi tabung
Proses bayi tabung terdiri atas pengendalian proses ovulasi
secara hormonal, pemindahan sel telur dari ovarium, dan
pembuahan oleh sel sperma dalam sebuah medium cair.
Secara garis besar prosedur fertilisasi in vitri adalah sebagai
berikut :
 Sel telur diambil dari induk betina dan sperma dari induk
jantan.
 Sel telur dan sperma tersebutr dimasukkan ke dalam tabung
reaksi yang berisi medium cair yang dibutuhkan untuk
terjadinya pembuahan dan kondisinya disesuaikan dengan
kondisi seperti di dalam tubuh.
 Zigot dipelihara pada tabung reaksi hingga berkembang
menjadi embrio.
 Setelah berumur 2-5 hari, embrio terebut dimasukkan atau
ditanam di dinding rahim induk betina untuk proses
pertumbuhan dan perkebangan janin.
 TEKNOLOGI
REPRODUKSI
 KLONING

PowerPoint Presentation
Kloning adalah suatu proses untuk
menghasilkan populasi yang terdiri atas
individu-indiviu yang memiliki sifat genetik
yang sama,. Proses ini di alam umum
ditemukan pada bakteri, serangga, dan
tumbuhan yang memiliki kemampuan
untuk memperbanyak diri secara
aseksual.
REKAYASA
GENETIKA
Beberapa prosedur umum pada pelaksanaan rekayasa gentika,
yaitu :
• Mengidentifikassi gen yang diinginkan.
• Mengisolasi gen donor.'
• Mengekstraksi plasmid dari sel bakteri.
• Membuka plsmid dan menyisipkan potongan DNA
pembawa informasi sesuai dengan yang dikehendaki.
• Memasukkan plasmid berisi DNA rekombinan ke dalam sel
bakteri.
• Membiakkan bakteri yang tekayasa di dalam tabung
fermentasi.
BEBERAPA UNSUR PENTING REKAYASA GENETIKA

i
Pemiindahan sifat-sifat
molekul DNA yang te Untuk memoton
dari suatu mikroba ke
rdapat pada bakteri a g DNA dan men
mikroba lainnya melalu
tau eukariot bersel s gambil gen-gen t
i bagian-bagian DNA te
atu, berbentuk kecil y ertenut. Enzim i
rtentu dkroba satu ke
ang bereplikasi. Yan ni dinamakn enz
mikroba lainnya. Salah
g berfungksi sebagai im restriksi atau
satu contohnya adalah
vektor ( pembawa ) enzim pembatas
pembuatan insulin
gen yang akan disisp .
kan.
TRANSFORMASI
ENZIM
PLASMID
ANTIBIOTIK

Produk metabolisme yang dihasilkan oleh

mikroorganisme tetentu yang mempunyai sifat dapat
menghambat pn atau merusak mikroorganisme lain.
Antibiotik dapat diperoleh dari bakteri atau jamur
yang diproses dengan cara tertentu:
•
 Tahapan-tahapan
pembuatan antibiotik
Mikroorganisme dipindahkan
kedalam bejana fermentasi.

Your Text Here

Mikroorganisme penghasil
antibiotik dikembangbiakkan. Dari cairan biakan itu, antibitik
diekstraksi dan dimurnikan.
Your Text Here
VAKSIN
Untuk melindungi atau mencegah tubuh dari
serangan penyakit. Secara konvensional vaksin
dibuat dari mikroorganisme (bakteri atau virus) yang
dilemahkan atau toksin yang dihasilkan oleh
mikroorganisme tersebut. Akan tetapi, vaksin yang
dihaslikan kurang aman dan dapat menimbulkan
efek yang merugikan.
 EFEK MERUGIKAN

1.
Mikroorgansime yang digunakan untuk
mebuat vaksin kemungkinan masih
melanjutkan proses reproduksi
2.
Mikroorgansime yang digunakan untuk m
kemungkinan masih memiliki kemampuan
menyebabkan penyakit.
3.
Ada orang yang alergi terhadap sisa-sisa sel dari
reproduksi vaksin.
 MEDIA KULTUR JARINGAN

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You can simply impress You can simply impress You can simply impress You can simply impress
your audience and add a your audience and add a your audience and add a your audience and add a
unique zing and appeal to unique zing and appeal to unique zing and appeal to unique zing and appeal to
your Presentations. your Presentations. your Presentations. your Presentations.
 Infographic Style
1. Contents Here A
You can simply impress your audience and add a unique zing and
appeal to your Presentations. Easy to change colors, photos and Text.
Text A

2. Contents Here B
You can simply impress your audience and add a unique zing and
appeal to your Presentations. Easy to change colors, photos and Text.
Text B

3. Contents Here C
You can simply impress your audience and add a unique zing and
appeal to your Presentations. Easy to change colors, photos and Text.
Text C

4. Contents Here D
You can simply impress your audience and add a unique zing and
appeal to your Presentations. Easy to change colors, photos and Text.
Text D
Simple Portfolio
Presentation
Easy to change colors, photos and Text.

Get a modern PowerPoint Presentation that is beautifully designed. Easy to change

colors, photos and Text. You can simply impress your audience and add a unique zing
and appeal to your Presentations. I believe that this Template will your Time, Money and
Reputation. Get a modern PowerPoint Presentation that is beautifully designed. Easy to
change colors, photos and Text. Get a modern PowerPoint Presentation that is
beautifully designed.

You can simply impress your audience and add a unique zing and appeal to your
Presentations. I believe that this Template will your Time, Money and Reputation.

Your Text Here Your Text Here

Get a modern PowerPoint Get a modern PowerPoint
Presentation that is beautifully Presentation that is beautifully
designed. designed.
 Your Text Here Your Text Here
Two Column You can simply impress
your audience and add a
You can simply impress
your audience and add a

Style unique zing and appeal to

your Presentations. Easy
to change colors, photos
and Text. I hope and I
unique zing and appeal to
your Presentations. Easy
to change colors, photos
and Text. I hope and I
believe that this Template believe that this Template
will your Time, Money will your Time, Money
and Reputation. Get a and Reputation. Get a
modern PowerPoint modern PowerPoint
Presentation that is Presentation that is
beautifully designed. beautifully designed.
Easy to change colors, Easy to change colors,
photos and Text. You can photos and Text. You can
simply impress your simply impress your
audience and add a audience and add a
unique zing and appeal to unique zing and appeal to
your Presentations. your Presentations.

You can simply impress your audience

and add a unique zing and appeal to
your Presentations.
One Column Your Text Here
Get a modern PowerPoint Presentation that is beautifully
designed.

Style You can simply impress your audience and add a unique zing and
appeal to your Presentations. I hope and I believe that this
Template will your Time, Money and Reputation. You can simply
impress your audience and add a unique zing and appeal to your
Presentations. You can simply impress your audience and add a
unique zing and appeal to your Presentations. You can simply
impress your audience and add a unique zing and appeal to your
Presentations. I hope and I believe that this Template will your
Time, Money and Reputation.

I hope and I believe that this Template will your Time, Money and
Reputation. You can simply impress your audience and add a
unique zing and appeal to your Presentations.

Get a modern PowerPoint Presentation that is beautifully

designed.

You can simply impress your audience

and add a unique zing and appeal to
your Presentations.
 Thank You
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 Shapes IMAGES

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 Fully Editable Icon Sets: A
You can Resize without
losing quality
You can Change Fill
Color &
Line Color

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 Fully Editable Icon Sets: B
You can Resize without
losing quality
You can Change Fill
Color &
Line Color

FREE
PPT
TEMPLATES
www.allppt.com
 Fully Editable Icon Sets: C
You can Resize without
losing quality
You can Change Fill
Color &
Line Color

FREE
PPT
TEMPLATES
www.allppt.com