DAFTAR ISI

Halaman
DAFTAR ISI...........................................................................................................i
DAFTAR TABEL...................................................................................................ii
DAFTAR GAMBAR..............................................................................................iii
BAB 1 PENDAHULUAN......................................................................................1
Latar Belakang........................................................................................................1
Rumusan Masalah...................................................................................................3
Tujuan Penelitian....................................................................................................3
1.3.1 Tujuan Umum................................................................................................3
1.3.2 Tujuan Khusus...............................................................................................4
1.4 Manfaat Penelitian............................................................................................4
BAB 2 TINJAUAN PUSTAKA.............................................................................5
2.1 Organ Reproduksi Pria......................................................................................5
2.1.1 Spermatogenesis............................................................................................9
2.1.2 Fisiologis Sperma yang Matang.....................................................................12
2.1.3 Semen.............................................................................................................13

2.1.4 Spermatogenesis Abnormal dan Fertilitas pria..............................................14

2.2 Infertilitas..........................................................................................................16
2.3 Teh hijau............................................................................................................18
2.3.1 Jenis Teh.........................................................................................................18
2.3.2 Teh Hijau........................................................................................................18
2.3.3 Taksonomi tumbuhan teh...............................................................................19
2.3.4 Morfologi Tanaman Teh.................................................................................20
2.3.5 Habitat dan Distribusi Geografis...................................................................20
2.3.6 Kandungan Kimia Teh Hijau.........................................................................21
2.3.7 Katekin...........................................................................................................22
3.1 Rokok................................................................................................................23
3.1.1 Definisi Rokok...............................................................................................23
3.1.2 Kandungan Rokok.........................................................................................24
3.1.3 Radikal Bebas dalam Asap Rokok.................................................................24
3.1.4 Peran Radikal Bebas dalam Asap Rokok Terhadap Kerusakan Spermatozoa
4.1 Antioksidan.......................................................................................................25
BAB 3 KERANGKA KONSEP..............................................................................27

3.1 Kerangka Konseptual........................................................................................28

3.2 Hipotesis...........................................................................................................28
BAB 4 METODOLOGI PENELITIAN.................................................................29
4.1 Rancangan Penelitian........................................................................................30
4.2 Lokasi dan Waktu Penelitian.............................................................................30
4.3 Populasi dan Sampel.........................................................................................30
4.3.1 Populasi..........................................................................................................30
4.3.2 Sampel............................................................................................................30
4.3.3 Replikasi........................................................................................................30
4.3.4 Teknik Pengambilan Sampel..........................................................................31
4.3.5 Karakteristik Sampel Penelitian.....................................................................31
4.3.5.1 Kriteria Inklusi............................................................................................31
4.3.5.2 Kriteria Eksklusi.........................................................................................32
4.3.6 Variabel Penelitian.........................................................................................32
4.3.6.1 Variabel Bebas............................................................................................32
4.3.6.2 Variabel Tergantung....................................................................................32
4.3.7 Definisi Operasional Variabel........................................................................32

4.4 Bahan dan Instrumen Penelitian.......................................................................33

4.4.1 Pemeliharaan Tikus..................................................................................33
4.4.2 Pemaparan Asap Rokok pada strain wistar..............................................33
4.4.3 Pembuatan Ekstrak Teh Hijau..................................................................34
4.5 Prosedur Penelitian...........................................................................................34
4.5.1 Pembagian Kelompok Tikus..........................................................................34
4.5.2 Aklimatisasi....................................................................................................35
4.5.3 Ekstrak Teh Hijau...........................................................................................35
4.5.4 Pemaparan Asap Rokok.................................................................................37
4.5.5 Pengambilan Spermatozoa.............................................................................37
4.5.6 Perhitungan Spermatozoa..............................................................................38
4.6 Alur Penelitian..................................................................................................39
4.7 Analisis Data.....................................................................................................40
DAFTAR PUSTAKA..............................................................................................41

DAFTAR TABEL
Tabel 2.1 Komposisi Kandungan Zat Kimia Dalam Teh........................................22

DAFTAR GAMBAR
Gambar 2.1 Struktur Testis.....................................................................................5
Gambar 2.2 Tubulus Seminiferus...........................................................................6
Gambar 2.3 Organ Reproduksi Pria........................................................................7
Gambar 2.4 Proses Spermatogenesis......................................................................11
Gambar 2.5 Struktur Sperma..................................................................................12
Gambar 2.6 Sperma Infertilitas Abnormal..............................................................15
Gambar 2.7 Tanaman Teh Hijau.............................................................................20
Gambar 2.8 Struktur Kimia EGCG.........................................................................23
Gambar 2.9 Kerangka Konsep................................................................................28