PRAKTIKUM MIKROBIOLOGI

ANALISA KUANTITATIF MIKROORGANISME

(HITUNGAN CAWAN)

a. Waktu Pelaksanaan
Hari / Tanggal :
b. Tujuan Praktikum
Mahasiswa melakukan penghitungan jumlah mikroba dengan metode hitungan cawan.
c. Dasar Teori
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d. Alat dan Bahan
1. Sampel cair 4. Pipet 1 ml yang steril
2. Alkohol 95% dalam gelas piala 5. Nutrient agar
3. Tabung reaksi berisi NaCl fisiologis 6. Petri dish
steril 7. Alat penghitung koloni
e. Cara Kerja
1. Siapkan pengenceran serial seperti pada praktikum MPN
2. Secara aseptis, lakukanlah pemindahan sampel dari tabung pengencer ke dalam cawan petri
yang telah berisi nutrient agar.

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 3. Homogenkan, beri label dan bungkus cawan tersebut dengan posisi tutup cawan petri berada
di bawah.
4. Inkubasikan pada suhu 370C selama 48 jam.
f. Hasil Pengamatan
Tabel Pengamatan :

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 g. Pembahasan
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h. Kesimpulan
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i. Daftar Pustaka
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NILAI Yogyakarta,.........................................
Dosen Pembimbing Praktikan

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