ABSTRAK

Pengaruh Waktu Inkubasi Antibodi Primer Helicobacter pylori

Terhadap Ketajaman Warna Reaksi Pada Pemeriksaan Autostaining
Imunohistokimia Biopsi Gaster Pasien Gastritis

Peneliti : Nani Jaini

Pembimbing : Neni Oktiyani, Ahmad Muhlisin
Inkubasi antibodi primer merupakan protokol yang harus dikerjakan pada
proses Imunohistokimia inkubasi yang mengalami masalah menyebabkan
buruknya intensitas pewarnaan. Protokol pengecatan Imunohistokimia pada biopsi
gaster pasien gastritis menggunakan alat Autostaining Roche Tissue Diagnostics
Ventana BenchMark XT tidak menyertakan waktu inkubasi dari antibodi primer
Helicobacter pylori. Uji pendahuluan dengan waktu inkubasi 16 menit didapatkan
warna reaksi ideal dan waktu inkubasi 20 menit warna reaksi overstaining,
sedangkan waktu inkubasi di bawah 16 menit belum diketahui warna reaksinya.
Tujuan penelitian ini adalah mengetahui ketajaman warna reaksi pemeriksaan
Autostaining Imunohistokimia biopsi gaster pasien gastritis Helicobacter pylori
dengan waktu inkubasi antibodi primer 8 menit, 12 menit dan 16 menit. Penelitian
dilakukan di Instalasi Laboratorium Patologi Anatomi RSUD Ulin Banjarmasin
pada Juli 2022. Penelitian quasi eksperiment dengan rancangan Postest Only
Control Group Design. Melalui pemeriksaan ketajaman warna reaksi dengan
waktu inkubasi antibodi primer 8 menit, 12 menit dan 16 menit. Hasil
pemeriksaan dianalisis statistik dengan uji Friedman untuk melihat ada atau
tidaknya perbedaan pengaruh antar perlakuan, sedangkan untuk mengetahui waktu
mana yang memberikan pengaruh dilakukan uji Wilcoxon dengan tingkat
kepercayaan 95%. Hasil uji menunjukkan nilai signifikansi 0,000 (< 0,05) artinya
adanya perbedaan bermakna antara waktu inkubasi antibodi primer Helicobacter
pylori terhadap ketajaman warna reaksi. Waktu inkubasi yang berpengaruh
terhadap ketajaman warna reaksi adalah 16 menit. Rerata nilai skor ketajaman
warna reaksi tertinggi adalah 2,89 untuk waktu inkubasi 16 menit. Sehingga
disarankan untuk menggunakan waktu inkubasi antibodi primer Helicobter pylori
selama 16 menit pada pengecatan Autostaining Imunohistokimia.

Kata Kunci : Waktu inkubasi, antibodi primer, ketajaman warna

viii
 ABSTRACT

Effect of Helicobacter pylori Primary Antibody Incubation Time

Against The Sharpness of Color Reaction in Autostaining
Immunohistochemistry Examination of Gastric Biopsy in Gastritis Patients

Researcher : Nani Jaini

Advisors : Neni Oktiyani, Ahmad Muhlisin

Primary antibody incubation is a protocol that must be done in the

incubation Immunohistochemistry process which has problems causing poor
staining intensity. Immunohistochemical staining protocol on gastric biopsies of
gastritis patients using the Autostaining Roche Tissue Diagnostics Ventana
BenchMark XT does not include the incubation time of the primary antibody
Helicobacter pylori. Preliminary test with an incubation time of 16 minutes
obtained the ideal reaction color and an incubation time of 20 minutes for the
color of the overstaining reaction, while the incubation time was below 16
minutes and the reaction color was unknown. The purpose of this study was to
determine the color acuity of the autostaining immunohistochemical examination
of gastric biopsies in Helicobacter pylori gastritis patients with primary antibody
incubation times of 8 minutes, 12 minutes and 16 minutes. The research was
conducted at the Anatomical Pathology Laboratory Installation of Ulin Hospital,
Banjarmasin in July 2022. This was a quasi-experimental study using the Posttest
Only Control Group Design. By examining the color acuity of the reaction with
primary antibody incubation times of 8 minutes, 12 minutes and 16 minutes. The
results of the examination were analyzed statistically with the Friedman test to see
whether or not there was a difference in effect between treatments, while to find
out which time had an effect, the Wilcoxon test was carried out with a 95%
confidence level. The test results showed a significance value of 0.000 (<0.05)
meaning that there was a significant difference between the incubation time of the
Helicobacter pylori primary antibody and the color acuity of the reaction. The
incubation time that affects the color sharpness of the reaction is 16 minutes. The
highest average reaction color sharpness score was 2.89 for 16 minutes of
incubation time. So it is advisable to use Helicobter pylori primary antibody
incubation time of 16 minutes in Autostaining Immunohistochemistry.

Keywords : Incubation time, primary antibody, color acuity

ix