PERHITUNGAN KETIDAKPASTIAN PENGUJIAN MIKROBIOLOGI

Replikasi Pengujian Bakteri E.Coli

AMDK
12 Pebruari 2021
2
No Simplo Duplo Log10 S Log10 D Log10 S - Log10 D Log10 S + Log10 D RSD R
MPN/100ml MPN/100ml
1 48000 60000 4.6812 4.7782 0.0969 9.4594 0.00021
2 36000 28000 4.5563 4.4472 0.1091 9.0035 0.00029
3 44000 32000 4.6435 4.5051 0.1383 9.1486 0.00046
4 36000 48000 4.5563 4.6812 0.1249 9.2375 0.00037
5 40000 52000 4.6021 4.7160 0.1139 9.3181 0.00030
6 64000 56000 4.8062 4.7482 0.0580 9.5544 0.00007
7 60000 48000 4.7782 4.6812 0.0969 9.4594 0.00021
8 32000 36000 4.5051 4.5563 0.0512 9.0615 0.00006
9 36000 28000 4.5563 4.4472 0.1091 9.0035 0.00029
10 36000 48000 4.5563 4.6812 0.1249 9.2375 0.00037
11 28000 32000 4.4472 4.5051 0.0580 8.9523 0.00008
12 52000 44000 4.7160 4.6435 0.0726 9.3595 0.00012
13 48000 60000 4.6812 4.7782 0.0969 9.4594 0.00021
14 76000 68000 4.8808 4.8325 0.0483 9.7133 0.00005
15 48000 48000 4.6812 4.6812 0.0000 9.3625 0.00000
0.0001471
RSD RC 0.0121267

misalnya hasil pengujian sampel Total Coli = 12 MPN/100ml

maka:
Log10 (12) = 1.0792 + 2(0.0121267)

= 1.0792 + 0.024253

= 1.0549 - 1.1035

anti log 11 - 13
jadi pelaporan hasil memiliki rentang
Laporan ( 11 - 13 ) MPN/100ml
atau
Laporan ( 12 +1 ) MPN/100ml

SPV Lab.Mikrobiologi & Fisika Analis

(Dra.Sri Rachmawati) (.Lubis.) ( Retno.)