Nama : Dyan Pitaloka

NIM : 1906110291

Kelas : Agroteknologi A

Tugas 1 Kultur Jaringan dan Manipulasi Genetik Tanaman In-Vitro

Carilah komponen-komponen dalam media (dibuat per liter)!

1. Hara Makro

Jenis Media
Komponen
Murashige Vacin &
Media Gamborg White Nitsch &
& Skoog Went
(mg/liter) (B5) (W) Nitsch
(MS) (VW)
KNO3 1900 2500 80 525 950
NaH2PO4.H2O 150 16.5
KH2PO4 170 250 68
K2SO4
Ca3(PO4)2 200
MgSO4.7H2O 370 250 720 250 185
NH4NO3 1650 720
Na2SO4 200
CaCl2.2H2O 440 150 166
KCl 65
CaNO3.4H2O 300
(NH4)2SO4 134 500

2. Hara Mikro

Jenis Media
Komponen Murashige Vacin &
Gamborg White Nitsch &
Media (mg/liter) & Skoog Went
(B5) (W) Nitsch
(MS) (VW)
Co(NO3)2.6H2O
ZnSO4.7H2O 8.6 2 2.6 10
MnCl2
CuSO4.5H2O 0.025 0.025 0.025
Kl 0.83 0.75 0.75
Fe(C4H4O6)3.2H2O 28
MnSO4.4H2O 22.30 7 0.75 25
Na2EDTA 37.3 37.3 37.3
H3BO3 6.20 3 1.5 10
CoCl2.6H2O 0.025 0.025
MnSO4.H2O
 FeSO4.7H2O 27.8 27.8 27.8
Na2MoO4.2H2O 0.25 0.25 0.25

3. Vitamin dan Sumplemen lainnya

Jenis Media
Komponen
Murashige Vacin &
Media Gamborg White Nitsch &
& Skoog Went
(mg/liter) (B5) (W) Nitsch
(MS) (VW)
Ca- 1
panthothenate
Inositol 100 100 100
Folic acid 0.5
Nicotinic 0.5 0.5 5
acid
Riboflavin
Thiamine 0.1 10 0.1 0.5
HCl
Biotin 0.05
Pyridoxine 0.5 0.1 0.5
HCl
Glycine 2 2 3 2
Cysteine HCl 1