1ppm = 1ug/1ml
1000 ppm
= 1000 ug/1ml
= 1000000 ug/ 1000 ml
= 1000 mg/1000 ml
= 1g/1L
M1V1 = M2V2
1000(V1) = 100(50)
V1 = 5ml
c) Membuat pengenceran 100 ppm ke 2 ppm, 4 ppm, 5 ppm, 6 ppm dan 8 ppm.
2ppm
M1V1 = M2V2
100(V1) = 2(10)
V1 = 0.2ml
4ppm
M1V1 = M2V2
100(V1) = 4(10)
V1 = 0.4ml
5ppm
M1V1 = M2V2
100(V1) = 5(10)
V1 = 0.5ml
6ppm
M1V1 = M2V2
100(V1) = 6(10)
V1 = 0.6ml
8ppm
M1V1 = M2V2
100(V1) = 8(10)
V1 = 0.8ml
Dicari absorbance bagi tiap konsentrasi(ppm), dibuat kurva absorbance spekto uv vis
terhadap konsentrasi panadol baku, lalu mendapatkan persamaan :
y = 0.085x + 0.06
Pengukuran absorbansi tablet panadol pada pH 1.2, 4.6 dan 6.8 dengan interval masa
10,15,20,25 dan 30 menit
Buffer pH 1.2
Menit
10
15
20
25
30
Absorbansi 1
0.277
0.5118
0.4875
0.51
0.4592
Absorbansi 2
0.2764
0.5122
0.4875
0.5076
0.4593
Absorbansi 3
0.2763
0.5123
0.4877
0.5062
0.4595
Rata-rata
0.2766
0.5121
0.4876
0.5079
0.4593
Buffer pH 4.6
Menit
10
15
20
25
30
Absorbansi 1
0.3893
0.4657
0.4729
0.4498
0.4779
Absorbansi 2
0.3897
0.4653
0.4723
0.4497
0.4768
Absorbansi 3
0.3891
0.4654
0.4728
0.45
0.4761
Rata-rata
0.3894
0.4655
0.4727
0.4498
0.4769
Absorbansi 3
0.3484
0.4398
0.5005
0.5181
0.4929
Rata-rata
0.3479
0.4397
0.5
0.5187
0.4931
Buffer pH 6.8
Menit
10
15
20
25
30
Absorbansi 1
0.3475
0.4392
0.4994
0.5189
0.4934
Absorbansi 2
0.3479
0.44
0.5001
0.5184
0.4931
pada 10menit
[A] = 0.3250
y = 0.085x + 0.06
0.3250 = 0.085x + 0.06
x = 3.117 ppm
3.12 ppm = x ug/ 900 ml
x ug = 2808 ug
= 2.808 mg
pada 15menit
[A] = 0.5149
y = 0.085x + 0.06
0.5149 = 0.085x + 0.06
x = 5.35ppm
5.35 ppm = x ug/ 895 ml
x ug = 4788.25 ug
= 4.788 mg
pada 20menit
[A] = 0.6558
y = 0.085x + 0.06
0.6558 = 0.085x + 0.06
x = 7.01 ppm
7.01 ppm = x ug/ 890 ml
x ug = 62 38.9 ug
= 6.239 mg
pada 25menit
[A] = 0.5976
y = 0.085x + 0.06
0.5976 = 0.085x + 0.06
x = 6.32ppm
6.32ppm = x ug/885
= 5593.2ug
= 5.59 mg
pada 30menit
[A] = 0.5519
y = 0.085x + 0.06
0.5519 = 0.085x + 0.06
x = 5.78 ppm
5.78 ppm = x ug/ 880 ml
x ug = 5086.4 ug
= 5.086 mg
Buffer pH 1.2
pada menit ke 10
[A] = 0.2766
y = 0.085x + 0.06
0.2766 = 0.085x + 0.06
X = 2.55 ppm
2.55 ppm = x ug/ 900 ml
x ug = 2295 ug
= 2.295 mg
pada menit ke 15
[A] = 0.5121
Y = 0.085x + 0.06
0.5121 = 0.085x + 0.06
x = 5.32 ppm
5.32 ppm = x ug/ 900 ml
x ug = 4788 ug
= 4.788 mg
pada menit ke 20
[A] = 0.4876
y = 0.085x + 0.06
0.4876 = 0.085x + 0.06
x = 5.031 ppm
5.031 ppm = x ug/ 900 ml
x ug = 4528 ug
= 4.528 mg
pada menit ke 25
[A] = 0.5079
y = 0.085x + 0.06
0.5079 = 0.085x + 0.06
x = 5.27 ppm
5.27 ppm = x ug/ 900 ml
x ug = 4743 ug = 4.743mg
pada menit ke 30
[A] = 0.4593
y = 0.085x + 0.06
0.4593 = 0.085x + 0.06
x = 4.7 ppm
4.7 ppm
= x ug/ 900 ml
x ug = 4230 ug
= 4.23 mg
Buffer pH 4.6
pada menit ke 10
[A] = 0.3894
y = 0.085x + 0.06
0.3894 = 0.085x + 0.06
x = 3.88 ppm
3.88 ppm = x ug/ 900 ml
x ug = 3492 ug
= 3.492 mg
pada menit ke 15
[A] = 0.4655
y = 0.085x + 0.06
0.4655 = 0.085x + 0.06
x = 4.77 ppm
47.7 ppm = x ug/ 900 ml
x ug = 4293 ug
= 4.293 mg
pada menit ke 20
[A] = 0.4727
y = 0.085x + 0.06
0.4727 = 0.085x + 0.06
x = 4.86 ppm
4.86 ppm = x ug/ 900 ml
x ug = 4374 ug
= 4.374 mg
pada menit ke 25
[A] = 0.4498
y = 0.085x + 0.06
0.5121 = 0.085x + 0.06
x = 5.32 ppm
5.32 ppm = x ug/ 900 ml
x ug = 4788 ug
= 4.788 mg
pada menit ke 30
[A] = 0.4769
y = 0.085x + 0.06
0.4769 = 0.085x + 0.06
x = 4.91 ppm
4.91ppm = x ug/ 900 ml
x ug = 4419 ug
= 4.419 mg
Buffer pH 6.8
pada menit ke 10
[A] = 0.3479
y = 0.085x + 0.06
0.3479 = 0.085x + 0.06
x = 3.39 ppm
3.39 ppm = x ug/ 900 ml
x ug = 3051 ug
= 3.051mg
pada menit ke 15
[A] = 0.4397
y = 0.085x + 0.06
0.4397 = 0.085x + 0.06
x = 4.47 ppm
pada menit ke 20
[A] = 0.5
y = 0.085x + 0.06
0.5
= 0.085x + 0.06
x = 5.176 ppm
pada menit ke 25
[A] = 0.5187
y = 0.085x + 0.06
0.5187 = 0.085x + 0.06
x = 5.396 ppm
5.396 ppm = x ug/ 900 ml
x ug = 4856 ug
= 4.856 mg
pada menit ke 30
[A] = 0.4931
y = 0.085x + 0.06
0.4931 = 0.085x + 0.06
x = 5.1 ppm
5.1 ppm = x ug/ 900 ml
x ug = 4590 ug
= 4.590 mg
0.8
0.7
0.6
0.5
0.4
Absorbance
0.3
0.2
0.1
0
2
Konsentrasi (ppm)
Konsentrasi (mg)
6
5
4
3
Reference Drug
Test Drug
1
0
0
10
15
20
Waktu (menit)
25
30
35
Konsentrasi (mg)
6
5
4
3
Reference Drug
Test Drug
1
0
0
10
15
20
25
30
35
Waktu (menit)
Konsentrasi (mg)
6
5
4
3
Reference Drug
Test Drug
1
0
0
10
15
20
Waktu (menit)
25
30
35