Variabel dalam
Penelitian
Miftahul Mushlih, M.Sc.
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Jenis Variabel
V. Suatu Variabel yang di
Bebas control oleh peneliti
Variabel yang
Hasilnya berasal dari V.
V. Bebas Terikat
Contoh
Seorang peneliti ingin mengetahui pegaruh pupuk terhadap beberapa
jenis varieties padi yaitu varieties raja lele dan varieties tawon,
kosentrasi yang digunakan adalah 10 ppm, 20 ppm, 30 ppm, 40 ppm,
dari penelitian tersebut tentukan
A. jenis data (para metric/ non parametric)
B. V. Bebas
C. V. Kontrol
D. V. terikat
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Sample Size
Miftahul Mushlih, M.Sc
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Contoh
Seorang peneliti ingin mengetahui perbedaan penghasilan masyarakat
lubuk buaya dengan kota tengah . . ..
Sd
X1-x2
Paired Studies
Paired studies compare values before and after an intervention in the
same animal. In this case, data are analyzed by a paired t test, and the
sample size is computed by
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Federer Formula
If the s, d and C cannot be determined Federers
Formula
(t-1) (r-1) 15
t = number of treatment
r = number of sample (repeat)
(t-1) (r-1) 15
(3-1)(r-1) 15
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contoh
Penelitian
Pengaruh pemberian ekstrak tapak dara terhadap pertumbuhan
sel-sel kanker payudara
Perlakuan
1. 0 mg/kg bb mencit
2. 10 mg/ kg bb mencit
3. 30 mg/ kg bb mencit
4. 50 mg/ kg bb mencit
Berapa ulangan minimal ulangan pada penelitian tersebut?
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Perumusan berdasarkan
proporsi
Keterangan:
n = Jumlah sampel minimal
N = ukuran populasi
t = tingkat kepercayaan (digunakan 0,95 sehingga nilai t
= 1,96)
d = taraf kekeliruan (digunakan 0,05)
p = proporsi dari karakteristik tertentu (golongan)
q= 1 p
1 = Bilangan Konstan
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Contoh
Berdasarkan proporsi
Secara kuantitaf besarnya sampel dapat ditentukan dengan
menggunakan rumus
matematika sebagai berikut:
1. Meneliti harga mean:
Keterangan:
d : Penyimpangan yang ditoleransi
: harga standar normal
a : varian populasi.
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Preclinical Animal
Study
Preclinical study/preclinical
trial
Preclinical trial - a laboratory test of a new drug or a new medical
device, usually done on animal subjects, to see if the hoped-for
treatment really works and if it is safe to test on humans.
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Experimental study
In vitro and in vivo
Animal models
Pharmacodynamic study
Toxicological study
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PHARMACODYNAMIC STUDIES
Effects / Efficacy
Mechanism of action
TOXICOLOGICAL STUDIES
Safety
Spectrum
Pharmacodynamic study
In vitro : tissue, cell culture, blood
. component, etc
In vivo : whole animal
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Methodology of
pharmacodynamic study
ANIMALS :
Rodent or non-rodent (may depend on desired effect)
Healthy or diseased-animal model
Sex : male and/or female
Number : adequate for statistical analysis
Toxicological studies
IN VIVO TOXICOLOGICAL STUDIES
A. GENERAL TESTS
SHORT TERM
Acute Toxicity Test (LD50)
LONG TERM:
Sub Acute Test
Sub Chronic Test
Chronic Test
B. LOCAL TOXICITY TEST
Dermatological Preparation
C. SPECIAL TOXICITY TESTS
Mutagenicity Test
Carcinogenicity Test
Reproductive and Development Toxicity Test
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Animal models
Animal models are, at the best, analogous
to human condition but no theory can be
provided or refused by analogy. The truth is
the evidence in animals can be a powerful
device in support of virtually theory.
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R eduction
correct model
genetic homogenity
use healthy animal
R efinement
caring
treatment
non-invasive methode
pain, stress, distress minimization
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Human interactions
Enrichment
Devices for NHP
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