LEMBAR KEGIATAN SISWA (WORKSHEET)

KD 3.2

ENZIM KATALASE

A. Tujuan :
1. Mengetahui peranan enzim katalase.
2. Mengetahui faktor-faktor yang mempengaruhi kerja enzim katalase.

B. Alat dan Bahan :

Alat : 1. Tabung reaksi 10 buah
2. Rak tabung reaksi 1 buah
3. Pembakar spiritus 1 buah
4. Pipet tetes 2 buah
5. Gelas ukur 2 buah
6. Lidi dan korek api

Bahan : 1. Ekstrak hati ayam

2. Ekstrak jantung ayam
3. Peroksida air (H2O2) 10%
4. HCl 10%
5. NaOH/KOH 10%

C. Cara Kerja :

Lakukan kegiatan seperti gambar dibawah ini !

Keterangan :
1. Teteskan tabung A ke tabung1, tabung B ke tabung 2, tabung C ke tabung 3, tabung D ke
tabung 4, tabung E ke tabung 5.
2. Pada waktu mereaksikan, tutuplah tabung reaksi dengan ibu jari dan amati pembentukan
gelembung gas kemudian masukkan bara api pada tabung tersebut. Amati keadaan bara api
yang dimasukkan tadi, masukkan ke dalam tabel pengamatan berikut.

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D. Data Hasil Pengamatan
No Perlakuan Gelembung gas *) Nyala api
1. Ekstrak hati + H2O2
2. Ekstrak hati + HCl + H2O2
3. Ekstrak hati + KOH + H2O2
4. Ekstrak hati panas (>38oC)+ H2O2
5. Ekstrak hati + es ( < 20oC) + H2O2
6. Ekstrak jantung + H2O2

*) bubuhkan tanda :
- bila tak ada gelembung
+ bila sedikit
++ bila sedang
+++ bila banyak
++++ bila banyak sekali

E. Bahan Diskusi
1. Pada perlakuan manakah pembentukan gelembung gas paling banyak? Apa sebabnya?
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2. Gas apakah yang terbentuk pada reaksi tersebut?
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3. Apa fungsi lidi membara dalam percobaan tersebut?
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4. Mengapa H2O2 setelah dicampur dengan ekstrak harus ditutupi dengan ibu jari?
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5. Faktor apa saja yang nampak pada percobaan tersebut yang mempengaruhi kerja enzim katalase?
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6. Selain faktor yang nampak pada percobaan tersebut, sebutkan faktor-faktor lain yang
mempengaruhi kerja enzim katalase!
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7. a. Di dalam sel yang hidup dihasilkan H2O2, dari peristiwa apakah sel tersebut
dihasilkan? ......................................................................................................................................
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b. Apa yang terjadi dalam tubuh jika banyak tertimbun H2O2 ?
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c. Bagaimana usaha tubuh untuk menetralisirnya? Tulislah reaksi kimianya!
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8. Berikan contoh enzim lain yang terlibat dalam proses metabolisme, serta jelaskan peranan
masing-masing!
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9. Perhatikan diagram kerja enzim berikut ini!

Berdasarkan gambar di atas, ada dua hipotesis mengenai kerja enzim. Jelaskan perbedaan kedua
hipotesis
tersebut! .............................................................................................................................................
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10. Apa kesimpulan dari kegiatan percobaan tersebut?

Susunlah laporan tertulis dari hasil kegiatan ini.

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