BIOKIMIA VETERINER

REVIEW JURNAL

“Plasma lipids in normal cows around partus and in cows with metabolic
disorders with and without fatty liver”

ANDI ADIYAKSA BAHAR

C031231046

KELAS A

PROGRAM STUDI KEDOKTERAN HEWAN

FAKULTAS KEDOKTERAN

UNIVERSITAS HASANUDDIN

2023
1. Judul Penelitian : Plasma lipids in normal cows around partus and in
cows with metabolic disorders with and without
fatty liver
2. Jurnal : Acta vet. Scand
3. Volume dan Tahun : Vol. 30, Hal. 441-445 / 1989
Judul Penelitian Plasma lipids in normal cows around partus and in
cows with metabolic disorders with and without
fatty liver
Nama Jurnal Acta vet. Scand
Halaman, Vol 441-445, 30
Tahun 1989
Penulis Paul Holtenius
Reviewer Andi Adiyaksa Bahar
Tanggal 8 September 2023

Latar Belakang Penelitian ini dilakukan karena adanya variasi

Masalah metobolisme lemak yang berbeda antara sapi normal
saat partus dan sapi yang mengalami gangguan
metabolisme.
Tujuan Penelitian Penelitian ini bertujuan untuk menjelaskan
pathogenesis perlemakan liver pada sapi dengan
mempelajari pengaruh pemberian pakan, kehamilan,
dan awal laktasi terhadap lipid plasma pada sapi
normal, serta menyelidiki lipid plasma pada sapi
yang mengalami perlemakan hati dan pada sapi
yang mengalami ketosis tanpa atau dengan sedikit
infiltrasi lemak di dalam liver.
Subjek Penelitian 16 sapi Swedia multipara normal dengan produksi
susu rata-rata 7900 kg FCM/tahun, 12 ekor sapi
Swedia primipara normal, 20 ekor sapi dengan
pergeseran abomasum kiri dan perlemakan hati, 16
ekor sapi dengan sedikit infiltrasi lemak di hati.
Metode Penelitian Penelitian dilakukan dengan metode pengamatan
subjek penelitian yang diteliti, dimana dalam hal ini
dilakukan pengamatan asam lemak, kolestrol, dan
fosfolipid dalam plasma lipid saat 6 minngu
sebelum melahirkan dan 6 minggu setelah
melahirkan.
Metode Pengambilan Data diambil dengan menggunakan metode
Data observasi jangka panjang
Pelaksanaan Penelitian Klinik Hewan dan Peternakan Department of Cattle
and Sheep Diseases, Swedish University of
Agricultural Sciences, Uppsala.
 TUGAS BIOKIMIA VETERINER
REVIEW JURNAL
ANDI ADIYAKSA BAHAR
C031231046
KEDOKTERAN HEWAN UNIVERSITAS HASANUDDIN

Plasma Lipids in Normal Cows around Partus and in Cows

Judul Jurnal
with Metabolic Disorders with and without Fatty Liver
Jurnal Acta vet. scand
Tahun 1989
Penulis Paul Holtenius

1. PENDAHULUAN
Pada hewan ruminansia, tidak ada lipid plasma yang berasal dari lemak
hasil pencernaan. Sebagian besar merupakan hasil sintesis nukeleotida de
novo. Lemak disimpan sebagai trigliserida dan kemudian diangkut sebagai
asam lemak bebas yang terikat pada albumen. Sebagian besar dari asam-asam
ini diambil oleh hati. Di sana mereka dapat dioksidasi menjadi CO2 atau
menjadi keton atau diesterifikasi dan digabungkan dengan fosfolipid, kolestrol,
dab apoprotein untuk membentuk lipoprotein terutama lipoprotein dengan
densitas yang sangat rendah. Fraksi lipoprotein ini mengangkut trigliserida ke
berbagai organ organ dan jaringan. Setelah hilangnya trigliserida, lipoprotein
densitas rendah akan dirombak dan setelah metabolisme lebih lanjut menjadi
lipoprotein densitas tinggi.

2. TUJUAN
Untuk menjelaskan pathogenesis perlemakan liver pada sapi dengan
mempelajari pengaruh pemberian pakan, kehamilan, dan awal laktasi
terhadap lipid plasma pada sapi normal, serta menyelidiki lipid plasma pada
sapi yang mengalami perlemakan hati danpada sapi yang mengalami ketosis
tanpa atau dengan sedikit infiltrasi lemak di dalam liver.

3. METODE PENELITIAN
Pada penelitian ini dilakukan metode penelitian kuantitatif eksperimen,
dimana sapi diteliti 6 minggu sebelum melahirkan dan 6 minggu setelah
melahirkan.
 4. ISI/PEMBAHASAN
Hasil dari studi lipid plasma pada 16
ekor sapi nomal disekitar masa melahirkan
ditunjukkan pada figure 1. Dari 6 minggu
hingga 2 minggu sebelum melahirkan,
terdapat sedikit peningkatan FFA dari 0,13-
0,05 menjadi 0,17-0,04 mmol/L. Kemudian
terjadi peningkatan tajam hingga sehari
setelah melahirkan ketika levelnya menjadi
0,54-0,44 mmol/L. Dua minggu setelahnya, levelnya turun menjadi 0,36-0,17
mmol/L dan setelah 4 minggu menjadi 0,30-0,17 mmol/L dan setelah 6
minggu menjadi 0,26-0,11 mmol/L.
Nilai kolestrol plasma turun secara bertahap dari 6 minggu sebelum
hingga sehari setelah melahirkan dari 3,69-0,87 mmol/L menjadi 2,20-0,42
mmol/L. Selama 6 minggu berikutnya, levelnya terus meningkat menjadi
4,98-1,11 mmol/L. Nilai fosfolipid juga turun dari 6 minggu sebelumnya ke
tingkat minimum pada hari setelah melahirkan. Nilainya adalah 1,83-0,49 dan
1,24-0,24 mmol/L berulang secara cepat. Kemudian jumlahnya meningkat
dan mencapai 2,67-0,50 mmol/L, 6 minggu
setelah kalibrasi.
Terdapat peningkatan yang kuat
pada rasio FFA/kolestrol dari 2 minggu
sebelum hingga sehari setelah melahirkan
dan penurunan setelahnya (figure 2).
Perubahan parameter dari 6 minggu
setelahnya semuanya signifikan (p<0,01).
Hasil dari studi lipid plasma pada sapi yang baru pertama kali melahirkan
disajikan pada table 1. Tingkat asam lemak bebas sehari setelah melahirkan
lebih rendah pada hewan-hewan ini dibandingkan dengan sapi-sapi yang lebih
tua. Intensitas pemberian pakan yang tinggi tidak berpengaruh pada lipd
plasma. Mereka sangat mirip pada kedua kelompok. Tingkat lipd plasma pada
sapi dengan perlemakan hati sangat berbeda dengan jumlah pada hewan
normal pada waktu yang sesuai sejak melahirkan (table 2). Sapi-sapi dengan
perlemakan hati memiliki kadar FFA yang lebih tinggi, jumlah kolestrol dan
 fosfolipid yang lebih rendah.

5. KELEBIHAN
 Terdapat kesesuaian antara tujuan jurnal dan kesimpulan yang
didapatkan
 Penyajian gambar dan tabel yang kemudian dijelaskan lagi sehingga
mudah dipahami

6. KEKURANGAN
 Ada beberapa singkatan yang tidak memiliki penjelasan sehingga
pembaca kesulitan mengartikan singkatan tersebut

7. KESIMPULAN
Penelitian ini menunjukkan bahwa sapi-sapi dengan perlemakan hati
memiliki kadar FFA plasma yang sangat tinggi dan jumlah kolestrol serta
fosfolipid yang rendah. Karena FFA adalah lipid yang sedang dalam
perjalanan menuju hati dan kolestrol telah meningggalkan hati, maka rasio
FFA/kolestrol dapt digunakan sebagai ekspresi lemak yang disimpan atau
dimetabolisme di dalam organ. Rasio yang tinggi pada sapi dengan hati
berlemak sesuai dengan hal ini.
Sapi-sapi yang mengalami ketosis juga memiliki kadar FFA plasma yang
tinggi tetapi jumlah kolestrol dan fosfolipid yang hampir normal serta tidak
ada atau hanya sedikit infiltrasi lemak ke dalam sel-sel hati. Dengan
demikian, sinteisis lipoprotein hanya berkurang pada tingkat yang sangat
kecil. Peningkatan rasio FFA/kolestrol disebabkan oleh oksidasi asam lemak
menjadi bahan keton. Esterifikasi asam lemak menjadi trigliserida dan
oksidasi menjadi keton merupakan dua reaksi yang cukup sering terjadi, dan
hasil penelitian ini sesuai dengan hal tersebut.
Penelitian ini juga menunjukkan bahwa akumulasi lemak dalam sel hati
dan perkembangan perlemakan hati disebabkan oleh berkurangnya sinteis
lipoprotein densitas yang sangat rendah,. Penurunan sinteis ini kemungkinan
besar terkait dengan factor makanan. Pemberian makanan prapartum yang
tinggi energi dan rendah protein terbukti merupakan factor tersebut.
Pemberian pakan ini menyebabkan kekurangan apoprotein, tetapi kekurangan
kolestrol tidak dapt dikesampingkan sebagai faktor penyebab. Pemberian
pakan biji-bijian yang tinggi dengan rasio asetat/propionate yang rendah di
dalam rumen dapat mengurangi kemungkinan sintesis kolestrol di dalam hati.
Acta vet. scand. 1989, 30, 441-445.

Plasma Lipids in Normal Cows around Partus and in Cows

with Metabolic Disorders with and without Fatty Liver
By Paul Holtenius

Department of Cattle and Sheep Diseases, Swedish University of Agricultural Sciences, Uppsala.

Holtenius, P.: Plasma lipids in normal cows around partus and in cows with meta-
bolic disorders with and without fatty liver. Acta vet. scand. 1989, 30, 441-445. -
Free fatty acids, cholesterol and phospholipids in plasma were studied from 6
weeks before to 6 weeks after calving in 16 normal multiparous cows. The same
plasma lipids were studied the day after calving in 20 normal primiparous cows.
Ten of these were fed according to standards and the other IO were overfed the last
3 weeks prepartum.
The plasma lipids were also analysed in 16 cows with left displacement of the abo-
masum and fatty liver, and in 16 cows with ketosis with no or only slight fat infilta-
tion of the liver.
In the normal cows there was a rise in FFA-level and a reduction in cholesterol and
phospholipids from 6 weeks before to the day after calving. Thereafter there was a
reduction of FFA-level and rise in cholesterol and phospholipids.
Increased feed intensity had no effect on plasma lipids at calving. The level of the
plasma lipids in cows with fatty liver differed very much from the amounts in nor-
mal cows at corresponding time from calving. Cows with ketosis had high FFA-le-
vel but the amount of cholesterol and phospholipids differed very little from nor-
mal cows.

free fatty acids; cholesterol; phosphohpids; feeding; calving; ketosis.

Introduction In cattle HDL is the major fraction compri-

In ruminants almost no plasma lipids arise
from ingested fat. Most of them are the re-
sult of de novo synthesis.
Fat is stored as triglycerides and from the de-
pots it is transported as free fatty acids
bound to albumen. A considerable part of
these acids is taken up by the liver. There
they can be oxidized to C0 2 or to ketone bo-
dies or esterified and combined with phosp-
holipids, cholesterol and apoproteins to
form lipoproteins, mainly very low density
lipoprotein (VLDL). This lipoprotein frac-
tion transports triglycerides to different or-
gans and tissues. After the loss of triglyceri-
des low density lipoproteins (LDL) are for-
med and after further metabolism high den-
sity lipoproteins (HDL).
sing more than 80% of the lipoproteins (Fer-
reri & Gleockler 1979, Holtenius & Hjort
1988) and consisting of about 60% choleste-
rol (Raphael et al. 1973). As the feed for
cattle contains very small amounts of chole-
sterol and there is high level of this substance
in plasma, there must be an important syn-
thesis in the body. Cholesterol can be syn-
thetized in the liver from acetate (Be// 1980)
and it is probably by this way the cholesterol
in the VLDL-fraction is formed. It is not
known if a de novo synthesis of HDL occurs
in the liver in cattle.
There are large variations in the fat metabo-
lism during different stages of lactation and
pregnancy and the risks of disturbances are
also variable. The intention of the present

Acta vet scand vol 30 no 4 - 1989

442 P. Holtenius

investigation was to elucidate the pathogene-
sis of fatty liver in cows by studying the in-
fluence of feeding, pregnancy and beginning
of lactation on plasma lipids in normal cows
and also by investigating plasma lipids in
cows with fatty liver and in cows with ketosis
without or with only slight fat infiltration in
the liver cells.
Material and methods
Sixteen normal multiparous Swedish Red
and White cows with an average milk yield
of 7900 kg FCM/year were studied from 6
weeks before to 6 weeks after calving. Twen-
ty normal primiparous cows of the same
breed were examined on the day after cal-
ving. Besides these normal animals 20 cows
with left displacement of the abomasum
(LDA) and fatty liver and 16 cows with keto-
sis without or with only slight fat infiltration
in the liver were examined. The cows with
LDA had been sent to the clinic for treat-
ment, and the cows with ketosis were exami-
ned on the farms.
The normal multiparous cows were fed hay,
1.5 kg DM and silage 6.5 kg DM and concen-
trates according to Swedish standards. The
first-calving cows were divided in 2 similar
groups and all animals were given the same
amount of roughage as the older cows, but
the amount of concentrates was different.
From 3 weeks before calving it was increased
gradually up to 3 kg in one group and to 7 kg
in the other group at calving. The concentra-
te was given at 8 a.m. and 2 p.m.
In all normal cows blood samples were taken
in the jugular vein at 1 p.m. 5 h after the
morning feeding and before the afternoon
were taken 1 day after the arrival to the clinic
and in the cows with ketosis sampling was
done in connection with the examination of
the animal at the farm. After sampling plas-
ma was stored at -20°C until analysed. In all
animals fine needle liver biopsy was perfor-
med for diagnosing fatty liver (Holtenius
1961, Holtenius & Niskanen 1985).
Free fatty acids, cholesterol and phospholi-
pids were determined by commercial enzy-
matic kits, NEFA C-test Wako Industries,
Osaka, Japan, for free fatty acids and Boeh-
ringer Mannhein Diagnostica for phospholi-
pids and cholesterol.
Results
The results from the study of plasma lipids
in 16 normal cows around calving are pre-
sented in Fig. 1. From 6 weeks to 2 weeks be-
fore calving there was a slight increase in
FFA from 0.13±0.05 to 0.17±0.04
mmol/l. Then there was a sharp rise to the
day after calving when the level was 0.54 ±
0.44 mmol/l. Two weeks thereafter the level
had dropped to 0.36±0.17 mmol/l and af-
ter 4 weeks to 0.30±0.17 mmol/l and after 6
weeks the amount was 0.26±0.11 mmol/l.
mmol/I mmol/I
6.0
"' s.o
:§. to
0
.c.
0.8
.c::
n.
-g 3.0 06
. u.
-e"'
.
ti 2.0 0.4

.s 1.0
0
feeding. In the multiparous cows samples 0.2
were taken every second week from 6 weeks
before to 6 weeks after calving and on the -6 -4 -2 0 2 4 6 Weeks from
day after calving. In the first calving cows c alv1ng
samples were taken on the day after calving. Figure I. Plasma lipids in 16 normal cows from 6
Samples from cows with LDA and fatty liver weeks before to 6 weeks after calving.

Acta vet scand vol 30 no 4 - 1989

 Plasma lipids in cows around partus 443

The plasma cholesterol values dropped gra-
dually from 6 weeks before to the day after
calving from 3.69±0.87 mmol/l to
2.20±0.42 mmol/l. During the following 6
weeks the level increased continuously to
4.98±1.11 mmol/l. Also the phospholipid
values dropped from 6 weeks before to mini-
mum level the day after calving. The values
were 1.83±0.49 and 1.24±0.24 mmol/l re-
0.3
0.1
-6 -4 -2 0 2 4 6 Weeks from
calving
Figure 2. Freefattyacid/cholesterolratioin l6nor-
mal cows from 6 weeks before to 6 weeks after cal-
ving.
spectively. Then the amount increased and
was 2.67 ±0.50 mmol/l 6 weeks after cal-
ving.
There was a strong rise in the FFA/choleste-
rol ratio from 2 weeks before to the day after
calving and a drop thereafter (Fig. 2). The
changes in the parameters from 6 weeks be-
fore to calving and from calving to 6 weeks
later are all significant (p < 0.01).
The results from the study of plasma lipids
in first calving cows are presented in Table 1.
The level of free fatty acids the day after cal-
ving is lower in these animals than in the ol-
der cows. The high feeding intensity had no
effect on the plasma lipids. They were very
similar in both groups.
The level of the plasma lipids in cows with
fatty liver differed very much from the
amounts in normal animals at correspon-
ding time from calving (Table 2). The cows
with fatty liver had higher level of FFA,
lower amounts of cholesterol and phospholi-

Table l. Plasma lipids the day after calving in primiparous cows on two different feed (mean±S.D.).
Number of animals lO lO
Roughage Kg DM 8 8
Concentrates kg 3 7
Metabolizable energy MJ 120 160
Digestible crude protein g 1200 1800
Free fatty acids mmol/l 0.34±0.13 0.34±0.15
Cholesterol mmol/l 2.64±0.67 2.69±0.57
Phospholipids mmol/l l.59±0.42 l.67±0.26

Table 2. Plasma lipids in cows with LOA and moderate-severe fatty liver and in cows with ketosis and no or
slight fatty liver compared with normal cows in corresponding time after calving (mean±S.D.).
Number Weeks after FFA Cholesterol Phospholipid FFA/
of cows calving mmol/l mmol/l mmol/l Cholesterol
Cows with LOA
and fatty liver 20 3-5 l.26±0.43 2.16±0.97 l.15±0.43 0.58
Normal cows 16 4 0.30±0.17 4.18±1.06 2.46±0.55 0.12
Cows with
ketosis 16 4-8 0.98±0.57 4.02±1.62 2.15±0.83 0.24
Normal COWS 16 6 0.26±0.ll 4.98±1.ll 2.67±0.50 0.05

Acta vet scand vol 30 no 4 - 1989

444 P. Holtemus
pids and higher FFA/cholesterol ratio. The
level of FFA was also higher in cows with ke-
tosis than in normal animals but the diffe-
rences in cholesterol and phospholipids were
very small.
Discussion
During the period around calving there are
big changes in the level of plasma lipids. In
spite of increased feeding intensity in the last
weeks before calving there is a strong rise in
the FFA-level. The experiment with one very
high and one moderate feeding intensity to
first calving cows shows, that the rise in FFA
is not inhibited by increased feeding. There
are many reports on fat metabolism during
the end of pregnancy and the post parturient
period especially in laboratory animals but
also in cows (McNamara & Hillers 1986).
There is no doubt that the change of the fat
metabolism is initiated by pregnancy and
lactogenic hormones. Several investigators
have emphasized the importance of prolac-
tin in this connection (Bauman & Currie
1980, Agius et al. 1979, Zinder et al. 1974). ·
Influence of reduced progesterone activity
on lipoprotein lipase activity has been stu-
died by Spooner et al. 1977, and on the num-
ber of insulin receptors in adipose tissue by
Vernon et al. 1981.
There are two main reasons for increased li-
polysis in adipose tissue and rised level of
free fatty acids in plasma, the hormonally
induced at partus and lack of energy in the
feed. In both these cases there is increased
transport of fatty acids to the liver and in-
creased demand for capacity to eliminate
them. There are also two main ways for eli-
mination namely esterification to triglyceri-
des to form a part in lipoprotein or oxidation
to ketone bodies, the choice of which will de-
pend on the glucose metabolism. If there is
increased fatty acid transport to the liver and
Acta vctt scand vol 30 no 4 - 1989
the elimination is reduced, the result will be
accumulation of triglycerides in the cells.
The present investigation shows that the
cows with fatty liver had very high plasma
FFA-level and low amounts of cholesterol
and phospholipids. This indicates that the
synthesis of lipoproteins is reduced. As FFA
are lipids on the way to the liver and chole-
sterol has left the liver, the FF,A/cholesterol
ratio can be used as an expression of fat re-
tained or metabolised in the organ. The high
ratio in the cows with fatty liver is in accor-
dance with this.
The cows with ketosis also had high plasma
FFA-level but almost normal amounts of
cholesterol and phospholipids and no or on-
ly slight fat infiltration of the liver cells.
Thus the synthesis of lipoproteins was redu-
ced only to a very small degree. The increa-
sed FFA/cholesterol ratio is caused by the
oxidation of fatty acids to ketone bodies.
Esterification of fatty acids to triglycerides
and oxidation to ketones are two quite rever-
se reactions, and the results from the present
investigation are in agreement with this.
The studies also show that accumulation of
fat in the liver cells and development of fatty
liver is caused by reduced synthesis of very
low density lipoprotein, VLDL. The reduced
synthesis is most probably associated with
feeding factors. High energy - low protein
prepartum feeding is shown to be such a fac-
tor (Holtenius & Hjort 1988). One possibili-
ty is that this feeding leeds to lack of apopro-
tein, but lack of cholesterol can not be exclu-
ded as a causal factor. High grain feeding re-
sulting in low acetate/propionate ratio in the
rumen may reduce the possibilities for chole-
sterol synthesis in the liver.
 Plasma lzpids zn cows around partus
References
Ag1us L, Robinson AM, Girard JR, W1ll1amson DH:
Alterations in rate of lipogenes1s in vivo in mater-
nal liver and adipose tissue on premature wea-
ning of lactating rats. Biochem. J. 1979, 180,
689-692.
Bauman DE, Currie WB: Partitioning of nutrients
durmg pregnancy and lactation: A review of me-
chanisms involvmg homeostaus and homeorhe-
s1s. J. Dairy Sci. 1980, 63, 1514-1529.
Bell A W: Lipid metabolism m liver and selected tis-
sues and in the whole body of ruminant ammals.
Proc. Lipid Res. 1980, 18, 117-164.
Ferreri LF, Gleockler DH: Electrophoretic characte-
rization of bovme lipoprotein subfractions isola-
ted by agarose gel chromatography. J. Dairy Sci.
1979, 62, 1577-1582.
Holtemus P: Cytological puncture: A new method
for the study of bovine hepatic disease. Cornell
Vet. 1961, 50, 56-63.
Holtemus P, H1ort M: Studies on the pathogenesis of
fatty hver m cows. Proc. XV World Buiatnc
Congress 1988, 214-220.
Holtemus P, Niskanen R: Leberzellverfettung bei
Kuhen mit Labmagenverlagerung. (Fatty chan-
ges of the liver cells in cows with abomasal displa-
cement). Dtsch. Tierarzl. Wschr. 1985, 92, 398-
400.
McNamara JP, Hillers JK: Adaptations m lipid me-
tabolism of bovine adipose tissue in lactogenesis
and lactation. J. Lipid Res. 1986, 27, 150-157.
Raphael BC, D1m1ck PS, Pupp1one DL: Lipid cha-
racterization of bovine serum lipoproteins
throughout gestation and lactation. J. Dairy Sci.
1973, 56, I025-I032.
Spooner PM, Garrison MM, Scow RO: Regulation
of mammary and adipose tissue lipoprotein lipa-
se and blood triacylglycerol m rats during late
pregnancy. Effect of prostaglandins. J. clin. In-
vest. 1977, 60, 702-708.
(Accepted February 9, 1989).
Reprints may be requested from: Paul Holtenius, Dept. of Cattle and Sheep Diseases, Swedish University of
Agricultural Sciences, P. 0. Box 7019, S-75007 Uppsala, Sweden.
445
Vernon RG, Clegg RA, Flint DJ: Metabolism of
sheep adipose tissue during pregnancy and lacta-
tion. Adaptation and regulation. Biochem. J.
1981, 200, 307-314.
Zinder 0, Hamosh M, Fleck C, Scow R: Effect of
prolactm on lipoprotein lipase in mammary
gland and adipose tissue of rats. Amer. J. Phy-
siol. 1974, 226, 744-748.
Sammanfattning
Plasmallp1der hos normala kor runt partus och hos
kor med amnesomsattningsrubbningar med och
utan leverforfettning.
Fria fettsyror, kolesterol och fosfolipider i plasma
studerades fn\n 6 veckor fore till 6 veckor efter par-
tus hos 16 hogproducerande normala kor, som kal-
vat mer an en gang. Samma plasmalipider studera-
des dagen efter kalvningen pa 20 normala forsta-
kalvskvigor av vilka IO fatt normal utfodring och IO
overutfodrats fram til partus.
Plasmahpiderna undersoktes ocksa pa 16 kor med
vanstersidig lopmagsforskjutning och leverforfett-
ning och pa 16 kor med acetonami och ingen eller en-
dast ringgradig leverforfettning.
Hos de normala korna steg mangden fria fettsyror
fram till kalvningen och sjonk darefter. Kolesterol
och fosfolipider sjonk fram till kalvningen och steg
darefter. FFA/kolesterolkvoten steg och var hogst
dagen efter kalvningen.
Det var ingen sk11Inad 1plasmalip1der mellan de bada
grupperna forstakalvskvigor. Den kraftigt okade fo-
dertillforseln paverkade inte stegringen av FFA.
Hos kor med Ieverforfettning avvek mangderna av
de olika plasmahp1derna mycket tydligt fran koncen-
trationen hos fnska kor v1d motsvarande tidpunkt
efter kalvnmgen.
Kor med acetonami bade hogre mangd FFA an nor-
mala kor men betraffande kolesterol och fosfolipi-
der var skillnaderna mycket sma.
Acta vet scand vol 30 no 4 - 1989