Lampiran 1.

Komposisi Media dan Reagen

N
MEDIA SATUAN JUMLAH
O
1. Media CASO 0,2 g 0,2 x 2 = 0,4 g
Pancreatic digest of casein 0,75 g
Enzymatic digest of soya bean 0,025 g
Sodium chloride 0,0255 g
Agar 0,075 g
2. Media BHI 0,1 g 0,1 x 4 = 0,4 g
Brain infusion solid 0,375 g
Beef heart infusion solid 0,015 g
Pentose peptone 0,03 g
Sodium chloride 0,015 g
Glucose 0,006 g
Disodium phosphate 0,0075 g
Agar 0,03 g
3. Media NA miring 0,2 g 0,2 x 2 = 0,4 g
4. Media MHA 0,57 g
a. Beef ekstrak 0,9 g
b. Casein hydrolysate 0,2625 g
c. Starch 0,0225 g
d. Agar 0,0255 g
5. Media MC 0,78 g 0,78 x 2 = 1,56 g
Peptone 0,3 g
Lactose 0,15 g
Bile salts 0, 075 g
Sodium chloride 0,075 g
Neutral red 0,00125 g
Agar 0.18135 g
6. TSIA 0,325 g 0,325 x 1 = 0,325 g
Lab-Lemc Powder 0,015 g
Yeast extract 0,015 g
Peptone 0,1 g
Sodium chloride 0,025 g
Lactose 0,05 g
Sucrose 0,05 g
Glucose 0,005 g
Ferric citrate 0,0015 g
Sodium thiosulphate 0,0015 g
Phenol red 0,00012 g
Agar 0,06 g
7. UREA 0,075 g 0,075 x 1 = 0,075 g
Peptone 0,003 g
Glucose 0,003 g
Sodium chloride 0,015 g
Disodium phosphate 0,0036 g
Potassium dihydrogen phosphate 0,024 g
Phenol red 0,000036 g
 Agar 0,045 g
8. SIM 0,09 g 0,09 x 2 = 0,18 g
Tryptone 0,06 g
Peptone 0,0183 g
Ferrous ammonium sulphate 0,0006 g
Sodium thiosulphate 0,0006 g
Agar 0,0105 g
9. MR/VP 0,051 g 0,051 x 2 = 0,102 g
Peptone 0,021 g
Glucose 0,015 g
Phosphate buffer 0,015 g
10. Simmons citrate 0,115 g 0,115 x 1 = 0,115 g
MgSo4 0,001 g
Ammonium dihydrogen phosphate 0,001 g
Sodium ammonium phosphate 0,004 g
Sodium citrate 0,01 g
Sodium chloride 0,025 g
Bromothymol blue 0,0004 g
Agar 0,075 g
11. Gula-gula
a. Glukosa 0,425 g
Pepton water 0,375 g
Glukosa 0,05 g
b. Laktosa 0,425 g
Pepton water 0,375 g
Laktosa 0,05 g
c. Maltosa 0,425 g
Pepton water 0,375 g
Maltose 0,05 g
d. Manitol 0,425 g
Pepton water 0,375 g
Mannitol 0,05 g
e. Sakarosa 0,425 g
Pepton water 0,375 g
Sakarosa 0,05 g
12. Reagen standar Mc. Farland
a. BaCl2 0,5 ml 0,5 x 2 = 1 ml
b. H2SO4 99,5 ml 99,5 x 2 = 199 ml
13. Cat Gram
a. Gram A
Lart 1 : 1) Kristal violet 0,2 gr 0,2 x 2 = 0,4 gr
2) Alkohol 20 ml 20 x 2 = 40 ml
3) Ammonium oksalat 0,8 gr 0,8 x 2 = 1,6 gr
4) Aquadest 80 ml 80 x 2 = 160 ml
b. Gram B
1) Iodium 1 gr 1 x 2 = 2 gr
2) Kalium iodide 2 gr 2 x 2 = 4 gr
3) Aquadest 300 ml 300 x 2 = 600 ml
 c. Gram C
Aceton 50 ml 50 x 2 = 100 ml
Alkohol 50 ml 50 x 2 = 100 ml
d. Gram D
1) Safranin 0.25 gr 0,25 x 2 = 0,5 gr
2) Alkohol 96% 10 ml 10 x 2 = 20 ml
3) Aquadest 90 ml 90 x 2 = 180 ml
13. Reagen Uji Biokimia
a. Reagen Kovac
Kovac’s Indole reagent 100 ml
Tryptone water 50 g
b. Methyl Red
Methyl red 0,1 g
Ethyl alcohol 95% 300 ml
Aquades 200 ml
c. KOH 40%
Potassium hydroxide 40 g
Aquades 100 mll
d. FeCl3 10%
FeCl3 10 g
Aquades 100 ml
e. Barried
Solution A
° Alpha naphtol 5g
° Etanol absolut 95 g
Solution B
° Potassium hydroxide 40 g
° Creatine 0,3 g
° Aquades 1000 ml