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Diterbitkan oleh :
YAYASAN HAMJAH DIHA
Alamat Bima : Jln. Lintas Parado, Desa Tangga Kecamatan Monta Kabupaten Bima – NTB
Alamat lombok : Jln. TGH. Badaruddin, Blok D no. 5 BTN KUBAH HIJAU, BAGU Pringgarata – Lombok
Tengah
Email :kontak@hamjahdiha.or.id
Website.hamjahdiha.or.id

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BIOKIMIA D3/S1 FARMASI
 BUKU PEDOMAN PRAKTIKUM
BIOKIMIA D3/S1 FARMASI
Copyright@Yayasan Hamjah Diha, 2021
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Hak cipta dilindungi oleh undang – undang

All right reserved

Cetakan Perdana

Penulis :
Faizul Bayani, S.Pd., M.Pd
Dodiy Firmansyah, S.Si., M.Si
Meilinda Pomeistia, S.Pd., M.Pd

Editor : Dodiy Firmansyah, S.Si., M.Si

Lay Out : Mujakir
Design Cover : Mujakir

Diterbitkan oleh :
YAYASAN HAMJAH DIHA
ANGGOTA IKAPI
Alamat Bima : Jln. Lintas Parado, Desa Tangga Kecamatan Monta Kabupaten Bima – NTB
Alamat lombok : Jln. TGH. Badaruddin, Blok D no. 5 BTN KUBAH HIJAU, BAGU Pringgarata – Lombok
Tengah
Email :kontak@hamjahdiha.or.id

BUKU PEDOMAN PRAKTIKUM

BIOKIMIA D3/S1 FARMASI
 KATA PENGANTAR
Puji syukur kami panjatkan kepada Allah SWT yang dengan
karuniaNYA sehingga kami diberikan kemudahan dalam penyusunan buku Page | iv
Pedoman Praktikum Biokimia Farmasi untuk D3 dan S1. Maksud
penyusunan Pedoman ini adalah untuk membantu mahasiswa dalam
melaksanakan praktikum yang menunjang pemahaman terhadap teori
Biokimia Farmasi yang diberikan dalam perkuliahan. Materi praktikum
meliputi analisis/karakterisasi karbohidrat, protein, enzim, lipid, dan DNA.
Materi-materi tersebut disusun berdasarkan literatur sebagai bahan acuan.
Dalam Pedoman ini hanya diberi beberapa contoh, sehingga mahasiswa
masih perlu mencari dan mempelajari literatur lain sebagai pendukung.
Kami menyadari bahwa dalam penyusunan buku Pedoman ini tentu masih
ada kekurangan, sehingga kami membutuhkan saran, kritik dan masukan
dalam penyusunan dan revisi buku ini selanjutnya. Kami berharap semoga
buku ini bermanfaat.

Bagu, Juli 2021

Tim Penyusun
BUKU PEDOMAN PRAKTIKUM
BIOKIMIA D3/S1 FARMASI
 DAFTAR ISI
KATA PENGANTAR ....................................................................... iv
DAFTAR ISI ....................................................................................... v Page | v
I. TATA TERTIB ........................................................................ vi
II. SANKSI ..................................................................................... vi
PANDUAN PENYUSUNAN LAPORAN........................................ vi
EVALUASI PRAKTIKUM .............................................................. vi
PANDUAN PENILAIAN .................................................................. x
PERCOBAAN I .................................................................................. 1
ANALISIS KUALITATIF KARBOHIDRAT ................................. 1
A. LANDASAN TEORI ............................................................... 1
B. TUJUAN .................................................................................... 1
C. ALAT DAN BAHAN ................................................................ 1
D. PROSEDUR KERJA ................................................................ 2
E. LEMBAR KERJA .................................................................... 2
DAFTAR PUSTAKA ......................................................................... 4
PERCOBAAN II ................................................................................ 5

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ANALISIS KUANTITATIF KARBOHIDRAT ............................. 5
A. PENDAHULUAN .................................................................... 5
B. TUJUAN .................................................................................... 5 Page | vi
C. ALAT DAN BAHAN ................................................................ 5
D. CARA KERJA .......................................................................... 6
E. LEMBAR KERJA .................................................................... 6
DAFTAR PUSTAKA ......................................................................... 6
PERCOBAAN III ............................................................................... 7
ANALISIS GLIKOGEN .................................................................. 7
A. PENDAHULUAN ..................................................................... 8
B. TUJUAN .................................................................................... 8
C. ALAT DAN BAHAN ................................................................ 8
D. CARA KERJA .......................................................................... 8
E. LEMBAR KERJA .................................................................... 8
DAFTAR PUSTAKA ......................................................................... 8
PERCOBAAN IV ............................................................................... 9
ANALISIS KUALITATIF LIPID .................................................... 9

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BIOKIMIA D3/S1 FARMASI
A. LANDASAN TEORI ................................................................ 9
B. TUJUAN .................................................................................. 10
C. ALAT DAN BAHAN .............................................................. 10 Page | vii
D. PROSEDUR KERJA .............................................................. 10
E. LEMBAR KERJA .................................................................. 11
DAFTAR PUSTAKA ....................................................................... 11
PERCOBAAN V .............................................................................. 12
ANALISIS KUANTITATIF LIPID ............................................... 12
A. LANDASAN TEORI .............................................................. 12
B. TUJUAN .................................................................................. 12
C. ALAT DAN BAHAN .............................................................. 12
D. PROSEDUR KERJA .............................................................. 12
E. LEMBAR KERJA .................................................................. 13
DAFTAR PUSTAKA ....................................................................... 13
PERCOBAAN VI ............................................................................. 14
PENENTUAN ANGA PENYABUNAN ......................................... 14
A. LANDASAN TEORI .............................................................. 14

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BIOKIMIA D3/S1 FARMASI
B. TUJUAN .................................................................................. 14
C. ALAT DAN BAHAN .............................................................. 14
D. PROSEDUR KERJA .............................................................. 14 Page | viii
E. LEMBAR KERJA .................................................................. 15
DAFTAR PUSTAKA ....................................................................... 15
PERCOBAAN VII ........................................................................... 16
ANALISIS KUALITATIF PROTEIN ........................................... 16
A. LANDASAN TEORI .............................................................. 16
B. TUJUAN .................................................................................. 17
C. ALAT DAN BAHAN .............................................................. 17
D. PROSEDUR KERJA .............................................................. 18
E. LEMBAR KERJA .................................................................. 19
DAFTAR PUSTAKA ....................................................................... 19
PERCOBAAN VIII .......................................................................... 20
PENGENDAPAN DAN PENENTUAN KADAR PROTEIN...... 20
A. DASAR TEORI ....................................................................... 20
B. TUJUAN PERCOBAAN ........................................................ 21

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C. ALAT DAN BAHAN .............................................................. 21
D. CARA KERJA ........................................................................ 21
E. LEMBAR KERJA .................................................................. 22 Page | ix
DAFTAR PUSTAKA ....................................................................... 22
PERCOBAAN IX ............................................................................. 24
ANALISIS KUALITATIF VITAMIN ........................................... 24
A. LANDASAN TEORI .............................................................. 24
B. TUJUAN .................................................................................. 24
C. ALAT DAN BAHAN .............................................................. 24
D. PROSEDUR KERJA .............................................................. 25
E. LEMBAR KERJA .................................................................. 27
DAFTAR PUSTAKA ....................................................................... 27
PERCOBAAN X .............................................................................. 28
ANALISIS KUANTITATIF VITAMIN ........................................ 28
A. PENDAHULUAN ................................................................... 28
B. TUJUAN .................................................................................. 28
C. ALAT DAN BAHAN .............................................................. 28

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D. CARA KERJA ........................................................................ 29
E. LEMBAR KERJA .................................................................. 30
DAFTAR PUSTAKA ....................................................................... 30 Page | x
PERCOBAAN XI ............................................................................. 31
ISOLASI DNA DAN ELEKTROFORESIS GEL AGAROSA ... 31
A. LANDASAN TEORI .............................................................. 31
B. TUJUAN .................................................................................. 31
C. ALAT DAN BAHAN .............................................................. 31
D. PROSEDUR KERJA .............................................................. 32
E. LEMBAR KERJA .................................................................. 34
DAFTAR PUSTAKA ....................................................................... 34
PERCOBAAN XII ........................................................................... 35
ENZIM .............................................................................................. 35
A. LANDASAN TEORI .............................................................. 35
B. TUJUAN .................................................................................. 35
C. ALAT DAN BAHAN .............................................................. 35
D. PROSEDUR KERJA .............................................................. 36

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BIOKIMIA D3/S1 FARMASI
E. LEMBAR KERJA .................................................................. 38
DAFTAR PUSTAKA ...................................................................... 38
RESPONSI/UJIAN .................................................................. 39 Page | xi
LAMPIRAN ...................................................................................... 40

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BIOKIMIA D3/S1 FARMASI