3A

MID LAB

TASK 1:
Step 1
Open UniprotKB and search for myoglobin

Step 2
Open the First Result

Step 3
Record it’s Accession Number
Accession Number: P02144
Step 4
Click on sequence on the left Panel in the website
Step 5
Click on any of the sequence

It will open on ebi.ac.uk

Step 6
View as FASTA
FASTA: >ENA|X00371|X00371.1 Human myoglobin gene (exon 1) (and joined CDS)
CCTCTGACCCTTTTGGTCGCTAGGAGTCAGCCGACTCAGTACACAGGACTCACTGAATGG
AGACACAAGGCTCCTCCAGGGAGTGGCGGCTCATGGCAATCCTAGAATGGTCACCAGCCA
GGCTTTAGAGACCCACACAGAGGGCGTTCTGACCCAAAGTTGCACTGGGGAACTCCAAGT
TTGGGGATTCTTTGAATTTAACTCTTTTTCTAGCTACATTTCCTATTATTTGTCCAATTC
TTACCAAACATCTCTGTTCACATTCTGAAGCTGGGATCTGACTGGCAGAGCTAGTAGATG
CTGACTATTCAGATGGAGCCCTGACATTGGCTTTCTCAGCTTGGCTGTGACTGGCAGCAG
GTTTGCGGGAGAACTGTGTGTCCCAGAACATGACTGGCTACACCTGCACCTCAGCAAGAT
TGGGGCAGGGCAGTTATCTTCAAAAAGCTGTGTAGGTGGGGCAGTCATTACTGACAAATC
CAGTGCAGACCCAGGATGGCCCAAACACTGGCTTATCCTTTCTGAATCTCATCTCCCACA
GCTGTAAAGCGGGGTGGTGCTCGCTACCTCACAGAGGTGTTGTAAAGATTAGATGTAATC
TTGCCAAGCAGCCACTTTGTAAACTGTATAGTCTTATGCAGATGGAAGGAAGGGCCTGTG
CCTACCTTGATCATAGCACTAAACAAACTGTACTGTATTTTCATTCCTCTTAGTTATCTC
CCTAAAAAGACTCTGAGTTCCTTGAACACAGGAAGGTGTTTTATTTGATTTTGTTATCCT
CAGCATGTAGCAGTGTCTGACACACAGTAGGTGCTCTATCACTGTGAGAGGGATGGATGG
ATGGGTGGAGTTACAGATGGATAGAAGGATAGATGGAGGGATGGGTGGATGATGGATGGA
TAGATGGATGGAGGGGGGATGATGAATGGAGGGATAATGAGTGGATGAATGAGGGAATGG
GTGGATGGATGGATGGAGGGATGGAGGAACAGATAGATAGATGGAGGGATGGGTGGGTGA
TGGATGGATAGATGGATGGAGGGAGGGATGATGAATGGAGGGATAATGAATGGATGAATG
AGGGGATGGGTGGATGGATGAATGGAGGGATGATGGGTGGATGAATGAATTGAGGGATGG
ATGGATGAACACATGGATGGATGGATAGATGGATAGATGGAGGAACTGGTGGATTTTGGA
TGGATGGGTGGATGGATAGATGAATGAATGCCTGGATAGACAAAGAGATGATGGATAGAT
GAATAGATGAATTAAGGGATGTCGGATAGATGGAGGGATTGATAGATGTTGGATGGATGG
GTGGTGGATGGATAGATGAGTGAATGCATGGATAGACAAAGAGATGATGGATGGATGAAT
TAAGGGATGACAGATGGATGGATGGATGAGTAACTGGATGGACAAGTGGATAAATGGATA
GATGGTTGAATACCTGAATGGATTGAAGGAGGATGCATGGATGTAAGATAAGGCTAATCA
TCCTCCACTCTCTTTCTTTGCAAAACCATCCACCCATTTACTCAATAAACATTTATTCAG
TTCAAACTTGGCACAAAGCACCATGTGAGGCCCAAGAGATACGTGGGTTAATAAAACAGA
GCTCCTGCCCTCCTGAAAACTGCAAAGAAAGGGGCGTGGCTTCCTGAGTTCAAATCCCAA
CTCTGCCAGCGACTAGCTGTACATCAGTGATGTTTCCCTACTTTCTCTCAATTAAATAGG
GATAATGTCAGTACCTATCACATTGGGAGGTCTTGCGGGGATTAAATGAGTTACCAAATG
CCAAGTGTTTGGGACAGGGCCTGGCACCCAGCAAAGTCTCTTGTGAGTGCTGGCTGCTAT
TATCCTAATGGAGAAGATGGCATGAAAACCAGGAAATAGGATGCCCTTTGGGAAGCAATG
CAACAGGAACTTACACAAAGAAAGGAAAGGAGGAAGCAATTAGTGGTGTCTCAAAGGAGT
ATGTCAAGAAAAACTTTTCAGAGGGAAACCTTTGAGCAGGGCCATGAAAACAGGAGTTCT
CTAAGAGATTGTGGACTTGCCTGGGACCACCTGGCTATAAGCACAAAACCATCCGGTTCC
TTTCTGTCACTTCTGGCGGGTGAGGGGTCTCTGGCAAAGGGGCAGAAGGTGCGTGAGAGG
TTGCGAATGGCAGGACTGTCCTGGCCAGCCGGGGCACCTGGTGGCCAAGCTTAGAAACAT
GACAGGTCCTCTTGGGAGGGCTGACCGCAGGGAGCGTTGGGTTTCAGGCTGCTGGCGTCG
GCTTCTGTGGTGCCCTTTCTGTCGGCTATGAGAGTCCAGACAGTGCCCAACCTCCTCCCC
TTCTTTCCACACGCACAACCACCCCACCCCCTGTGGCCTGAGCTGTCCTGCCTCGCCACA
ATGGCACCTGCCCTAAAATAGCTTCCCATGTGAGGGCTAGAGAAAGGAAAAGATTAGACC
CTCCCTGGATGAGAGAGAGAAAGTGAAGGAGGGCAGGGGAGGGGGACAGCGAGCCATTGA
GCGATCTTTGTCAAGCATCCCAGAAGGTATAAAAACGCCCTTGGGACCAGGCAGCCTCAA
ACCCCAGCTGTTGGGGCCAGGACACCCAGTGAGCCCATACTTGCTCTTTTTGTCTTCTTC
AGACTGCGCCATGGGGCTCAGCGACGGGGAATGGCAGTTGGTGCTGAACGTCTGGGGGAA
GGTGGAGGCTGACATCCCAGGCCATGGGCAGGAAGTCCTCATCAGGTAAAAGGAAGAGAT
TCCATTGCCCCTGCCACCCACACCCTAAGATCAAGGGTGTTCAGCTGCAAGGTGGAAAGT
TTGCACGTGGGGTAGGTCAGTTGGCTGCATTAGTTAAGGGTGTTAGAACGGTCACTTGCT
TTTTCTTTGCTTTTAAGTGTCAGGGATTGGACTCAGGAGAGGGAAAGGAGCCATTTCAGG
CTGATATCAGCAGCTGGAGGAAGCATGAGAATCAAACCTAGGATGCTCAGAGTCCACCAG
GAAGAATTTTAGAATTATAGACAGTCAGAGTTAACAAGGGTCCTGAGAGATTTTGTACAG
CCACCTCTCTTACAGGATGAGGACAAAAAGCGACTGAGAAGGGGAGGACATTTCCAGAGT
CACAGCTCATTAAATGCTCTTAAAGTGTCAAGGTTAAGACATGCTCTTCAAGGGGAGACA
GATCTGGTTCTAGACTTGGCTCTGCCACTGAGCCACTGGGTGACCTTTGGGAAGGTACTC
AACCTCTCGGAGCCTCAATTTCCTCTCCTGTACAGTGAGGGGATATCCTAATATCTATAT
CCTAGAGGAGATGTGAGAATTAAATAAAATAATGCATGCAAGAGGCCTGGCATGGTTCCT
GGCATATACTGAGTCCTAGAAATGTTAGTAGCTATTACTGATGAAGCCCAGGCTAGGGAC
CTTTCAAAGCATTGCAATTAGAGAACAGAAGATAGAGGCTCATTAGTGACCTTCGATGTT
GAGTATGTCTCTAGTTTGAGAGGTCTGAATGATGTGGTCTGCAAGTATATCCTGCCTTCT
ACCACAAGGGATTCCAGAATACACCAAAGAAAACAAAATTCTGAGGTTTGTAAATAGAGG
GTGGCTGTGGTTTGTACATAGAAGCTCATCTCCTCGTTGCCTTCTATCCCAAAGGTGATA
CACTCTTCTCTTGGCCCCTTCCCTCACCATTCTGAGCTGGTTCCCTCAGAAGTCTAATAG
GTTAAGAATCAACGTTTCTGCCAACGGGAGGAAGGAAGTGGGCGCCGG

TASK 2:
Step 1
Search A5A6J8 in UNIPROTKB
Step 2
Click on Sequence on the left panel of the website and click on the SEQUENCE

It will open on ebi.ac.uk

VIEW as FASTA

FASTA: >ENA|AB222126|AB222126.1 Pan troglodytes verus atp1b1 mRNA for

ATPase, Na+/K+ transporting, beta 1 polypeptide, transcript variant 1,
complete cds, clone: PorA0666.
CTGCCTGCAGAGAGCCAGGCCGGAGAAGCCGAGCGGCGCAGAGGACGCCAGGGCGCGCGC
CGCAGCCACCCACCCTCCGGACCGCGGCAGCTGCTGACCCGCCATCGCCATGGCCCGCGG
GAAAGCCAAGGAGGAGGGCAGTTGGAAGAAATTCATCTGGAACTCAGAGAAGAAGGAGTT
TCTGGGCAGGACCGGTGGCAGTTGGTTTAAGATCCTTCTATTCTACGTAATATTTTATGG
CTGCCTGGCTGGCATCTTCATCGGAACCATCCAAGTGATGCTGCTCACCATCAGTGAATT
TAAGCCCACATATCAGGACCGAGTGGCCCCGCCAGGATTAACACAGATTCCTCAGATCCA
GAAGACTGAAATTTCCTTTCGTCCTAATGATCCCAAGAGCTATGAGGCATATGTGCTGAA
CATAGTTAGGTTCCTGGAAAAGTACAAAGATTCAGCCCAGAGGGATGACATGATTTTTGA
AGATTGTGGTGATGTGCCCAGTGAACCGAAAGAACGAGGAGACTTTAATCATGAACGAGG
AGAGCGAAAGGTCTGCAGATTCAAGCTTGAATGGCTGGGAAATTGCTCTGGATTAAATGA
TGAAACTTATGGCTACAAAGAGGGCAAACCATGCATTATTATAAAGCTCAACCGAGTGCT
AGGCTTCAAACCTAAGCCTCCCAAGAATGAGTCCTTGGAGACTTACCCAGTGATGAAGTA
TAACCCAAATGTCCTTCCCGTTCAGTGCACTGGCAAGCGAGATGAAGATAAGGATAAAAT
TGGAAATGTGGAGTATTTTGGACTGGGCAACTCCCCTGGTTTTCCTCTGCAGTATTATCC
CTACTATGGCAAACTCCTGCAGCCCAAATACCTGCAACCCCTGCTGGCCGTACAGTTCAC
CAATCTTACCATGGACACTGAAATTCGCATAGAGTGTAAGGCGTACGGTGAGAACATTGG
GTACAGTGAGAAAGACCGTTTTCAGGGACGTTTTGATGTAAAAATTGAAGTTAAGAGCTG
ATCACAAGCACAAATCTTTCCCACTAGCCATTTAATAAGTTAAAAAAAGATACAAAAACA
AAAACCTACTAGTCTTGAACAAACTGTCATACGTATGGGACCTACACTTAATCTATATGC
TTTACACTAGCTTTCTGCATTTAATAGGTTAGAATGTAAATTAAAGTGTAGCAATAGCAA
CAAAATATTTATTCTACTGTAAATGACAAAAGAAAAAGAAAGATTGAGCCTTGGGACGTG
CCCATTTTTACTGTAAATTATGATTCCATAACTGACTTGTAGTAAGCAGTGTTTCTGGCC
CCTAAGTATTGCTGCCTTGTGTATTTTATTTAGTGTACAGTACTACAGGTGCATACTCTG
GTCATTTTTCAAGCCATGTTTTATCGTATCTGTTTTCTACTTTATGTGAGCAAGGTTTGC
TGTCCGAGGTGTAAATATTCAACGGGAATAAAACTGGCATGGTACTTTTTTTTTTTTTTT
TGGCTCTTTCAAAGGTAATGGCCCATCGATGAGCATTTTTAACATACTCCATAGTCTTTT
CCTGTGGTGTTAGGTCTTTATTTTTATTTTTTTCCTGGGGGCTGGGGTGGGGGTTTGTCA
TGGGGGAACTGCCCTTTAAATTTTAAGTGACACTACAGAAAAACACAAAAAGGTGATGGG
TTGTGTTATGCTTGTATTGAATGCTGTCTTGACATCTCTTGCCTTGTCCTCCGGTATGTT
CTAAAGCTGTGTCTGAGATCTGGATCTGCCCATCACTTTGGCTAGTGACAGGGCTAATTA
ATTTGCTTTATACATTTTCTTTTACTTTCCTTTTTTCCTTTCTGGAGGCATCACATGCTG
GTGCTGTGTCTTTATGAATGTTTTAACCATTTTCATGGTGGAAGAATTTTATATTTATGC
AGTTGTACAATTTTATTTTTTTCTGCAAGAAAAAGTGTAATGTATGAAATAAACCAAAGT
CACTTGTTTGAAAATAAATCTTTATTTTGAACTTTATAAAAAGCAATGCAGGTACCCCAT
AGACTGGTGTTAAATGTTGTCTACAGTGCAAAATCCATGTTCTAACATATGTAATAATTG
CCAGGAGTACAGTGCTCTTGTTGATCTTGTATTCAGTCAGGTTAAAACAATGGACAATAA
AAGAATGAACACAAAAAAAAAAAAAAAAAA

TASK 3
Step 1
Go to BLAST an enter accession id’s of dna and mRNA
And click on BLAST.

Step 2
Download the aligned sequence
FASTA: >AB222126.1:1-2190 Pan troglodytes verus atp1b1 mRNA for ATPase, Na+/K+ transporting,
beta 1 polypeptide, transcript variant 1, complete cds, clone: PorA0666

CTGCCTGCAGAGAGCCAGGCCGGAGAAGCCGAGCGGCGCAGAGGACGCCAGGGCGCGCGCCGCAGCCACC

CACCCTCCGGACCGCGGCAGCTGCTGACCCGCCATCGCCATGGCCCGCGGGAAAGCCAAGGAGGAGGGCA

GTTGGAAGAAATTCATCTGGAACTCAGAGAAGAAGGAGTTTCTGGGCAGGACCGGTGGCAGTTGGTTTAA

GATCCTTCTATTCTACGTAATATTTTATGGCTGCCTGGCTGGCATCTTCATCGGAACCATCCAAGTGATG

CTGCTCACCATCAGTGAATTTAAGCCCACATATCAGGACCGAGTGGCCCCGCCAGGATTAACACAGATTC

CTCAGATCCAGAAGACTGAAATTTCCTTTCGTCCTAATGATCCCAAGAGCTATGAGGCATATGTGCTGAA

CATAGTTAGGTTCCTGGAAAAGTACAAAGATTCAGCCCAGAGGGATGACATGATTTTTGAAGATTGTGGT

GATGTGCCCAGTGAACCGAAAGAACGAGGAGACTTTAATCATGAACGAGGAGAGCGAAAGGTCTGCAGAT

TCAAGCTTGAATGGCTGGGAAATTGCTCTGGATTAAATGATGAAACTTATGGCTACAAAGAGGGCAAACC

ATGCATTATTATAAAGCTCAACCGAGTGCTAGGCTTCAAACCTAAGCCTCCCAAGAATGAGTCCTTGGAG

ACTTACCCAGTGATGAAGTATAACCCAAATGTCCTTCCCGTTCAGTGCACTGGCAAGCGAGATGAAGATA

AGGATAAAATTGGAAATGTGGAGTATTTTGGACTGGGCAACTCCCCTGGTTTTCCTCTGCAGTATTATCC

CTACTATGGCAAACTCCTGCAGCCCAAATACCTGCAACCCCTGCTGGCCGTACAGTTCACCAATCTTACC

ATGGACACTGAAATTCGCATAGAGTGTAAGGCGTACGGTGAGAACATTGGGTACAGTGAGAAAGACCGTT

TTCAGGGACGTTTTGATGTAAAAATTGAAGTTAAGAGCTGATCACAAGCACAAATCTTTCCCACTAGCCA

TTTAATAAGTTAAAAAAAGATACAAAAACAAAAACCTACTAGTCTTGAACAAACTGTCATACGTATGGGA

CCTACACTTAATCTATATGCTTTACACTAGCTTTCTGCATTTAATAGGTTAGAATGTAAATTAAAGTGTA

GCAATAGCAACAAAATATTTATTCTACTGTAAATGACAAAAGAAAAAGAAAGATTGAGCCTTGGGACGTG
CCCATTTTTACTGTAAATTATGATTCCATAACTGACTTGTAGTAAGCAGTGTTTCTGGCCCCTAAGTATT

GCTGCCTTGTGTATTTTATTTAGTGTACAGTACTACAGGTGCATACTCTGGTCATTTTTCAAGCCATGTT

TTATCGTATCTGTTTTCTACTTTATGTGAGCAAGGTTTGCTGTCCGAGGTGTAAATATTCAACGGGAATA

AAACTGGCATGGTACTTTTTTTTTTTTTTTTGGCTCTTTCAAAGGTAATGGCCCATCGATGAGCATTTTT

AACATACTCCATAGTCTTTTCCTGTGGTGTTAGGTCTTTATTTTTATTTTTTTCCTGGGGGCTGGGGTGG

GGGTTTGTCATGGGGGAACTGCCCTTTAAATTTTAAGTGACACTACAGAAAAACACAAAAAGGTGATGGG

TTGTGTTATGCTTGTATTGAATGCTGTCTTGACATCTCTTGCCTTGTCCTCCGGTATGTTCTAAAGCTGT

GTCTGAGATCTGGATCTGCCCATCACTTTGGCTAGTGACAGGGCTAATTAATTTGCTTTATACATTTTCT

TTTACTTTCCTTTTTTCCTTTCTGGAGGCATCACATGCTGGTGCTGTGTCTTTATGAATGTTTTAACCAT

TTTCATGGTGGAAGAATTTTATATTTATGCAGTTGTACAATTTTATTTTTTTCTGCAAGAAAAAGTGTAA

TGTATGAAATAAACCAAAGTCACTTGTTTGAAAATAAATCTTTATTTTGAACTTTATAAAAAGCAATGCA

GGTACCCCATAGACTGGTGTTAAATGTTGTCTACAGTGCAAAATCCATGTTCTAACATATGTAATAATTG

CCAGGAGTACAGTGCTCTTGTTGATCTTGTATTCAGTCAGGTTAAAACAATGGACAATAAAAGAATGAAC

ACAAAAAAAAAAAAAAAAAA

USING CLUSTAL OMEGA:

Go to CLUSTALO and enter both sequences

Clustalw sequence is ready