Tugas desain vaksin dari epitope

Soal : envelope protein Mycobacterium tuberculosis (untuk vaksin Mtb)

1. Lakukan pencarian protein dari berbagai organisme tersebut di ncbi

Multiple Squence Aligment

Buka situs NCBI kemudian masukan jenis mikrobiologi yang diinginkan yaitu envelope protein
mikobacterium tuberculosis. Akan muncul tampilan seperti dibawah ini.

2. Pilih urutan asam amino yang jumlah urutan asam aminonya sama kemudian di download FASTA
nya

Pilih minimal 4 macam jenis envelope yang ditemukan dengan pilihan Panjang asam amino yang sama
dan teratas. Kemudian klik FASTA, maka akan muncul gambar seperti dibawah ini.
Urutan ke 5 protein yang ditemukan yaitu :

1. >PRI05261.1 envelope protein [Mycobacterium tuberculosis variant

microti]
MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFADAVAAECRRV
GVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWLGTHNSFNSLSDSFTVSHADS
NQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHGLGPKNANLGCTVEPLLATVLPQIANWLNAP
GHTEEVILLYLEDQLKNASAYESVVATLDQVLRRADGTSLIYRPNPARRGPQ
2. >PRI04797.1 envelope protein [Mycobacterium tuberculosis variant bovis]
MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFADAVAAECRRV
GVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWLGTHNSFNSLSDSFTVSHADS
NQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHGLGPKNANLGCTVEPLLATVLPQIANWLNAP
GHTEEVILLYLEDQLKNASAYESVVATLDQVLRRADGTSLIYRPNPARRGPQ
3. >PRH97837.1 envelope protein [Mycobacterium tuberculosis variant
africanum]
MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFADAVAAECRRV
GVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWLGTHNSFNSLSDSFTVSHADS
NQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHGLGPKNANLGCTVEPLLATVLPQIANWLNAP
GHTEEVILLYLEDQLKNASAYESVVATLDQVLRRADGTSLIYRPNPARRGPQ
4. >PRH96214.1 envelope protein [Mycobacterium tuberculosis variant caprae]
MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFADAVAAECRRV
GVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWLGTHNSFNSLSDSFTVSHADS
NQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHGLGPKNANLGCTVEPLLATVLPQIANWLNAP
GHTEEVILLYLEDQLKNASAYESVVATLDQVLRRADGTSLIYRPNPARRGPQ
5. >PRH89993.1 envelope protein [Mycobacterium tuberculosis variant
pinnipedii]
MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFADAVAAECRRV
GVPNARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWLGTHNSFNSLSDSFTVSHADS
NQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHGLGPKNANLGCTVEPLLATVLPQIANWLNAP
GHTEEVILLYLEDQLKNASAYESVVATLDQVLRRADGTSLIYRPNPARRGPQ

3. Lakukan pensejajaran (Mafft) (untuk mencari consensus)

Buka situs https://mafft.cbrc.jp/alignment/server kemudian copy seluruh hasil protein yang didapat
ke kolom “Input paste protein or DNA Squence in Fasta Format” kemudian submit, maka akan
muncul gambar seperti dibawah ini
 PRI05261.1 MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFA
PRI04797.1 MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFA
PRH97837.1 MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFA
PRH96214.1 MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFA
PRH89993.1 MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFA
************************************************************

PRI05261.1 DAVAAECRRVGVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWL
PRI04797.1 DAVAAECRRVGVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWL
PRH97837.1 DAVAAECRRVGVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWL
PRH96214.1 DAVAAECRRVGVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWL
PRH89993.1 DAVAAECRRVGVPNARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWL
*************:**********************************************

PRI05261.1 GTHNSFNSLSDSFTVSHADSNQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHG
PRI04797.1 GTHNSFNSLSDSFTVSHADSNQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHG
PRH97837.1 GTHNSFNSLSDSFTVSHADSNQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHG
PRH96214.1 GTHNSFNSLSDSFTVSHADSNQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHG
PRH89993.1 GTHNSFNSLSDSFTVSHADSNQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHG
************************************************************

PRI05261.1 LGPKNANLGCTVEPLLATVLPQIANWLNAPGHTEEVILLYLEDQLKNASAYESVVATLDQ
PRI04797.1 LGPKNANLGCTVEPLLATVLPQIANWLNAPGHTEEVILLYLEDQLKNASAYESVVATLDQ
PRH97837.1 LGPKNANLGCTVEPLLATVLPQIANWLNAPGHTEEVILLYLEDQLKNASAYESVVATLDQ
PRH96214.1 LGPKNANLGCTVEPLLATVLPQIANWLNAPGHTEEVILLYLEDQLKNASAYESVVATLDQ
PRH89993.1 LGPKNANLGCTVEPLLATVLPQIANWLNAPGHTEEVILLYLEDQLKNASAYESVVATLDQ
************************************************************

PRI05261.1 VLRRADGTSLIYRPNPARRGPQ
PRI04797.1 VLRRADGTSLIYRPNPARRGPQ
PRH97837.1 VLRRADGTSLIYRPNPARRGPQ
PRH96214.1 VLRRADGTSLIYRPNPARRGPQ
PRH89993.1 VLRRADGTSLIYRPNPARRGPQ

4. Download semua sequence kemudian simpan format FASTA nya

Setelah keluar CLUSTAL format alignment seperti gambar yang diatas klik clustal format(akan terdownload
otomatis)kemudian simpan file nya.
 Kemudian masuk ke situs https://www.ebi.ac.uk/Tools/msa/emboss_cons/ maka akan muncul
gambar seperti dibawah ini

Pada kolom “Enter or paste a protein alignment in any supported format” klik Choose File
kemudian pilih file yang sudah terdownload dari Clustal Format tadi kemudian klik submit.
Maka akan muncul gambar seperti gambar dibawah ini

>EMBOSS0001
MPRARWLQSAALMGALAVVLITAAPVAADAYQVPAPPSPTASCDVISPVAIPCVALGKFA
DAVAAECRRVGVPDARCVLPLAHRVTQAARDAYLQSWVHRTARFQDALQDPVPLRETQWL
GTHNSFNSLSDSFTVSHADSNQQLSLAQQLDIDVRALELDLHYLPRLEGHGAPGVTVCHG
LGPKNANLGCTVEPLLATVLPQIANWLNAPGHTEEVILLYLEDQLKNASAYESVVATLDQ
VLRRADGTSLIYRPNPARRGPQ
5. Cari sequence consensus
Buka situs https://services.healthtech.dtu.dk/service.php?NetCTL-1.2 untuk prediksi epitope CTL
(CYTOTOXIC T-CELL LIMFOSIT). Pada kolom “Paste a single sequence or several sequences
in FASTA format into the field below:” copi paste hasil emboss maka akan muncul gambar
seperti dibawah ini

6. copy sequence consensus

adalah hasil dari Emboss yang akan di copy paste ke NETCTL seperti gambar pada soal no 5

7. Prediksi epitope sel CTL dimana pada situs NETCTL di cari semua Allel mulai dari superserotype A1-
B62

8. Pilih yang merupakan ligan MHC class 1 (yang ada E nya)

NetCTL-1.2 predictions using MHC supertype A1. Threshold 0.750000

23 ID Sequence pep AAPVAADAY aff 0.1416 aff_rescale 0.6012 cle 0.9605 tap 3.1160 COMB 0.9011 <-E
85 ID Sequence pep VTQAARDAY aff 0.3800 aff_rescale 1.6132 cle 0.2149 tap 3.0650 COMB 1.7987 <-E
129 ID Sequence pep LSDSFTVSH aff 0.3349 aff_rescale 1.4220 cle 0.7653 tap -0.6130 COMB 1.5061 <-E
155 ID Sequence pep RALELDLHY aff 0.1763 aff_rescale 0.7484 cle 0.9609 tap 3.2860 COMB 1.0569 <-E
212 ID Sequence pep HTEEVILLY aff 0.8403 aff_rescale 3.5679 cle 0.9759 tap 2.6380 COMB 3.8462 <-E
244 ID Sequence pep RADGTSLIY aff 0.6371 aff_rescale 2.7052 cle 0.9623 tap 3.1770 COMB 3.0084 <-E
Number of MHC ligands 6 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype A2. Threshold 0.750000

11 ID Sequence pep ALMGALAVV aff 0.7795 aff_rescale 1.1619 cle 0.8583 tap 0.6970 COMB 1.3255 <-E
12 ID Sequence pep LMGALAVVL aff 0.4333 aff_rescale 0.6459 cle 0.5136 tap 1.0710 COMB 0.7764 <-E
15 ID Sequence pep ALAVVLITA aff 0.5203 aff_rescale 0.7757 cle 0.5983 tap -0.3850 COMB 0.8462 <-E
 18 ID Sequence pep VVLITAAPV aff 0.4661 aff_rescale 0.6947 cle 0.8745 tap 0.6870 COMB 0.8603 <-E
55 ID Sequence pep ALGKFADAV aff 0.4785 aff_rescale 0.7133 cle 0.4815 tap 0.3620 COMB 0.8036 <-E
93 ID Sequence pep YLQSWVHRT aff 0.7820 aff_rescale 1.1657 cle 0.5218 tap -0.5840 COMB 1.2147 <-E
104 ID Sequence pep FQDALQDPV aff 0.6330 aff_rescale 0.9435 cle 0.1115 tap 0.2510 COMB 0.9728 <-E
156 ID Sequence pep ALELDLHYL aff 0.5534 aff_rescale 0.8250 cle 0.9642 tap 1.1060 COMB 1.0249 <-E
180 ID Sequence pep GLGPKNANL aff 0.4175 aff_rescale 0.6223 cle 0.9689 tap 0.6090 COMB 0.7981 <-E
187 ID Sequence pep NLGCTVEPL aff 0.4371 aff_rescale 0.6516 cle 0.8079 tap 1.0020 COMB 0.8229 <-E
195 ID Sequence pep LLATVLPQI aff 0.7388 aff_rescale 1.1013 cle 0.9313 tap 0.4300 COMB 1.2625 <-E
199 ID Sequence pep VLPQIANWL aff 0.4283 aff_rescale 0.6385 cle 0.9624 tap 1.0310 COMB 0.8344 <-E
217 ID Sequence pep ILLYLEDQL aff 0.4506 aff_rescale 0.6718 cle 0.5352 tap 1.0620 COMB 0.8051 <-E
Number of MHC ligands 13 identified. Number of peptides 254. Protein name Sequence
NetCTL-1.2 predictions using MHC supertype A3. Threshold 0.750000
50 ID Sequence pep AIPCVALGK aff 0.4517 aff_rescale 0.8501 cle 0.6915 tap 0.7690 COMB 0.9923 <-E
107 ID Sequence pep ALQDPVPLR aff 0.3547 aff_rescale 0.6676 cle 0.9523 tap 1.4770 COMB 0.8843 <-E
176 ID Sequence pep TVCHGLGPK aff 0.4298 aff_rescale 0.8088 cle 0.5272 tap 0.6380 COMB 0.9198 <-E
212 ID Sequence pep HTEEVILLY aff 0.4498 aff_rescale 0.8466 cle 0.9759 tap 2.6380 COMB 1.1249 <-E
218 ID Sequence pep LLYLEDQLK aff 0.5747 aff_rescale 1.0816 cle 0.8734 tap 0.6840 COMB 1.2468 <-E
236 ID Sequence pep ATLDQVLRR aff 0.3162 aff_rescale 0.5951 cle 0.5984 tap 1.6720 COMB 0.7685 <-E
244 ID Sequence pep RADGTSLIY aff 0.2589 aff_rescale 0.4873 cle 0.9623 tap 3.1770 COMB 0.7905 <-E
250 ID Sequence pep LIYRPNPAR aff 0.5184 aff_rescale 0.9757 cle 0.8332 tap 1.8580 COMB 1.1936 <-E
Number of MHC ligands 8 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype A24. Threshold 0.750000

5 ID Sequence pep RWLQSAALM aff 0.4357 aff_rescale 0.9277 cle 0.1068 tap 0.9670 COMB 0.9921 <-E
96 ID Sequence pep SWVHRTARF aff 0.6148 aff_rescale 1.3092 cle 0.9639 tap 2.8630 COMB 1.5969 <-E
118 ID Sequence pep QWLGTHNSF aff 0.6795 aff_rescale 1.4469 cle 0.9697 tap 2.8220 COMB 1.7334 <-E
125 ID Sequence pep SFNSLSDSF aff 0.4931 aff_rescale 1.0499 cle 0.6455 tap 2.8020 COMB 1.2868 <-E
230 ID Sequence pep AYESVVATL aff 0.6109 aff_rescale 1.3009 cle 0.9727 tap 1.2730 COMB 1.5105 <-E
251 ID Sequence pep IYRPNPARR aff 0.2718 aff_rescale 0.5789 cle 0.8996 tap 1.8690 COMB 0.8072 <-E
Number of MHC ligands 6 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype A26. Threshold 0.750000

212 ID Sequence pep HTEEVILLY aff 0.5960 aff_rescale 1.5995 cle 0.9759 tap 2.6380 COMB 1.8778 <-E
223 ID Sequence pep DQLKNASAY aff 0.2135 aff_rescale 0.5729 cle 0.6958 tap 2.5350 COMB 0.8040 <-E
Number of MHC ligands 2 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype B7. Threshold 0.750000

1 ID Sequence pep MPRARWLQS aff 0.6215 aff_rescale 1.1991 cle 0.0766 tap -2.4560 COMB 1.0878 <-E
3 ID Sequence pep RARWLQSAA aff 0.6402 aff_rescale 1.2352 cle 0.0738 tap -0.3350 COMB 1.2295 <-E
33 ID Sequence pep VPAPPSPTA aff 0.3990 aff_rescale 0.7698 cle 0.9767 tap -0.8100 COMB 0.8758 <-E
35 ID Sequence pep APPSPTASC aff 0.5642 aff_rescale 1.0884 cle 0.2137 tap -0.3060 COMB 1.1051 <-E
38 ID Sequence pep SPTASCDVI aff 0.5195 aff_rescale 1.0023 cle 0.7076 tap 0.1020 COMB 1.1135 <-E
47 ID Sequence pep SPVAIPCVA aff 0.5714 aff_rescale 1.1023 cle 0.7112 tap -0.7000 COMB 1.1740 <-E
51 ID Sequence pep IPCVALGKF aff 0.4940 aff_rescale 0.9530 cle 0.6882 tap 2.4160 COMB 1.1771 <-E
71 ID Sequence pep GVPDARCVL aff 0.4350 aff_rescale 0.8391 cle 0.9618 tap 0.9460 COMB 1.0307 <-E
112 ID Sequence pep VPLRETQWL aff 0.3820 aff_rescale 0.7370 cle 0.8341 tap 0.5850 COMB 0.8914 <-E
164 ID Sequence pep LPRLEGHGA aff 0.5286 aff_rescale 1.0197 cle 0.1178 tap -0.8390 COMB 0.9954 <-E
209 ID Sequence pep APGHTEEVI aff 0.4624 aff_rescale 0.8921 cle 0.6556 tap 0.3520 COMB 1.0080 <-E
242 ID Sequence pep LRRADGTSL aff 0.3158 aff_rescale 0.6093 cle 0.9549 tap 1.2610 COMB 0.8156 <-E
253 ID Sequence pep RPNPARRGP aff 0.3731 aff_rescale 0.7197 cle 0.2440 tap -0.1020 COMB 0.7512 <-E
Number of MHC ligands 13 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype B8. Threshold 0.750000

3 ID Sequence pep RARWLQSAA aff 0.3655 aff_rescale 1.2482 cle 0.0738 tap -0.3350 COMB 1.2425 <-E
81 ID Sequence pep LAHRVTQAA aff 0.3183 aff_rescale 1.0868 cle 0.2005 tap -0.5940 COMB 1.0872 <-E
234 ID Sequence pep VVATLDQVL aff 0.1666 aff_rescale 0.5691 cle 0.9345 tap 1.2260 COMB 0.7706 <-E
Number of MHC ligands 3 identified. Number of peptides 254. Protein name Sequence
NetCTL-1.2 predictions using MHC supertype B27. Threshold 0.750000
4 ID Sequence pep ARWLQSAAL aff 0.5888 aff_rescale 1.5651 cle 0.9704 tap 1.6090 COMB 1.7911 <-E
68 ID Sequence pep RRVGVPDAR aff 0.5774 aff_rescale 1.5349 cle 0.6526 tap 2.0060 COMB 1.7331 <-E
242 ID Sequence pep LRRADGTSL aff 0.2948 aff_rescale 0.7837 cle 0.9549 tap 1.2610 COMB 0.9900 <-E
243 ID Sequence pep RRADGTSLI aff 0.4433 aff_rescale 1.1785 cle 0.9662 tap 1.0500 COMB 1.3759 <-E
Number of MHC ligands 4 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype B27. Threshold 0.750000

4 ID Sequence pep ARWLQSAAL aff 0.5888 aff_rescale 1.5651 cle 0.9704 tap 1.6090 COMB 1.7911 <-E
68 ID Sequence pep RRVGVPDAR aff 0.5774 aff_rescale 1.5349 cle 0.6526 tap 2.0060 COMB 1.7331 <-E
242 ID Sequence pep LRRADGTSL aff 0.2948 aff_rescale 0.7837 cle 0.9549 tap 1.2610 COMB 0.9900 <-E
243 ID Sequence pep RRADGTSLI aff 0.4433 aff_rescale 1.1785 cle 0.9662 tap 1.0500 COMB 1.3759 <-E
Number of MHC ligands 4 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype B39. Threshold 0.750000

4 ID Sequence pep ARWLQSAAL aff 0.1793 aff_rescale 0.5741 cle 0.9704 tap 1.6090 COMB 0.8001 <-E
86 ID Sequence pep TQAARDAYL aff 0.2210 aff_rescale 0.7076 cle 0.6115 tap 1.0730 COMB 0.8530 <-E
100 ID Sequence pep RTARFQDAL aff 0.1984 aff_rescale 0.6351 cle 0.4360 tap 1.1710 COMB 0.7591 <-E
121 ID Sequence pep GTHNSFNSL aff 0.1858 aff_rescale 0.5948 cle 0.9597 tap 0.8320 COMB 0.7804 <-E
136 ID Sequence pep SHADSNQQL aff 0.7484 aff_rescale 2.3959 cle 0.9772 tap 1.0790 COMB 2.5965 <-E
149 ID Sequence pep QLDIDVRAL aff 0.2582 aff_rescale 0.8268 cle 0.9630 tap 0.7620 COMB 1.0093 <-E
211 ID Sequence pep GHTEEVILL aff 0.4178 aff_rescale 1.3375 cle 0.9582 tap 0.6020 COMB 1.5113 <-E
213 ID Sequence pep TEEVILLYL aff 0.1903 aff_rescale 0.6092 cle 0.8943 tap 0.6310 COMB 0.7749 <-E
230 ID Sequence pep AYESVVATL aff 0.2595 aff_rescale 0.8308 cle 0.9727 tap 1.2730 COMB 1.0404 <-E
243 ID Sequence pep RRADGTSLI aff 0.1962 aff_rescale 0.6281 cle 0.9662 tap 1.0500 COMB 0.8256 <-E
Number of MHC ligands 10 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype B44. Threshold 0.750000

157 ID Sequence pep LELDLHYLP aff 0.3027 aff_rescale 0.7501 cle 0.4214 tap 0.1450 COMB 0.8205 <-E
167 ID Sequence pep LEGHGAPGV aff 0.3974 aff_rescale 0.9847 cle 0.9008 tap 0.1520 COMB 1.1274 <-E
192 ID Sequence pep VEPLLATVL aff 0.4862 aff_rescale 1.2049 cle 0.4249 tap 0.8760 COMB 1.3124 <-E
213 ID Sequence pep TEEVILLYL aff 0.5879 aff_rescale 1.4568 cle 0.8943 tap 0.6310 COMB 1.6225 <-E
Number of MHC ligands 4 identified. Number of peptides 254. Protein name Sequence

NetCTL-1.2 predictions using MHC supertype B58. Threshold 0.750000

100 ID Sequence pep RTARFQDAL aff 0.4265 aff_rescale 0.9612 cle 0.4360 tap 1.1710 COMB 1.0852 <-E
155 ID Sequence pep RALELDLHY aff 0.6205 aff_rescale 1.3984 cle 0.9609 tap 3.2860 COMB 1.7069 <-E
198 ID Sequence pep TVLPQIANW aff 0.5658 aff_rescale 1.2752 cle 0.9656 tap 1.1740 COMB 1.4787 <-E
212 ID Sequence pep HTEEVILLY aff 0.2143 aff_rescale 0.4829 cle 0.9759 tap 2.6380 COMB 0.7612 <-E
244 ID Sequence pep RADGTSLIY aff 0.5109 aff_rescale 1.1515 cle 0.9623 tap 3.1770 COMB 1.4547 <-E
Number of MHC ligands 5 identified. Number of peptides 254. Protein name Sequence
NetCTL-1.2 predictions using MHC supertype B62. Threshold 0.750000
12 ID Sequence pep LMGALAVVL aff 0.4087 aff_rescale 0.8113 cle 0.5136 tap 1.0710 COMB 0.9419 <-E
23 ID Sequence pep AAPVAADAY aff 0.2411 aff_rescale 0.4787 cle 0.9605 tap 3.1160 COMB 0.7786 <-E
85 ID Sequence pep VTQAARDAY aff 0.4669 aff_rescale 0.9270 cle 0.2149 tap 3.0650 COMB 1.1125 <-E
86 ID Sequence pep TQAARDAYL aff 0.5107 aff_rescale 1.0138 cle 0.6115 tap 1.0730 COMB 1.1591 <-E
142 ID Sequence pep QQLSLAQQL aff 0.3303 aff_rescale 0.6557 cle 0.6957 tap 1.2020 COMB 0.8201 <-E
155 ID Sequence pep RALELDLHY aff 0.4545 aff_rescale 0.9022 cle 0.9609 tap 3.2860 COMB 1.2106 <-E
212 ID Sequence pep HTEEVILLY aff 0.3413 aff_rescale 0.6776 cle 0.9759 tap 2.6380 COMB 0.9559 <-E
223 ID Sequence pep DQLKNASAY aff 0.4300 aff_rescale 0.8536 cle 0.6958 tap 2.5350 COMB 1.0847 <-E
234 ID Sequence pep VVATLDQVL aff 0.3032 aff_rescale 0.6019 cle 0.9345 tap 1.2260 COMB 0.8033 <-E
244 ID Sequence pep RADGTSLIY aff 0.3374 aff_rescale 0.6698 cle 0.9623 tap 3.1770 COMB 0.9730 <-E
Number of MHC ligands 10 identified. Number of peptides 254. Protein name Sequence

9. prediksi epitope sel htl