PPT 2017 T4 1 4551/3

Question 1/
Soalan 1

A group of students carried out an experiment to study the process of substances movement
through the plasma membrane. Diagram 1 shows the arrangement of apparatus used in this
experiment.

Sekumpulan pelajar telah menjalankan satu eksperimen untuk mengkaji proses pergerakan
bahan merentasi membran plasma. Rajah 1 menunjukkan susunan radas yang digunakan
dalam eksperimen itu.

Retort stand

Beaker
20%

Diagram 1/ Rajah 1

Steps for the experiment are as follows:

Langkah-langkah eksperimen adalah seperti berikut:

1. The visking tubing is soaked in water until soft. A knot is tied at one end of the visking
tubing.
Tiub visking direndam di dalam air sehingga lembut. Satu ikatan dibuat dihujung tiub
visking.

2. The visking tubing A is filled with 20 ml 20% sucrose solution using a pipette.
Tiub visking A diisi dengan 20ml 20% larutan sukrosa dengan menggunakan pipet.

3. The other end of the visking tubing is then fitted onto one end of the capillary tube
and tied with a piece of thread.
Satu lagi hujung tiub visking kemudiannya dikemaskan pada satu hujung tiub kapilari
dan diikat dengan menggunakan benang.

4. Immersed the visking tubing into a beaker of distilled water and clamped the the
capillary tube to a retort stand.
Rendamkan tiub visking ke dalam bikar yang mengandungi air suling dan kepitkan
tiub kapilari pada kaki retort.

5. Mark initial level of the sucrose solution in the capillary tube

Tanda aras awal larutan sukrosa di dalam tiub kapilari.

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6. After 20 minutes, mark the final level of the sucrose solution in the capillary tube.
Selepas 20 minit, tanda aras akhir larutan sukrosa di dalam tiub kapilari.

7. Step 1 to 7 are repeated by using 5% sucrose solution, 10% sucrose solution and
15% sucrose solution.
Langkah 1 hingga 7 diulang dengan menggunakan larutan sukrosa 5%, larutan
sukrosa 10% dan larutan sukrosa 15%.

Table 1 shows the results of level of sucrose solution after the visking tubing is immersed in
the beaker for 20 minutes
Jadual 1 menunjukkan keputusan aras larutan sukrosa selepas tiub visking direndam di
dalam bikar selama 20 minit.

Percentage of Initial level of sucrose solution in Final level of sucrose solution in

sucrose capillary tube capillary tube
solution(%)

9
20
6

6 8

15

7
5

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PPT 2017 T4 3 4551/3

10 7

1 (a) Record the final level of sucrose solution in the boxes provided in table 1.
Rekod bacaan akhir aras larutan sukrosa di dalam kotak yang disediakan di dalam
jadual 1
[3 marks/markah]

1(b)(i) State two different observations made from Table 2.

Nyatakan dua pemerhatian yang berbeza yang dibuat daripada Jadual 2.

Observation 1 / Pemerhatian 1:

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Observation 2 / Pemerhatian 2:

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[3 marks/ markah]

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Table 1
Jadual 1
PPT 2017 T4 4 4551/3

(ii) State the inference which corresponds to the observations in 1(b)(i).

Nyatakan inferens yang sepadan dengan pemerhatian di 1(b)(i).

Inference from observation 1 / Inferens daripada pemerhatian 1:

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Inference from observation 2 / Inferens daripada pemerhatian 2:

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[3 marks/markah]

(c) Complete Table 2 based on this experiment.

Lengkapkan Jadual 2 berdasarkan eksperimen ini.

Variable Particular to be implemented

Manipulated variable

Responding variable

Constant variable

[3 marks/ markah]

Table 2/Jadual 2

(e) State the hypothesis for this experiment.

Nyatakan hipotesis bagi eksperimen ini.

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[3 marks/markah]

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PPT 2017 T4 5 4551/3

(f) (i) Construct a table and record all the data collected in this experiment.
Bina satu jadual dan rekodkan semua data yang dikumpul dalam eksperimen ini.

Your table should have the following titles:

Jadual anda hendaklah mengandungi tajuk-tajuk berikut:

Concentration of sucrose solution

Kepekatan larutan sukrosa
Level of sucrose solution
Aras larutan sukrosa
Increasing level of sucrose solution
Peningkatan aras larutan sukrosa
The diffusion rate of water molecules
Kadar resapan molekul air

[3 marks/markah]

(f)(ii) Use the graph paper provided to answer this part of the question.
Using the data in 1(f)(i), draw the graph of the final level of sucrose solution against
the concentration of sucrose solution.

Gunakan kertas graf yang disediakan di halaman 9 untuk menjawab ceraian soalan
ini. Menggunakan data di 1(f)(i), lukis graf aras akhir larutan sukrosa melawan
kepekatan larutan sukrosa.
[3 marks/markah]

(e) Based on the graph in 1(f)(ii), state the relationship between the diffusion rate of water
molecule with the concentration of sucrose solution.
Explain your answer.
Berdasarkan graf di 1(f)(ii), nyatakan hubungan kadar resapan molekul air dengan
kepekatan larutan sukrosa.
Terangkan jawapan anda.
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PPT 2017 T4 6 4551/3

[3 marks/markah]

(h) This experiment is repeated by changing the content inside the visking tubing and in
the beaker. About 20ml of 20% sucrose solution is pour into the beaker and 5%
sucrose solution is filled into the visking tubing.
Predict the outcome of this experiment
Explain your answer.
Eksperimen ini diulangi dengan menukarkan kandungan di dalam tiub visking dan
di dalam bikar. Sebanyak 20ml larutan sukrosa 20% dituangkan ke dalam bikar
dan larutan sukrosa 5% diisi ke dalam tiub visking.
Ramalkan pemerhatian yang diperolehi daripada eksperimen ini.
Terangkan jawapan anda.

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[3 marks/markah]

(i) Based on the result from this experiment, what is the definition of operation for
osmosis?
Berdasarkan keputusan daripada eksperimen ini, apakah definisi operasi bagi
osmosis?

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[3 marks/markah]

(j) In Table 4, list all the materials and apparatus labeled in Diagram 1.
Dalam Jadual 4, senaraikan semua bahan dan radas yang berlabel dalam Rajah 1

Material Apparatus

Table 3/ Jadual 3
[3 marks/markah]

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PPT 2017 T4 7 4551/3

The diffusion rate of water molecule against concentration of sucrose solution

Kadar resapan molekul air melawan kepekatan larutan sukrosa

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PPT 2017 T4 8 4551/3

Question 2/
Soalan 2

Enzymes are biological catalysts which speed up biochemical reactions but remain
unchanged at the end of the reaction. Enzymes are sensitive to pH values. The rate of
enzyme reaction in an enzyme-catalysed reaction is efficient at optimum pH values.
Enzim adalah pemangkin biologi yang mempercepatkan tindakbalas biokimia tetapi tidak
berubah di akhir eksperimen. Enzim adalah sensitif kepada nilai-nilai pH. Kadar
tindakbalas enzim di dalam tindakbalas pemangkin-enzim adalah cekap pada nilai pH
yang optimum.

Design a laboratory experiment to study the effect of pH values on the rate of Pepsin
reaction.
Rekabentuk satu eksperimen dalam makmal untuk mengkaji kesan nilai pH ke atas kadar
tindakbalas Pepsin .

The planning of your experiment must include the following aspects:

Perancangan eksperimen anda hendaklah mengandungi aspek-aspek berikut:

Problem statement/ Pernyataan masalah

Hypothesis/ Hipotesis

Variables/ Pembolehubah

List of apparatus and materials/ Senarai radas dan bahan

Experimental procedure/ Prosedur eksperimen

Presentation of Data/ Persembahan Data

[17 marks/markah]

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